scholarly journals Near-Infrared Fluorescence Imaging of Carbonic Anhydrase IX in Athymic Mice Bearing HT-29 Tumor Xenografts

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jianbo Li ◽  
Baoliang Bao ◽  
Lei Liu ◽  
Xuemei Wang
Keyword(s):  
Near Infrared ◽  
Imaging Modality ◽  
Receptor Expression ◽  
Athymic Mice ◽  
Binding Studies ◽  
Preclinical Research ◽  
Near Infrared Fluorescence ◽  
Tumor Xenografts ◽  
Nirf Imaging ◽  
Ht 29

Near-infrared fluorescence (NIRF) imaging technology is a highly sensitive imaging modality and has been widely used in noninvasively studying the status of receptor expression in small animal models, with an appropriate NIRF probe targeting a specific receptor. In this report, Cy5.5-conjugated anti-CAIX monoclonal antibody (Mab-Cy5.5) was evaluated in athymic mice bearing HT-29 tumor xenografts in order to investigate the effect of conjugate on tumor targeting efficacy. In vitro binding studies showed that Mab-Cy5.5 could specifically bind to the cells which expressed CAIX. Results from in vivo imaging showed that HT-29 tumor xenografts can be clearly visualized at 48 h after injection of Mab-Cy5.5, and in the blocking experiment, free anti-CAIX antibody effectively blocked the concentration of Mab-Cy5.5 in the tumors. Western blotting and immunohistochemistry analysis of HT-29 tumor xenografts verified the expression of CAIX in HT-29 tumors. Mab-Cy5.5 could specifically bind to the tumors which expressed CAIX. These results suggested that Mab-Cy5.5 was suitable for CAIX expression imaging in the preclinical research.

scholarly journals Intravascular Molecular Imaging: Near-Infrared Fluorescence as a New Frontier

2020 ◽  
Vol 7 ◽  
Author(s):  
Haitham Khraishah ◽  
Farouc A. Jaffer
Keyword(s):  
Near Infrared ◽  
Imaging Modality ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Endothelial Permeability ◽  
Intravascular Imaging ◽  
Near Infrared Fluorescence ◽  
New Era ◽  
Nirf Imaging

Despite exciting advances in structural intravascular imaging [intravascular ultrasound (IVUS) and optical coherence tomography (OCT)] that have enabled partial assessment of atheroma burden and high-risk features associated with acute coronary syndromes, structural-based imaging modalities alone do not comprehensively phenotype the complex pathobiology of atherosclerosis. Near-infrared fluorescence (NIRF) is an emerging molecular intravascular imaging modality that allows for in vivo visualization of pathobiological and cellular processes at atheroma plaque level, including inflammation, oxidative stress, and abnormal endothelial permeability. Established intravascular NIRF imaging targets include macrophages, cathepsin protease activity, oxidized low-density lipoprotein and abnormal endothelial permeability. Structural and molecular intravascular imaging provide complementary information about plaque microstructure and biology. For this reason, integrated hybrid catheters that combine NIRF-IVUS or NIRF-OCT have been developed to allow co-registration of morphological and molecular processes with a single pullback, as performed for standalone IVUS or OCT. NIRF imaging is approaching application in clinical practice. This will be accelerated by the use of FDA-approved indocyanine green (ICG), which illuminates lipid- and macrophage-rich zones of permeable atheroma. The ability to comprehensively phenotype coronary pathobiology in patients will enable a deeper understanding of plaque pathobiology, improve local and patient-based risk prediction, and usher in a new era of personalized therapy.

scholarly journals Endoglin/CD105-Based Imaging of Cancer and Cardiovascular Diseases: A Systematic Review

2021 ◽  
Vol 22 (9) ◽  
pp. 4804
Author(s):  
Vincent Q. Sier ◽  
Joost R. van der Vorst ◽  
Paul H. A. Quax ◽  
Margreet R. de Vries ◽  
Elham Zonoobi ◽  
...  
Keyword(s):  
Systematic Review ◽  
Cardiovascular Diseases ◽  
Near Infrared ◽  
Transforming Growth Factor ◽  
Single Photon ◽  
Imaging Modality ◽  
Photon Emission ◽  
Emission Computed Tomography ◽  
Nirf Imaging ◽  
Efficient Detection

