scholarly journals A Comparison of the Effects of Alpha and Medical-Grade Honey Ointments on Cutaneous Wound Healing in Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Shahram Paydar ◽  
Majid Akrami ◽  
Amirreza Dehghanian ◽  
Roshanak Alavi Moghadam ◽  
Mohsen Heidarpour ◽  
...  
Keyword(s):  
Surface Area ◽  
Healing Process ◽  
Study Groups ◽  
Skin Wound ◽  
Collagen Deposition ◽  
Wound Surface ◽  
Sprague Dawley ◽  
Cutaneous Wounds ◽  
Significant Difference ◽  
Medical Grade

Introduction. This study compared the healing efficacy and possible adverse effects of topical Alpha and medical-grade honey ointments on cutaneous wounds in rats. Methods. To conduct the study, 22 male Sprague-Dawley rats were randomly allocated into two equal groups: (1) rats with Alpha ointment applied to the wound surface area and (2) rats with medical-grade honey ointment applied to their wounds. The ointments were applied daily during the 21-day study period. Wound contraction was examined photographically with images taken on days 0, 7, and 21 after wounding. The healing process was histopathologically assessed using skin biopsies taken from the wound sites on days 7 and 21. Results. No statistically significant difference in mean wound surface area was observed between the two study groups. According to histopathological assessment, a significant reduction in the amount of collagen deposition (P value: 0.007) and neovascularisation (P value: 0.002) was seen in the Alpha-treated rats on day 21. No tissue necrosis occurred following the application of Alpha ointment. Conclusion. Daily topical usage of Alpha ointment on a skin wound can negatively affect the healing process by inhibiting neovascularization. Topical Alpha ointment can reduce the possibility of excessive scar formation by reducing collagen deposition.

scholarly journals Structural Alteration in Dermal Vessels and Collagen Bundles following Exposure of Skin Wound to Zeolite–Bentonite Compound

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Shahram Paydar ◽  
Ali Noorafshan ◽  
Behnam Dalfardi ◽  
Shahram Jahanabadi ◽  
Seyed Mohammad Javad Mortazavi ◽  
...  
Keyword(s):  
Healing Process ◽  
Volume Density ◽  
Skin Wound ◽  
Sprague Dawley ◽  
Small Vessels ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Sterile Normal Saline ◽  
Animal Skin ◽  
The Impact

Background. This study examines the impact of one-time direct application of haemostatic agent zeolite–bentonite powder to wounded skin on the healing process in rats. Materials and Methods. 24 male Sprague-Dawley rats were randomly allocated into two groups (n=12): (1) the rats whose wounds were washed only with sterile normal saline (NS-treated) and (2) those treated with zeolite–bentonite compound (ZEO-treated). The wound was circular, full-thickness, and 2 cm in diameter. At the end of the 12th day, six animals from each group were randomly selected and terminated. The remaining rats were terminated after 21 days. Just after scarification, skin samples were excised and sent for stereological evaluation. Results. The results showed a significant difference between the two groups regarding the length density of the blood vessels and diameter of the large and small vessels on the 12th day after the wound was inflicted. Besides, volume density of both the dermis and collagen bundles was reduced by 25% in the ZEO-treated rats in comparison to the NS-treated animals after 21 days. Conclusions. One-time topical usage of zeolite–bentonite haemostatic powder on an animal skin wound might negatively affect the healing process through vasoconstriction and inhibition of neoangiogenesis.

scholarly journals Perbandingan Perubahan Luas Luka dan Angiogenesis pada Jaringan Luka Bakar Derajat IIB Tikus Sprague Dawley yang Diberikan Platelet Rich Fibrin dan Advanced Platelet Rich Fibrin

2021 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Shantika Aqilla Kurnia ◽  
Komang Ardi Wahyuningsih
Keyword(s):  
Surface Area ◽  
Burn Injury ◽  
Healing Process ◽  
Wound Surface ◽  
Sprague Dawley ◽  
Platelet Rich Fibrin ◽  
Sprague Dawley Rats ◽  
Days Of Therapy ◽  
Group A ◽  
Group D

