Establishment of Feeder-Independent Cloned Caprine Trophoblast Cell Line Which Expresses Placental Lactogen and Interferon Tau

Placenta ◽  
2002 ◽  
Vol 23 (8-9) ◽  
pp. 613-630 ◽  
Author(s):  
H. Miyazaki ◽  
M. Imai ◽  
T. Hirayama ◽  
S. Saburi ◽  
M. Tanaka ◽  
...  
Keyword(s):  
Cell Line ◽  
Trophoblast Cell ◽  
Placental Lactogen ◽  
Interferon Tau ◽  
Trophoblast Cell Line

scholarly journals Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yufei Zhang ◽  
Jing Shi ◽  
Shuying Liu
Keyword(s):  
Cell Line ◽  
Soft Agar ◽  
Trophoblast Cell ◽  
Placental Lactogen ◽  
Trophoblast Cells ◽  
Human Telomerase Reverse Transcriptase ◽  
Htert Gene ◽  
Human Telomerase ◽  
Trophoblast Cell Line

The primary sheep trophoblast cells (STCs) have a finite lifespan in culture. This feature limits the scope for long-termin vitrostudies with STCs. This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line. STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively. The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs). hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence. Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunitβ(CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs. Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells. hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice. In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.

Interferon Tau Regulates Cytokine Production and Cellular Function in Human Trophoblast Cell Line

2017 ◽  
Vol 37 (10) ◽  
pp. 456-466 ◽  
Author(s):  
Nao Tanikawa ◽  
Kotomi Seno ◽  
Ryouka Kawahara-Miki ◽  
Koji Kimura ◽  
Shuichi Matsuyama ◽  
...  
Keyword(s):  
Cell Line ◽  
Cytokine Production ◽  
Cellular Function ◽  
Trophoblast Cell ◽  
Interferon Tau ◽  
Human Trophoblast ◽  
Trophoblast Cell Line

scholarly journals MAML1: a coregulator that alters endometrial epithelial cell adhesive capacity

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Sadaf Zafir ◽  
Wei Zhou ◽  
Ellen Menkhorst ◽  
Leilani Santos ◽  
Evdokia Dimitriadis
Keyword(s):  
Notch Signaling ◽  
Cell Line ◽  
Notch Signaling Pathway ◽  
Endometrial Receptivity ◽  
Trophoblast Cell ◽  
Luminal Epithelium ◽  
Secretory Phase ◽  
Ishikawa Cells ◽  
Trophoblast Cell Line ◽  
Adhesive Capacity

Abstract Background Abnormalities in endometrial receptivity has been identified as a major barrier to successful embryo implantation. Endometrial receptivity refers to the conformational and biochemical changes occurring in the endometrial epithelial layer which make it adhesive and receptive to blastocyst attachment. This takes place during the mid-secretory phase of woman’s menstrual cycle and is a result of a delicate interplay between numerous hormones, cytokines and other factors. Outside of this window, the endometrium is refractory to an implanting blastocyst. It has been shown that Notch ligands and receptors are dysregulated in the endometrium of infertile women. Mastermind Like Transcriptional Coactivator 1 (MAML1) is a known coactivator of the Notch signaling pathway. This study aimed to determine the role of MAML1 in regulating endometrial receptivity. Methods The expression and localization of MAML1 in the fertile human endometrium (non-receptive proliferative phase versus receptive mid-secretory phase) were determined by immunohistochemistry. Ishikawa cells were used as an endometrial epithelial model to investigate the functional consequences of MAML1 knockdown on endometrial adhesive capacity to HTR8/SVneo (trophoblast cell line) spheroids. After MAML1 knockdown in Ishikawa cells, the expression of endometrial receptivity markers and Notch dependent and independent pathway members were assessed by qPCR. Two-tailed unpaired or paired student’s t-test were used for statistical analysis with a significance threshold of P < 0.05. Results MAML1 was localized in the luminal epithelium, glandular epithelium and stroma of human endometrium and the increased expression identified in the mid-secretory phase was restricted only to the luminal epithelium (P < 0.05). Functional analysis using Ishikawa cells demonstrated that knockdown of MAML1 significantly reduced epithelial adhesive capacity (P < 0.01) to HTR8/SVneo (trophoblast cell line) spheroids compared to control. MAML1 knockdown significantly affected the expression of classical receptivity markers (SPP1, DPP4) and this response was not directly via hormone receptors. The expression level of Hippo pathway target Ankyrin repeat domain-containing protein 1 (ANKRD1) was also affected after MAML1 knockdown in Ishikawa cells. Conclusion Our data strongly suggest that MAML1 is involved in regulating the endometrial adhesive capacity and may facilitate embryo attachment, either directly or indirectly through the Notch signaling pathway.

