scholarly journals Sexual Dimorphism in the Regulation of Estrogen, Progesterone, and Androgen Receptors by Sex Steroids in the Rat Airway Smooth Muscle Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Abraham Zarazúa ◽  
Aliesha González-Arenas ◽  
Gabriela Ramírez-Vélez ◽  
Blanca Bazán-Perkins ◽  
Christian Guerra-Araiza ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Sex Hormones ◽  
Airway Smooth Muscle ◽  
Hormone Receptors ◽  
Muscle Cells ◽  
Sex Hormone ◽  
Male Rats ◽  
Airway Smooth Muscle Cells

The role of sex hormones in lung is known. The three main sex steroid receptors, estrogen, progesterone, and androgen, have not been sufficiently studied in airway smooth muscle cells (ASMC), and the sex hormone regulation on these receptors is unknown. We examined the presence and regulation of sex hormone receptors in female and male rat ASMC by Western blotting and flow cytometry. Gonadectomized rats were treated with 17β-estradiol, progesterone, 17β-estradiol + progesterone, or testosterone. ASMC were enzymatically isolated from tracheas and bronchi. The experiments were performed with double staining flow cytometry (anti-α-actin smooth muscle and antibodies to each hormone receptor). ERα, ERβ, tPR, and AR were detected in females or males. ERαwas upregulated by E2 and T and downregulated by P4 in females; in males, ERαwas downregulated by P4, E + P, and T. ERβwas downregulated by each treatment in females, and only by E + P and T in males. tPR was downregulated by P4, E + P, and T in females. No hormonal regulation was observed in male receptors. AR was downregulated in males treated with E + P and T. We have shown the occurrence of sex hormone receptors in ASMC and their regulation by the sex hormones in female and male rats.

Sex hormone receptors in varicose veins of women with premenstrual syndrome

Phlebologie ◽  
2008 ◽  
Vol 37 (02) ◽  
pp. 68-72 ◽  
Author(s):  
L. Dzieciuchowicz ◽  
M. Kotwicka ◽  
M. Gabriel ◽  
A. Szczęśniak-Chmielecka ◽  
B. Krasińska ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Sex Hormones ◽  
Hormone Receptors ◽  
Varicose Veins ◽  
Progesterone Receptors ◽  
Muscle Cells ◽  
Sex Hormone ◽  
Estrogen And Progesterone Receptors ◽  
Premenstrual Tension

SummaryThe pathogenesis of premenstrual tension syndrome is not fully understood. It has been hypothesized that the interaction between sex hormones and target organs is the key event in its pathogenesis. Aim: The purpose of the study was to examine the differences in the prevalence of smooth muscle cells with sex hormones receptors in varicose veins of women with and without premenstrual tension syndrome (PS). Patients, methods: Samples of great saphenous vein were obtained from 50 women during varicose vein surgery. They were divided into group I (20 women with clinically diagnosed PS) and group II (30 women without PS). Estrogen and progesterone receptors were detected with an immunohistochemical method. Superficial densities of smooth muscle cells positive to estrogen and progesterone receptors were analyzed with densitometric program IM-AN and automatic image analyzer Magicall. The results were compared with t-Student test. Results: There were no differences in superficial density of smooth muscle cells positive to estrogen receptors between the groups. In contradiction to this, superficial density of smooth muscle cells positive to progesterone receptors was higher in group with PS than in patients without, 343 (±171) and 240 (±123), respectively. The difference was statistically significant (p < 0.05). Conclusion: The amount of cells with sex hormone receptors, not the level of hormones, could play a role in PS pathology. Our results show that progesterone is more important than estrogen in effector organs.

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