Molecular imaging of pathologic lesions can improve efficient detection of cancer and cardiovascular diseases. A shared pathophysiological feature is angiogenesis, the formation of new blood vessels. Endoglin (CD105) is a coreceptor for ligands of the Transforming Growth Factor-β (TGF-β) family and is highly expressed on angiogenic endothelial cells. Therefore, endoglin-based imaging has been explored to visualize lesions of the aforementioned diseases. This systematic review highlights the progress in endoglin-based imaging of cancer, atherosclerosis, myocardial infarction, and aortic aneurysm, focusing on positron emission tomography (PET), single-photon emission computed tomography (SPECT), magnetic resonance imaging (MRI), near-infrared fluorescence (NIRF) imaging, and ultrasound imaging. PubMed was searched combining the following subjects and their respective synonyms or relevant subterms: “Endoglin”, “Imaging/Image-guided surgery”. In total, 59 papers were found eligible to be included: 58 reporting about preclinical animal or in vitro models and one ex vivo study in human organs. In addition to exact data extraction of imaging modality type, tumor or cardiovascular disease model, and tracer (class), outcomes were described via a narrative synthesis. Collectively, the data identify endoglin as a suitable target for intraoperative and diagnostic imaging of the neovasculature in tumors, whereas for cardiovascular diseases, the evidence remains scarce but promising.

scholarly journals Intra-operative assessment of the vascularisation of a cross section of the meniscus using near-infrared fluorescence imaging

2021 ◽  
Author(s):  
Peter van Schie ◽  
Thies J. N. van der Lelij ◽  
Maxime Gerritsen ◽  
Ruben P. J. Meijer ◽  
Ewoud R. A. van Arkel ◽  
...  
Keyword(s):  
Total Knee Arthroplasty ◽  
Cross Section ◽  
Near Infrared ◽  
Level Of Evidence ◽  
Camera System ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
Total Knee ◽  
Operative Assessment ◽  
Near Infrared Fluorescence Imaging

Abstract Purpose The purpose of this study was to assess whether the vascularisation of the meniscus could be visualised intra-operatively using near-infrared fluorescence (NIRF) imaging with indocyanine green (ICG) in patients undergoing total knee arthroplasty (TKA). Methods The anterior horn (i.e., Cooper classification: zones C and D) of the meniscus that was least affected (i.e., least degenerative) was removed during TKA surgery in ten patients to obtain a cross section of the inside of the meniscus. Thereafter, 10 mg of ICG was injected intravenously, and vascularisation of the cross section of the meniscus was assessed using the Quest spectrum NIRF camera system. We calculated the percentage of patients in whom vascularisation was observed intra-operatively using NIRF imaging compared to immunohistochemistry. Results Meniscal vascularisation using NIRF imaging was observed in six out of eight (75%) patients in whom vascularisation was demonstrated with immunohistochemistry. The median extent of vascularisation was 13% (interquartile range (IQR) 3–28%) using NIRF imaging and 15% (IQR 11–23%) using immunohistochemistry. Conclusion This study shows the potential of NIRF imaging to visualise vascularisation of the meniscus, as vascularisation was observed in six out of eight patients with histologically proven meniscal vascularisation. Level of evidence IV.

scholarly journals Near Infrared Fluorescence Imaging to Assess Breast Flap Perfusion and Prevention of Ischemia: Case Report

2019 ◽  
Vol 103 (9-10) ◽  
pp. 473-476
Author(s):  
Ju Yong Cheong ◽  
David Goltsman ◽  
Sanjay Warrier
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Skin Flap ◽  
Color Temperature ◽  
Near Infrared Fluorescence ◽  
Clinical Method ◽  
Nirf Imaging ◽  
Near Infrared Fluorescence Imaging ◽  
Flap Perfusion

Introduction: Breast skin flap ischemia and necrosis is a serious consequence of breast reconstruction with reported incidence of 10% to 15%. Current clinical method of assessing breast skin flap perfusion includes combination of skin color, temperature, dermal edge bleeding, and capillary refill. Given the high incidence of ischemia and the presence of certain cohort of patient with greater risk, there is a need for an objective method of assessing the skin flap perfusion. Near infrared fluorescence imaging using indocyanine green is a new technique of assessing skin flap perfusion. We present a first reported case where breast skin flap ischemia was objectively identified intraoperatively by NIRF imaging, and this ischemia was reversed through active intra- and postoperative measures, which was objectively again identified by NIRF imaging. Case presentation: A 36-year-old female patient underwent bilateral nipple sparing mastectomies with immediate reconstruction using tissue expanders. Clinical assessment of the breast skin flap was equivocal. NIRF imaging using SPY imaging system (Novadaq Technologies Inc, Toronto, ON, Canada) showed poor perfusion in the periareolar region. As a result, it was decided to completely deflate the expander, actively warm the skin flap and encourage cutaneous perfusion through use of topical glycerin trinitrate patch. A repeat NIRF imaging 48 hours showed 10-fold increase in perfusion in the skin flap. Conclusion: NIRF imaging is a useful tool in assessing breast skin perfusion and to predict potential ischemia, which can aid in surgeon's management of patient.

