13,28-Epoxy triterpenoid saponins from Ardisia japonica selectively inhibit proliferation of liver cancer cells without affecting normal liver cells

2012 ◽  
Vol 22 (19) ◽  
pp. 6120-6125 ◽  
Author(s):  
Qin Li ◽  
Wei Li ◽  
Lin-Ping Hui ◽  
Chen-Yang Zhao ◽  
Lin He ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Normal Liver ◽  
Liver Cells ◽  
Triterpenoid Saponins ◽  
Liver Cancer Cells

A study of structural differences between liver cancer cells and normal liver cells using FTIR spectroscopy

2015 ◽  
Vol 1099 ◽  
pp. 18-23 ◽  
Author(s):  
Daping Sheng ◽  
Fangcheng Xu ◽  
Qiang Yu ◽  
Tingting Fang ◽  
Junjun Xia ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Ftir Spectroscopy ◽  
Cancer Cells ◽  
Normal Liver ◽  
Liver Cells ◽  
Liver Cancer Cells ◽  
Structural Differences

Combination of ZnO Nanoparticle with Marine Sponge Derived Dipeptide for Enhanced Anticancer Efficacy in Liver Cancer Cells and their Toxicity Evaluation on Embryonic Zebrafish

2020 ◽  
Vol 16 ◽  
Author(s):  
Gayathri Karanam ◽  
Arumugam Madan Kumar ◽  
Chinmai Sriamulya Yerukalapudi ◽  
Nagabhishek Sirpu Natesh ◽  
Rajender Boddula ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Marine Sponge ◽  
Zno Nanoparticles ◽  
Normal Liver ◽  
Liver Cells ◽  
Zebrafish Embryos ◽  
Zno Nanoparticle ◽  
Anticancer Efficacy ◽  
Liver Cancer Cells

Background: Nanomaterials-based cancer therapy plays a significant role in increasing the therapeutic efficiency of anticancer drugs, reducing side effects and targeted delivery of the drug payloads. The present study was aimed to enhance the anticancer effect of a novel dipeptide isolated from marine sponge associated Bacillus pumilus AMK1 by formulating with Zinc oxide (ZnO) nanoparticles for the effective treatment against HepG2 liver cancer cells. Methods: The ZnO nanoparticles were synthesized by chemical method and size of the nanoparticle was characterized by Scanning electron microscope, X-Ray diffraction and Fourier-transform infrared spectroscopy. Further, The ZnO nanoparticles were conjugated with the isolated dipeptide and evaluated for anticancer activity. In addition, distinct morphological changes were observed by performing apoptotic staining methods such as propidium iodide staining and acridine orange/ ethidium bromide staining. Furthermore, embryotoxic and teratogenic effects of conjugated dipeptide on the development of zebrafish embryo were investigated in this study. Results: It was observed that conjugated dipeptide showed enhanced cytotoxicity against HepG2 liver cancer cells without any toxic effect on normal liver cells. ZnO with dipeptide showed a significant higher apoptosis of liver cancer cells with around 19% in early apoptosis and 53% in late apoptosis stage. The obtained results suggest that ZnO nanoparticle conjugated dipeptide initiated cytotoxicity through apoptotic death in HepG2 cells. The embryotoxic studies in zebrafish embryos revealed the LC50 197.0 µg/mL. These findings suggest that conjugated dipeptide affected the development of zebrafish embryos only at relatively higher concentrations. Conclusion: The experimental results demonstrate that Zno nanoparticle conjugated dipeptide has the potential to improve anticancer efficacy against liver cancer cells by inducing apoptosis in cancer cells without effecting normal liver cells.

The role of microRNA-9 in hepatocellular oncogenesis.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 168-168
Author(s):  
Alexandra Drakaki ◽  
Maria Hatziapostolou ◽  
Christos Polytarchou ◽  
Dimitrios Iliopoulos
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Cancer Patients ◽  
Expression Analysis ◽  
Therapeutic Potential ◽  
Normal Liver ◽  
Metastatic Potential ◽  
Mrna Levels ◽  
Matrigel Invasion ◽  
Liver Cancer Cells

168 Background: Hepatocellular carcinoma (HCC) is the main type of liver cancer. MicroRNAs are non-coding RNAs that have been involved in the pathogenesis of different cancer types. Our aim was to identify microRNAs that have functional and clinical significance in liver oncogenesis and understand how manipulation of their levels could have a therapeutic potential. Methods: MicroRNA expression analysis was performed in 38 HCC tissues and 25 normal liver tissues. A microRNA library (318 microRNAs) was transfected in SNU-449 liver cancer cells and invasiveness was measured 24 h later by a matrigel invasion assay. Cell growth and matrigel invasion assays were performed in SNU-449 cells that were transfected with miR-9 or antisense-miR-9 (20nM). TargetScan algorithm was used to identify miR-9 direct downstream target genes. The mRNA levels of PPARalpha, and E-cadherin were assessed by real-time PCR 48h after miR-9 overexpression and down-regulation in SNU-449 cells. Results: MicroRNA expression analysis in 38 HCCs and 25 normal liver tissues identified a 25-microRNA signature of HCC. Integration of the library screen and patient data revealed that miR-9 has clinical and functional relevance for liver cancer. Specifically, we found that miR-9 increases 2.7-fold the invasiveness and the growth of SNU-449 cells, 48h post transfection. Bioinformatic analysis revealed that miR-9 has a binding site in the 3’UTR of PPARalpha gene. MiR-9 overexpression inhibited 35% PPARalpha 3’UTR luciferase activity and 60% mRNA levels, while miR-9 down-regulation led to 75% increased PPARalpha mRNA levels. These data suggest that miR-9 targets directly the 3'UTR of PPARalpha and regulates its expression levels in liver cancer cells. In addition, miR-9 overexpression led to 95% suppression of E-cadherin mRNA levels in SNU-449 cells, thus increasing their metastatic potential. Conclusions: We found that miR-9 is highly overexpressed in liver cancer patients and its up-regulation increases the growth and metastatic potential of liver cancer cells. Overall these data suggest that miR-9 is a novel oncogene involved in liver oncogenesis and its suppression could have therapeutic potential in liver cancer patients.