Burns increase tissue damage by free radicals, thus hindering the healing process. Platelet Rich Fibrin (PRF) and Advanced Platelet Rich Fibrin (A-PRF) are autologous fibrin matrices that contain growth factors and have been proven to increase tissue formation and regeneration. Objectives: To determined the effect of applying PRF and A-PRF on deep second-degree burns. Methods: This study was experimental research on twenty male Sprague Dawley rats were randomly divided into four groups. Group A and B as control groups received NaCl 0,9% and silver sulfadiazine ointment, respectively. Group C was given PRF and group D was given A-PRF. Burns are photographed and the area was calculated using the ImageJ application. After 14 days of therapy, the burn areas were excised and made into histological slides. Microscopic study of angiogenesis was observed using a microscope at 400x magnification. Results: Changes in wound surface area in PRF and A-PRF groups were 3,106 cm and 2,333 cm. The number of angiogenesis in PRF and A-PRF groups were 3.88 and 5.88. The data was analyzed using One-Way Anova and the results showed that there were no significant differences between the changes in the wound area and angiogenesis of PRF and A-PRF groups. Conclusion: This study showed that there are no significant differences in changes in wound surface area and angiogenesis of burn injury after topical application of PRF and A-PRF.Keywords:  angiogenesis, burn injury, changes in wound surface area, A-PRF, PRF

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

Leg venous ulcer healing process after application of membranous dressing with silver ions

Phlebologie ◽  
2013 ◽  
Vol 42 (06) ◽  
pp. 340-346 ◽  
Author(s):  
K. Wilemska-Kucharzewska ◽  
M. Kózka ◽  
M. Spałkowska ◽  
M. Kucharzewski
Keyword(s):  
Surface Area ◽  
Healing Process ◽  
Silver Ions ◽  
Lower Leg ◽  
Venous Ulcer ◽  
Wound Surface ◽  
Venous Ulceration ◽  
Wound Area ◽  
Group A ◽  
Group B

SummaryAim: To assess the efficiency of application of new membranous dressing with silver ions in patients with chronic venous ulceration.Methods: 58 patients were divided randomly into two groups. In group A (n=30) mean ulcer surface area was 8.52 cm2, in group B (n=28) mean wound surface area was 8.29 cm2. In group A membranous dressings with silver ions (Textus Bioactiv® by Biocell GmbH, Germany) were used. In group B hydrocolloid dressing Unna’s boot was applied. All dressings were changed every 7 days until the wound was completely healed. Changes in wound area were calculated and the speed of healing process was measured every week with healing per day index (cm2/day).Results: After 7 weeks ulcers healed in all patients of group A. Ulcers in group B healed after 16 weeks of treatment.Conclusion: On the basis of this outcome the authors concluded that application of membranous dressing with silver ions speeds up the process of treating chronic lower leg ulceration.

scholarly journals Non-invasive monitoring of pharmacodynamics during the skin wound healing process using multimodal optical microscopy

2020 ◽  
Vol 8 (1) ◽  
pp. e000974 ◽  
Author(s):  
Jose Rico-Jimenez ◽  
Jang Hyuk Lee ◽  
Aneesh Alex ◽  
Salma Musaad ◽  
Eric Chaney ◽  
...  
Keyword(s):  
Wound Healing ◽  
Optical Imaging ◽  
Second Harmonic ◽  
Healing Process ◽  
Skin Wound ◽  
Metabolic Changes ◽  
Wound Healing Process ◽  
Collagen Deposition ◽  
Diabetic Wound ◽  
Non Invasive

ObjectiveImpaired diabetic wound healing is one of the serious complications associated with diabetes. In patients with diabetes, this impairment is characterized by several physiological abnormalities such as metabolic changes, reduced collagen production, and diminished angiogenesis. We designed and developed a multimodal optical imaging system that can longitudinally monitor formation of new blood vessels, metabolic changes, and collagen deposition in a non-invasive, label-free manner.Research design and methodsThe closure of a skin wound in (db/db) mice, which presents delayed wound healing pathologically similar to conditions in human type 2 diabetes mellitus, was non-invasively followed using the custom-built multimodal microscope. In this microscope, optical coherence tomography angiography was used for studying neovascularization, fluorescence lifetime imaging microscopy for nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) assessment, fluorescence intensity changes of NAD(P)H and flavin adenine dinucleotide (FAD) cofactors for evaluating metabolic changes, and second harmonic generation microscopy for analyzing collagen deposition and organization. The animals were separated into four groups: control, placebo, low concentration (LC), and high concentration (HC) treatment. Images of the wound and surrounding areas were acquired at different time points during a 28-day period.ResultsVarious physiological changes measured using the optical imaging modalities at different phases of wound healing were compared. A statistically significant improvement in the functional relationship between angiogenesis, metabolism, and structural integrity was observed in the HC group.ConclusionsThis study demonstrated the capability of multimodal optical imaging to non-invasively monitor various physiological aspects of the wound healing process, and thus become a promising tool in the development of better diagnostic, treatment, and monitoring strategies for diabetic wound care.