scholarly journals Indocyanine Green Near-Infrared Fluorescence Imaging-Guided Laparoscopic Heminephrectomy for Left Ureteral Cancer in Patient with Horseshoe Kidney

2019 ◽  
Vol 2019 ◽  
pp. 1-5 ◽  
Author(s):  
Takao Natsuyama ◽  
Yozo Mitsui ◽  
Masato Uetani ◽  
Shigeyuki Ohta ◽  
Shin-ichi Hisasue
Keyword(s):  
Indocyanine Green ◽  
Near Infrared ◽  
Imaging System ◽  
Left Kidney ◽  
Horseshoe Kidney ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
Novel Approach ◽  
The Right ◽  
Near Infrared Fluorescence Imaging

Laparoscopic surgery for patients with a horseshoe kidney is challenging because of the location, aberrant vasculature, and difficulty with division of the isthmus with adequate hemostasis. We herein report performance of a laparoscopic heminephrectomy for left ureteral cancer in a patient with a horseshoe kidney under guidance from near-infrared fluorescence (NIRF) imaging using indocyanine green (ICG). A 62-year-old male was referred to our hospital for treatment of left ureteral cancer associated with a horseshoe kidney. We performed a laparoscopic left nephroureterectomy and bladder resection in June 2017. During the operation, the NIRF imaging system was used to evaluate the border of the kidney parenchyma isthmus after ligation of the left kidney vasculature supply. Interestingly, the dominant region of the right kidney showed strong ICG fluorescence as compared to the left kidney region. With the assistance of ICG-based NIRF imaging, isthmus division was performed with monopolar scissors and adequate hemostasis was obtained by electrocautery coagulation. This is the first report of use of an ICG-based NIRF imaging system and this novel approach can help to demarcate the left moiety isthmus from right one with more certainty.

scholarly journals In Vivo Near-Infrared Fluorescence Imaging of Matrix Metalloproteinase Activity after Cerebral Ischemia

2009 ◽  
Vol 29 (7) ◽  
pp. 1284-1292 ◽  
Author(s):  
Jan Klohs ◽  
Nevena Baeva ◽  
Jens Steinbrink ◽  
Riad Bourayou ◽  
Chotima Boettcher ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Near Infrared ◽  
Artery Occlusion ◽  
Substantial Contribution ◽  
Fluorescence Signal ◽  
Sham Operation ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
Activatable Probe

Matrix metalloproteinases (MMPs) have been implicated in the pathophysiology of cerebral ischemia. In this study, we explored whether MMP activity can be visualized by noninvasive near-infrared fluorescence (NIRF) imaging using an MMP-activatable probe in a mouse model of stroke. C57BI6 mice were subjected to transient middle cerebral artery occlusion (MCAO) or sham operation. Noninvasive NIRF imaging was performed 24 h after probe injection, and target-to-background ratios (TBRs) between the two hemispheres were determined. TBRs were significantly higher in MCAO mice injected with the MMP-activatable probe than in sham-operated mice and in MCAO mice that were injected with the nonactivatable probe as controls. Treatment with an MMP inhibitor resulted in significantly lower TBRs and lesion volumes compared to injection of vehicle. To test the contribution of MMP-9 to the fluorescence signal, MMP9-deficient (MMP9−/-) mice and wild-type controls were subjected to MCAO of different durations to attain comparable lesion volumes. TBRs were significantly lower in MMP9−/- mice, suggesting a substantial contribution of MMP-9 activity to the signal. Our study shows that MMP activity after cerebral ischemia can be imaged noninvasively with NIRF using an MMP-activatable probe, which might be a useful tool to study MMP activity in the pathophysiology of the disease.

In vivo pharmacodynamic evaluation of antidepressants based on flux mitochondrial Cys in living mice via near infrared fluorescence imaging

The Analyst ◽  
2020 ◽  
Vol 145 (18) ◽  
pp. 6119-6124
Author(s):  
Xin Wang ◽  
Jiali Zha ◽  
Wei Zhang ◽  
Wen Zhang ◽  
Bo Tang
Keyword(s):  
Fluorescence Imaging ◽  
Mouse Brain ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
New Strategy ◽  
Near Infrared Fluorescence Imaging

We proposed a new strategy for in vivo evaluation of antidepressants through NIRF imaging for mitochondrial Cys in the mouse brain.

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