scholarly journals Effects ofStreptococcus bovisIsolated from Bovine Rumen on the Fermentation Characteristics and Nutritive Value of Tanzania Grass Silage

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Anderson de Moura Zanine ◽  
Emerson Alencar Bonelli ◽  
Alexandre Lima de Souza ◽  
Daniele de Jesus Ferreira ◽  
Edson Mauro Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Protein Recovery ◽  
Ammoniacal Nitrogen ◽  
Streptococcus Bovis ◽  
Grass Silage ◽  
Colony Forming Units

This study aimed to evaluate the effects ofStreptococcus bovison the fermentation characteristics and nutritive value of Tanzania grass silage. Tanzania grass was chopped and left untreated (U) or treated withStreptococcus bovisJB1 at 1 × 106colony-forming units per gram (cfu/g) of fresh forage orStreptococcus bovisHC5 at 1 × 106 cfu/g of fresh forage and packed into sixtuplicate laboratory silos. The largest number of enterobacteria, molds and yeast (M&Y) occurred in untreated silages and the smallest populations of enterobacteria and M&Y and the largest numbers of lactic acid bacteria (LAB), at 9.81 and 9.87 log cfu/g, were observed inStreptococcus bovisJB1 and HC5, respectively (P<0.05). Silages treated with JB1 and HC5 had lower (P<0.05) silage pHs and concentrations of ammoniacal nitrogen (NH3-N) than untreated silages. The application ofStreptococcus bovisJB1 and HC5 resulted in fewer losses through gases and effluents (P<0.05), which resulted in greater dry matter recovery (DMR) and crude protein recovery (CPR) (P<0.05).Streptococcus bovisJB1 and HC5 improved the fermentative profile and increased the concentration of crude protein and DMR and CPR in Tanzania grass silage.

The effect of biological additives on the composition and nutritive value of silage

1990 ◽  
Vol 1990 ◽  
pp. 139-139
Author(s):  
M Gonzalez Yanez ◽  
R Mcginn ◽  
D H Anderson ◽  
A R Henderson ◽  
P Phillips
Keyword(s):  
Cell Wall ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Nutritive Value ◽  
Enzyme System ◽  
Lolium Perenne L ◽  
Grass Silage ◽  
Forage Harvester

It Is claimed that the use of the correct enzyme system as an additive on grass silage will satisfactorily control the fermentation and reduce the cell-wall fibre content, thus preserving the nutrients In the silage and aiding their utilisation by the animal (Henderson and McDonald, 1977; Huhtanen et al, 1985; Raurama et al, 1987; Chamberlain and Robertson, 1989; Gordon, 1989;).The aim of the present experiment was to assess the effect of biological additives, enzymes or a combination of enzymes with an Inoculum of lactic acid bacteria, on the composition of silage and on its nutritive value when offered to store lambs as the sole constituent of their diet.On 1st June 1988, first cut perennial ryegrass (Lolium perenne L) at pre-ear emergence was ensiled direct cut untreated (U), treated with a commercial enzyme (E) or with a commercial inoculum of lactic acid bacteria with enzymes (I) in 6t capacity bunker silos. The grass was cut with a mower and lifted with a New Holland precision chop forage harvester. The additives were pumped onto the grass using a dribble bar sited over the pick-up drum.

scholarly journals Evaluasi Kandungan Nutrisi Pakan dan Daya Hambat Tepung Biji Asam Kandis (Gracinia cowa) sebagai Bahan Pakan Unggas

2018 ◽  
Vol 5 (2) ◽  
pp. 20
Author(s):  
Febri Puska Padang ◽  
Osfar Sjofjan ◽  
Edhy Sudjarwo
Keyword(s):  
Escherichia Coli ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Crude Protein ◽  
Dry Matter ◽  
Laboratory Experiments ◽  
Proximate Analysis ◽  
Food Ingredient ◽  
Feed Ingredients ◽  
Inhibitory Bacteria

Kandis acid (Gracinia cowa) has been used for cooking, medicine, beauty, etc., but using of seeds has not been profitable so it was wasted. Seeds can be used as a fitobiotic to replace antibiotics or as feed ingredients. The purpose of this study was to determine the food ingredient and the inhibitory of bacteria on kandis seeds flour. This research was conducted by laboratory experiments using Kandis seeds flour as a material, analysis proximate used to represent the composition of feedstuft, bioactive used test flavonoid quantitaf seconder and the inhibitory test with hollow diffusion methode. The results of the test were dry matter 89.11%, crude fat 4.77, fiber 18.57, crude protein 0.99, tannin 0.29%, GE 5244 kcal / kg, Ca 0.72%, P 0, 22%, flavonoids 0.44%, density 413 g / mL. The smallest inhibitory bacteria produced by lactic acid bacteria then Salmonella and Escherichia coli. The food ingredient represented that value Kandis acid seeds flour can be used as food and there are bacterial inhibitory by the activity of flavonoids and tannins. The conclusion is the kandis acid seeds have potential as feed ingredients and phytobiotics Keywords: gracinia cowa, flavonoid, proximate analysis, inhibition

