scholarly journals Essential Oil ofOcimum basilicumL. and (−)-Linalool Blocks the Excitability of Rat Sciatic Nerve

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Antonio Medeiros Venancio ◽  
Francisco Walber Ferreira-da-Silva ◽  
Kerly Shamyra da Silva-Alves ◽  
Hugo de Carvalho Pimentel ◽  
Matheus Macêdo Lima ◽  
...  
Keyword(s):  
Nervous System ◽  
Sciatic Nerve ◽  
Essential Oil ◽  
Peripheral Nervous System ◽  
Extracellular Recording ◽  
Compound Action Potential ◽  
Germplasm Bank ◽  
Pharmacological Activities ◽  
Rat Sciatic Nerve ◽  
Compound Action

The racemate linalool and its levogyrus enantiomer [(−)-LIN] are present in many essential oils and possess several pharmacological activities, such as antinociceptive and anti-inflammatory. In this work, the effects of essential oil obtained from the cultivation of theOcimum basilicumL. (EOOb) derived from Germplasm Bank rich in (−)-LIN content in the excitability of peripheral nervous system were studied. We used rat sciatic nerve to investigate the EOOb and (−)-LIN effects on neuron excitability and the extracellular recording technique was used to register the compound action potential (CAP). EOOb and (−)-LIN blocked the CAP in a concentration-dependent way and these effects were reversible after washout. EOOb blocked positive amplitude of 1st and 2nd CAP components with IC50of0.38±0.2and0.17±0.0 mg/mL, respectively. For (−)-LIN, these values were0.23±0.0and0.13±0.0 mg/mL. Both components reduced the conduction velocity of CAP and the 2nd component seems to be more affected than the 1st component. In conclusion EOOb and (−)-LIN inhibited the excitability of peripheral nervous system in a similar way and potency, revealing that the effects of EOOb on excitability are due to the presence of (−)-LIN in the essential oil.

Effects of essential oil of Alpinia zerumbet on the compound action potential of the rat sciatic nerve

Phytomedicine ◽  
2004 ◽  
Vol 11 (6) ◽  
pp. 549-553 ◽  
Author(s):  
J.H. Leal-Cardoso ◽  
M.R. Moreira ◽  
G.M. Pinto da Cruz ◽  
S.M. de Morais ◽  
M.S. Lahlou ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Essential Oil ◽  
Action Potential ◽  
Compound Action Potential ◽  
Rat Sciatic Nerve ◽  
Alpinia Zerumbet ◽  
Compound Action

scholarly journals Effects of terpineol on the compound action potential of the rat sciatic nerve

2001 ◽  
Vol 34 (10) ◽  
pp. 1337-1340 ◽  
Author(s):  
M.R. Moreira ◽  
G.M.P. Cruz ◽  
M.S. Lopes ◽  
A.A.C. Albuquerque ◽  
J.H. Leal-Cardoso
Keyword(s):  
Sciatic Nerve ◽  
Action Potential ◽  
Compound Action Potential ◽  
Rat Sciatic Nerve ◽  
Compound Action

scholarly journals Effects of estragole on the compound action potential of the rat sciatic nerve

2004 ◽  
Vol 37 (8) ◽  
pp. 1193-1198 ◽  
Author(s):  
J.H. Leal-Cardoso ◽  
B.G. Matos-Brito ◽  
J.E.G. Lopes-Junior ◽  
K.V. Viana-Cardoso ◽  
A.B. Sampaio-Freitas ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Action Potential ◽  
Compound Action Potential ◽  
Rat Sciatic Nerve ◽  
Compound Action

scholarly journals Essential Oil of Croton zehntneri Prevents Conduction Alterations Produced by Diabetes Mellitus on Vagus Nerve

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 893
Author(s):  
Kerly Shamyra Silva-Alves ◽  
Francisco Walber Ferreira-da-Silva ◽  
Andrelina Noronha Coelho-de-Souza ◽  
José Henrique Leal-Cardoso
Keyword(s):  
Diabetes Mellitus ◽  
Essential Oil ◽  
Diabetic Neuropathy ◽  
Vagus Nerve ◽  
Therapeutic Agent ◽  
Specific Treatment ◽  
Compound Action Potential ◽  
Unmyelinated Fibers ◽  
Conduction Velocities ◽  
Compound Action