Caspase 8 inhibition increases the infection with Trypanosoma cruzi in the human trophoblast cell line (BeWo)

Placenta ◽  
2016 ◽  
Vol 45 ◽  
pp. 95
Author(s):  
Ileana Carrillo ◽  
Christian Castillo ◽  
Daniel Droguett ◽  
Lorena Muñoz ◽  
Ana Liempi ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Cell Line ◽  
Trophoblast Cell ◽  
Caspase 8 ◽  
Human Trophoblast ◽  
Trophoblast Cell Line

Impact of human first trimester trophoblast cell line ACH-3P on the function of endothelial cells under different oxygen concentrations

Placenta ◽  
2013 ◽  
Vol 34 (9) ◽  
pp. A47
Author(s):  
Gregor Weiss ◽  
Ingrid Lang ◽  
Monika Siwetz ◽  
Berthold Huppertz ◽  
Gerit Moser
Keyword(s):  
Endothelial Cells ◽  
Cell Line ◽  
First Trimester ◽  
Trophoblast Cell ◽  
Trophoblast Cell Line ◽  
Oxygen Concentrations

H+-linked transport of salicylic acid, an NSAID, in the human trophoblast cell line BeWo

2002 ◽  
Vol 282 (5) ◽  
pp. C1064-C1075 ◽  
Author(s):  
Akiko Emoto ◽  
Fumihiko Ushigome ◽  
Noriko Koyabu ◽  
Hiroshi Kajiya ◽  
Koji Okabe ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Lactic Acid ◽  
Cell Line ◽  
Intracellular Ph ◽  
Trophoblast Cell ◽  
Monocarboxylate Transporter ◽  
Human Trophoblast ◽  
Inflammatory Drugs ◽  
Ph Dependent ◽  
Trophoblast Cell Line

We investigated the transport of salicylic acid and l-lactic acid across the placenta using the human trophoblast cell line BeWo. We performed uptake experiments and measured the change in intracellular pH (pHi). The uptakes of [14C]salicylic acid andl-[14C]lactic acid were temperature- and extracellular pH-dependent and saturable at higher concentrations. Both uptakes were also reduced by FCCP, nigericin, and NaN3. Various nonsteroidal anti-inflammatory drugs (NSAIDs) strongly inhibited the uptake of l-[14C]lactic acid. Salicylic acid and ibuprofen noncompetitively inhibited the uptake ofl-[14C]lactic acid. α-Cyano-4-hydroxycinnamate (CHC), a monocarboxylate transporter inhibitor, suppressed the uptake ofl-[14C]lactic acid but not that of [14C]salicylic acid. CHC also suppressed the decrease of pHi induced by l-lactic acid but had little effect on that induced by salicylic acid or diclofenac. These results suggest that NSAIDs are potent inhibitors of lactate transporters, although they are transported mainly by a transport system distinct from that for l-lactic acid.

Partial characterization of an immortalized human trophoblast cell-line, TCL-1, which possesses a CSF-1 autocrine loop

Placenta ◽  
1996 ◽  
Vol 17 (2-3) ◽  
pp. 137-146 ◽  
Author(s):  
M.P. Lewis ◽  
M. Clements ◽  
S. Takeda ◽  
P.L. Kirby ◽  
H. Seki ◽  
...  
Keyword(s):  
Cell Line ◽  
Trophoblast Cell ◽  
Partial Characterization ◽  
Human Trophoblast ◽  
Autocrine Loop ◽  
Trophoblast Cell Line
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