scholarly journals In VitroCytotoxicity of Calcium Silicate-Based Endodontic Cement as Root-End Filling Materials

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Selen Küçükkaya ◽  
Mehmet Ömer Görduysus ◽  
Naciye Dilara Zeybek ◽  
Sevda Fatma Müftüoğlu
Keyword(s):  
Cell Viability ◽  
Calcium Silicate ◽  
Repeated Measures ◽  
Periodontal Ligament ◽  
Cultured Cells ◽  
Periodontal Ligament Fibroblasts ◽  
Filling Materials ◽  
Significant Difference ◽  
Incubation Periods ◽  
Human Periodontal Ligament Fibroblasts

The aim of this study was to evaluate the cytotoxicity of three types of calcium silicate-based endodontic cement after different incubation periods with human periodontal ligament fibroblasts. Human periodontal ligament fibroblasts were cultured from extracted third molars and seeded in 96-well plates. MTA, calcium enriched mixture (CEM) cement, and Biodentine were prepared and added to culture insert plates which were immediately placed into 96-well plates containing cultured cells. After incubation periods of 24, 48, and 72 hours, cell viability was determined with WST-1 assay. Data were analysed statistically by ANOVA with repeated measures and Bonferroni tests. There was no significant difference in cell viability amongst the test materials after each incubation period (P>0.05). MTA and CEM presented more than 90% cell viability after 24 and 48 hours of incubation and showed statistically significant decrease in cell viability after 72 hours of incubation (P<0.05). Biodentine showed significantly less cell viability (73%) after 24 hours of incubation, whereas more than 90% cell viability was seen after 48 and 72 hours of incubation (P<0.05). Despite the significant changes in cell viability over time, materials presented similar cytotoxicity profile. Biodentine and CEM can be considered as alternative materials for root-end surgery procedures.

scholarly journals In Vitro Cytotoxicity Evaluation of Three Root-End Filling Materials in Human Periodontal Ligament Fibroblasts

2016 ◽  
Vol 27 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Hernán Coaguila-Llerena ◽  
Abraham Vaisberg ◽  
Zulema Velásquez-Huamán
Keyword(s):  
Cell Viability ◽  
Periodontal Ligament ◽  
Colorimetric Assay ◽  
Control Group ◽  
Repair Material ◽  
Periodontal Ligament Fibroblasts ◽  
Filling Materials ◽  
Root Repair ◽  
Human Periodontal Ligament Fibroblasts

Abstract The aim of this study was to evaluate in vitro the cytotoxicity on human periodontal ligament fibroblasts of three root-end filling materials: MTA Angelus(r), EndoSequence Root Repair Material Putty(r) and Super EBA(r). A primary culture of human periodontal ligament fibroblasts was previously obtained in order to evaluate the cytotoxicity of the three extracts from the root-end filling materials after 2 and 7 days of setting. Serial dilutions of these extracts (1:1, 1:2, 1:4 and 1:8) were evaluated at 1, 3 and 7 days using the methyl-thiazol-tetrazolium (MTT) colorimetric assay. Cell viability was evaluated as percentage of the negative control group, which represented 100% cell viability. Statistical analyses were done with t-test, ANOVA and Kruskal-Wallis test at a significance level of 5%. It was found that the main difference among root-end filling materials was in the higher dilutions (p<0.05), but there was a similar behavior in lower dilutions (p>0.05). Cell viability of MTA Angelus(r) was superior for 2-day setting (p<0.05), compared with the other two root-end fillings. There were no statistically significant differences between 7-day set MTA Angelus(r) and EndoSequence Root Repair Material Putty(r). Super EBA(r) showed the lowest percentage of cell viability at higher dilutions (p<0.05). Therefore, MTA Angelus(r) and EndoSequence Root Repair Material Putty(r) were less cytotoxic in the highest dilution (1:1) compared with Super EBA(r).

scholarly journals Effect of Artemisinin-Loaded Mesoporous Cerium-Doped Calcium Silicate Nanopowder on Cell Proliferation of Human Periodontal Ligament Fibroblasts

Nanomaterials ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 2189
Author(s):  
Ioannis Tsamesidis ◽  
Dimitrios Gkiliopoulos ◽  
Georgia K. Pouroutzidou ◽  
Evgenia Lymperaki ◽  
Chrysanthi Papoulia ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Calcium Silicate ◽  
Periodontal Ligament ◽  
High Resolution Mass Spectrometry ◽  
Drug Carriers ◽  
Loading Capacity ◽  
Ros Scavenging ◽  
Periodontal Ligament Fibroblasts ◽  
X Ray ◽  
Human Periodontal Ligament Fibroblasts

Ion doping has rendered mesoporous structures important materials in the field of tissue engineering, as apart from drug carriers, they can additionally serve as regenerative materials. The purpose of the present study was the synthesis, characterization and evaluation of the effect of artemisinin (ART)-loaded cerium-doped mesoporous calcium silicate nanopowders (NPs) on the hemocompatibility and cell proliferation of human periodontal ligament fibroblasts (hPDLFs). Mesoporous NPs were synthesized in a basic environment via a surfactant assisted cooperative self-assembly process and were characterized using Scanning Electron Microscopy (SEM), X-ray Fluorescence Spectroscopy (XRF), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction Analysis (XRD) and N2 Porosimetry. The loading capacity of NPs was evaluated using Ultrahigh Performance Liquid Chromatography/High resolution Mass Spectrometry (UHPLC/HRMS). Their biocompatibility was evaluated with the MTT assay, and the analysis of reactive oxygen species was performed using the cell-permeable ROS-sensitive probe 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA). The synthesized NPs presented a mesoporous structure with a surface area ranging from 1312 m2/g for undoped silica to 495 m2/g for the Ce-doped NPs, excellent bioactivity after a 1-day immersion in c-SBF, hemocompatibility and a high loading capacity (around 80%). They presented ROS scavenging properties, and both the unloaded and ART-loaded NPs significantly promoted cell proliferation even at high concentrations of NPs (125 μg/mL). The ART-loaded Ce-doped NPs with the highest amount of cerium slightly restricted cell proliferation after 7 days of culture, but the difference was not significant compared with the control untreated cells.

A Comparative Study of the Effects of Three Root-end Filling Materials on Proliferation and Adherence of Human Periodontal Ligament Fibroblasts

2011 ◽  
Vol 37 (6) ◽  
pp. 865-870 ◽  
Author(s):  
Athina Samara ◽  
Yvanna Sarri ◽  
Dimitrios Stravopodis ◽  
Giorgos N. Tzanetakis ◽  
Evangelos G. Kontakiotis ◽  
...  
Keyword(s):  
Comparative Study ◽  
Periodontal Ligament ◽  
Periodontal Ligament Fibroblasts ◽  
Filling Materials ◽  
Human Periodontal Ligament Fibroblasts
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