scholarly journals Comparison of Antimicrobial Activity of Chlorhexidine, Coconut Oil, Probiotics, and Ketoconazole onCandida albicansIsolated in Children with Early Childhood Caries: An In Vitro Study

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Beena Shino ◽  
Faizal C. Peedikayil ◽  
Shyamala R. Jaiprakash ◽  
Gufran Ahmed Bijapur ◽  
Soni Kottayi ◽  
...  

Background. Early childhood caries (ECC) is associated with early colonisation and high levels of cariogenic microorganisms. WithC. albicansbeing one of those, there is a need to determine the effectiveness of various chemotherapeutic agents against it. The study is aimed at isolatingCandidaspecies in children with ECC and at studying the antifungal effect of coconut oil, probiotics,Lactobacillus, and 0.2% chlorhexidine onC. albicansin comparison with ketoconazole.Materials and Methods. Samples were collected using sterile cotton swabs, swabbed on the tooth surfaces from children with ECC of 3 to 6 yrs and streaked on Sabouraud dextrose agar (HI Media) plates and incubated in a 5% CO2enriched atmosphere at 37°C for 24 hours.Candidawas isolated and its susceptibility to probiotics, chlorhexidine, ketoconazole, and coconut oil was determined using Disc Diffusion method.Results. The mean zone of inhibition for chlorhexidine was 21.8 mm, whereas for coconut oil it was 16.8 mm, for probiotics it was 13.5 mm, and for ketoconazole it was 22.3 mm. The difference between the groups was not statistically significant (Chi-square value 7.42,Pvalue 0.06).Conclusion. Chlorhexidine and coconut oil have shown significant antifungal activity which is comparable with ketoconazole.

2021 ◽  
pp. 1-9
Author(s):  
Sivasubramanian Abirami ◽  
Noopur Panchanadikar ◽  
Murugan Satta Muthu ◽  
Suganya Balasubramanian ◽  
Jyotsna Murthy ◽  
...  

The study aimed to evaluate the effectiveness of sustained interventions in children with cleft lip and palate (CLP) for preventing early childhood caries (ECC). This prospective, nonrandomized interventional cohort study was conducted in infants aged 0–12 months with congenital CLP. Interventions were given to parents/primary caregivers in the form of combined oral health-care measures (sterile wet gauze piece, finger brush, toothbrush, and toothpaste) by a motivational interviewing approach. Education of primary caregivers on oral hygiene was provided by audiovisual aids and demonstration. Reinforcement of the prescribed regimen was done through daily short message services in caregivers’ preferred language and bimonthly telephone calls. Participants were followed up for 9–32 months from the time of recruitment, with a mean period of 18.3 ± 5.1 months. Rates of dental caries were represented as prevalence rates, incidence density, and transitional probability. The distribution of the International Caries Detection and Assessment System (ICDAS) scores on different tooth surfaces affected in the intervention group was compared descriptively with that of the age- and sex-matched historical control groups. On analysis of surface-wise distribution of the ICDAS scores in the intervention group (<i>n</i> = 1,919), 1.2% (<i>n</i> = 24) had noncavitated lesions (ICDAS codes 1 and 2), 0.88% (<i>n</i> = 17) had cavitated lesions (ICDAS codes 3–6), and 0.26% (<i>n</i> = 5) had both cavitated and noncavitated lesions (ICDAS codes 1–6). The incidence density of caries-affected children observed at the first and last follow-ups was 1.2 persons/100 person-months and 1.3 persons/100 person-months of observation, respectively. The incidence density of new caries-affected tooth surfaces at the first and last follow-ups was 0.163 surfaces/100 surface-months and 0.062 surfaces/100 surface-months, respectively. Maxillary first molars had the maximum transition from sound to the cavitated lesion (11.5%), followed by maxillary incisors from sound to noncavitated (7.5%) at the last follow-up. Based on the newly developed assessment criteria in our study, sustained interventions proved to be significantly effective in preventing ECC in children with CLP.


2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Balakrishnan Kalpana ◽  
Puniethaa Prabhu ◽  
Ashaq Hussain Bhat ◽  
Arunsaikiran Senthilkumar ◽  
Raj Pranap Arun ◽  
...  

AbstractDental caries is the most prevalent oral disease affecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidification due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were significantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with significant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of biofilm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound biofilm formation and augmented the growth and enhanced biofilm formation in S. mutans in both dual and multispecies cultures.


2020 ◽  
Vol 111 (4) ◽  
pp. 821-828 ◽  
Author(s):  
Gemma Devenish ◽  
Aqif Mukhtar ◽  
Andrea Begley ◽  
A John Spencer ◽  
W Murray Thomson ◽  
...  