Effects of adding a Lactobacillus Plantarum inoculant to grass ensiled at different dry matter concentrations and offered to beef cattle

1994 ◽  
Vol 1994 ◽  
pp. 115-115 ◽  
Author(s):  
P. O'Kiely
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Beef Cattle ◽  
Lactobacillus Plantarum ◽  
Dry Matter ◽  
Nutritive Value ◽  
Silage Fermentation ◽  
Different Levels

Silage fermentation is progressively restricted as the extent of pre-wilting increases (O'Kiely et_al., 1988). The magnitude of the improvement in silage nutritive value in response to a lactic acid bacteria inoculant could be related to the extent of the fermentation in the untreated silage. The objective of this experiment was to determine if the response in silage nutritive value to a Lactobacillus plantarum inoculant was similar at different levels of dry matter (DM) concentration.

scholarly journals The effect of lactic acid additive on the quality and chemical composition of meadow grass silage

2003 ◽  
Vol 19 (3-4) ◽  
pp. 61-69
Author(s):  
Jan Pyś ◽  
Wladislaw Migdal ◽  
Branislav Zivkovic ◽  
Olga Kosovac ◽  
Mihal Fabjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Water Solution ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Total N ◽  
Grass Silage ◽  
Fermentation Quality ◽  
Dominant Grasses

In the experiment, forage from the 1st cut of meadow grasses mown at the heading stage of dominant grasses was ensiled. The control silages were made from fresh forage (dry matter 223.5 g?kg-1) and wilted forage (dry matter 351.9 g?kg-1). The experimental silages were made from fresh forages supplemented with a 5% water solution of lactic acid (0.5 and 0.7 l?100 kg-1 of forage) and 8% water solution of lactic acid (0.4 and 0.6 l?100 kg-1 of forage). The highest contents of crude protein, water-soluble carbohydrates and energy were found in silages supplemented with 5% lactic acid applied at 0.7 l?100 kg-1 of forage and in silages supplemented with 8% lactic acid applied at 0.6 l?100 kg-1 of forage. The above silage variants were also characterized by the lowest content of NH3-N in total-N, butyric acid and acetic acid, as well as the highest contents of lactic acid and the best indicators of fermentation quality. No important differences were found between silages made from wilted forage and silages made with lactic acid supplement.

The effect of bacterial inoculants on kikuyu silage quality

1994 ◽  
Vol 122 (1) ◽  
pp. 53-60 ◽  
Author(s):  
M. De Figueiredo ◽  
J. P. Marais
Keyword(s):  
South Africa ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Content ◽  
Crude Protein ◽  
Dry Matter ◽  
Bacterial Inoculants ◽  
Lactic Acid Content ◽  
Kikuyu Grass ◽  
Silage Quality

SUMMARYTwo commercial bacterial inoculants (Lacto-flora and Ecosyl) were added to kikuyu grass (Pennisetum clandestinum) at ensiling, in 1985 and 1988 respectively, at Cedara, South Africa, using laboratory silos. In 1985 (Expt 1), Lacto-flora was added on its own and in combination with the enzymes Celluclast or SP249. In 1988 (Expt 2), Ecosyl was added on its own and in combination with molasses, at two levels of addition.The addition of Lacto-flora alone did not significantly increase the lactic acid bacteria or the lactic acid content of the treated silage. However, treated silage contained 53·6% less iso-butyric acid and 53·7% less ammonia than the control silage. Kikuyu silage supplemented with a combination of Lacto-flora and Celluclast or SP249 had higher numbers of lactic acid bacteria at ensiling (0·231 × 108/ml) than the control silage or silage receiving Lacto-flora alone. However, only silage supplemented with the combination of Lacto-flora and Celluclast had a significantly higher lactic acid content (2·23 compared with 0·04, 0·18 and 0·13% DM for the control silage, silages with Lactoflora and with a combination of Lacto-flora +SP249, respectively). Nevertheless, this silage contained 19·78% more acetic than lactic acid.Silage supplemented with Ecosyl on its own did not differ significantly in digestibility, loss of dry matter, ammonia, total non-structural carbohydrates, lactic acid and crude protein, from the untreated silage. A significant decrease in silage pH (from 5·08 to 4·70) was observed when Ecosyl was added together with molasses at the higher level of addition only. However, no other benefit was obtained by the addition of Ecosyl in combination with molasses.

