scholarly journals Simultaneous Determination of Nine Active Compounds of the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang and Analysis of the Relationship between Therapeutical Effect and Compatibility of Medicines

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Guangwei Zhu ◽  
Guijun Zhang ◽  
Meng Wang ◽  
Jingjuan Wang ◽  
Weixin Zeng ◽  
...  
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Glycyrrhizic Acid ◽  
Detection Method ◽  
Chemical Compounds ◽  
Active Compounds ◽  
Column Temperature ◽  
The Relationship

A simple and sensitive HPLC-DAD detection method was established for the simultaneous determination of nine compounds including oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, glycyrrhizic acid, liquiritin, isoliquiritin, liquiritigenin, and isoliquiritigenin in the Traditional Chinese Medicinal Prescription Shaoyao-Gancao-Tang (SGT) and we analyze the relationship between therapeutical effect and compatibility of medicines by using an Agilent extend-C18 column at a flow rate of 1 mL/min. The column temperature was maintained at 30°C and the detection wavelength was set at 230 nm for oxypaeoniflorin, albiflorin, paeoniflorin, benzoylpaeoniflorin, and glycyrrhizic acid; 276 nm for liquiritin and liquiritigenin; 360 nm for isoliquiritin and isoliquiritigenin. The total contents of the nine compounds in SGT varied from 4.65 to 20.06 mg/mL. The results of this study showed that the content of chemical compounds of Traditional Chinese Medicinal Prescription is mainly influenced by the dosage and compatibility of medicines and the therapeutical effect of Traditional Chinese Medicinal prescription is mainly influenced by the dosage and compatibility of medicines. The method could be suitable for quality control of SGT with bioactive multicompounds.

Determination of Paeoniflorin and Paeonol in Houyinan Tablet by UPLC

2012 ◽  
Vol 581-582 ◽  
pp. 68-72
Author(s):  
Chu Qin Yu ◽  
Hua Qing Lin ◽  
Yue Han Hou ◽  
Zhong Feng Shi ◽  
Di Shi Lin
Keyword(s):  
Quality Control ◽  
Simultaneous Determination ◽  
Flow Rate ◽  
Mobile Phase ◽  
Gradient Elution ◽  
The Other ◽  
Column Temperature ◽  
Average Recovery ◽  
Injection Volume

In this study, our purpose was to establish a UPLC method for the simultaneous determination of Paeoniflorin and Paeonol in Houyinan Tablet. The separation was performed on Acquity BEH C18 column(2.1mm×100mm,1.7μm), the mobile phase was acetonitrile-water with gradient elution at a flow rate of 0.2 mL•min-1, the detection wavelength was 230nm, the column temperature was 30°Cand the injection volume was 2μL. Paeoniflorin and Paeonol reached effective separation with the other components in this chromatographic conditions. Paeoniflorin and Paeonol were linear within the range of 0.0406~0.4064μg(r=0.9999) and 0.0426~0.4256μg (r=0.9999), respectively. The average recovery was 99.82% and 100.6%. The results of method validation indicated that the method was simple,quick,accurate, specific and less solvent consumption. It can be used for the quality control of Houyinan Tablet.

Determination of Sodium Salvia Miltiorrhiza’s Optimal Analytical Conditions Detected by HPLC Method

2013 ◽  
Vol 446-447 ◽  
pp. 235-239
Author(s):  
Tao Wang
Keyword(s):  
Quality Control ◽  
Correlation Coefficient ◽  
Flow Rate ◽  
Salvia Miltiorrhiza ◽  
Hplc Method ◽  
Uv Detector ◽  
Experimental Conditions ◽  
Column Temperature ◽  
Extraction And Purification

The optimal analytical conditions of sodium salvia miltiorrhiza are detected by HPLC methods. SinoChrom ODS-BP C18 is selected as chromatographic column. The optimal experimental conditions under 280 nm wavelength UV detector include the column temperature: 28°C, ratio of methanol/water: 25/75 (v/v), and flow rate: 1.0ml/mol. The correlation coefficient is 0.9983, and RSD is 1.4%, when the linear range is 0.15~1.8μg/μL. In the experiment, the method is verified to be simple and reliable, and which can be used for the quality control in process of salvia miltiorrhiza extraction and purification.

