scholarly journals Inflammatory Stimuli Significantly Change the miRNA Profile of Human Adipose-Derived Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Zicheng Wang ◽  
Haili Li ◽  
Li Cheng ◽  
Zhongyuan Zhang ◽  
Hao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Expression Profile ◽  
Mirna Expression ◽  
Target Genes ◽  
Mirna Expression Profile ◽  
Normal Group ◽  
Differentially Expressed ◽  
Adipose Derived Stem Cells ◽  
Cellular Processes ◽  
Inflammatory Stimuli

Background. Human adipose-derived stem cells (hASCs) have been shown to have immunoregulatory properties in many studies. However, the mechanisms remain unknown. miRNAs are associated with many cellular processes, including immune responses. Thus, we hypothesized that miRNAs act as immunoregulators when hASCs are stimulated by inflammatory environments. Methods. A set of cytokines was used to stimulate the hASCs in the cytokine group, while no cytokines were used to stimulate the cells in the normal group. A microarray was used to obtain the miRNA expression profile of hASCs, and RT-PCR was used to validate the miRNAs that were differentially expressed between the two groups. Target genes were predicted using online databases, and KEGG analysis was performed to identify the pathways enriched by the target genes of all the differentially expressed miRNAs. Results. Five miRNAs were significantly upregulated, and 2 miRNAs were downregulated in the cytokine group compared with the normal group. We identified several immune-related pathways that are targeted individually or collectively by those miRNAs. Conclusion. Inflammatory stimuli changed the miRNA expression profile of hASCs. miRNAs may play a pivotal role in the immune response in hASCs and may be targets through which the immunoregulatory functions of hASCs can be enhanced.

scholarly journals miRNA Expression Profile of Saliva in Subjects of Yang Deficiency Constitution and Yin Deficiency Constitution

2018 ◽  
Vol 49 (5) ◽  
pp. 2088-2098 ◽  
Author(s):  
Yu Chen ◽  
Yanling Wu ◽  
Haiqiang Yao ◽  
Hui Luo ◽  
Bing Lin ◽  
...  
Keyword(s):  
Expression Profile ◽  
Mirna Expression ◽  
Target Genes ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Hormone Signaling ◽  
Chinese Han ◽  
Cold Intolerance ◽  
Yin Deficiency ◽  
Differentially Expressed Mirnas

Background/Aims: Based on the theory of constitution in Traditional Chinese Medicine (TCM), the Chinese Han population has been classified into nine constitutions. Of these, Yang deficiency constitution mainly exhibit cold intolerance while Yin deficiency constitution mainly exhibit heat intolerance. Some studies have been carried out to explore the modern genetic and biological basis of such constitution classification, but more remains to be done. MicroRNA (miRNA) serves as post-transcriptional regulators of gene expression and may play a role in the classification process. Here, we examined miRNA expression profile of saliva to further improve the comprehensiveness of constitution classification. Methods: Saliva was collected from Chinese Han individuals with Yang deficiency, Yin deficiency and Balanced constitutions (n=5 each), and miRNA expression profile was determined using the Human miRNA OneArray®v7. Based on 1.5 Fold change, means log2|Ratio|≥0.585 and P-value< 0.05, differentially expressed miRNA was screened. Target genes were predicted using DIANA-TarBasev7.0 and analysis of KEGG pathway was carried out using DIANA-mirPathv.3. Results: We found that 81 and 98 differentially expressed miRNAs were screened in Yang deficiency and Yin deficiency constitution, respectively. Among them, 16 miRNAs were identical and the others were unique. In addition, the target genes that are regulated by the unique miRNAs were significantly enriched in 27 and 20 signaling pathways in Yang deficiency and Yin deficiency constitution, respectively. Thyroid hormone signaling pathway is present in both constitutions. These unique miRNAs that regulated target genes of thyroid hormone signaling pathway may be associated with cold intolerance or heat intolerance. Conclusion: The results of our study show that Yang deficiency and Yin deficiency constitutions exhibit systematic differences in miRNA expression profile. Moreover, the distinct characteristics of TCM constitution may be explained, in part, by differentially expressed miRNAs.

miRNA expression profile during osteogenic differentiation of human adipose-derived stem cells

2012 ◽  
Vol 113 (3) ◽  
pp. 888-898 ◽  
Author(s):  
Zi-ji Zhang ◽  
Hao Zhang ◽  
Yan Kang ◽  
Pu-yi Sheng ◽  
Yuan-chen Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Adipose Derived Stem Cells

scholarly journals HPV16 increases the number of migratory cancer stem cells and modulates their miRNA expression profile in oropharyngeal cancer

2018 ◽  
Vol 143 (6) ◽  
pp. 1426-1439 ◽  
Author(s):  
Martin Hufbauer ◽  
Margaret Maltseva ◽  
Jeannine Meinrath ◽  
Axel Lechner ◽  
Dirk Beutner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Expression Profile ◽  
Mirna Expression ◽  
Oropharyngeal Cancer ◽  
Mirna Expression Profile

scholarly journals miRNA expression profile in mesenchymal stem cells from OA patients during osteogenic differentiation