scholarly journals Chemical composition and nutrient degradability in elephant grass silage inoculated with Streptococcus bovis isolated from the rumen

2014 ◽  
Vol 86 (1) ◽  
pp. 465-474 ◽  
Author(s):  
DANIELE J. FERREIRA ◽  
ANDERSON M. ZANINE ◽  
ROGÉRIO P. LANA ◽  
MARINALDO D. RIBEIRO ◽  
GUILHERME R. ALVES ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Soluble Fraction ◽  
Elephant Grass ◽  
Streptococcus Bovis ◽  
Grass Silage ◽  
Randomized Design ◽  
Fermentation Profile ◽  
Streptococcus Bovis Hc5

The objective of the present study was to assess the chemical and bromatological composition and in situ degradability of elephant grass silages inoculated with Streptococcus bovis isolated from cattle rumen. A complete randomized design was used with four treatments and six replications: elephant grass silage, elephant grass silage inoculated with 106 CFU/g Streptococcus bovis JB1 strains; elephant grass silage inoculated with 106 CFU/g Streptococcus bovis HC5 strains; elephant grass silage inoculated with 106 CFU/g Enterococcus faecium with six replications each. The pH and ammoniacal nitrogen values were lower (P<0.05) for the silages inoculated with Streptococcus bovis JB1 and HC5, respectively. The silage inoculated with Streptococcus bovis had a higher crude protein content (P<0.05) and there were no differences for the fiber contents in the silage. The (a)soluble fraction degradability, especially in the silages inoculated with Streptococcus bovis JB1 and HC5, had higher values, 30.77 and 29.97%, for dry matter and 31.01 and 36.66% for crude protein, respectively. Inoculation with Streptococcus bovis improved the fermentation profile, protein value and rumen degradability of the nutrients.

scholarly journals Intake, nutrients digestibility and nitrogen balance of elephant grass silages with mango by-product addition

2010 ◽  
Vol 39 (1) ◽  
pp. 74-80 ◽  
Author(s):  
Margareth Maria Teles Rêgo ◽  
José Neuman Miranda Neiva ◽  
Aníbal Coutinho do Rêgo ◽  
Magno José Duarte Cândido ◽  
Arnaud Azevêdo Alves ◽  
...  
Keyword(s):  
Nitrogen Balance ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Neutral Detergent Fiber ◽  
Elephant Grass ◽  
Acid Detergent Fiber ◽  
Linear Effect ◽  
Grass Silage ◽  
Total Carbohydrates

The objective of this study was to evaluate the nutritive value of elephant grass silages with increasing levels of dried mango by-product (DMB). Five addition levels were studied (0, 4, 8, 12 and 16%) using 20 rams, in a randomized complete design with five treatments (addition levels) and four replications. Dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), ether extract (EE), total carbohydrates (TC), non-fibrous carbohydrates (NFC) intake and digestibility were evaluated, as well as the total digestible nutrients (TDN) and the silage nitrogen balance (NB). Mango by-product addition did not influence the DM, CP and TC intakes, the TDN level or the OM, CT and NFC digestibilities. On the other hand, DMB addition reduced the NDF and the ADF intakes, as well as the DM, CP, NDF and ADF digestibilities, and the NB of the silages. There was also a increasing linear effect of DMB addition on the EE and NFC intakes, and on EE digestibility. The elephant grass silage with DMB by-product may not be used as a single feed to ruminants because it reduces the NDF and the ADF intakes and the DM, CP, NDF and ADF digestibilities, as well as the nitrogen balance.

scholarly journals Keamanan Susu Fermentasi Yang Beredar Di Banda Aceh Berdasarkan Nilai Gizi dan Jumlah Bakteri Pathogen

2010 ◽  
Vol 10 (1) ◽  
pp. 32-36
Author(s):  
Yusdar Zakaria ◽  
Cut Intan Novita ◽  
Mira Delima
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Crude Protein ◽  
Nutritive Value ◽  
Fermented Milk ◽  
Milk Products ◽  
Safety Condition ◽  
Ph Level ◽  
Banda Aceh ◽  
Fermented Milk Products