scholarly journals Simultaneous Determination of 8 Compounds in Gancao-Ganjiang-Tang by HPLC-DAD and Analysis of the Relations between Compatibility, Dosage, and Contents of Medicines

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Yanfang Yang ◽  
Guijun Zhang ◽  
Qiyu Sun ◽  
Liang Liu ◽  
Hui Peng ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Quality Evaluation ◽  
Glycyrrhizic Acid ◽  
Chemical Constituents ◽  
Chemical Compounds ◽  
Clinical Effectiveness ◽  
Medicinal Herbs ◽  
Tcm Theory ◽  
Ancient Times

Gancao-Ganjiang-Tang (GGT) is a traditional Chinese medicine (TCM) prescription and is a representative prescription for recuperating depleted Yang in Treatise on Febrile Diseases. The TCM theory believes that the efficacy of medicinal herbs is decided by the multicompounds which consist of different kinds of chemical constituents with bioactivities, but not by a monomeric constituent. From ancient times until today, GGT have 5 different kinds of compatibilities that can be verified. In this study, a HPLC-DAD method was established for the simultaneous determination of 8 compounds including 6-gingerol, 8-gingerol, 6-shogaol, liquiritin, liquiritigenin, isoliquiritin, isoliquiritigenin, and glycyrrhizic acid in the five GGT. The total contents of the 8 compounds in GGT varied from 555.56 to 956.33 μg/mL. The effects showed that the dosage and compatibility of medicinal herbs have influenced the content of chemical compounds of TCM prescription while the content of chemical compounds has acted on clinical efficacy. Quality evaluation and active essence screening of TCM (including single herb and prescription) should be based on the TCM theory and clinical effectiveness. The method was proven to be suitable for quality control of GGT.

scholarly journals A Simple and Convenient Method for Simultaneous Determination ofSchizandrol A,Schizandrol B,Schisandrin A,γ-Schisandrin, andSchisandrin C

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Yu Zhou ◽  
Wu Ling Wei ◽  
Jiang Xing Hua ◽  
Qingsheng Fan
Keyword(s):  
Simultaneous Determination ◽  
Flow Rate ◽  
Convenient Method ◽  
Mobile Phase ◽  
Gradient Elution ◽  
Hplc Method ◽  
Schisandra Chinensis ◽  
Column Temperature

A simple, rapid, and specific HPLC method was established for simultaneous determination of five major lignans (Schizandrol A,Schizandrol B,Schisandrin A,γ-Schisandrin, andSchisandrin C) inSchisandra chinensis. The five lignans can be separated completely on Kromasil C18column (250 nm × 4.6 nm) and then detected at 254 nm using methanol (mobile phase A) and water (mobile phase B) with gradient elution as the mobile phase at 1.0 mL/min flow rate. The column temperature was 30°C. The method was validated in terms of linearity, precision, stability, repeatability, and recovery. Results showed that the method is accurate and reproducible.

Simultaneous Determination of Eight Potential Q-Markers in Zishen Tongguan Capsules Based on UHPLC-MS/MS

2020 ◽  
Vol 17 (1) ◽  
pp. 47-56
Author(s):  
Shun Liu ◽  
Xun Wang ◽  
Kaiping Zou ◽  
Wei Liu ◽  
Cunyu Li ◽  
...  
Keyword(s):  
Quality Control ◽  
Chinese Medicine ◽  
Simultaneous Determination ◽  
High Performance ◽  
Multiple Reaction Monitoring ◽  
Gradient Elution ◽  
Quality Markers ◽  
High Repeatability ◽  
Comprehensive Study

