miRNA expression profile during osteogenic differentiation of human adipose-derived stem cells

2012 ◽  
Vol 113 (3) ◽  
pp. 888-898 ◽  
Author(s):  
Zi-ji Zhang ◽  
Hao Zhang ◽  
Yan Kang ◽  
Pu-yi Sheng ◽  
Yuan-chen Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Adipose Derived Stem Cells

scholarly journals miRNA expression profile in mesenchymal stem cells from OA patients during osteogenic differentiation

2014 ◽  
Vol 22 ◽  
pp. S320
Author(s):  
P. Tornero-Esteban ◽  
J. Lamas ◽  
L. Rodriguez-Rodriguez ◽  
E. Villafuertes ◽  
J. Hoyas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile

scholarly journals Inflammatory Stimuli Significantly Change the miRNA Profile of Human Adipose-Derived Stem Cells

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Zicheng Wang ◽  
Haili Li ◽  
Li Cheng ◽  
Zhongyuan Zhang ◽  
Hao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Expression Profile ◽  
Mirna Expression ◽  
Target Genes ◽  
Mirna Expression Profile ◽  
Normal Group ◽  
Differentially Expressed ◽  
Adipose Derived Stem Cells ◽  
Cellular Processes ◽  
Inflammatory Stimuli

Background. Human adipose-derived stem cells (hASCs) have been shown to have immunoregulatory properties in many studies. However, the mechanisms remain unknown. miRNAs are associated with many cellular processes, including immune responses. Thus, we hypothesized that miRNAs act as immunoregulators when hASCs are stimulated by inflammatory environments. Methods. A set of cytokines was used to stimulate the hASCs in the cytokine group, while no cytokines were used to stimulate the cells in the normal group. A microarray was used to obtain the miRNA expression profile of hASCs, and RT-PCR was used to validate the miRNAs that were differentially expressed between the two groups. Target genes were predicted using online databases, and KEGG analysis was performed to identify the pathways enriched by the target genes of all the differentially expressed miRNAs. Results. Five miRNAs were significantly upregulated, and 2 miRNAs were downregulated in the cytokine group compared with the normal group. We identified several immune-related pathways that are targeted individually or collectively by those miRNAs. Conclusion. Inflammatory stimuli changed the miRNA expression profile of hASCs. miRNAs may play a pivotal role in the immune response in hASCs and may be targets through which the immunoregulatory functions of hASCs can be enhanced.

scholarly journals HPV16 increases the number of migratory cancer stem cells and modulates their miRNA expression profile in oropharyngeal cancer

2018 ◽  
Vol 143 (6) ◽  
pp. 1426-1439 ◽  
Author(s):  
Martin Hufbauer ◽  
Margaret Maltseva ◽  
Jeannine Meinrath ◽  
Axel Lechner ◽  
Dirk Beutner ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Expression Profile ◽  
Mirna Expression ◽  
Oropharyngeal Cancer ◽  
Mirna Expression Profile

scholarly journals miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line

2012 ◽  
Vol 28 (6) ◽  
pp. 2115-2124 ◽  
Author(s):  
YANTIAN FANG ◽  
JIANBIN XIANG ◽  
ZONGYOU CHEN ◽  
XIAODONG GU ◽  
ZHENGYANG LI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Colon Cancer ◽  
Cancer Stem Cells ◽  
Cell Line ◽  
Expression Profile ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Colon Cancer Stem Cells ◽  
Sw1116 Cell

scholarly journals Identification and Characterization of Long Non-Coding RNAs in Osteogenic Differentiation of Human Adipose-Derived Stem Cells

2017 ◽  
Vol 42 (3) ◽  
pp. 1037-1050 ◽  
Author(s):  
Guangxin Huang ◽  
Yan Kang ◽  
Zhiyu Huang ◽  
Zhiqi Zhang ◽  
Fangang Meng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Expression Profile ◽  
Expression Profiles ◽  
Stem Cell Differentiation ◽  
Adipose Derived Stem Cells ◽  
Bioinformatics Analyses ◽  
Human Ascs ◽  
Global Expression Profile

Background/Aims: Long noncoding RNAs (lncRNAs) play important roles in stem cell differentiation. However, their role in osteogenesis of human adipose-derived stem cells (ASCs), a promising cell source for bone regeneration, remains unknown. Here, we investigated the expression profile and potential roles of lncRNAs in osteogenic differentiation of human ASCs. Methods: Human ASCs were induced to differentiate into osteoblasts in vitro, and the expression profiles of lncRNAs and mRNAs in undifferentiated and osteogenic differentiated ASCs were obtained by microarray. Bioinformatics analyses including subgroup analysis, gene ontology analysis, pathway analysis and co-expression network analysis were performed. The function of lncRNA H19 was determined by in vitro knockdown and overexpression. Quantitative reverse transcription polymerase chain reaction was utilized to examine the expression of selected genes. Results: We identified 1,460 upregulated and 1,112 downregulated lncRNAs in osteogenic differentiated human ASCs as compared with those of undifferentiated cells (Fold change ≥ 2.0, P < 0.05). Among these, 94 antisense lncRNAs, 85 enhancer-like lncRNAs and 160 lincRNAs were further recognized. We used 12 lncRNAs and 157 mRNAs to comprise a coding-non-coding gene expression network. Additionally, silencing of H19 caused a significantly increase in expression of osteogenesis-related genes, including ALPL and RUNX2, while a decrease was observed after H19 overexpression. Conclusion: This study revealed for the first time the global expression profile of lncRNAs involved in osteogenic differentiation of human ASCs and provided a foundation for future investigations of lncRNA regulation of human ASC osteogenesis.

Differential miRNA expression profile of bone marrow derived mesenchymal stem cells in fanconi anemia patients and healthy donors

2013 ◽  
Vol 41 (8) ◽  
pp. S61
Author(s):  
Aysen Gunel-Ozcan ◽  
Ilgin Cagnan ◽  
Ozlen Konu ◽  
Fatima Aerts Kaya ◽  
Baris Kuskonmaz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Expression Profile ◽  
Fanconi Anemia ◽  
Mirna Expression ◽  
Mirna Expression Profile ◽  
Healthy Donors
Sign in / Sign up

Export Citation Format

Share Document