scholarly journals Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yufei Zhang ◽  
Jing Shi ◽  
Shuying Liu
Keyword(s):  
Cell Line ◽  
Soft Agar ◽  
Trophoblast Cell ◽  
Placental Lactogen ◽  
Trophoblast Cells ◽  
Human Telomerase Reverse Transcriptase ◽  
Htert Gene ◽  
Human Telomerase ◽  
Trophoblast Cell Line

The primary sheep trophoblast cells (STCs) have a finite lifespan in culture. This feature limits the scope for long-termin vitrostudies with STCs. This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line. STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively. The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs). hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence. Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunitβ(CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs. Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells. hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice. In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.

scholarly journals Targeting Epigenetic Modifiers Can Reduce the Clonogenic Capacities of Sézary Cells

2021 ◽  
Vol 11 ◽  
Author(s):  
Alain Chebly ◽  
Martina Prochazkova-Carlotti ◽  
Yamina Idrissi ◽  
Laurence Bresson-Bepoldin ◽  
Sandrine Poglio ◽  
...  
Keyword(s):  
Dna Methyltransferase ◽  
Histone Deacetylase Inhibitors ◽  
Soft Agar ◽  
Tumor Formation ◽  
Human Telomerase Reverse Transcriptase ◽  
Hematological Disorders ◽  
Soft Agar Assay ◽  
Human Telomerase ◽  
Sézary Cells

Sézary syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL) in which the human Telomerase Reverse Transcriptase (hTERT) gene is re-expressed. Current available treatments do not provide long-term response. We previously reported that Histone deacetylase inhibitors (HDACi, romidespin and vorinostat) and a DNA methyltransferase inhibitor (DNMTi, 5-azacytidine) can reduce hTERT expression without altering the methylation level of hTERT promoter. Romidepsin and vorinostat are approved for CTCL treatment, while 5-azacytidine is approved for the treatment of several hematological disorders, but not for CTCL. Here, using the soft agar assay, we analyzed the functional effect of the aforementioned epidrugs on the clonogenic capacities of Sézary cells. Our data revealed that, besides hTERT downregulation, epidrugs’ pressure reduced the proliferative and the tumor formation capacities in Sézary cells in vitro.

scholarly journals A Factor(s) from a Rat Trophoblast Cell Line Inhibits Prolactin Secretion in Vitro and in Vivo1

1993 ◽  
Vol 48 (2) ◽  
pp. 325-332 ◽  
Author(s):  
Hiroshi Tomogane ◽  
Lydia A. Arbogast ◽  
Michael J. Soares ◽  
May C. Robertson ◽  
James L. Voogt
Keyword(s):  
Cell Line ◽  
Prolactin Secretion ◽  
Trophoblast Cell ◽  
Trophoblast Cell Line

Establishment of Feeder-Independent Cloned Caprine Trophoblast Cell Line Which Expresses Placental Lactogen and Interferon Tau

Placenta ◽  
2002 ◽  
Vol 23 (8-9) ◽  
pp. 613-630 ◽  
Author(s):  
H. Miyazaki ◽  
M. Imai ◽  
T. Hirayama ◽  
S. Saburi ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Cell Line ◽  
Trophoblast Cell ◽  
Placental Lactogen ◽  
Interferon Tau ◽  
Trophoblast Cell Line

Cytotoxicity of Chronic Exposure to 4 Cigarette Smoke Condensates in 2 Cell Lines

2015 ◽  
Vol 34 (2) ◽  
pp. 182-194 ◽  
Author(s):  
Honggang Wang ◽  
Beverly Word ◽  
Lascelles Lyn-Cook ◽  
Maocheng Yang ◽  
George Hammons ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Line ◽  
Cigarette Smoke ◽  
Morphological Changes ◽  
Cell Damage ◽  
Airway Epithelial ◽  
Human Telomerase Reverse Transcriptase ◽  
Atp Assay ◽  
Human Telomerase

Tobacco use is the leading preventable cause of death. The cytotoxicity of cigarette smoke condensate (CSC), the particulate fraction of cigarette smoke without the vapor phase, has mostly been tested in short-term in vitro studies lasting from a few hours to a few days. Here, we assessed the toxicity of CSCs from 2 reference cigarettes, 3R4F and CM6, using a primary human small airway epithelial (PSAE) cell line by quantifying adenosine 5′-triphosphate (ATP), 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxy-methoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS), total glutathione (reduced glutathione [GSH] + oxidized glutathione [GSSG]), and lactate dehydrogenase (LDH) release over the course of 28 days. The CSCs, 0.3 to 10 μg/mL, promoted cell proliferation at 120 hours of exposure, but demonstrated cytotoxicity at days 14 and 28. Interestingly, CSCs, 0.3 to 3 μg/mL, showed a cell death effect at day 14 but induced cell proliferation at day 28. Consistently, transformation associated with morphological changes began by day 14 and the transformed cells grew dramatically at day 28. The LDH assay appeared to be sensitive for assessing early cell damage, whereas the ATP, MTS, and GSH assays were more suitable for determining later stage CSCs-induced cytotoxicity. The ATP assay showed greater sensitivity than the MTS and GSH assays. We also assessed the toxicity of CSCs in an human Telomerase Reverse Transcriptase (hTERT)-immortalized Barrett esophagus cell line (CP-C). The CP-C cells demonstrated dose- and time-dependent cytotoxicity over the course of 28 days but displayed higher resistance to CSCs than PSAE cells. This study demonstrates that CSCs cause cytotoxicity and induce transformation related to cell resistance and cell invasion properties.

