scholarly journals Successful Low-Cost Scaffold-Free Cartilage Tissue Engineering Using Human Cartilage Progenitor Cell Spheroids Formed by Micromolded Nonadhesive Hydrogel

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Mellannie P. Stuart ◽  
Renata A. M. Matsui ◽  
Matheus F. S. Santos ◽  
Isis Côrtes ◽  
Mayra S. Azevedo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cell ◽  
Low Cost ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Human Cartilage ◽  
Viable Cells ◽  
Human Nasal Septum ◽  
First Time ◽  
Cartilage Construct

The scaffold-free tissue engineering using spheroids is pointed out as an approach for optimizing the delivery system of cartilage construct. In this study, we aimed to evaluate the micromolded nonadhesive hydrogel (MicroTissues®) for spheroid compaction (2-day culture) and spontaneous chondrogenesis (21-day culture) using cartilage progenitors cells (CPCs) from human nasal septum without chondrogenic stimulus. CPC spheroids showed diameter stability (486 μm ± 65), high percentage of viable cells (88.1 ± 2.1), and low percentage of apoptotic cells (2.3%). After spheroid compaction, the synthesis of TGF-β1, TGF-β2, and TGF-β3 was significantly higher compared to monolayer (p<0.005). Biomechanical assay revealed that the maximum forces applied to spheroids after chondrogenesis were 2.6 times higher than for those cultured for 2 days. After spontaneous chondrogenesis, CPC spheroids were entirely positive for N-cadherin, collagen type II and type VI, and aggrecan and chondroitin sulfate. Comparing to monolayer, the expression of SOX5 and SOX6 genes analyzed by qPCR was significantly upregulated (p<0.01). Finally, we observed the capacity of CPC spheroids starting to fuse. To the best of our knowledge, this is the first time in the scientific literature that human CPC spheroids were formed by micromolded nonadhesive hydrogel, achieving a successful scaffold-free cartilage engineering without chondrogenic stimulus (low cost).

Different Effects of PLGA and Chitosan Scaffolds on Human Cartilage Tissue Engineering

2007 ◽  
Vol 18 (6) ◽  
pp. 1249-1258 ◽  
Author(s):  
Young Hoon Jeon ◽  
Jin Hyun Choi ◽  
Joo Kyung Sung ◽  
Taek Kyun Kim ◽  
Byung Chae Cho ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Human Cartilage ◽  
Chitosan Scaffolds

scholarly journals Expansion and Redifferentiation of Chondrocytes from Osteoarthritic Cartilage: Cells for Human Cartilage Tissue Engineering

2009 ◽  
Vol 15 (11) ◽  
pp. 3513-3523 ◽  
Author(s):  
Nancy D. Hsieh-Bonassera ◽  
Iwen Wu ◽  
Jonathan K. Lin ◽  
Barbara L. Schumacher ◽  
Albert C. Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Osteoarthritic Cartilage ◽  
Human Cartilage ◽  
Cartilage Cells

Human Cartilage Tissue Engineering with Pluronic and Cultured Chondrocyte Sheet

2007 ◽  
Vol 342-343 ◽  
pp. 89-92 ◽  
Author(s):  
Jae Ho Jeong ◽  
Y.M. Moon ◽  
S.O. Kim ◽  
S.S. Yun ◽  
Hong In Shin
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cartilage Tissue Engineering ◽  
Type Ii Collagen ◽  
New Method ◽  
Cartilage Tissue ◽  
Type Ii ◽  
Human Cartilage ◽  
Covered Group ◽  
Chondrocyte Sheet

Despite many outstanding research works on cartilage tissue engineering, actual clinical application is not quite successful because of the absorption and progressive distortion of tissue engineered cartilage. We have developed a new method of cartilage tissue engineering comprising chondrocyte mixed Pluronic F-127 and cultured chondrocyte cell sheet which entirely cover the cell-Pluronic complex. We believe the addition of cultured chondrocyte cell sheet enhances the efficacy of chondrogenesis in vivo. Human ear cartilage piece was enzymatically dissociated and chondrocyte suspension was acquired. Chondrocytes were cultured and expanded as the routine manner. Cultured chondrocytes were plated in high-density monolayer and cultured with Chondrogenic media in 5% CO2 incubator. After 3 weeks of culture, chondrocyte cell sheet was formed and complete single sheet of chondrocyte could be harvested by gentle manipulation of culture plate with a cell scraper. Chondrocyte-Pluronic complex was established by mixing 1x 106 cells with 0.5 of Pluronic F- 127. Chondrocyte-Pluronic complex was completely covered with a sheet of cultured chondrocyte. The completed tissue engineered constructs were implanted into the subcutaneous tissue pocket of nude mice on the back. Tissue engineered constructs without cultured cell sheet were used as control. Samples were harvested at 8 weeks postoperatively and they were subjected to histological analysis and assayed for glycosaminoglycan (GAG), and type II collagen. Grossly, the size of cartilage specimen of cultured chondrocyte cell sheet covered group was larger than that of the control. On histologic examination, the specimen of cultured chondrocyte cell sheet covered group showed lacunae-containing cells embedded in a basophilic matrix. The chondrocyte cell sheet covered group specimen resembled mature or immature cartilage. The result of measurement of GAG and type II collagen of cartilage specimen of cultured chondrocyte sheet covered group was higher than that of the control. In conclusion, the new method of cartilage tissue engineering using chondrocyte cell sheet seems to be an effective method providing higher cartilage tissue gain and reliable success rate for cartilage tissue engineering.

