scholarly journals The Neurovascular Properties of Dental Stem Cells and Their Importance in Dental Tissue Engineering

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Jessica Ratajczak ◽  
Annelies Bronckaers ◽  
Yörg Dillen ◽  
Pascal Gervois ◽  
Tim Vangansewinkel ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tissue Growth ◽  
Deciduous Teeth ◽  
Human Tooth ◽  
Dental Stem Cells ◽  
Dental Tissue ◽  
Periodontal Ligament Stem Cells ◽  
Essential Components ◽  
Dental Tissue Engineering

Within the field of tissue engineering, natural tissues are reconstructed by combining growth factors, stem cells, and different biomaterials to serve as a scaffold for novel tissue growth. As adequate vascularization and innervation are essential components for the viability of regenerated tissues, there is a high need for easily accessible stem cells that are capable of supporting these functions. Within the human tooth and its surrounding tissues, different stem cell populations can be distinguished, such as dental pulp stem cells, stem cells from human deciduous teeth, stem cells from the apical papilla, dental follicle stem cells, and periodontal ligament stem cells. Given their straightforward and relatively easy isolation from extracted third molars, dental stem cells (DSCs) have become an attractive source of mesenchymal-like stem cells. Over the past decade, there have been numerous studies supporting the angiogenic, neuroprotective, and neurotrophic effects of the DSC secretome. Together with their ability to differentiate into endothelial cells and neural cell types, this makes DSCs suitable candidates for dental tissue engineering and nerve injury repair.

scholarly journals Dental Tissue — New Source for Stem Cells

2009 ◽  
Vol 9 ◽  
pp. 1167-1177 ◽  
Author(s):  
Vladimir Petrovic ◽  
Vladisav Stefanovic
Keyword(s):  
Stem Cells ◽  
Deciduous Teeth ◽  
Future Research ◽  
Dental Stem Cells ◽  
Dental Tissue ◽  
Periodontal Ligament Stem Cells ◽  
New Findings ◽  
Apical Papilla ◽  
In The Beginning ◽  
Dental Stem Cell

Stem cells have been isolated from many tissues and organs, including dental tissue. Five types of dental stem cells have been established: dental pulp stem cells, stem cells from exfoliated deciduous teeth, stem cells from apical papilla, periodontal ligament stem cells, and dental follicle progenitor cells. The main characteristics of dental stem cells are their potential for multilineage differentiation and self-renewal capacity. Dental stem cells can differentiate into odontoblasts, adipocytes, neuronal-like cells, glial cells, osteoblasts, chondrocytes, melanocytes, myotubes, and endothelial cells. Possible application of these cells in various fields of medicine makes them good candidates for future research as a new, powerful tool for therapy. Although the possible use of these cells in therapeutic purposes and tooth tissue engineering is still in the beginning stages, the results are promising. The efforts made in the research of dental stem cells have clarified many mechanisms underlying the biological processes in which these cells are involved. This review will focus on the new findings in the field of dental stem cell research and on their potential use in the therapy of various disorders.

scholarly journals Wnt and BMP signaling crosstalk in regulating dental stem cells: Implications in dental tissue engineering

2016 ◽  
Vol 3 (4) ◽  
pp. 263-276 ◽  
Author(s):  
Fugui Zhang ◽  
Jinlin Song ◽  
Hongmei Zhang ◽  
Enyi Huang ◽  
Dongzhe Song ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bmp Signaling ◽  
Dental Stem Cells ◽  
Dental Tissue ◽  
Signaling Crosstalk ◽  
Dental Tissue Engineering

scholarly journals Dental Stem Cells and their Applications in Dental Tissue Engineering

2013 ◽  
Vol 7 (1) ◽  
pp. 76-81 ◽  
Author(s):  
S Lymperi ◽  
C Ligoudistianou ◽  
V Taraslia ◽  
E Kontakiotis ◽  
E Anastasiadou
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Tooth Loss ◽  
Main Tool ◽  
Cell Types ◽  
Dental Stem Cells ◽  
Epithelial Stem Cells ◽  
Dental Tissue ◽  
Dental Tissue Engineering ◽  
Missing Tooth

Tooth loss or absence is a common condition that can be caused by various pathological circumstances. The replacement of the missing tooth is important for medical and aesthetic reasons. Recently, scientists focus on tooth tissue engineering, as a potential treatment, beyond the existing prosthetic methods. Tooth engineering is a promising new therapeutic approach that seeks to replace the missing tooth with a bioengineered one or to restore the damaged dental tissue. Its main tool is the stem cells that are seeded on the surface of biomaterials (scaffolds), in order to create a biocomplex. Several populations of mesenchymal stem cells are found in the tooth. These different cell types are categorized according to their location in the tooth and they demonstrate slightly different features. It appears that the dental stem cells isolated from the dental pulp and the periodontal ligament are the most powerful cells for tooth engineering. Additional research needs to be performed in order to address the problem of finding a suitable source of epithelial stem cells, which are important for the regeneration of the enamel. Nevertheless, the results of the existing studies are encouraging and strongly support the belief that tooth engineering can offer hope to people suffering from dental problems or tooth loss.

scholarly journals From pulpal stem cells to tooth repair: an emerging field for dental tissue engineering

2016 ◽  
Vol 1 (1) ◽  
Author(s):  
Anne Baudry ◽  
Emel Uzunoglu ◽  
Benoit Schneider ◽  
Odile Kellermann ◽  
Michel Goldberg
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Tissue ◽  
Dental Tissue Engineering ◽  
Tooth Repair

scholarly journals Dental stem cells and their application in dentistry.

