scholarly journals Sorghum [Sorghum bicolor(L.) Moench] Genotypes with Contrasting Polyphenol Compositions Differentially Modulate Inflammatory Cytokines in Mouse Macrophages

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Davina H. Rhodes ◽  
Stephen Kresovich
Keyword(s):  
Cytokine Production ◽  
Raw 264.7 ◽  
Raw 264.7 Macrophages ◽  
Inflammatory Cytokine Production ◽  
Mouse Macrophages ◽  
Health Promoting ◽  
Sorghum Genotypes ◽  
Ethanol Extracts ◽  
Sorghum Grain ◽  
Necrosis Factor

This study sought to characterize and compare anti-inflammatory effects of twenty sorghum accessions with contrasting grain polyphenol concentrations but similar genetic backgrounds (based on a genomewide estimate of relatedness). Cell viability, tumor necrosis factor- (TNF-)α, and interleukin- (IL-) 6 were measured in RAW 264.7 macrophages treated with increasing doses (0, 15, 30, and 60 μg/mL) of sorghum ethanol extracts and stimulated with lipopolysaccharide (LPS). Extract dose had a significant effect on TNF-αand IL-6, with a trend of cytokines decreasing between 0 μg/mL and 15 μg/mL of sorghum extract. Genotype also had a significant effect on the cytokines, with extracts from four accessions significantly decreasing TNF-αand/or IL-6. Cells treated with 3-deoxyanthocyanidin-containing accessions had less cytokine production than non-3-deoxyanthocyanidin accessions, whereas cells treated with proanthocyanidin-containing accessions had more cytokine production than cells treated with nonproanthocyanidin accessions. Additionally, there was a significant effect of theTannin1allele on TNF-αand IL-6. Our results demonstrate that sorghum genotypes differentially modulate induction of inflammatory cytokine production in RAW 264.7 macrophages and that specialty grain has the potential to be tailored by controlling traits at the nucleotide level. This study adds to our knowledge of sorghum health effects and contributes to efforts aimed at developing health-promoting sorghum grain.

Inhibitory effects of cranberry polyphenol and volatile extracts on nitric oxide production in LPS activated RAW 264.7 macrophages

2019 ◽  
Vol 10 (11) ◽  
pp. 7091-7102 ◽  
Author(s):  
Katie Moore ◽  
Luke Howard ◽  
Cindi Brownmiller ◽  
Inah Gu ◽  
Sun-Ok Lee ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Raw 264.7 ◽  
Nitric Oxide Production ◽  
Inhibitory Effects ◽  
Raw 264.7 Macrophages ◽  
Oxide Production ◽  
Health Promoting

Cranberry volatiles have received little attention for health-promoting properties.

scholarly journals Regulation of Inhibitor of Apoptosis Expression by Nitric Oxide and Cytokines: Relation to Apoptosis Induction in Rat Mesangial Cells and RAW 264.7 Macrophages

2001 ◽  
Vol 12 (6) ◽  
pp. 1151-1163
Author(s):  
MARKUS MANDERSCHEID ◽  
UDO K. MEßMER ◽  
ROCHUS FRANZEN ◽  
JOSEF PFEILSCHIFTER
Keyword(s):  
Mesangial Cells ◽  
Inhibitor Of Apoptosis ◽  
No Production ◽  
Raw 264.7 ◽  
Apoptosis Induction ◽  
No Donors ◽  
Raw 264.7 Macrophages ◽  
Protein Levels ◽  
Factor Α ◽  
Necrosis Factor

Abstract. Mesangial cells and RAW 264.7 macrophages respond to different nitric oxide (NO) donors within 16 to 24 h or 6 to 8 h, respectively, with apoptotic cell death. RAW 264.7 macrophages also die in response to endogenous NO production. In contrast, endogenous NO production fails to significantly induce cell death in mesangial cells. It was hypothesized that differences in the expression of antiapoptotic proteins, in particular the inhibitor of apoptosis (IAP) protein family, might be responsible for this cell type-specific behavior. Therefore, IAP expression was investigated in relation to apoptosis induction in response to NO and cytokines in both cell types. In mesangial cells, interleukin-1β (IL-1β) and tumor necrosis factor-α induced cellular inhibitor of apoptosis 1 (cIAP1) mRNA expression within 3 h. In contrast, X chromosome-linked inhibitor of apoptosis (XIAP) mRNA levels remained unaffected by cytokines. Although coincubation of cells with IL-1β and tumor necrosis factor-α or IL-1β and basic fibroblast growth factor resulted in synergistic induction of inducible NO synthase, comparable potentiating effects on cIAP1 induction were absent. Exogenously released NO from NO donors promoted cIAP1 mRNA upregulation in mesangial cells, whereas XIAP mRNA was downregulated. However, the changes observed on the mRNA level were not adequately translated to the protein level, and corresponding values for cIAP1 and XIAP were only slightly affected. In contrast, in lipopolysaccharide/interferon-γ-stimulated RAW 264.7 macrophages, massive NO-dependent downregulation of cIAP1 and XIAP protein levels, which correlated temporally with the induction of apoptosis, was observed. This effect was at least partially reversed by NG-monomethyl-L-arginine, an inhibitor of NO synthase activity. In summary, a direct correlation between the downregulation of IAP protein levels and the induction of apoptosis by endogenous NO was observed in macrophages. In contrast, a stable level of IAP protein in mesangial cells might represent a mechanism for the resistance of the cells to endogenously produced NO.

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