scholarly journals In Vivo Near-Infrared Fluorescence Imaging of Matrix Metalloproteinase Activity after Cerebral Ischemia

2009 ◽  
Vol 29 (7) ◽  
pp. 1284-1292 ◽  
Author(s):  
Jan Klohs ◽  
Nevena Baeva ◽  
Jens Steinbrink ◽  
Riad Bourayou ◽  
Chotima Boettcher ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Near Infrared ◽  
Artery Occlusion ◽  
Substantial Contribution ◽  
Fluorescence Signal ◽  
Sham Operation ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
Activatable Probe

Matrix metalloproteinases (MMPs) have been implicated in the pathophysiology of cerebral ischemia. In this study, we explored whether MMP activity can be visualized by noninvasive near-infrared fluorescence (NIRF) imaging using an MMP-activatable probe in a mouse model of stroke. C57BI6 mice were subjected to transient middle cerebral artery occlusion (MCAO) or sham operation. Noninvasive NIRF imaging was performed 24 h after probe injection, and target-to-background ratios (TBRs) between the two hemispheres were determined. TBRs were significantly higher in MCAO mice injected with the MMP-activatable probe than in sham-operated mice and in MCAO mice that were injected with the nonactivatable probe as controls. Treatment with an MMP inhibitor resulted in significantly lower TBRs and lesion volumes compared to injection of vehicle. To test the contribution of MMP-9 to the fluorescence signal, MMP9-deficient (MMP9−/-) mice and wild-type controls were subjected to MCAO of different durations to attain comparable lesion volumes. TBRs were significantly lower in MMP9−/- mice, suggesting a substantial contribution of MMP-9 activity to the signal. Our study shows that MMP activity after cerebral ischemia can be imaged noninvasively with NIRF using an MMP-activatable probe, which might be a useful tool to study MMP activity in the pathophysiology of the disease.

scholarly journals Intravascular Molecular Imaging: Near-Infrared Fluorescence as a New Frontier

2020 ◽  
Vol 7 ◽  
Author(s):  
Haitham Khraishah ◽  
Farouc A. Jaffer
Keyword(s):  
Near Infrared ◽  
Imaging Modality ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Endothelial Permeability ◽  
Intravascular Imaging ◽  
Near Infrared Fluorescence ◽  
New Era ◽  
Nirf Imaging

Despite exciting advances in structural intravascular imaging [intravascular ultrasound (IVUS) and optical coherence tomography (OCT)] that have enabled partial assessment of atheroma burden and high-risk features associated with acute coronary syndromes, structural-based imaging modalities alone do not comprehensively phenotype the complex pathobiology of atherosclerosis. Near-infrared fluorescence (NIRF) is an emerging molecular intravascular imaging modality that allows for in vivo visualization of pathobiological and cellular processes at atheroma plaque level, including inflammation, oxidative stress, and abnormal endothelial permeability. Established intravascular NIRF imaging targets include macrophages, cathepsin protease activity, oxidized low-density lipoprotein and abnormal endothelial permeability. Structural and molecular intravascular imaging provide complementary information about plaque microstructure and biology. For this reason, integrated hybrid catheters that combine NIRF-IVUS or NIRF-OCT have been developed to allow co-registration of morphological and molecular processes with a single pullback, as performed for standalone IVUS or OCT. NIRF imaging is approaching application in clinical practice. This will be accelerated by the use of FDA-approved indocyanine green (ICG), which illuminates lipid- and macrophage-rich zones of permeable atheroma. The ability to comprehensively phenotype coronary pathobiology in patients will enable a deeper understanding of plaque pathobiology, improve local and patient-based risk prediction, and usher in a new era of personalized therapy.

In vivo pharmacodynamic evaluation of antidepressants based on flux mitochondrial Cys in living mice via near infrared fluorescence imaging

The Analyst ◽  
2020 ◽  
Vol 145 (18) ◽  
pp. 6119-6124
Author(s):  
Xin Wang ◽  
Jiali Zha ◽  
Wei Zhang ◽  
Wen Zhang ◽  
Bo Tang
Keyword(s):  
Fluorescence Imaging ◽  
Mouse Brain ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging ◽  
New Strategy ◽  
Near Infrared Fluorescence Imaging

We proposed a new strategy for in vivo evaluation of antidepressants through NIRF imaging for mitochondrial Cys in the mouse brain.

