scholarly journals Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Nagarajan Selvamurugan ◽  
Zhiming He ◽  
Daniel Rifkin ◽  
Branka Dabovic ◽  
Nicola C. Partridge
Keyword(s):  
Bone Marrow ◽  
Signaling Pathway ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Cell Structure ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Pulsed Electromagnetic ◽  
Stimulation Of

Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in nonunions and increase lumbar spinal fusion rates. However, the molecular mechanisms by which PEMF stimulates differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts are not well understood. In this study the PEMF effects on hBMSCs were studied by microarray analysis. PEMF stimulation of hBMSCs’ cell numbers mainly affected genes of cell cycle regulation, cell structure, and growth receptors or kinase pathways. In the differentiation and mineralization stages, PEMF regulated preosteoblast gene expression and notably, the transforming growth factor-beta (TGF-β) signaling pathway and microRNA 21 (miR21) were most highly regulated. PEMF stimulated activation of Smad2 and miR21-5p expression in differentiated osteoblasts, and TGF-β signaling was essential for PEMF stimulation of alkaline phosphatase mRNA expression. Smad7, an antagonist of the TGF-β signaling pathway, was found to be miR21-5p’s putative target gene and PEMF caused a decrease in Smad7 expression. Expression of Runx2 was increased by PEMF treatment and the miR21-5p inhibitor prevented the PEMF stimulation of Runx2 expression in differentiating cells. Thus, PEMF could mediate its effects on bone metabolism by activation of the TGF-β signaling pathway and stimulation of expression of miR21-5p in hBMSCs.

scholarly journals Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinwu Bai ◽  
Jianxiang Xu ◽  
Kai Hang ◽  
Zhihui Kuang ◽  
Li Ying ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Glycyrrhizic Acid ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Histological Evaluation

Glycyrrhizic acid (GA) is a major triterpene glycoside isolated from liquorice root that has been shown to inhibit osteoclastogenesis. However, there have been no reports regarding the effect of GA on osteogenic differentiation. Therefore, this study was performed to explore the effects and mechanism of action of GA on osteogenesis. A CCK-8 array was used to assess cell viability. The osteogenic capability was investigated by real-time quantitative PCR, western blotting and immunofluorescence analyses. ALP staining and ARS were used to evaluate ALP activity and mineralization, respectively. GA-GelMA hydrogels were designed to verify the therapeutic effects of GA in vivo by radiographic analysis and histological evaluation. Our results show that GA had no significant influence on the viability or proliferation of human bone marrow stromal cells (hBMSCs). GA promoted osteogenic differentiation and enhanced calcium deposition. Furthermore, ratio of active β-catenin and total β-catenin protein increased after treatment with GA. Wnt/catenin signaling inhibitor partially attenuated the effects of GA on osteogenic differentiation. In a mouse femoral fracture model, GA-GelMA hydrogels accelerated bone healing. Our results show that GA promotes the osteogenic differentiation of hBMSCs by modulating the Wnt/β-catenin signaling pathway. GA-GelMA hydrogels promoted bone fracture healing. GA has potential as a cost-effective treatment of bone defects.

Therapeutic Impact of Human Bone Marrow Stromal Cells Expanded by Animal Serum–Free Medium for Cerebral Infarct in Rats

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1733-1742 ◽  
Author(s):  
Taku Sugiyama ◽  
Satoshi Kuroda ◽  
Yukari Takeda ◽  
Mitsufumi Nishio ◽  
Masaki Ito ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Therapeutic Impact

scholarly journals Leptin Induces Apoptosis via ERK/cPLA2/CytochromecPathway in Human Bone Marrow Stromal Cells

2003 ◽  
Vol 278 (24) ◽  
pp. 21920-21929 ◽  
Author(s):  
Ghi Su Kim ◽  
Jeong Soo Hong ◽  
Seung Wook Kim ◽  
Jung-Min Koh ◽  
Chung Sun An ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals Directed Differentiation of Human Bone Marrow Stromal Cells to Fate-Committed Schwann Cells

2017 ◽  
Vol 9 (4) ◽  
pp. 1097-1108 ◽  
Author(s):  
Sa Cai ◽  
Yat-Ping Tsui ◽  
Kin-Wai Tam ◽  
Graham Ka-Hon Shea ◽  
Richard Shek-Kwan Chang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Schwann Cells ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Directed Differentiation
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