2014 ◽  
Vol 22 ◽  
pp. S320
Author(s):  
P. Tornero-Esteban ◽  
J. Lamas ◽  
L. Rodriguez-Rodriguez ◽  
E. Villafuertes ◽  
J. Hoyas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile

scholarly journals miRNA Expression Profile and Effect of Wenxin Granule in Rats with Ligation-Induced Myocardial Infarction

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Aiming Wu ◽  
Lixia Lou ◽  
Jianying Zhai ◽  
Dongmei Zhang ◽  
Limin Chai ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Differentially Expressed ◽  
Coronary Artery Ligation ◽  
Mrna Levels ◽  
Artery Ligation ◽  
Gene Level ◽  
Differentially Expressed Mirnas

Wenxin Granule (WXKL) is a traditional Chinese medicine used for treatment of myocardial infarction (MI) and arrhythmias. However, the genomic pathological mechanisms of MI and mechanisms of WXKL are largely unknown. This study aims to investigate a comprehensive miRNA expression profile, and the predicted correlation pathways to be targeted by differentially expressed miRNAs in MI, and mechanisms of WXKL from a gene level. MI rat model was established by a coronary artery ligation surgery. miRNA expression microarrays were performed and the data were deposited in Gene Expression Omnibus (GEO number GSE95855). And, pathway analysis was performed by using the DIANA-miRPath v3.0 online tool. The expressions of miR-1, miR-133, Cx43, and Cx45 were detected by quantitative real-time PCR. It was found that 35 differentially expressed miRNAs and 23 predicted pathways, including miR-1, miR-133, and gap junction pathway, are involved in the pathogenesis of MI. And, WXKL increased the expressions of miR-1 and miR-133, while also increased the mRNA levels of Cx43 and Cx45, and, especially, recovered the Cx43/Cx45 ratio near to normal level. The results suggest that regulatory effects on miR-1, miR-133, Cx43, and Cx45 might be a possible mechanism of WXKL in the treatment of MI at the gene level.

scholarly journals miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line

2012 ◽  
Vol 28 (6) ◽  
pp. 2115-2124 ◽  
Author(s):  
YANTIAN FANG ◽  
JIANBIN XIANG ◽  
ZONGYOU CHEN ◽  
XIAODONG GU ◽  
ZHENGYANG LI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Colon Cancer Stem Cells ◽  
Sw1116 Cell

scholarly journals CD14 gene silencing alters the microRNA expression profile of RAW264.7 cells stimulated by Brucella melitensis infection

2017 ◽  
Vol 23 (5) ◽  
pp. 424-431 ◽  
Author(s):  
Hui Rong ◽  
Hanwei Jiao ◽  
Yongchang Hao ◽  
Feng Pang ◽  
Guohua Li ◽  
...  
Keyword(s):  
Immune Response ◽  
Expression Profile ◽  
Mirna Expression ◽  
Target Genes ◽  
Brucella Melitensis ◽  
Mirna Expression Profile ◽  
Macrophage Response ◽  
Knock Down ◽  
Raw264.7 Macrophages ◽  
Brucella Infection

Innate recognition of Brucella spp. is a key step in the activation of inflammation. CD14 binds PAMPs and is involved in LPS-induced pro-inflammatory cytokine release. Previously we showed that knock down of CD14 in RAW264.7 macrophages disrupted Brucella–host interactions. However, its effect on the macrophage microRNA (miRNA) expression profile, especially after stimulation by Brucella infection, is still unclear. To identify miRNAs involved in the macrophage response to Brucella infection, we performed miRNA expression profiling of CD14 knock-down RAW264.7 (224.3) macrophages infected with Brucella melitensis, and demonstrated, for the first time, that CD14 knock down significantly up-regulated the expression of mmu-miR-199a-3p and mmu-miR-183-5p in these conditions. These miRNAs have a well-characterized association with the target genes involved in immune response, inflammatory response, innate immune response, apoptosis processes, anti-apoptosis, cytokine production and cytokine-mediated signaling pathways. Among the 104 inflammation-related candidate target genes of mmu-miR-199a-3p and mmu-miR-183-5p in the 224.3+ B. melitensis group cells, the expression of the Cbl-b, a potential target of mmu-miR-199a-3p, was confirmed to be down-regulated using qRT-PCR and Western blot analysis. Our findings suggest that CD14 functions in the Brucella–host interaction may be through altered miRNA expression, and regulation of Cbl-b proteins.

Differential miRNA expression profile of bone marrow derived mesenchymal stem cells in fanconi anemia patients and healthy donors

2013 ◽  
Vol 41 (8) ◽  
pp. S61
Author(s):  
Aysen Gunel-Ozcan ◽  
Ilgin Cagnan ◽  
Ozlen Konu ◽  
Fatima Aerts Kaya ◽  
Baris Kuskonmaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Expression Profile ◽  
Fanconi Anemia ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Healthy Donors
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