scholarly journals Production and Characterization of Polyhydroxyalkanoates and Native Microorganisms Synthesized from Fatty Waste

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Javier Ricardo Gómez Cardozo ◽  
Amanda Lucía Mora Martínez ◽  
María Yepes Pérez ◽  
Guillermo Antonio Correa Londoño
Keyword(s):  
Palm Oil ◽  
Substrate Concentration ◽  
Castor Oil ◽  
Statistical Design ◽  
Jatropha Oil ◽  
Previous Approach ◽  
Frying Oils ◽  
Native Strains ◽  
Product Costs

Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible plastics. They are synthesized by a wide variety of microorganisms (i.e., fungi and bacteria) and some organisms such as plants, which share characteristics with petrochemical-based plastics. The most recent studies focus on finding inexpensive substrates and extraction strategies that allow reducing product costs, thus moving into a widespread market, the market for petroleum-based plastics. In this study, the production of polyhydroxybutyrate (PHB) was evaluated using the native strains,Bacillus megaterium,Bacillussp., andLactococcus lactis, and glycerol reagent grade (GRG), residual glycerol (RGSB) byproduct of biodiesel from palm oil, Jatropha oil, castor oil, waste frying oils, and whey as substrates. Different bacteria-substrate systems were evaluated thrice on a laboratory scale under different conditions of temperature, pH, and substrate concentration, employing 50 mL of broth in 250 mL. The bacterial growth was tested in all systems; however, theB. megateriumGRG system generated the highest accumulation of PHA. The previous approach was allowed to propose a statistical design optimization with RGSB (i.e., RGSB, 15 g/L, pH 7.0, and 25°C). This system reached 2.80 g/L of PHB yield and was the optimal condition tested; however, the optimal biomass 5.42 g/L occurs at pH 9.0 and 25°C, with a substrate concentration of 22 g/L.

scholarly journals Synthesis and Characterization of Polyurethanes from Residual Palm Oil with High Poly-Unsaturated Fatty Acid Oils as Additive

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4214
Author(s):  
Javier Chavarro Gomez ◽  
Rabitah Zakaria ◽  
Min Min Aung ◽  
Mohd Noriznan Mokhtar ◽  
Robiah Yunus
Keyword(s):  
Palm Oil ◽  
Fire Retardant ◽  
Jatropha Oil ◽  
H Nmr ◽  
Fire Retardant Properties ◽  
Algae Oil ◽  
Oil Palm Industry ◽  
Poly Unsaturated Fatty Acid ◽  
Phosphate Groups

In the effort to produce renewable and biodegradable polymers, more studies are being undertaken to explore environmentally friendly sources to replace petroleum-based sources. The oil palm industry is not only the biggest vegetable-oil producer from crops but also one the biggest producers of residual oil that cannot be used for edible purposes due to its low quality. In this paper the development of biopolymers from residual palm oil, residual palm oil with 10% jatropha oil, and residual palm oil with 10% algae oil as additives were explored. Polyols from the different oils were prepared by epoxydation with peroxyacetic acid and alcoholysis under the same conditions and further reacted with poly isocyanate to form polyurethanes. Epoxidized oils, polyols and polyurethanes were analyzed by different techniques such as TGA, DSC, DMA, FTIR and H-NMR. Overall, although the IV of algae oil is slightly higher than that of jatropha oil, the usage of algae oil as additive into the residual palm oil was shown to significantly increase the hard segments and thermal stability of the bio polyurethane compared to the polymer with jatropha oil. Furthermore, when algae oil was mixed with the residual palm oil, it was possible to identify phosphate groups in the polyol which might enhance the fire-retardant properties of the final biopolymer.

scholarly journals Synthesis and Characterization of Novel Castor Oil-Based Polyol for Potential Applications in Coatings

2019 ◽  
Vol 7 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Azam Sardari ◽  
Ali Asghar Sabbagh Alvani ◽  
Seyed Reza Ghaffarian
Keyword(s):  
Castor Oil ◽  
Synthesis And Characterization ◽  
Potential Applications

Carbon footprint of renewable diesel from palm oil, jatropha oil and rapeseed oil

2014 ◽  
Vol 69 ◽  
pp. 103-113 ◽  
Author(s):  
V. Uusitalo ◽  
S. Väisänen ◽  
J. Havukainen ◽  
M. Havukainen ◽  
R. Soukka ◽  
...  
Keyword(s):  
Carbon Footprint ◽  
Palm Oil ◽  
Rapeseed Oil ◽  
Jatropha Oil ◽  
Renewable Diesel

scholarly journals Comparative Genomics and Characterization of the Late Promoter pR’ from Shiga Toxin Prophages in Escherichia coli

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 595 ◽  
Author(s):  
Ling Zhang ◽  
David Simpson ◽  
Lynn McMullen ◽  
Michael Gänzle
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Toxin Production ◽  
Late Promoter ◽  
Regulatory Systems ◽  
Human Illness ◽  
Late Transcription ◽  
Native Strains ◽  
Native Host

Shiga-toxin producing Escherichia coli (STEC) causes human illness ranging from mild diarrhea to death. The bacteriophage encoded stx genes are located in the late transcription region, downstream of the antiterminator Q. The transcription of the stx genes is directly under the control of the late promoter pR’, thus the sequence diversity of the region between Q and stx, here termed the pR’ region, may affect Stx toxin production. Here, we compared the gene structure of the pR’ region and the stx subtypes of nineteen STECs. The sequence alignment and phylogenetic analysis suggested that the pR’ region tends to be more heterogeneous than the promoter itself, even if the prophages harbor the same stx subtype. Furthermore, we established and validated transcriptional fusions of the pR’ region to the DsRed reporter gene using mitomycin C (MMC) induction. Finally, these constructs were transformed into native and non-native strains and examined with flow cytometry. The results showed that induction levels changed when pR’ regions were placed under different regulatory systems. Moreover, not every stx gene could be induced in its native host bacteria. In addition to the functional genes, the diversity of the pR’ region plays an important role in determining the level of toxin induction.

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