scholarly journals Biocompatibility and Toxicity of Polylactic Acid/Ferrosoferric Oxide Nanomagnetic Microsphere

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Hongzhao Xiang ◽  
Yuanhua Mu ◽  
Chengbo Hu ◽  
Xiaobing Luo
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Polylactic Acid ◽  
Ferric Oxide ◽  
Micronucleus Test ◽  
Drug Carrier ◽  
Liver Cells ◽  
Precipitation Method ◽  
Liver Cancer Cells ◽  
Targeted Drug

Magnetic targeted drugs delivery system (MTDDS) is a new targeted drug system, which can greatly reduce the dosage and improve the therapeutic efficiency of medicine. Currently superparamagnetic ferric oxide plays important function as targeted drug in the treatment of tumors, but cytotoxicity was still regarded as side effect in the process of drug. In this paper, we take advantage of drug carrier (ferric oxide) toxicity controlling cancer cell growth in cancer treatment, increasing targeted drug efficiency. We applied the modified chemical precipitation method to prepare polylactic acid (PLA) coated high-purity superparamagnetic Fe3O4 nanoparticles for targeted drug, characterized PLA/Fe3O4 microspheres physical and chemical properties, and then investigated cytotoxicity influence of PLA/Fe3O4 nanomagnetic microspheres as carrier for normal liver cells (7701) and liver cancer cells (HePG2) in different concentration; results of MTT and hemolysis and micronucleus test showed that carrier restrained the growth of HePG2 in special concentration, meanwhile the proliferation rate of liver cells was not affected. The study demonstrates that compared with liver cell, liver cancer cells (HepG2) are easy to be disturbed by PLA/Fe3O4 nanomagnetic microsphere, which have higher sensitivity and absorption ability. We hope to take advantage of the susceptible property of cancer cells for carriers to improve targeted drug function.

Panobinostat treatment determines oncogenic miRNAs down-regulation in liver cancer cells

2012 ◽  
Vol 50 (01) ◽  
Author(s):  
A Henrici ◽  
R Montalbano ◽  
K Quint ◽  
M Ocker ◽  
P Di Fazio
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Down Regulation ◽  
Liver Cancer Cells ◽  
Oncogenic Mirnas

HBV-Encoded miR-2 Functions as an Oncogene by Downregulating TRIM35 But Upregulating RAN in Liver Cancer Cells

2019 ◽  
Author(s):  
Lili Yao ◽  
Zhen-hua Sui ◽  
Yan-Kun Liu ◽  
Hong Xie ◽  
Hui-jie Gao ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Liver Cancer Cells

Titanium Oxide Nanoparticles Improve the Chemotherapeutic Action of Erlotinib in Liver Cancer Cells

2020 ◽  
Vol 16 (4) ◽  
pp. 337-343
Author(s):  
Shaimaa E. Abdel-Ghany ◽  
Eman El-Sayed ◽  
Nour Ashraf ◽  
Nada Mokhtar ◽  
Amany Alqosaibi ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Titanium Oxide ◽  
Phase Distribution ◽  
Oxide Nanoparticles ◽  
Titanium Oxide Nanoparticles ◽  
Liver Cancer Cells ◽  
M Phase ◽  
Apoptosis Detection ◽  
Novel Method

Background: Hepatocellular carcinoma is the second leading cause of cancer-related deaths among other types of cancer due to lack of effective treatments and late diagnosis. Nanocarriers represent a novel method to deliver chemotherapeutic drugs, enhancing their bioavailability and stability. Methods: In the present study, we loaded gold nanoparticles (AuNPs) and titanium oxide nanoparticles (TiO2NPs) with ERL to investigate the efficiency of the formed composite in inducing apoptosis in HepG2 liver cancer cells. Cytotoxicity was assessed using MTT assay and cell phase distribution was assessed by flow cytometry along with apoptosis detection. Results: Data obtained indicated the efficiency of the formed composite to significantly induce cell death and arrest cell cycle and G2/M phase. IRF4 was downregulated after treatment with loaded ERL. Conclusion: Our data showed that loading ERL on TiO2NPs was more efficient than AuNPs. However, both nanocarriers were efficient compared with control.

Precise Electrical Detection of Curcumin Cytotoxicity in Human Liver Cancer Cells

2021 ◽  
Author(s):  
Novi Angeline ◽  
Sung-Sik Choo ◽  
Cheol-Hwi Kim ◽  
Suk Ho Bhang ◽  
Tae-Hyung Kim
Keyword(s):  
Liver Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Electrical Detection ◽  
Liver Cancer Cells ◽  
Human Liver Cancer ◽  
Human Liver Cancer Cells
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