Measurement of mechanical withdrawal threshold on full-thickness cutaneous wounds in rats using the von Frey test

2019 ◽  
Vol 28 (11) ◽  
pp. 762-772
Author(s):  
Taichi Goto ◽  
Gojiro Nakagami ◽  
Takeo Minematsu ◽  
Masamichi Shinoda ◽  
Hiromi Sanada
Keyword(s):  
Full Thickness ◽  
Sprague Dawley ◽  
Wound Edge ◽  
Cutaneous Wounds ◽  
Withdrawal Threshold ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Wound Pain ◽  
Indomethacin Treatment ◽  
Morphine Hydrochloride

Objective: A method for measuring mechanical withdrawal threshold of full-thickness cutaneous wound pain in animal models is lacking. This study aimed to confirm the validity and reactivity of the von Frey test in full-thickness cutaneous wounds in rats. Method: A 1.5cm-diameter wound was established on the dorsal areas of male Sprague-Dawley rats and subcutaneously injected with either morphine hydrochloride (5.0mg/kg) or indomethacin (2.5mg/kg) with a 27-gauge needle on day three post-wounding. On day five post-wounding, an injection of morphine hydrochloride, indomethacin or lambda-carrageenan (1.0%) into the granulation tissue was also administered. The withdrawal threshold of mechanical stimulation of the wound edge was compared in each group before treatment with injection and at two, four, eight and 24 hours after injection. Results: A total of 40 rats were used in the study. Since more severe inflammation in and around the wound was induced on day three post-wounding than that of day five, the withdrawal threshold measured on day three post-wounding was significantly lower than that of day five. The decrease of the withdrawal threshold was depressed by morphine hydrochloride and indomethacin treatment on day three post-wounding. While there was no significant difference between the changes in the withdrawal threshold after indomethacin treatment on day five post-wounding, we observed an increased withdrawal threshold after morphine hydrochloride treatment and decreased withdrawal threshold after lambda-carrageenan treatment on day five post-wounding. Conclusion: The results suggest that the von Frey test can be applied to measure the mechanical withdrawal threshold of full-thickness dorsal wounds in rats.

scholarly journals The Effects of Aloe vera Cream on the Expression of CD4+ and CD8+ Lymphocytes in Skin Wound Healing

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Yos Adi Prakoso ◽  
Kurniasih
Keyword(s):  
Wound Healing ◽  
Topical Application ◽  
Aloe Vera ◽  
Skin Wound ◽  
Control Group ◽  
Histopathological Study ◽  
Sprague Dawley ◽  
Skin Wound Healing ◽  
Group I ◽  
Significant Difference

The aim of this study is to explore the effect of topical application of Aloe vera on skin wound healing. Thirty-six male Sprague-Dawley rats weighing 150–200 grams were divided into four groups. All groups were anesthetized, shaved, and exposed to round full-thickness punch biopsy on the back: group I (control); group II (treated with 1% Aloe vera cream); group III (treated with 2% Aloe vera cream); and group IV (treated with madecassol®). The treatments were given once a day. Macroscopic and microscopic examination were observed at 5, 10, and 15 days after skin biopsy. Skin specimens were prepared for histopathological study using H&E stain and IHC stain against CD4+ and CD8+ lymphocytes. All the data were analyzed using SPSS16. The result showed that topical application of 1% and 2% Aloe vera cream significantly reduced the percentage of the wound, leucocytes infiltration, angiogenesis, and expression of CD8+ lymphocytes and increased the epidermal thickness and the expression of CD4+ lymphocytes (p ≤ 0,05). There was no significant difference in the number of fibroblasts in all groups. Topical application of 1% and 2% Aloe vera cream has wound healing potential via their ability to increase the ratio of CD4+/CD8+ lymphocytes in the wound area.

scholarly journals The combination of carbonate hydroxyapatite and human β-defensin 3 to enhance collagen fibre density in periodontitis Sprague Dawley rats

2020 ◽  
Vol 53 (2) ◽  
pp. 76
Author(s):  
Ika Andriani ◽  
Edy Meiyanto ◽  
S. Suryono ◽  
Ika Dewi Ana
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Healing Process ◽  
Collagen Fibre ◽  
Control Group ◽  
Sprague Dawley ◽  
Carbonate Hydroxyapatite ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Collagen Density

Background: Carbonate hydroxyapatite (CHA) is used as a scaffold to repair bone resorption. Alveolar bone resorption in periodontitis caused by an infection requires the presence of an antibacterial to support bone regeneration. Human β-defensin 3 (HBD3) is an antimicrobial peptide. The local application of the HBD3 antimicrobial is beneficial to inhibiting drug resistance and protecting tissue regeneration against invasive bacteria. Purpose: This study aims to investigate the effect of the administration of a combination of   CHA with HBD3 on the collagen density of periodontitis rats (Sprague Dawley). Methods: This study was a true experimental study with a post-test control group design. Thirty-two Sprague Dawley animal models were randomly blind selected placed under anaesthetic, then a 2-mm silk ligature was attached as a ligation to the mandibular incisors for 14 days in order to generate periodontitis. The study subjects were divided into two groups, the group with CHA and CHA loaded HBD3 (CHA + HBD3) implantation. On days 7, 14, 21 and 28, four rats were taken randomly from each group for decapitation, followed by histological processing and examination with trichome Mallory staining. The data was analysed using the Kruskal Wallis test (p<0.05). Results: An increase in collagen density during the healing process was found. There was a significant difference between CHA and CHA+HBD (p=0.004 and p=0.008; p<0.05) in collagen density between the groups. Conclusion: The combination of CHA and HBD3 can enhance the collagen density in periodontitis Sprague Dawley rats, compared to CHA only groups.