Novel Fluorophores as Building Blocks for Optical Probes for In Vivo Near Infrared Fluorescence (NIRF) Imaging

2010 ◽  
Vol 20 (3) ◽  
pp. 681-693 ◽  
Author(s):  
Jutta Pauli ◽  
Robert Brehm ◽  
Monika Spieles ◽  
Werner A. Kaiser ◽  
Ingrid Hilger ◽  
...  
Keyword(s):  
Near Infrared ◽  
Building Blocks ◽  
Optical Probes ◽  
Near Infrared Fluorescence ◽  
Nirf Imaging

A folate receptor-specific activatable probe for near-infrared fluorescence imaging of ovarian cancer

2014 ◽  
Vol 50 (56) ◽  
pp. 7507-7510 ◽  
Author(s):  
Hawon Lee ◽  
Jisu Kim ◽  
Hyunjin Kim ◽  
Youngmi Kim ◽  
Yongdoo Choi
Keyword(s):  
Ovarian Cancer ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Folate Receptor ◽  
Near Infrared Fluorescence ◽  
Background Ratio ◽  
Near Infrared Fluorescence Imaging ◽  
Activatable Probe

A folate receptor-specific activatable probe for in vivo near-infrared fluorescence imaging with a high target-to-background ratio was synthesized.

scholarly journals LINGO-1 shRNA protects the brain against ischemia/reperfusion injury by inhibiting the activation of NF-κB and JAK2/STAT3

Human Cell ◽  
2021 ◽  
Author(s):  
Jiaying Zhu ◽  
Zhu Zhu ◽  
Yipin Ren ◽  
Yukang Dong ◽  
Yaqi Li ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Nuclear Translocation ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Glucose Deprivation ◽  
Artery Occlusion ◽  
Mice Model ◽  
Down Regulation

AbstractLINGO-1 may be involved in the pathogenesis of cerebral ischemia. However, its biological function and underlying molecular mechanism in cerebral ischemia remain to be further defined. In our study, middle cerebral artery occlusion/reperfusion (MACO/R) mice model and HT22 cell oxygen–glucose deprivation/reperfusion (OGD/R) were established to simulate the pathological process of cerebral ischemia in vivo and in vitro and to detect the relevant mechanism. We found that LINGO-1 mRNA and protein were upregulated in mice and cell models. Down-regulation LINGO-1 improved the neurological symptoms and reduced pathological changes and the infarct size of the mice after MACO/R. In addition, LINGO-1 interference alleviated apoptosis and promoted cell proliferation in HT22 of OGD/R. Moreover, down-regulation of LINGO-1 proved to inhibit nuclear translocation of p-NF-κB and reduce the expression level of p-JAK2 and p-STAT3. In conclusion, our data suggest that shLINGO-1 attenuated ischemic injury by negatively regulating NF-KB and JAK2/STAT3 pathways, highlighting a novel therapeutic target for ischemic stroke.

A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging

Immunobiology ◽  
2015 ◽  
Vol 220 (12) ◽  
pp. 1328-1336 ◽  
Author(s):  
Hua He ◽  
Xiaojie Tu ◽  
Juan Zhang ◽  
Desmond Omane Acheampong ◽  
Li Ding ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Near Infrared Fluorescence ◽  
Antibody Targeting ◽  
Near Infrared Fluorescence Imaging

A near-infrared fluorescence off–on probe for sensitive imaging of hydrogen polysulfides in living cells and mice in vivo

2017 ◽  
Vol 53 (62) ◽  
pp. 8759-8762 ◽  
Author(s):  
Yu Fang ◽  
Wei Chen ◽  
Wen Shi ◽  
Hongyu Li ◽  
Ming Xian ◽  
...  
Keyword(s):  
Near Infrared ◽  
Living Cells ◽  
Near Infrared Fluorescence

A new near-infrared fluorescence off–on probe with phenyl 2-(benzoylthio)benzoate as the recognition moiety is developed and applied in imaging H2Sn in living cells and mice in vivo.

scholarly journals Micelle-based activatable probe for in vivo near-infrared optical imaging of cancer biomolecules

2014 ◽  
Vol 10 (1) ◽  
pp. 187-195 ◽  
Author(s):  
Yoichi Shimizu ◽  
Takashi Temma ◽  
Isao Hara ◽  
Akira Makino ◽  
Ryo Yamahara ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Activatable Probe

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

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