scholarly journals Limited replication of human cytomegalovirus in a trophoblast cell line

2021 ◽  
Vol 102 (11) ◽  
Author(s):  
Kadeem Hyde ◽  
Nowshin Sultana ◽  
Andy C. Tran ◽  
Narina Bileckaja ◽  
Claire L. Donald ◽  
...  
Keyword(s):  
Cell Line ◽  
Vertical Transmission ◽  
Human Cytomegalovirus ◽  
Molecular Mechanisms ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Cell Protein ◽  
Trophoblast Cells ◽  
Hcmv Replication ◽  
Trophoblast Cell Line

Several viruses, including human cytomegalovirus (HCMV), are thought to replicate in the placenta. However, there is little understanding of the molecular mechanisms involved in HCMV replication in this tissue. We investigated replication of HCMV in the extravillous trophoblast cell line SGHPL-4, a commonly used model of HCMV replication in the placenta. We found limited HCMV protein expression and virus replication in SGHPL-4 cells. This was associated with a lack of trophoblast progenitor cell protein markers in SGHPL-4 cells, suggesting a relationship between trophoblast differentiation and limited HCMV replication. We proposed that limited HCMV replication in trophoblast cells is advantageous to vertical transmission of HCMV, as there is a greater opportunity for vertical transmission when the placenta is intact and functional. Furthermore, when we investigated the replication of other vertically transmitted viruses in SGHPL-4 cells we found some limitation to replication of Zika virus, but not herpes simplex virus. Thus, limited replication of some, but not all, vertically transmitted viruses may be a feature of trophoblast cells.

scholarly journals Effect of steroids on transcription and secretion of Gal-1 by the human trophoblast cell line in vitro

2013 ◽  
Vol 65 (4) ◽  
pp. 1331-1337 ◽  
Author(s):  
Danica Cujic ◽  
Zanka Bojic-Trbojevic ◽  
Natasa Tosic ◽  
Sonja Pavlovic ◽  
Ljiljana Vicovac
Keyword(s):  
Cell Line ◽  
Culture Media ◽  
Mrna Level ◽  
Trophoblast Cell ◽  
Mrna Levels ◽  
Human Trophoblast ◽  
Present Evidence ◽  
Trophoblast Cell Line ◽  
Synthetic Glucocorticoid

Galectin-1 (Gal-1) is a lectin with recently documented pro-invasive function in trophoblasts in vitro, whose regulation is currently insufficiently known. The potential involvement of steroid hormones, synthetic glucocorticoid dexamethasone (DEX), the sex steroid progesterone (PRG) and mifepristone (RU486) in the regulation of Gal-1 in the trophoblast-derived cell line HTR-8/SVneo was investigated. Gal-1 mRNA levels were assessed by real-time PCR. The effect on secretion of Gal-1 into the culture media was followed using the SELDI-TOF protein chip array. We present evidence that DEX and RU486 significantly reduced Gal-1 in the HTR-8/SVneo cell line at the mRNA level. In addition, trophoblast-derived HTR-8/SVneo cells were shown to secrete detectable Gal-1 protein, which was only slightly increased by PRG. The potential clinical relevance of these findings remains to be determined.

scholarly journals The effect of IL-6 on the trophoblast cell line HTR-8/SVneo

2010 ◽  
Vol 62 (3) ◽  
pp. 531-538 ◽  
Author(s):  
Milica Jovanovic ◽  
Tamara Kovacevic ◽  
Ivana Stefanoska ◽  
Ljiljana Vicovac
Keyword(s):  
Cell Line ◽  
Normal Pregnancy ◽  
Blastocyst Stage ◽  
Uterine Wall ◽  
Trophoblast Cell ◽  
Trophoblast Invasion ◽  
Invasion And Migration ◽  
And Migration ◽  
Trophoblast Cell Line

Embryonic development up to the blastocyst stage, implantation into the uterine wall and the development of the functional placenta are steps crucial for the establishment of normal pregnancy. Specific cells of the placenta, the trophoblast cells, invade the uterine stroma and spiral arteries, adapting them to pregnancy. Interleukin-6 is present in the human endometrium during the receptive phase and early pregnancy. Trophoblasts also produce IL-6, which was found to stimulate trophoblast invasion and migration in vitro. Here we show that the activity of MMP-9 may contribute to the observed increased invasion. In addition, in the HTR-8/SVneo trophoblast cell line IL-6 increases cell proliferation. .

scholarly journals Gamma Interferon Fails To Induce Expression of Indoleamine 2,3-Dioxygenase and Does Not Control the Growth of Chlamydophila abortus in BeWo Trophoblast Cells

2002 ◽  
Vol 70 (5) ◽  
pp. 2690-2693 ◽  
Author(s):  
Gary Entrican ◽  
Sean Wattegedera ◽  
Michelle Chui ◽  
Lyn Oemar ◽  
Mara Rocchi ◽  
...  
Keyword(s):  
Cell Line ◽  
Hela Cells ◽  
Gamma Interferon ◽  
Trophoblast Cell ◽  
Trophoblast Cells ◽  
Degrading Enzyme ◽  
Chlamydophila Abortus ◽  
Bewo Cells ◽  
Indoleamine 2,3 Dioxygenase ◽  
Trophoblast Cell Line

ABSTRACT The BeWo trophoblast cell line does not constitutively express the tryptophan degrading enzyme indolamine 2,3-dioxygenase (IDO), nor can IDO expression be induced by gamma interferon. This correlates with the inability of BeWo cells to control the growth of Chlamydophila abortus, in contrast to effects observed in HeLa cells treated with gamma interferon.

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