scholarly journals Biocompatibility and Toxicity of Polylactic Acid/Ferrosoferric Oxide Nanomagnetic Microsphere

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Hongzhao Xiang ◽  
Yuanhua Mu ◽  
Chengbo Hu ◽  
Xiaobing Luo
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Polylactic Acid ◽  
Ferric Oxide ◽  
Micronucleus Test ◽  
Drug Carrier ◽  
Liver Cells ◽  
Precipitation Method ◽  
Liver Cancer Cells ◽  
Targeted Drug

Magnetic targeted drugs delivery system (MTDDS) is a new targeted drug system, which can greatly reduce the dosage and improve the therapeutic efficiency of medicine. Currently superparamagnetic ferric oxide plays important function as targeted drug in the treatment of tumors, but cytotoxicity was still regarded as side effect in the process of drug. In this paper, we take advantage of drug carrier (ferric oxide) toxicity controlling cancer cell growth in cancer treatment, increasing targeted drug efficiency. We applied the modified chemical precipitation method to prepare polylactic acid (PLA) coated high-purity superparamagnetic Fe3O4 nanoparticles for targeted drug, characterized PLA/Fe3O4 microspheres physical and chemical properties, and then investigated cytotoxicity influence of PLA/Fe3O4 nanomagnetic microspheres as carrier for normal liver cells (7701) and liver cancer cells (HePG2) in different concentration; results of MTT and hemolysis and micronucleus test showed that carrier restrained the growth of HePG2 in special concentration, meanwhile the proliferation rate of liver cells was not affected. The study demonstrates that compared with liver cell, liver cancer cells (HepG2) are easy to be disturbed by PLA/Fe3O4 nanomagnetic microsphere, which have higher sensitivity and absorption ability. We hope to take advantage of the susceptible property of cancer cells for carriers to improve targeted drug function.

In vitro study of doxorubicin-loaded thermo- and pH-tunable carriers for targeted drug delivery to liver cancer cells

2021 ◽  
Vol 104 ◽  
pp. 93-105
Author(s):  
Sikhumbuzo Charles Kunene ◽  
Kuen-Song Lin ◽  
Meng-Tzu Weng ◽  
Maria Janina Carrera Espinoza ◽  
Chun-Ming Wu
Keyword(s):  
Drug Delivery ◽  
Liver Cancer ◽  
Cancer Cells ◽  
Targeted Drug Delivery ◽  
In Vitro Study ◽  
Vitro Study ◽  
Liver Cancer Cells ◽  
Targeted Drug

A study of structural differences between liver cancer cells and normal liver cells using FTIR spectroscopy

2015 ◽  
Vol 1099 ◽  
pp. 18-23 ◽  
Author(s):  
Daping Sheng ◽  
Fangcheng Xu ◽  
Qiang Yu ◽  
Tingting Fang ◽  
Junjun Xia ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Normal Liver ◽  
Liver Cells ◽  
Liver Cancer Cells ◽  
Structural Differences

Combination of ZnO Nanoparticle with Marine Sponge Derived Dipeptide for Enhanced Anticancer Efficacy in Liver Cancer Cells and their Toxicity Evaluation on Embryonic Zebrafish

2020 ◽  
Vol 16 ◽  
Author(s):  
Gayathri Karanam ◽  
Arumugam Madan Kumar ◽  
Chinmai Sriamulya Yerukalapudi ◽  
Nagabhishek Sirpu Natesh ◽  
Rajender Boddula ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Marine Sponge ◽  
Zno Nanoparticles ◽  
Normal Liver ◽  
Liver Cells ◽  
Zebrafish Embryos ◽  
Zno Nanoparticle ◽  
Anticancer Efficacy ◽  
Liver Cancer Cells

Background: Nanomaterials-based cancer therapy plays a significant role in increasing the therapeutic efficiency of anticancer drugs, reducing side effects and targeted delivery of the drug payloads. The present study was aimed to enhance the anticancer effect of a novel dipeptide isolated from marine sponge associated Bacillus pumilus AMK1 by formulating with Zinc oxide (ZnO) nanoparticles for the effective treatment against HepG2 liver cancer cells. Methods: The ZnO nanoparticles were synthesized by chemical method and size of the nanoparticle was characterized by Scanning electron microscope, X-Ray diffraction and Fourier-transform infrared spectroscopy. Further, The ZnO nanoparticles were conjugated with the isolated dipeptide and evaluated for anticancer activity. In addition, distinct morphological changes were observed by performing apoptotic staining methods such as propidium iodide staining and acridine orange/ ethidium bromide staining. Furthermore, embryotoxic and teratogenic effects of conjugated dipeptide on the development of zebrafish embryo were investigated in this study. Results: It was observed that conjugated dipeptide showed enhanced cytotoxicity against HepG2 liver cancer cells without any toxic effect on normal liver cells. ZnO with dipeptide showed a significant higher apoptosis of liver cancer cells with around 19% in early apoptosis and 53% in late apoptosis stage. The obtained results suggest that ZnO nanoparticle conjugated dipeptide initiated cytotoxicity through apoptotic death in HepG2 cells. The embryotoxic studies in zebrafish embryos revealed the LC50 197.0 µg/mL. These findings suggest that conjugated dipeptide affected the development of zebrafish embryos only at relatively higher concentrations. Conclusion: The experimental results demonstrate that Zno nanoparticle conjugated dipeptide has the potential to improve anticancer efficacy against liver cancer cells by inducing apoptosis in cancer cells without effecting normal liver cells.

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