Background: Zishen Tongguan (ZSTG) capsules were prepared at the Affiliated Hospital of Nanjing University of Chinese Medicine and have been proven to be clinically effective for treating pyelonephritis and benign prostatic hyperplasia. However, the quality standards are not ideal; a comprehensive study of the “quality markers” (Q-markers), the chemicals inherent in traditional Chinese medicine and its preparations, has not been carried out. Experimental Methods: In this paper, a sensitive and specific ultra-high-performance liquid chromatographictandem mass spectrometry (UHPLC-MS/MS) method was developed for the simultaneous determination of eight potential Q-markers of ZSTG, including timosaponin A3, berberine, jatrorrhizine, phellodendrine, palmatine, mangiferin, neomangiferin, and timosaponin BII. A Kromasil 100-3.5 C18 column was used with a mobile phase of 0.2% formic acid with acetonitrile, and gradient elution at a flow rate of 0.2 mL/min was achieved in 13 minutes and used for separation. Detection was performed in positive/negative mode with multiple reaction monitoring (MRM). Results: The analytical method was validated in terms of the sensitivity, linearity, accuracy, precision, repeatability, stability and recovery. The method established here was successfully applied to study the potential Q-markers in 8 batches of commercial samples, which demonstrated its use in improving the quality control of ZSTG. Conclusion: The developed method had high repeatability and accuracy and was suitable for the simultaneous analysis of multiple Q-markers, which may provide a new basis for the comprehensive assessment and overall quality control of ZSTG.

Comparison Study on the Contents of Eight Flavonoids in three Different Processed Products of Scutellariae Radix using Ultra-high Performance Liquid Chromatography Coupled With Triple-Quadrupole Mass Spectrometry

2020 ◽  
Vol 16 (6) ◽  
pp. 690-697
Author(s):  
Yuedong Yang ◽  
Hao Zhi ◽  
Baofei Yan ◽  
Yi Tian ◽  
Jianping Shen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Triple Quadrupole ◽  
Column Temperature ◽  
Scutellariae Radix ◽  
Total Percentage

Background: The simultaneous determination of multiple components in a sample is an important factor in the quality control of traditional Chinese medicines and can give an indication of potential clinical applications. Introduction: A rapid and sensitive method has been introduced for the simultaneous quantitative analysis of eight bioactive flavonoid constituents from Scutellariae Radix using ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry. Methods: The separation was performed on a Waters Acquity UPLC C18 column (2.1 mm×100 mm, 1.7 μm), under optimized mass spectrometry conditions, with a flow rate of 0.3 mL/min. The column temperature was maintained at 35°C and the injection volume was 3 μL. Results: The method showed a good linear relationship of each component; all R2 values were above 0.9990 in the experiment. The RSDs of the precision test, repeatability test, stability test and recovery test were all not more than 2.86 %. We found that the total percentage amounts of the eight flavonoids were 22.19%, 18.63% and 10.86% in Raw Scutellariae Radix (RSR), Wine Scutellaria Radix (WSR) and Scutellaria Radix Charcoal (SRC) respectively. Conclusion: The method was successfully applied to the simultaneous determination of the eight bioactive flavonoids of Raw Scutellariae Radix, Wine Scutellaria Radix and Scutellaria Radix Charcoal.

scholarly journals Microchip electrophoresis with amperometric detection method for profiling cellular nitrosative stress markers

The Analyst ◽  
2014 ◽  
Vol 139 (13) ◽  
pp. 3265-3273 ◽  
Author(s):  
Dulan B. Gunasekara ◽  
Joseph M. Siegel ◽  
Giuseppe Caruso ◽  
Matthew K. Hulvey ◽  
Susan M. Lunte
Keyword(s):  
Simultaneous Determination ◽  
Nitrosative Stress ◽  
Detection Method ◽  
Amperometric Detection ◽  
Microchip Electrophoresis ◽  
Macrophage Cells ◽  
Stress Markers ◽  
Electrophoresis Method ◽  
Endogenous Antioxidants

Microchip electrophoresis method for the simultaneous determination of intracellular nitrosative stress markers and endogenous antioxidants in native and stimulated macrophage cells.

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