2020 ◽  
Vol 9 (3) ◽  
pp. 220-233
Author(s):  
Heber Arbildo-Vega ◽  
◽  
Fredy Cruzado-Oliva ◽  
Edward Infantes-Ruiz ◽  
◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Stem Cells ◽  
Periodontal Ligament Stem Cells ◽  
Dental Tissues ◽  
Proliferation Capacity ◽  
Apical Papilla ◽  
Many Sources ◽  
Follicle Stem Cells

Recent advances in tissue engineering and regenerative medicine offer a long-term solution through biological repair, replacement of damaged teeth or maintenance and improvement of tissue and organ function through the use of stem cells. Stem cells or also called universal cells, progenitor cells or precursor cells; they are primitive, undifferentiated, clonogenic cells that are characterized by their self-renewal capabilities and that can be differentiated into more specialized cells with specific functions. Currently many sources are known from where you can obtain stem cells, one of which are those obtained from oral or dental tissues, called dental stem cells (DSC), from where it has been possible to identify, isolate and characterize around 8 unique populations: dental pulp stem cells (DPSC), human exfoliated deciduous tooth stem cells (SHED), periodontal ligament stem cells (PLDSC), dental follicle stem cells (DFSC), stem cells derived from bone alveolar (CMHA), the stem cells of the apical papilla (SCAP), the stem cells of the dental germ (DGSC) and the gingival stem cells (GSC). These DSC have attracted attention in recent years due to their accessibility, plasticity and high proliferation capacity. Currently, DSC have shown that they can be used in endodontic and periodontal regenerative therapy, in the regeneration of dentin and bone and in dental bioengineering. Tissue engineering methodologies combined with a greater understanding of the biology of DSCs will provide powerful tools for a broader spectrum of their application in various future therapeutic strategies.

Study on Culture of Human Dental Pulp Stem Cells to Apply in Tissue Engineering

2011 ◽  
Vol 11 ◽  
pp. 13-20 ◽  
Author(s):  
Tran Le Bao Ha ◽  
Doan Nguyen Vu ◽  
To Minh Quan ◽  
Ngoc Phan Kim ◽  
Hung Hoang Tu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Dental Tissue ◽  
Dentin Surface ◽  
Human Dental Pulp ◽  
Dental Tissue Engineering ◽  
High Level

Dental pulp cell research might open a promising application in tooth tissue regeneration. The aim of this study is to establish a protocol for in vitro culture the human dental pulp stem cells to apply in tissue engineering. Human premolar and impacted third molars were collected and disinfected. Dental pulp fragments were cultured with Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) medium supplemented with 10% Fetal Bovine Serum (FBS). Dental pulp stem cells (DPSCs) were identified using proliferation assay, RT-PCR and flow cytometry. Growth of DPSCs on dentin surface was assessed by MTT assay. The study showed that we successfully isolated, cultured and characterized dental pulp cells by outgrowth method. Cultured population of cells expressed in high level of Oct4, CD146, CD90, CD44. DPSCs proliferated on chemically and mechanically treated dentin surface. This research provides important information and a basis for further investigations to establish dental tissue engineering protocols.

scholarly journals Therapeutic potential of dental stem cells

2017 ◽  
Vol 8 ◽  
pp. 204173141770253 ◽  
Author(s):  
Elna Paul Chalisserry ◽  
Seung Yun Nam ◽  
Sang Hyug Park ◽  
Sukumaran Anil
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Biology ◽  
Developmental Process ◽  
Therapeutic Potential ◽  
Deciduous Teeth ◽  
High Plasticity ◽  
Dental Stem Cells ◽  
Periodontal Ligament Stem Cells ◽  
Dental Tissues

Stem cell biology has become an important field in regenerative medicine and tissue engineering therapy since the discovery and characterization of mesenchymal stem cells. Stem cell populations have also been isolated from human dental tissues, including dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla, dental follicle progenitor cells, and periodontal ligament stem cells. Dental stem cells are relatively easily obtainable and exhibit high plasticity and multipotential capabilities. The dental stem cells represent a gold standard for neural-crest-derived bone reconstruction in humans and can be used for the repair of body defects in low-risk autologous therapeutic strategies. The bioengineering technologies developed for tooth regeneration will make substantial contributions to understand the developmental process and will encourage future organ replacement by regenerative therapies in a wide variety of organs such as the liver, kidney, and heart. The concept of developing tooth banking and preservation of dental stem cells is promising. Further research in the area has the potential to herald a new dawn in effective treatment of notoriously difficult diseases which could prove highly beneficial to mankind in the long run.

scholarly journals Challenges and strategy in treatment with exosomes for cell-free-based tissue engineering in dentistry

2021 ◽  
Author(s):  
Ika Dewi Ana ◽  
Anggraini Barlian ◽  
Atik Choirul Hidajah ◽  
Christofora Hanny Wijaya ◽  
Hari Basuki Notobroto ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Large Scale ◽  
Clinical Applications ◽  
Regenerative Therapy ◽  
Research Needs ◽  
Dental Tissue ◽  
Alternative Approach ◽  
Regenerative Dentistry ◽  
Dental Tissue Engineering

In dentistry, problems of craniofacial, osteochondral, periodontal tissue, nerve, pulp or endodontics injuries, and osteoarthritis need regenerative therapy. The use of stem cells in dental tissue engineering pays a lot of increased attention, but there are challenges for its clinical applications. Therefore, cell-free-based tissue engineering using exosomes isolated from stem cells is regarded an alternative approach in regenerative dentistry. However, practical use of exosome is restricted by limited secretion capability of cells. For future regenerative treatment with exosomes, efficient strategies for large-scale clinical applications are being studied, including the use of ceramics-based scaffold to enhance exosome production and secretion which can resolve limited exosome secretory from the cells when compared with the existing methods available. Indeed, more research needs to be done on these strategies going forward.

Sign in / Sign up

Export Citation Format

Share Document