ABSTRACT Background Recent reviews have proposed a causal relationship between prolonged breastfeeding and early childhood caries (ECC), but the evidence to date is inconsistent, with few cohort studies and limited investigation of key confounders. Objective This study aimed to investigate the relationship between dietary practices and early childhood caries in a birth cohort of Australian preschoolers. Methods Participants underwent a standardized dental examination at 2–3 y of age to determine the prevalence of ECC (based on the presence of decayed, missing, or filled tooth surfaces). Breastfeeding practices were reported at 3, 6, 12, and 24 mo of age. Intakes of free sugars were assessed at 1 and 2 y of age. Multivariable regression models generated prevalence ratios (PR) for the association between ECC and breastfeeding duration, and between ECC and sleep feeding practices at 1 y, controlling for sociodemographic factors and free sugars intake. Results There was no independent association between breastfeeding beyond 1 y of age and ECC (PR 1.42, 95% CI: 0.85, 2.38), or between breastfeeding to sleep and ECC (PR 1.12, 95% CI: 0.67, 1.88), although the direction of effect was suggestive of an association. The only factors independently associated with ECC were high free sugars intakes (PR 1.97, 95% CI: 1.13, 3.44), and greater socioeconomic disadvantage (PR 2.15, 95% CI: 1.08, 4.28). Most participants who were breastfed at 1 y of age had ceased by 18 mo or 2 y. Conclusions Breastfeeding practices were not associated with ECC. Given the wide-ranging benefits of breastfeeding, and the low prevalence of sustained breastfeeding in this study and Australia in general, recommendations to limit breastfeeding are unwarranted, and breastfeeding should be promoted in line with global and national recommendations. To reduce the prevalence of early childhood caries, improved efforts are needed to limit foods high in free sugars.


2021 ◽  
Vol 12 ◽  
Author(s):  
Arumugam Priya ◽  
Anthonymuthu Selvaraj ◽  
Dass Divya ◽  
Ramalingam Karthik Raja ◽  
Shunmugiah Karutha Pandian

Early childhood caries (ECC), a severe form of caries due to cross-kingdom interaction of Candida albicans and Streptococcus mutans, is a serious childhood dental disease that affects majority of the children with poor background. The present study investigated the anti-infective potential of thymol against C. albicans and S. mutans dual species for the management of ECC. Thymol, a plant derivative of the monoterpene group, has been well known for its numerous biological activities. Thymol at 300 μg/ml concentration completely arrested growth and proliferation of dual species of C. albicans and S. mutans. Rapid killing efficacy of pathogens, within a span of 2 min, was observed in the time kill assay. In addition, at sub-inhibitory concentrations, thymol effectively diminished the biofilm formation and virulence of both C. albicans and S. mutans such as yeast-to-hyphal transition, hyphal-to-yeast transition, filamentation, and acidogenicity and acidurity, respectively, in single and dual species state. qPCR analysis was consistent with virulence assays. Also, through the invertebrate model system Galleria mellonella, in vivo toxicity and efficacy of the phytocompound was assessed, and it was found that no significant toxic effect was observed. Moreover, thymol was found to be proficient in diminishing the infection under single and dual state in in vivo condition. Overall, the results from the present study illustrate the anti-infective potential of thymol against the ECC-causing dual species, C. albicans and S. mutans, and the applicability of thymol in medicated dentifrice formulation.


2021 ◽  
Vol 14 (1) ◽  
pp. 1-7
Author(s):  
Muthiara Praziandite

Latar Belakang: Fussy Eating merupakan gangguan makan yang umum terjadi pada anak tetapi memilki dampak buruk bagi kesehatan, termasuk kesehatan gigi dan mulut. Early Childhood Caries (ECC) adalah penyakit jaringan keras gigi yang paling banyak terjadi pada anak dengan prevalensi mencapai 90% (Riskesdas, 2018) . Tujuan: peneltian ini bertujuan untuk mengetahui hubungan Fussy Eating dan Early Chidhhood Caries (ECC) pada anak.  Metode: penelitian ini merupakan penelitian observasional dengan desain cross sectional. Pengambilan sampel dilakukan purposive sampling dengan jumlah sampel sebanyak 165 orang berusia 3-5 tahun beserta ibunya. Children Eating Behaviour Qutionnaire (CEBQ) subskala Food Fussiness digunakan untuk mengukur Fussy Eating pada anak. ECC diukur dengan index def-t. Analisis data menggunakan uji chi-square. Hasil: Prevalensi anak dengan Fussy Eating adalah 50,9%. Terdapat hubungan bermakna antara Fussy Eating dan kejadian ECC pada anak (p-value = 0,010; OR = 2,380; 95% CI 1,273-4,450). Kesimpulan: Fussy Eating berkontribuasi dalam kejadian ECC.


