scholarly journals Electrical Stimulation for Wound-Healing: Simulation on the Effect of Electrode Configurations

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yung-Shin Sun
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Electric Fields ◽  
Healing Process ◽  
Three Dimensional ◽  
Electrode Configuration ◽  
Wound Healing Process ◽  
Wound Model ◽  
Underlying Mechanisms ◽  
Three Dimensional Finite Element

Endogenous electric field is known to play important roles in the wound-healing process, mainly through its effects on protein synthesis and cell migration. Many clinical studies have demonstrated that electrical stimulation (ES) with steady direct currents is beneficial to accelerating wound-healing, even though the underlying mechanisms remain unclear. In the present study, a three-dimensional finite element wound model was built to optimize the electrode configuration in ES. Four layers of the skin, stratum corneum, epidermis, dermis, and subcutis, with defined thickness and electrical properties were modeled. The main goal was to evaluate the distributions of exogenous electric fields delivered with direct current (DC) stimulation using different electrode configurations such as sizes and positions. Based on the results, some guidelines were obtained in designing the electrode configuration for applications of clinical ES.

scholarly journals Loss of Diacylglycerol Kinase α Enhances Macrophage Responsiveness

2021 ◽  
Vol 12 ◽  
Author(s):  
Laryssa C. Manigat ◽  
Mitchell E. Granade ◽  
Suchet Taori ◽  
Charlotte Anne Miller ◽  
Luke R. Vass ◽  
...  
Keyword(s):  
Wound Healing ◽  
T Cell ◽  
Healing Process ◽  
Cell Activity ◽  
Regulatory Function ◽  
Burned Area ◽  
Wound Healing Process ◽  
Wound Model ◽  
Complex Wound

The diacylglycerol kinases (DGKs) are a family of enzymes responsible for the conversion of diacylglycerol (DAG) to phosphatidic acid (PA). In addition to their primary function in lipid metabolism, DGKs have recently been identified as potential therapeutic targets in multiple cancers, including glioblastoma (GBM) and melanoma. Aside from its tumorigenic properties, DGKα is also a known promoter of T-cell anergy, supporting a role as a recently-recognized T cell checkpoint. In fact, the only significant phenotype previously observed in Dgka knockout (KO) mice is the enhancement of T-cell activity. Herein we reveal a novel, macrophage-specific, immune-regulatory function of DGKα. In bone marrow-derived macrophages (BMDMs) cultured from wild-type (WT) and KO mice, we observed increased responsiveness of KO macrophages to diverse stimuli that yield different phenotypes, including LPS, IL-4, and the chemoattractant MCP-1. Knockdown (KD) of Dgka in a murine macrophage cell line resulted in similar increased responsiveness. Demonstrating in vivo relevance, we observed significantly smaller wounds in Dgka-/- mice with full-thickness cutaneous burns, a complex wound healing process in which macrophages play a key role. The burned area also demonstrated increased numbers of macrophages. In a cortical stab wound model, Dgka-/- brains show increased Iba1+ cell numbers at the needle track versus that in WT brains. Taken together, these findings identify a novel immune-regulatory checkpoint function of DGKα in macrophages with potential implications for wound healing, cancer therapy, and other settings.

Pulse Voltage Electrical Stimulation for Bacterial Inactivation and Wound Healing in Mice with Diabetes

2022 ◽  
Author(s):  
Mokhamad Tirono ◽  
Farid Samsu Hananto ◽  
Ahmad Abtokhi
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Treatment Time ◽  
Healing Process ◽  
Anaerobic Bacteria ◽  
Pulse Voltage ◽  
Wound Healing Process ◽  
Bacterial Inactivation ◽  
Wound Area ◽  
Treatment Of Wounds

Background: Treatment of wounds in diabetes often gets less than perfect healing. One of the reasons for the difficulty in treating wounds in diabetes is the growth of aerobic and anaerobic bacteria. This study aims to determine the pulse voltage and treatment time that can optimally inactivate bacteria, and their effect on wound healing in mice suffering from diabetes. Methods: The study used electrical stimulation with a direct voltage of 10 volts given a pulse voltage of 50-80 volts, a width of 50 µs, and the number of pulses of 65 per second. The research samples were Staphylococcus aureus (S. aureus) and Pseudo-monas aeruginosa (P. aeruginosa) bacteria that grew on beef and mice (Mus musculus) with diabetes. The treatment for S. aureus and P. aeruginosa bacteria was carried out using a pulse voltage of 50-80 volts for 5-15 min/day and repeated for 3 days. Meanwhile, treatment of mice wounds was carried out with a pulse voltage of 80 volts for 15 min/day and repeated for 7 days. Results: The results showed that treatment with a pulse voltage of 50-80 volts and a treatment time of 5-15 min significantly reduced the number of S. aureus and P. aeruginosa bacteria in beef (p£0.05). Treatment with a pulse voltage of 80 volts for 15 min made beef free from bacteria. Meanwhile, treatment with a pulse voltage of 80 volts for 15 min per day for seven days resulted in the wound state of three mice in the maturation phase and two mice in the proliferation phase on day 8 with an average wound area of 0.108 cm 2. Conclusion: The treatment with a pulse voltage of 80 volts for 15 min made the beef sterile, the mice wounds healed quickly, and the mice not stressed. The higher the blood glucose level, the slower the wound healing process.  

