scholarly journals Simultaneous Determination of 8 Compounds in Gancao-Ganjiang-Tang by HPLC-DAD and Analysis of the Relations between Compatibility, Dosage, and Contents of Medicines

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Yanfang Yang ◽  
Guijun Zhang ◽  
Qiyu Sun ◽  
Liang Liu ◽  
Hui Peng ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Quality Evaluation ◽  
Glycyrrhizic Acid ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Clinical Effectiveness ◽  
Medicinal Herbs ◽  
Tcm Theory ◽  
Ancient Times

Gancao-Ganjiang-Tang (GGT) is a traditional Chinese medicine (TCM) prescription and is a representative prescription for recuperating depleted Yang in Treatise on Febrile Diseases. The TCM theory believes that the efficacy of medicinal herbs is decided by the multicompounds which consist of different kinds of chemical constituents with bioactivities, but not by a monomeric constituent. From ancient times until today, GGT have 5 different kinds of compatibilities that can be verified. In this study, a HPLC-DAD method was established for the simultaneous determination of 8 compounds including 6-gingerol, 8-gingerol, 6-shogaol, liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin, and glycyrrhizic acid in the five GGT. The total contents of the 8 compounds in GGT varied from 555.56 to 956.33 μg/mL. The effects showed that the dosage and compatibility of medicinal herbs have influenced the content of chemical compounds of TCM prescription while the content of chemical compounds has acted on clinical efficacy. Quality evaluation and active essence screening of TCM (including single herb and prescription) should be based on the TCM theory and clinical effectiveness. The method was proven to be suitable for quality control of GGT.

scholarly journals Simultaneous Determination of Nine Active Compounds of the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang and Analysis of the Relationship between Therapeutical Effect and Compatibility of Medicines

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Guangwei Zhu ◽  
Guijun Zhang ◽  
Meng Wang ◽  
Jingjuan Wang ◽  
Weixin Zeng ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Glycyrrhizic Acid ◽  
Detection Method ◽  
Chemical Compounds ◽  
Active Compounds ◽  
Column Temperature ◽  
The Relationship

A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.

Validasi dari Spektrofotometri UV-Vis dan Kandungan Total Flavonoid Ekstrak Etanol dari Akar Alang-Alang (Imperata cylindrica) dan Daun Pegagan (Centella asiatica) Anita Puspa Widiyana

2021 ◽  
Vol 3 (2) ◽  
pp. 126-136
Author(s):  
Anita Puspa Widiyana ◽  
Keyword(s):  
Chemical Constituents ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Imperata Cylindrica ◽  
Total Flavonoid ◽  
Analysis Method ◽  
Flavonoid Content ◽  
Validation Testing

Validation as a quality control for the content of chemical compounds from natural ingredients. One of the chemical constituents is the flavonoids which are found in the Imperatacylindrica roots and Centella asiatica leaves. This study aims to ensure the analysis method meets the requirements and determines the levels of flavonoids. The research stages included extraction, validation and determination of total flavonoid. Extraction was carried out by immersing dry simplicia in 96% ethanol solvent for 3x24 hours. The solvent is evaporated using a rotary evaporator until a thick extract is formed. Validation testing includes linearity, accuracy, precision, LOD and LOQ. Determinationof the total flavonoid was carried out by measuring the absorption at a maximum wavelength of 428.2 nm. The validation results includethe correlation coefficient (R) of 0.998, precision % RSD <2 %, %accuracy 99,53-97,98%, LOD 3.02ppm and LOQ 9.15 ppm. The total flavonoid of the ethanol extract of Imperata cylindrica roots was 36.39 ± 0.08 mg/g QE and Centella asiatica leaves was 102.10 ± 0.08 mg/g QE. The conclusion is that the method used met the validation requirements and the total flavonoid content of the ethanol extract of Centella asiatica leaves was higher than Imperata cylindrica roots

Quality Evaluation of Angelica sinensis by Simultaneous Determination of Ten Compounds Using LC-PDA

2009 ◽  
Vol 70 (3-4) ◽  
pp. 455-465 ◽  
Author(s):  
Jie-Li Lü ◽  
Jing Zhao ◽  
Jin-Ao Duan ◽  
Hui Yan ◽  
Yu-Ping Tang ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Quality Evaluation ◽  
Angelica Sinensis

scholarly journals Determination of Bitterness of Andrographis Herba Based on Electronic Tongue Technology and Discovery of the Key Compounds of Bitter Substances

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3362 ◽  
Author(s):  
Xiao Zhang ◽  
Hongwei Wu ◽  
Xiankuo Yu ◽  
Hanyan Luo ◽  
Yaqi Lu ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
High Performance ◽  
Quality Evaluation ◽  
Herbal Remedy ◽  
Three Dimensional ◽  
Electronic Tongue ◽  
Principal Component ◽  
Chemical Compositions ◽  
Tcm Theory

Andrographis Herba (AH), the dry aerial segments of Andrographis paniculata (Burm.f.) Nees, is a common herbal remedy with bitter properties in traditional Chinese medicine (TCM) theory. Although bitterness is one of the features representing Chinese medicine, it has not been implemented as an index to assess the quality and efficacy of TCM because of peoples’ subjectivity to taste. In this study, 30 batches of AH with different commercial classifications (leaves, stems, or mixtures of both) were collected. Bitterness of AH was quantified by electronic tongue technology. Meanwhile, chemical compositions were characterized through establishing high-performance liquid chromatography fingerprints. The result indicated that the radar curves of the bitterness from different AH commercial classifications displayed different taste fingerprint information. Based on six taste factors, a Principal Component Analysis (PCA) score three-dimensional (3D) plot exhibited a clear grouping trend (R2X, 0.912; Q2, 0.763) among the three different commercial classifications. Six compounds (Peaks 2, 3, 4, 6, 7, 8) with positive correlation to bitterness were discovered by a Spearman correlation analysis. Peaks 2, 6, 7, 8 were identified as andrographolide, neoandrographolide, 14-deoxyandrographolide, and dehydroandrographolide, respectively. The electronic tongue can be used to distinguish AH samples with different commercial classifications and for quality evaluation.

Sign in / Sign up

Export Citation Format

Share Document