scholarly journals Gd3+-Asparagine-Anionic Linear Globular Dendrimer Second-Generation G2 Complexes: Novel Nanobiohybrid Theranostics

2017 ◽  
Vol 2017 ◽  
pp. 1-19 ◽  
Author(s):  
Nasim Hashempour Alamdari ◽  
Mahmood Alaei-Beirami ◽  
Seyed Ataollah Sadat Shandiz ◽  
Hadi Hejazinia ◽  
Rahimeh Rasouli ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Relaxation Times ◽  
Cost Effective ◽  
Water Soluble ◽  
Brain Targeting ◽  
Tumor Growth Inhibition ◽  
Effective Agent ◽  
Water Soluble Polymer ◽  
Mr Relaxation

Designing a unique theranostic biocompatible, biodegradable, and cost-effective agent which is easy to be synthesized as a biohybrid material was the aim of this study. In this matter, asparagine attached to anionic linear globular dendrimer G2 (as a biocompatible, biodegradable, and cost-effective agent which is negatively charged nanosized and water soluble polymer that outweighs other traditionally used dendrimers) and finally contrast agent (Gd3+) was loaded (which made complexes) in synthesized asparagine-dendrimer. Observations revealed that, in addition to successful colon cancer and brain targeting, Gd3+-dendrimer-asparagine, the proposed theranostic agent, could increase T1 MR relaxation times, decrease T2 MR relaxation times significantly, and improve contrast of image as well as illustrating good cellular uptake based on florescent microscopy/flow cytometry and ICP-mass data. In addition to that, it increased tumor growth inhibition percentage (TGI%) significantly compared to FDA approved contrast agent, Magnevist. Totally, Gd3+-anionic linear globular dendrimer G2-asparagine could be introduced to the cancer imaging/therapy (theranostics) protocols after in vivo MR and fluorescent analysis and passing clinical trials. Hence, this nanotheranostic agent would be a promising candidate for brain drug delivery and imaging in the future.

Abstract 403: Tracking the Migration of Porcine Mesenchymal Stem Cells with the MRI Contrast Agent Ferex in an AAA Model

2012 ◽  
Vol 32 (suppl_1) ◽  
Author(s):  
Rami Tadros ◽  
Bhakti Rawal ◽  
Karen Briley-Saebo ◽  
David O’Connor ◽  
Dan Han ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Contrast Agent ◽  
Lentiviral Vector ◽  
Relaxation Times ◽  
Mri Contrast Agent ◽  
Signal Loss ◽  
Post Transplantation ◽  
Mri Contrast

Introduction: Mesenchymal stem cells (MSC) are being investigated in porcine abdominal aortic aneurysm (PAAA) models for their repair potential. This study uses MSCs labeled with the MRI contrast agent Ferex to non-invasively evaluate MSC migration in-vivo. Methods: MSCs from 6 pigs were isolated from bone marrow via Ficoll Paque separation and expanded in culture. Using a Lentiviral vector, MSC from all 6 pigs were transfected with green florescent protein (GFP). MSCs from 4 of these pigs were also labeled with 200μg/ml Ferex using Poly-L-Lysine and then analyzed for Ferex uptake and viability. Preservation of the MSC phenotype was confirmed using flow cytometry by detecting positive CD90 and negative CD45 and CD117. Transmission electron microscopy established that Ferex localized to lysosomes. MSCs were then injected into the adventitia of the PAAA. In-vivo MRI was performed using multiple echo gradient echo sequences. Effective transverse relaxation times (T2* values) were calculated on a pixel-by-pixel basis as a function of time post cell transplantation. Results: Ferex labeled MSCs were visible post transplantation at 4, 11, 15 and 21 days using MRI. The MRI signal void (decreased T2* values) correlated with the presence of Ferex within the PAAA. This signal loss progressively expanded circumferentially at each study interval representing cellular movement. MSC migration and localization were confirmed with GFP visualization on fluorescence microscopy and immunohistochemistry. In-vivo MRI signals also correlate with iron deposition on Perl’s stain. Conclusion: Ferex can be used as an in-vivo tracking agent of MSCs in PAAA models.

Synthesis of Nanostructured Magnetic Mixed-Oxide Ferrite Powders by Using A Novel Chemical Method

2002 ◽  
Vol 720 ◽  
Author(s):  
N N Ghosh
Keyword(s):  
Particle Size ◽  
Mixed Oxide ◽  
Chemical Method ◽  
Average Particle Size ◽  
Cost Effective ◽  
Water Soluble ◽  
Average Particle ◽  
Chemical Route ◽  
Water Soluble Polymer ◽  
Different Temperatures