Wireless Electrical Stimulation

2013 ◽  
Vol 12 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Ourania Castana ◽  
Aekaterini Dimitrouli ◽  
Theodoros Argyrakos ◽  
Emilia Theodorakopoulou ◽  
Nektarios Stampolidis ◽  
...  
Keyword(s):  
Wound Healing ◽  
Electrical Stimulation ◽  
Beneficial Effect ◽  
Lower Limb ◽  
Therapeutic Option ◽  
Healing Process ◽  
Wound Healing Process ◽  
Effective Therapeutic Option ◽  
Chronic Ulcers ◽  
Stimulation Technique

High-voltage electrical stimulation has been long proposed as a method of accelerating the wound healing process. Its beneficial effect has been successfully evaluated in the treatment of a number of chronic ulcers and burns. We present here the implementation of a new wireless electrical stimulation technique for the treatment of a complicated chronic ulcer of the lower limb. The device is transferring charges to the wound, without any contact with it, creating a microcurrent that is able to generate the current of injury. The results suggest that this easy-to-use method is an effective therapeutic option for chronic ulcers.

scholarly journals Hydrogels for management of chronic wound healing

2021 ◽  
Vol 5 (3) ◽  
pp. 095-104
Author(s):  
IM Cardoso-Daodu ◽  
CP Azubuike ◽  
MO Ilomuanya
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Healing Process ◽  
Three Dimensional ◽  
Scar Tissue ◽  
The Body ◽  
Polymer Chains ◽  
Wound Healing Process ◽  
Dimensional Network ◽  
Healing Wound

Chronic wounds occur when one wound healing process or a sequence of wound healing events are affected resulting in slow healing of the wound thereby placing the patient in deep pain. Various diseases and conditions can delay the process of wound healing. Wound healing can be classified into four main stages: hemostasis, inflammation, remodeling, and scar tissue formation with each phase overlapping one another. The skin is the largest organ in the body. It protects the entire external surface of the human body and is the primary site of interaction with the outside environment. There is therefore a need to fabricate an ideal dressing through scientific research and investigations. Hydrogels are a three-dimensional network of hydrophilic polymers that can swell in water and absorb copious amounts of water while maintaining their structure because of their chemical or physical crosslinking of individual polymer chains. A hydrogel must be composed of at least 10% water. Hydrogels possess the flexibility and water percentage which is remarkably like tissues. They are biocompatible and biodegradable which makes them ideal for dermal wound healing.

scholarly journals Effectiveness of Aqueous Extract of Marine Baitworm Marphysa moribidii Idris, Hutchings and Arshad, 2014 (Annelida, Polychaeta), on Acute Wound Healing Using Sprague Dawley Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-15 ◽  
Author(s):  
Hannah Syahirah Rapi ◽  
Nor ‘Awatif Che Soh ◽  
Nurul Shahirah Mohd Azam ◽  
M. Maulidiani ◽  
Suvik Assaw ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Skin Irritation ◽  
Wound Healing Process ◽  
Histopathological Analysis ◽  
Pharmaceutical Drugs ◽  
Sprague Dawley ◽  
Normal Wound Healing ◽  
Wound Model ◽  
Ability To Regenerate

Wound healing is a well-coordinated process that restores skin integrity upon injury. However, some wound treatment poses harmful effects on the skin, which delay the normal wound healing process. Marphysa moribidii, a marine baitworm or polychaete, represents unique ability to regenerate posterior segment after injury, which may be beneficial in the wound healing treatment. The effectiveness of the polychaete as wound healing treatment was discovered through skin irritation, microbial testing, animal wound model, and chemical identifications. Three polychaete extracts (PE) emulsifying ointment (0.1%, 0.5%, and 1.0%) were topically applied to the full thickness wound model once daily for 14 days. Interestingly, PE 1.0% revealed the most rapid wound healing effects as compared to other treatments, including gamat (sea cucumber) oil (15% w/v) and acriflavine (0.1% w/v). Histopathological analysis using Masson’s trichrome staining further confirms that PE treated wound exhibited minimal scar, high collagen deposition, and the emergence of neovascularisation. The extract also displayed a minimum inhibitory concentration (MIC) of 0.4 g/ml against Escherichia coli and absence of skin irritation, infectious bacteria, and heavy metals from the extract. Moreover, chemical compounds such as alkaloid, flavonoid, amino acids, and organic acid were detected in M. moribidii extracts, which could contribute to wound healing activity. In conclusion, this study further justifies the beneficial use of polychaete in treating wound healing and could be developed as a novel bioactive agent in nutraceuticals and pharmaceutical drugs.

scholarly journals EVALUATION OF WOUND HEALING ACTIVITY OF POLYHERBAL FORMULATION