Safety condition of commercial fermented milk that put on the market in banda aceh based on nutritive value and pathogenic bacteriaABSTRACT. Fermented milk has much value primarily for the health of digestive system. However without hygienically and sterilized treatment it can become toxic to consumers. This research was conducted to observe nutritive value, the present of pathogenic bacteria, and the safety condition of commercial fermented milk that put on the market in Banda Aceh. Samples of commercial fermented milk tested were obtained from recognized retailers in Banda Aceh. Factorial completely randomized design with six replications was applied in this research. Variety of fermented milk products (yoghurt and drink yoghurt) was the first factor to be analyzed, and the second one was expired date of the products (the date of production and the date of by expired). The amount of crude protein and lipid, pH level, and the amount of lactic acid bacteria, Coliform and Staphilococcus aureus were the parameter determined. The variety and the expired date of the products influenced the amount of crude protein and lipid, the pH level, the amount of lactic acid bacteria, Coliform and Staphilococcus aureus in the level of highly significant different (P0,01). There was also found in highly significant interaction (P0,01) between factors that influenced the amount of lactic acid bacteria and Staphilococcus aureus. On the other hand, the amount of Coliform was not influenced by those factors. The study also results in decreasing of the amount of crude protein and lipid as well as lactic acid bacteria caused by the ongoing time of storing. The longer fermented milk was stored, the lower the level of crude protein, lipid and lactic acid bacteria presented. The lipid level of fermented milk products those put on the market in Banda Aceh have been complied with the requirement standard of SNI 01-2981-1992. However, crude protein level has not achieved the requirement standard yet. Due to the amount of Staphylococcus aureus present, the fermented milk products tested, in fact, were not saved to be consumed. On the contrary, although Coliform was also present, the products were in the category of saved to be consumed.

scholarly journals Conservation Characteristics and Nutritive Value of Sunflower Silages as Affected by The Maturity Stages and Fibrolytic Enzymes

2016 ◽  
Vol 4 (6) ◽  
pp. 464 ◽  
Author(s):  
Sibel Erdoğan ◽  
Murat Demirel
Keyword(s):  
Lactic Acid ◽  
Crude Protein ◽  
Dry Matter ◽  
Nutritive Value ◽  
Enzyme Treatment ◽  
Maturity Stage ◽  
Fibrolytic Enzymes ◽  
Structural Carbohydrate ◽  
Silage Quality ◽  
Dough Stage

Silage quality and nutritive value of sunflower silages ensiled with different level of fibrolytic enzymes at blooming, milk and dough stages were investigated. Fibrolytic enzyme complex (Viscozyme® L, V2010 Sigma Novozyme) was applied 0, 1, 1.5 and 2.5 ml/kgDM at ensiling. The dry matter (DM) yield increased with each increment of the maturity stage. Dry matter (DM), ether extract (EE) and crude cellulose (CC) contents of silages increased also in each delay in harvesting, while crude protein (CP), NDF and ADF contents of silages were the highest in blooming stage. The pH was higher in sunflower silage harvested at dough stage when compared to blooming stage, while acetic, propionic and butyric acid concentrations were all higher in blooming stage, although there were no differences in lactic acid concentrations among harvesting periods. Although silage structural carbohydrate composition was not significantly affected by any enzyme dose, Dose III enzyme treatment at the dough was associated with the highest concentration of lactic acid. Overall, there was no profound effect of enzyme supplementation on nutritive value and silage characteristics of sunflower silage.

EFFECT OF ADDITIVES CONTAINING LACTIC ACID BACTERIA AND/OR HYDROLYTIC ENZYMES WITH AN ANTIOXIDANT UPON THE PRESERVATION OF CORN OR ALFALFA SILAGE

1981 ◽  
Vol 61 (3) ◽  
pp. 669-680 ◽  
Author(s):  
J. G. BUCHANAN-SMITH ◽  
Y. T. YAO
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Crude Protein ◽  
Dry Matter ◽  
Hydrolytic Enzymes ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Total N ◽  
Alfalfa Silage ◽  
Water Soluble Carbohydrate

A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity). The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH4+-N expressed as a percent of total N. Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined, but recoveries of dry matter and crude protein were not consistently affected. Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos. The experimental design used a4 (DM level) × 4 (added glucose — 0,4,8 and 12%, DM basis) × 3 (additive — control, a hydrolytic enzyme/antioxidant additive (E/AO), and E/AO plus lactic acid bacteria (E/AO +)) factorial arrangement of treatments. Alfalfa was harvested using farm equipment. For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N. Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not. Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented. Thus an additive containing an antioxidant and hydrolytic enzymes was not very effective and numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation.

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