Jurnal Surya ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 26-31
Author(s):  
Asyaul - Wasiah

Background : Dot, yang juga dikenal sebagai dummy, soother atau pacifier, adalah pengganti puting susu (ibu) yang biasanya terbuat dari karet atau plastik. Minum susu menggunakan dot menjelang tidur mulai menjadi kebiasaan yang sering kita jumpai. Padahal kebiasaan itu akan beresiko pada gigi anak. pada saat tidur kandungan gula dari minuman akan tersimpan dan menggenang lama di dalam mulut.  Dari situlah bakteri akan tumbuh subur pada lubang gigi.  Ditambah saat anak tidur air liur akan mengalami pengurangan. Jadi, genangan air minum mengandung gula tertahan oleh dot diantara lidah, gigi, langit-langit dan disekitar bibir . Hal seperti itulah gigi anak menjadi berlubang. Sindrom tersebut di dalam medis disebut nursing bottle caries atau Early Childhood CariesObjectivies : Tujuan penelitian ini untuk mengetahui Dampak Penggunan Dot Terhadap Sindrom Early Childhood Caries pada Anak usia 3-6 tahun di TK Nurul Huda Desa. Gedongboyountung Kec. Deket Kab. Lamongan.Design : Desain penelitian ini menggunakan metode penelitian deskriptif analitik dengan menggunakan rancangan cross sectional study ( study potong lintang) Populasinya adalah semua murid di TK Nurul Huda yaitu sebanyak 54 anak. Sampel dipilih secara Purposive Sampling. Analisa data terdiri dari a). Analisa Univariat untuk mengetahui distribusi frekuensi dari setiap variable independent, b) Analisa Bivariat untuk mengetahui hubungan antara variable independent. Dalam analisa ini dilakukan pengujian analitik dengan uji chi-square (α = 0.05).Result : Hasil uji chi-square test di atas didapatkan nilai signifikansi p-value sebesar 0.000. Karena nilai signifikansi 0.000 < (0.05) maka  H0 ditolak H1 diterima artinya ada Dampak Penggunan Dot Terhadap Sindrom Early Childhood Caries pada Anak usia 3-6 tahun di TK Nurul Huda Desa. Gedongboyountung Kec. Deket Kab. LamonganConclusion : Dapat menambah pengetahuan dan wawasan orangtua mengenai pola asuh orangtua yang tidak tepat dalam pemberian susu botol terhadap terjadinya early chilhood caries. Serta memberikan masukan agar diadakan program penyuluhan kesehatan rongga mulut di kalangan orangtua, anak dan guru sekolah agar langkah pencegahan terhadap karies bisa dilakukan sejak dini 


2020 ◽  
Author(s):  
Shanshan Liu ◽  
Huihui Li ◽  
Zhenfei Guo ◽  
Yu Sun ◽  
Qingwei Zheng ◽  
...  

Abstract The authors have withdrawn this preprint from Research Square.


2018 ◽  
Vol 2 (1) ◽  
pp. 51-59
Author(s):  
Gita Sjarkawi ◽  
Herry Novrinda ◽  
Armasastra Bahar

Cara pemberian makanan pada balita sedikit banyak dipengaruhi oleh tradisi budaya di suatu daerah tertentu. diantaranya adalah tradisi nasi papah atau seringkali juga disebut nasi papak yang masih banyak dilakukan oleh para ibu di beberapa wilayah di Indonesia, diantaranya di Kabupaten Lombok Timur, Propinsi Nusa Tenggara Barat. Tradisi nasi papah adalah nasi yang telah dikunyah dan dilumatkan terlebih dahulu sebelum diberikan kepada balita. Dari segi kesehatan terutama kesehatan mulut, hal ini berisiko terhadap terjadinya Early Childhood Caries(ECC). Perilaku tersebut dapat menyebabkan transmisi mikroorganisme S. mutans dari mulut ibu ke mulut anak. Tujuan Untuk mengetahui pengaruh tradisi nasi papah terhadap risiko terjadinya Early Childhood Caries. Desain yang digunakan adalah cross sectional dengan jumlah total sampel subyek penelitian sebanyak 186 anak berusia 6 – 60 bulan yang didampingi oleh ibunya, yang bertempat tinggal di Desa Senyiur, Kabupaten Lombok Timur, Nusa Tenggara Barat. Pemeriksaan Intra Oral dilakukan untuk mengukur karies gigi ibu dan anak dengan menggunakan indeks DMFT/deft dan untuk mendapatkan informasi mengenai perilaku kesehatan mulut ibu dan anak dilakukan wawancara pada ibu dengan menggunakan kuesioner. Data yang terkumpul dianalisa dengan uji Chi Square. perilaku nasi papah mempunyai risiko terhadap terjadinya ECC dengan OR 5,46 (95%CI 4,24-36,55), p