scholarly journals Gelatin Microsphere for Cartilage Tissue Engineering: Current and Future Strategies

Polymers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2404
Author(s):  
Shamsul Bin Sulaiman ◽  
Ruszymah Binti Haji Idrus ◽  
Ng Min Hwei
Keyword(s):  
Tissue Engineering ◽  
Delivery System ◽  
Targeted Delivery ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
High Tendency ◽  
Gelatin Microsphere ◽  
Defect Sites ◽  
Attractive Option ◽  
Cartilage Construct

The gelatin microsphere (GM) provides an attractive option for tissue engineering due to its versatility, as reported by various studies. This review presents the history, characteristics of, and the multiple approaches to, the production of GM, and in particular, the water in oil emulsification technique. Thereafter, the application of GM as a drug delivery system for cartilage diseases is introduced. The review then focusses on the emerging application of GM as a carrier for cells and biologics, and biologics delivery within a cartilage construct. The influence of GM on chondrocytes in terms of promoting chondrocyte proliferation and chondrogenic differentiation is highlighted. Furthermore, GM seeded with cells has been shown to have a high tendency to form aggregates; hence the concept of using GM seeded with cells as the building block for the formation of a complex tissue construct. Despite the advancement in GM research, some issues must still be addressed, particularly the improvement of GM’s ability to home to defect sites. As such, the strategy of intraarticular injection of GM seeded with antibody-coated cells is proposed. By addressing this in future studies, a better-targeted delivery system, that would result in more effective intervention, can be achieved.

scholarly journals Kartogenin Enhances Chondrogenic Differentiation of MSCs in 3D Tri-Copolymer Scaffolds and the Self-Designed Bioreactor System

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 115
Author(s):  
Ching-Yun Chen ◽  
Chunching Li ◽  
Cherng-Jyh Ke ◽  
Jui-Sheng Sun ◽  
Feng-Huei Lin
Keyword(s):  
Tissue Engineering ◽  
Chondrogenic Differentiation ◽  
Cartilage Damage ◽  
Cartilage Tissue Engineering ◽  
3D Culture ◽  
Cartilage Tissue ◽  
Great Promise ◽  
Clinical Consequence ◽  
Human Cartilage

Human cartilage has relatively slow metabolism compared to other normal tissues. Cartilage damage is of great clinical consequence since cartilage has limited intrinsic healing potential. Cartilage tissue engineering is a rapidly emerging field that holds great promise for tissue function repair and artificial/engineered tissue substitutes. However, current clinical therapies for cartilage repair are less than satisfactory and rarely recover full function or return the diseased tissue to its native healthy state. Kartogenin (KGN), a small molecule, can promote chondrocyte differentiation both in vitro and in vivo. The purpose of this research is to optimize the chondrogenic process in mesenchymal stem cell (MSC)-based chondrogenic constructs with KGN for potential use in cartilage tissue engineering. In this study, we demonstrate that KGN treatment can promote MSC condensation and cell cluster formation within a tri-copolymer scaffold. Expression of Acan, Sox9, and Col2a1 was significantly up-regulated in three-dimensional (3D) culture conditions. The lacuna-like structure showed active deposition of type II collagen and aggrecan deposition. We expect these results will open new avenues for the use of small molecules in chondrogenic differentiation protocols in combination with scaffolds, which may yield better strategies for cartilage tissue engineering.

scholarly journals Neural Crest-Derived Chondrocytes Isolation for Tissue Engineering in Regenerative Medicine

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 962
Author(s):  
Monica Salamone ◽  
Salvatrice Rigogliuso ◽  
Aldo Nicosia ◽  
Marcello Tagliavia ◽  
Simona Campora ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Enzymatic Digestion ◽  
Cartilage Tissue ◽  
Cartilage Defects ◽  
Nasal Cartilage ◽  
Tissue Dissociation ◽  
In Vitro Expansion ◽  
First Time

Chondrocyte transplantation has been successfully tested and proposed as a clinical procedure aiming to repair articular cartilage defects. However, the isolation of chondrocytes and the optimization of the enzymatic digestion process, as well as their successful in vitro expansion, remain the main challenges in cartilage tissue engineering. In order to address these issues, we investigated the performance of recombinant collagenases in tissue dissociation assays with the aim of isolating chondrocytes from bovine nasal cartilage in order to establish the optimal enzyme blend to ensure the best outcomes of the overall procedure. We show, for the first time, that collagenase H activity alone is required for effective cartilage digestion, resulting in an improvement in the yield of viable cells. The extracted chondrocytes proved able to grow and activate differentiation/dedifferentiation programs, as assessed by morphological and gene expression analyses.

Human Cartilage Tissue Engineering Using Type I Collagen/Heparan Sulfate Scaffolds

2015 ◽  
Vol 03 (02) ◽  
Author(s):  
C. Sanjurjo Rodríguez ◽  
A.H. Martínez Sánchez ◽  
T. Hermida Gómez ◽  
I. Fuentes Boquete
Keyword(s):  
Tissue Engineering ◽  
Heparan Sulfate ◽  
Type I Collagen ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Type I ◽  
Human Cartilage
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