scholarly journals Role of Protein Kinase C and Nox2-Derived Reactive Oxygen Species Formation in the Activation and Maturation of Dendritic Cells by Phorbol Ester and Lipopolysaccharide

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Judith Stein ◽  
Sebastian Steven ◽  
Matthias Bros ◽  
Stephan Sudowe ◽  
Michael Hausding ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dendritic Cells ◽  
Protein Kinase C ◽  
T Cell ◽  
Oxygen Species ◽  
Kinase C ◽  
Stimulatory Capacity ◽  
Ros Formation ◽  
Cell Stimulatory Capacity

Aims. Activation/maturation of dendritic cells (DCs) plays a central role in adaptive immune responses by antigen processing and (cross-) activation of T cells. There is ongoing discussion on the role of reactive oxygen species (ROS) in these processes and with the present study we investigated this enigmatic pathway.Methods and Results. DCs were cultured from precursors in the bone marrow of mice (BM-DCs) and analyzed for ROS formation, maturation, and T cell stimulatory capacity upon stimulation with phorbol ester (PDBu) and lipopolysaccharide (LPS). LPS stimulation of BM-DCs caused maturation with moderate intracellular ROS formation, whereas PDBu treatment resulted in maturation with significant ROS formation. The NADPH oxidase inhibitors apocynin/VAS2870 and genetic gp91phox deletion both decreased the ROS signal in PDBu-stimulated BM-DCs without affecting maturation and T cell stimulatory capacity of BM-DCs. In contrast, the protein kinase C inhibitors chelerythrine/Gö6983 decreased PDBu-stimulated ROS formation in BM-DCs as well as maturation.Conclusion. Obviously Nox2-dependent ROS formation in BM-DCs is not always required for their maturation or T cell stimulatory potential. PDBu/LPS-triggered BM-DC maturation rather relies on phosphorylation cascades. Our results question the role of oxidative stress as an essential “danger signal” for BM-DC activation, although we cannot exclude contribution by other ROS sources.

The role of nitric oxide, reactive oxygen species, and protein kinase C in oxytocin-induced cardioprotection in ischemic rat heart

Peptides ◽  
2012 ◽  
Vol 37 (2) ◽  
pp. 314-319 ◽  
Author(s):  
Mahdieh Faghihi ◽  
Ali Mohammad Alizadeh ◽  
Vahid Khori ◽  
Mostafa Latifpour ◽  
Saeed Khodayari
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Heart ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Kinase C

Reactive Oxygen Species Precede the ε Isoform of Protein Kinase C in the Anesthetic Preconditioning Signaling Cascade

2003 ◽  
Vol 99 (2) ◽  
pp. 421-428 ◽  
Author(s):  
Enis Novalija ◽  
Leo G. Kevin ◽  
Amadou K.S. Camara ◽  
Zeljko J. Bosnjak ◽  
John P. Kampine ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinase C ◽  
Signaling Cascade ◽  
Oxygen Species ◽  
Anesthetic Preconditioning ◽  
Pkc Delta ◽  
Kinase C ◽  
Pkc Epsilon ◽  
Ros Formation ◽  
Pkc Activation

Background Protein kinase C (PKC) and reactive oxygen species (ROS) are known to have a role in anesthetic preconditioning (APC). Cardiac preconditioning by triggers other than volatile anesthetics, such as opioids or brief ischemia, is known to be isoform selective, but the isoform required for APC is not known. The authors aimed to identify the PKC isoform that is involved in APC and to elucidate the relative positions of PKC activation and ROS formation in the APC signaling cascade. Methods Isolated guinea pig hearts were subjected to 30 min of ischemia and 120 min of reperfusion. Before ischemia, hearts were either untreated or treated with sevoflurane (APC) in the absence or presence of the nonspecific PKC inhibitor chelerythrine, the PKC-delta inhibitor PP101, or the PKC-epsilon inhibitor PP149. Spectrofluorometry and the fluorescent probes dihydroethidium were used to measure intracellular ROS, and effluent dityrosine as used to measure extracellular ROS release. Results Previous sevoflurane exposure protected the heart against ischemia-reperfusion injury, as previously described. Chelerythrine or PP149 abolished protection, but PP101 did not. ROS formation was observed during sevoflurane exposure and was not altered by any of the PKC inhibitors. Conclusions APC is mediated by PKC-epsilon but not by PKC-delta. Furthermore, PKC activation probably occurs downstream of ROS generation in the APC signaling cascade.

scholarly journals Role of p90 Ribosomal S6 Kinase (p90RSK) in Reactive Oxygen Species and Protein Kinase C β (PKC-β)-mediated Cardiac Troponin I Phosphorylation

2005 ◽  
Vol 280 (25) ◽  
pp. 24135-24142 ◽  
Author(s):  
Seigo Itoh ◽  
Bo Ding ◽  
Christopher P. Bains ◽  
Nadan Wang ◽  
Yasuchika Takeishi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Protein Kinase C ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Oxygen Species ◽  
S6 Kinase ◽  
Kinase C ◽  
Pkc Β ◽  
Ribosomal S6 Kinase
Sign in / Sign up

Export Citation Format

Share Document