2017 ◽  
Vol 9 (6) ◽  
pp. 12
Author(s):  
Madhuri A. Theng ◽  
G. R. Sitaphale ◽  
K. R. Biyani
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Soxhlet Extraction ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Butea Monosperma ◽  
Polyherbal Formulation ◽  
Wound Model ◽  
Acacia Arabica ◽  
Wound Healing Activity

Objective: The present study describes the anti-microbial acivity of Acacia arabica and Butea monosperma bark extract.Methods: For this purpose aqueous extract of bark were prepared by “Soxhlet extraction method”. The experimentally induced burn wound model in rats by “Excision method”.Results: As a result of this study it was found that the extract of bark generally revealed antimicrobial and wound healing activity.Conclusion: The result of the study suggest that the Acacia arabica and Butea monosperma bark of polyherbal gel effective in accelerating wound healing process.

Upregulating mTOR/ERK signaling with leonurine for promoting angiogenesis and tissue regeneration in a full-thickness cutaneous wound model

2018 ◽  
Vol 9 (4) ◽  
pp. 2374-2385 ◽  
Author(s):  
Chenggui Wang ◽  
Zengjie Zhang ◽  
Tianzhen Xu ◽  
Yiting Lou ◽  
Qingqing Wang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Healing Process ◽  
Erk Signaling ◽  
Wound Healing Process ◽  
Erk Pathway ◽  
Full Thickness ◽  
Cutaneous Wound ◽  
Wound Model

LN promoted the angiogenesis of endothelial cells by activating the mTOR/ERK pathway, and efficiently enhanced the wound-healing processin vivo.

scholarly journals THE POTENTIAL ROLE OF GENUS TINOSPORA IN WOUND HEALING: A REVIEW

2021 ◽  
pp. 21-29
Author(s):  
MICHELE FERNANDEZ ◽  
NAVEEN KUMAR SHIVASHEKAREGOWDA ◽  
YOW HUI YIN
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Future Research ◽  
Wound Healing Process ◽  
Digestive Disorder ◽  
Healing Agent ◽  
Phytochemical Constituents ◽  
Underlying Mechanisms ◽  
Ethnomedicinal Uses

Genus Tinospora has been used in traditional medicine throughout various parts of Asia, particularly in India, China, Thailand, Malaysia and Cambodia. Its ethnomedicinal uses include treatment for fever, cold, oral ulcer, headaches, diarrhoea, diabetes, digestive disorder and rheumatoid arthritis. Some of its ethnomedicinal uses have been justified via scientific studies, include antidiabetic, antioxidant, anti-inflammatory and antimicrobial effects. Recently, several studies have been initiated to investigate the role of Tinospora species in wound healing. This comprehensive review used updated information obtained from systematic literature search using PubMed, Scopus, Science Direct and Google Scholar, to assess the wound healing properties of eleven major Tinospora species namely, T. cordifolia, T. crispa, T. sagittata, T. sinensis, T. bakis, T. craveniana, T. tuberculata, T. capillipes, T. rumphii, T. smilacina, and T. baenzigeri. The information obtained revealed that only T. cordifolia and T. crispa have been reported on its wound-healing abilities. Studies demonstrated that T. cordifolia is associated with early re-epithelisation, enhanced tensile strength, increased granulation tissues and increased collagen contents. Whereas T. crispa is reported with wound healing activity through the elevated antioxidant enzymes and inflammation phase. Nevertheless, there is limited evidence on phytochemical constituents from plant extracts that contribute to the wound healing process and their underlying mechanisms. This review deduced the potential gaps for future research aiming to study the genus Tinospora as a potential wound healing agent.

scholarly journals Review on experiment-based two- and three-dimensional models for wound healing

2016 ◽  
Vol 6 (5) ◽  
pp. 20160038 ◽  
Author(s):  
Daphne Weihs ◽  
Amit Gefen ◽  
Fred J. Vermolen
Keyword(s):  
Wound Healing ◽  
Mathematical Modelling ◽  
Chronic Wounds ◽  
Healing Process ◽  
Three Dimensional ◽  
Wound Healing Process ◽  
Medical Systems ◽  
Ageing Society ◽  
Three Dimensional Models ◽  
Induced Changes

Traumatic and chronic wounds are a considerable medical challenge that affects many populations and their treatment is a monetary and time-consuming burden in an ageing society to the medical systems. Because wounds are very common and their treatment is so costly, approaches to reveal the responses of a specific wound type to different medical procedures and treatments could accelerate healing and reduce patient suffering. The effects of treatments can be forecast using mathematical modelling that has the predictive power to quantify the effects of induced changes to the wound-healing process. Wound healing involves a diverse and complex combination of biophysical and biomechanical processes. We review a wide variety of contemporary approaches of mathematical modelling of gap closure and wound-healing-related processes, such as angiogenesis. We provide examples of the understanding and insights that may be garnered using those models, and how those relate to experimental evidence. Mathematical modelling-based simulations can provide an important visualization tool that can be used for illustrational purposes for physicians, patients and researchers.

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