Stimulation of human bone marrow stromal cells using growth factor encapsulated calcium carbonate porous microspheres

2004 ◽  
Vol 14 (14) ◽  
pp. 2206 ◽  
Author(s):  
David Green ◽  
Dominic Walsh ◽  
Xeubin Yang ◽  
Stephen Mann ◽  
Richard O. C. Oreffo
Keyword(s):  
Bone Marrow ◽  
Calcium Carbonate ◽  
Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Porous Microspheres ◽  
Stimulation Of

Growth factor induced fibroblast differentiation from human bone marrow stromal cells in vitro

2005 ◽  
Vol 23 (1) ◽  
pp. 164-174 ◽  
Author(s):  
Jodie E. Moreau ◽  
Jingsong Chen ◽  
Diah S. Bramono ◽  
Vladimir Volloch ◽  
Herman Chernoff ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

Insulin-Like Growth Factor 1 Induced Expression of Vascular Endothelial Growth Factor, Which Is Dependent on MAP Kinase and Phosphatidylinositol 3-Kinase Signaling in Human Bone Marrow Stromal Cells.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4305-4305
Author(s):  
Gexiu Liu ◽  
Guangxiao Tang
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Brain Injuries ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Vegf Mrna ◽  
Vegf Protein

Abstract INTRODUCTION: Administration of bone marrow stromal cells after traumatic brain injury provides functional benefit. Its protective mechanisms include neurogenesis and angiogenesis. Different traumatic brain injuries have shown that vascular endothelial growth factor (VEGF) induced neuroprotection, neurogenesis, and angiogenesis. Insulin-like growth factor 1 (IGF-1) is a 7.5-kDa peptide with structural homology to proinsulin. In the adult organism, the liver is the major source of IGF-1. Although IGF-1 is very low in the normal adult rat brain, a number of studies have shown that IGF-1 is strongly induced in the CNS, and exerted its mitogenic and trophic effects on a variety of cell-types after different traumatic brain injuries, suggesting its repair roles after brain damage. We tested the hypothesis that IGF-1 induces expression of VEGF in human bone marrow stromal cells, and its signaling pathway. METHODS: Human bone marrow adherent cells were cultured, and were passaged in DMEM/F12 containing 10% FBS. The fifth passage cells were identified as stromal cells. Subconfluent cells were used, and were washed twice in PBS, then cultured in serum-free DMEM/F12. After overnight incubation, cells were exposed to IGF-1 (100 ng/ml) in the presence of no kinase inhibitor or a 1 h pretreatment with 50 μM PD98059, or 200 nM wortmannin. Cells were harvested after 2 h for analysis of active phosphorylated kinases (Akt and MAPK) or after 24 h for analysis of VEGF protein and mRNA. VEGF mRNA was detected by semi-quantitative RT-PCR, and VEGF protein by ELISA, and kinases by Western blot. RESULTS: 100 ng/ml IGF-1 increased significantly both VEGF mRNA and VEGF protein, which were very low level in control cells. 24 hours after treatment, ratio of RT-PCR product between VEGF and β-actin reach to (38.93±6.73)% from (18.61±4.25)% of control cells (P<0.01). VEGF protein increased to (123.45±20.86)pg/ml from (46.97±8.91)pg/ml (P<0.01). Moreover, IGF-1 induced active phosphorylated kinases (Akt and MAPK). And PD98059 or wortmannin inhibited effects of IGF-1. CONCLUSION: IGF-1 enhanced expression of VEGF mRNA and VEGF protein in human bone marrow stromal cells, which is dependent on MAP kinase and phosphatidylinositol 3-kinase signaling. These results suggest that VEGF be involved in therapeutic effects of bone marrow stromal cells in ischemic disorders, including stroke.

scholarly journals Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

2017 ◽  
Vol 2017 ◽  
pp. 1-17 ◽  
Author(s):  
Nagarajan Selvamurugan ◽  
Zhiming He ◽  
Daniel Rifkin ◽  
Branka Dabovic ◽  
Nicola C. Partridge
Keyword(s):  
Bone Marrow ◽  
Signaling Pathway ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Cell Structure ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Pulsed Electromagnetic ◽  
Stimulation Of

Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in nonunions and increase lumbar spinal fusion rates. However, the molecular mechanisms by which PEMF stimulates differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts are not well understood. In this study the PEMF effects on hBMSCs were studied by microarray analysis. PEMF stimulation of hBMSCs’ cell numbers mainly affected genes of cell cycle regulation, cell structure, and growth receptors or kinase pathways. In the differentiation and mineralization stages, PEMF regulated preosteoblast gene expression and notably, the transforming growth factor-beta (TGF-β) signaling pathway and microRNA 21 (miR21) were most highly regulated. PEMF stimulated activation of Smad2 and miR21-5p expression in differentiated osteoblasts, and TGF-β signaling was essential for PEMF stimulation of alkaline phosphatase mRNA expression. Smad7, an antagonist of the TGF-β signaling pathway, was found to be miR21-5p’s putative target gene and PEMF caused a decrease in Smad7 expression. Expression of Runx2 was increased by PEMF treatment and the miR21-5p inhibitor prevented the PEMF stimulation of Runx2 expression in differentiating cells. Thus, PEMF could mediate its effects on bone metabolism by activation of the TGF-β signaling pathway and stimulation of expression of miR21-5p in hBMSCs.

scholarly journals Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells

2017 ◽  
Vol 24 ◽  
pp. 69-76 ◽  
Author(s):  
Sigrid Müller-Deubert ◽  
Lothar Seefried ◽  
Melanie Krug ◽  
Franz Jakob ◽  
Regina Ebert
Keyword(s):  
Bone Marrow ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Epidermal Growth

Therapeutic Impact of Human Bone Marrow Stromal Cells Expanded by Animal Serum–Free Medium for Cerebral Infarct in Rats

Neurosurgery ◽  
2011 ◽  
Vol 68 (6) ◽  
pp. 1733-1742 ◽  
Author(s):  
Taku Sugiyama ◽  
Satoshi Kuroda ◽  
Yukari Takeda ◽  
Mitsufumi Nishio ◽  
Masaki Ito ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Therapeutic Impact
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