2020 ◽  
Vol 32 (3) ◽  
pp. 226
Author(s):  
Susi Susi ◽  
Rahmi Khairani Aulia ◽  
Murniwati Murniwati ◽  
Minarni Minarni

Pendahuluan: Early childhood caries (ECC) merupakan karies yang mengenai gigi sulung pada anak-anak dibawah usia 71 bulan. Pemerintah Indonesia menargetkan anak dibawah usia 12 tahun bebas karies pada tahun 2030. Tahun 2017 prevalensi ECC pada anak usia 2-3 tahun di Bukittinggi adalah 51.5%.Hal ini masih sangat jauh dari target pemerintah Indonesia. Pola minum susu dan pola makan merupakan faktor resiko karies. Tujuan penelitian adalah menganalisis hubungan antara pola minum susu  dengan early childhood caries. Metode: Jenis penelitian deskriptif dengan cross-sectional study di Posyandu Bukittinggi. Subyek dipilih secara multistage random sampling sebanyak 146 pasang ibu dan anak dengan rentang umur 2 sampai 5 tahun. Pola minum susu diobservasi menggunakan kuesioner pada ibu sedangkan ECC diperiksa secara visual. Data dikumpulkan dan dianalisa dengan chi square test dengan nilai kepercayaan 95%. Data diambil oleh enam orang dokter gigi muda pada Fakultas Kedokteran Gigi Universitas Andalas yang telah dikalibrasi sebelumnya. Data dianalisis menggunakan chi-square test. Hasil: Prevalensi ECC di Bukittinggi adalah 101 (69,2%) dengan indeks def-t rata-rata 3,00 ± SD 4,02 pada 95% CI (3,23 – 4,54). Sampel dengan ASI ekslusif 74 (50,7%) mempunyai indeks def-t lebih rendah dibanding non-ekslusif (3,70 vs. 4,07) dengan nilai p=0,678. Sampel dengan ASI dan makanan pendamping 68 (46,6%) mempunyai indeks def-t lebih rendah dibanding non ASI dan MP (3,51 vs. 4,21) dengan p=0,787. Simpulan: Secara statitik tidak terdapat pengaruh pola minum air susu ibu terhadap terjadinya early childhood caries pada anak di bawah usia lima tahun, namun kejadian early childhood caries lebih rendah pada pemberian ASI ekslusif dibandingkan dengan ASI non ekslusif.Kata kunci: ASI, early childhood caries, indeks def-t, makanan pendamping, prevalensi. ABSTRACTIntroduction: Early childhood caries (ECC) are caries that affect primary teeth in children under 71 months of age. The Indonesian government is targeting children under 12 years of age to be free from caries by 2030. In 2017, ECC prevalence in children aged 2-3 years in Bukittinggi was 51.5%. This data is still very far from the target of the Indonesian government. The pattern of milk consumption and dietary habit are caries risk factors. This research aimed to analyse the relationship between breast milk consumption pattern and early childhood caries. Methods: This was a descriptive study with a cross-sectional study at Bukittinggi Integrated Healthcare Centre (Posyandu). The subjects were selected by multistage random sampling, which obtained as many as 146 pairs of mothers and children aged 2 to 5 years. The breast milk consumption pattern was observed using a mother’s questionnaire while the ECC was examined visually. Data were collected and analysed using the chi-square test with a confidence value of 95%. Six young dentists took the data at the Faculty of Dentistry Andalas University who had been calibrated before. Data were analysed using the chi-square test. Results: The prevalence of ECC in Bukittinggi was 101 (69.2%) with the def-t index mean of 3.00 ± SD 4.02 at 95% CI (3.23 - 4.54). Samples with exclusive breastfeeding 74 (50.7%) had a lower def-t index than non-exclusive (3.70 vs 4.07) with the p-value=0.678. Samples with breast milk and complementary foods was 68 (46.6%), which showed a lower def-t index than non-breast milk and complementary food (3.51 vs 4.21) with p=0.787. Conclusion: Statistically, there is no effect of the pattern of breast milk consumption on the occurrence of early childhood caries in children under the age of five years, however, the incidence of early childhood caries is lower in exclusive breastfeed-ing children compared to non-exclusive breastfeeding.Keywords: Breast milk, early childhood caries, def-t index, complementary food, prevalence.


2018 ◽  
Vol 71 (3) ◽  
pp. 263-267
Author(s):  
Vidya Carolyn Tjokrosetio ◽  
Sarworini Bagio Budiardjo ◽  
Ike Siti Indiarti ◽  
Eva Fauziah ◽  
Margaretha Suharsini ◽  
...  

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