Effects of anticoagulant drugs on wound healing process in a rat model: a comparative study

2020 ◽  
Vol 29 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Deniz Çevirme ◽  
Ömer Faruk Savluk ◽  
Eylül Kafalı Başaran ◽  
Rezzan Aksoy ◽  
Ahmet Elibol ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rat Model ◽  
Healing Process ◽  
Female Rats ◽  
Wound Healing Process ◽  
Sprague Dawley ◽  
Score Distribution ◽  
Significant Difference

Objective: This study aims to compare the efficacy of enoxaparin, rivoraxaban and dabigatran on wound healing using a rat model. Method: Sprague-Dawley female rats (n=56), 10–12 weeks old, weight 245±30g, were used in this study. The rats were divided into four equally-sized groups. A type 1 (secondary wound healing) and type 2 (primary wound healing) wound was opened surgically on each rat in each group. Anticoagulent drugs enoxaparin, rivoraxaban and dabigatran and physiological saline solution were administered to Groups 1, 2, 3 and 4, respectively. After wound healing was scored tissue samples were taken from euthanised rats at days five and 10 and examined histologically. Since time was used as a classification (days five and 10), a time effect was included. Results: There was no statistically significant difference in total score distribution in rats between type 1 secondary wounds for days five and 10 (p>0.05). There was no statistically significant difference in the overall score distribution in rats between type 2 primary wounds for days five and 10 (p>0.05). Conclusion: In addition to the use of low molecular weight heparin with well-known anticoagulation activity, the new generation oral medications are used efficiently in thromboembolic diseases. However, there was no evidence observed in this study that these drugs could be either beneficial or harmful to wound healing.

scholarly journals Hemostatic and Histopathological Effects of Local Mineral Zeolite and Tranexamic Acid in Experimental Femoral Artery Bleeding Model

2021 ◽  
Vol 25 (2) ◽  
pp. 114-121
Author(s):  
Müzeyyen Seda Altop ◽  
Özge Özdal Zincir ◽  
Meral Ünür ◽  
Necat Vakur Olgaç ◽  
Sevgi Özyeğin
Keyword(s):  
Tranexamic Acid ◽  
Femoral Artery ◽  
Exothermic Reaction ◽  
Study Groups ◽  
Female Rats ◽  
Acid Mixture ◽  
Control Group ◽  
Sprague Dawley ◽  
Significant Difference ◽  
And Control

Summary Background/Aim: This study aimed to evaluate the effect of local zeolite and tranexamic acid application on hemostasis duration and histopathological changes in the experimental bleeding model, created by puncturing femoral arteries in rats. Material and Methods: A total of 36 Sprague Dawley female rats weighing an average of 240 ± 20 g were used in the study. The three main study groups were the zeolite, zeolite+tranexamic acid, and control groups. Each group was sacrificed on the seventh and fourteenth days of the study, using subgroups for histopathological findings. After piercing the femoral artery of each rat, one gram of the material assigned to the group was applied to the bleeding site after which a 100-gram scale weight was placed on the site for 30 sec intervals, during which temperature was measured. The same sequence of procedures was repeated for the control group, using only standard compression. Statistical analysis was performed using IBM Statistical Package for Social Sciences (SPSS) 15 statistical software. Significance was evaluated at the level of p< 0.05. Results: The bleeding stop time of the control group was significantly longer than the zeolite and zeolite+tranexamic groups (p< 0.05). There was no statistically significant difference between the zeolite and zeolite+tranexamic groups’ bleeding stop times (p> 0.05) or between the mean wound temperatures of the control and zeolite+tranexamic acid groups when bleeding stopped (p> 0.05). Conclusions: The effectiveness of the zeolite group and zeolite+tranexamic acid mixture is more than the control group in ensuring bleeding control. Their efficacy has been clearly observed in providing hemostasis. In addition, it has been determined that zeolite tranexamic acid mixture causes less exothermic reaction than zeolite group. We believe that this new formula should be developed and used to guide new studies.

Sign in / Sign up

Export Citation Format

Share Document