Autonomic diabetic neuropathy (ADN) is a complication of diabetes mellitus (DM), to which there is no specific treatment. In this study, the efficacy of the essential oil of Croton zehntneri (EOCz) in preventing ADN was evaluated in the rat vagus nerve. For the two fastest conducting myelinated types of axons of the vagus nerve, the conduction velocities and rheobase decreased, whilst the duration of the components of the compound action potential of these fibers increased. EOCz completely prevented these DM-induced alterations of the vagus nerve. Unmyelinated fibers were not affected. In conclusion, this investigation demonstrated that EOCz is a potential therapeutic agent for the treatment of ADN.

scholarly journals Effects of Lippia sidoides essential oil, thymol, p-cymene, myrcene and caryophyllene on rat sciatic nerve excitability

2017 ◽  
Vol 50 (12) ◽  
Author(s):  
R. Barbosa ◽  
Y. Cruz-Mendes ◽  
K.S. Silva-Alves ◽  
F.W. Ferreira-da-Silva ◽  
N.M. Ribeiro ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Essential Oil ◽  
Lippia Sidoides ◽  
Rat Sciatic Nerve ◽  
Nerve Excitability

Effect of Glucose on Isolated Rat Sciatic Nerve Compound Action Potentials

2000 ◽  
Vol 38 (5) ◽  
pp. 871
Author(s):  
Cheong Lee ◽  
Hee Jung Jun ◽  
Jae Hong Park ◽  
Sam Soon Cho ◽  
Yoon Choi
Keyword(s):  
Sciatic Nerve ◽  
Action Potentials ◽  
Rat Sciatic Nerve ◽  
Compound Action Potentials ◽  
Effect Of Glucose ◽  
Isolated Rat ◽  
Compound Action

Expression of HGF and cMet in the peripheral nervous system of adult rats following sciatic nerve injury

Neuroreport ◽  
2001 ◽  
Vol 12 (7) ◽  
pp. 1403-1407 ◽  
Author(s):  
Norio Hashimoto ◽  
Hiroki Yamanaka ◽  
Tetsuo Fukuoka ◽  
Yi Dai ◽  
Koichi Obata ◽  
...  
Keyword(s):  
Nervous System ◽  
Sciatic Nerve ◽  
Peripheral Nervous System ◽  
Nerve Injury ◽  
Sciatic Nerve Injury ◽  
Adult Rats

scholarly journals Sampling and Evaluating the Peripheral Nervous System

2019 ◽  
Vol 48 (1) ◽  
pp. 10-18 ◽  
Author(s):  
Mark T. Butt
Keyword(s):  
Spinal Cord ◽  
Nervous System ◽  
Sciatic Nerve ◽  
Peripheral Nervous System ◽  
Morphological Changes ◽  
Sampling Strategy ◽  
Mechanisms Of Toxicity ◽  
Test Article ◽  
Ganglion Neurons ◽  
The Brain

Many preclinical investigations limit the evaluation of the peripheral nervous system (PNS) to paraffin-embedded sections/hematoxylin and eosin–stained sections of the sciatic nerve. This limitation ignores several key mechanisms of toxicity and anatomic differences that may interfere with an accurate assessment of test article effects on the neurons/neurites peripheral to the brain and spinal cord. Ganglion neurons may be exposed to higher concentrations of the test article as compared to neurons in the brain or spinal cord due to differences in capillary permeability. Many peripheral neuropathies are length-dependent, meaning distal nerves may show morphological changes before they are evident in the mid-sciatic nerve. Paraffin-embedded nerves are not optimal to assess myelin changes, notably those leading to demyelination. Differentiating between axonal or myelin degeneration may not be possible from the examination of paraffin-embedded sections. A sampling strategy more consistent with known mechanisms of toxicity, atraumatic harvest of tissues, optimized fixation, and the use of resin and paraffin-embedded sections will greatly enhance the pathologist’s ability to observe and characterize effects in the PNS.

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