AbstractIn the present investigation, an attempt has been made to establish a new chemical route for synthesis of the nanostructured mixed oxide ferrite powders. By using this chemical method a variety of ferrite powders having spinel structure and doped with Co, Ni, Mn, Zn etc has been prepared. In this method nitrate salts of the different metals were used as starting materials. The aqueous solutions of the metal nitrates were mixed according to the molar ration of the compositions. Then the mixtures were mixed with an aqueous solution of water soluble polymer (polyvinyl alcohol). This mixture after drying yield fluffy brown powders. These powders were then calcined at different temperatures ranging from 400 °C to 700 °C. Nanostructured powders were obtained from the thermal decomposition of the brown powders. The powders, prepared by calcinations at different temperatures, were characterized by using X-Ray diffraction analysis, IR spectroscopy, TGA/DTA, and TEM. It was observed that the average particle size of the powders are in nanometer scale with a narrow size distribution. The average particle size of the powders was increased with the increase of calcinations temperature.This chemical method has proved to provide a convenient process for the preparation of nanostructured ceramic powders at comparatively low temperatures and offers the potential of being a simple and cost-effective route.

scholarly journals High-throughput preparation of radioprotective polymers via Hantzsch’s reaction for in vivo X-ray damage determination

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Guoqiang Liu ◽  
Yuan Zeng ◽  
Tong Lv ◽  
Tengfei Mao ◽  
Yen Wei ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
High Throughput ◽  
Water Soluble ◽  
Mechanistic Study ◽  
Initial Attempt ◽  
Water Soluble Polymer ◽  
X Ray ◽  
In Vivo Experiments ◽  
Acute Injuries

AbstractRadioprotectors for acute injuries caused by large doses of ionizing radiation are vital to national security, public health and future development of humankind. Here, we develop a strategy to explore safe and efficient radioprotectors by combining Hantzsch’s reaction, high-throughput methods and polymer chemistry. A water-soluble polymer with low-cytotoxicity and an excellent anti-radiation capability has been achieved. In in vivo experiments, this polymer is even better than amifostine, which is the only approved radioprotector for clinical applications, in effectively protecting zebrafish embryos from fatally large doses of ionizing radiation (80 Gy X-ray). A mechanistic study also reveals that the radioprotective ability of this polymer originates from its ability to efficiently prevent DNA damage due to high doses of radiation. This is an initial attempt to explore polymer radioprotectors via a multi-component reaction. It allows exploiting functional polymers and provides the underlying insights to guide the design of radioprotective polymers.

scholarly journals Changes in MR relaxation times of the meniscal body with loading: an in vivo pilot study in knee osteoarthritis

2013 ◽  
Vol 21 ◽  
pp. S213
Author(s):  
K. Subburaj ◽  
R.B. Souza ◽  
B.T. Wyman ◽  
X. Li ◽  
T.M. Link ◽  
...  
Keyword(s):  
Pilot Study ◽  
Knee Osteoarthritis ◽  
Relaxation Times ◽  
Mr Relaxation

Rapid relaxation times measurements by MRI: an in vivo application to contrast agent modeling for muscle fiber types characterization

2000 ◽  
Vol 18 (10) ◽  
pp. 1221-1233 ◽  
Author(s):  
Veronique Dedieu ◽  
Pierre Fau ◽  
Philippe Otal ◽  
Jean-Pierre Renou ◽  
Valerie Emerit ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Muscle Fiber ◽  
Relaxation Times ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Agent Modeling

scholarly journals Changes in MR relaxation times of the meniscus with acute loading: An in vivo pilot study in knee osteoarthritis

2013 ◽  
Vol 41 (2) ◽  
pp. 536-543 ◽  
Author(s):  
Karupppasamy Subburaj ◽  
Richard B. Souza ◽  
Bradley T. Wyman ◽  
Marie-Pierre Hellio Le Graverand-Gastineau ◽  
Xiaojuan Li ◽  
...  
Keyword(s):  
Pilot Study ◽  
Knee Osteoarthritis ◽  
Relaxation Times ◽  
Mr Relaxation

scholarly journals Zonal differences in meniscus MR relaxation times in response to in vivo static loading in knee osteoarthritis

2015 ◽  
Vol 34 (2) ◽  
pp. 249-261 ◽  
Author(s):  
Nathaniel E. Calixto ◽  
Deepak Kumar ◽  
Karupppasamy Subburaj ◽  
Justin Singh ◽  
Joseph Schooler ◽  
...  
Keyword(s):  
Knee Osteoarthritis ◽  
Static Loading ◽  
Relaxation Times ◽  
Mr Relaxation

A monomeric water-soluble NIR-absorbing porphyrin derivative as in vivo photoacoustic tomography contrast agent

2017 ◽  
Author(s):  
Mohsen Erfanzadeh ◽  
Michael Luciano ◽  
Feifei Zhou ◽  
Christian Brückner ◽  
Quing Zhu
Keyword(s):  
Contrast Agent ◽  
Water Soluble ◽  
Photoacoustic Tomography ◽  
Porphyrin Derivative
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