scholarly journals TheGinkgo bilobaExtract Reverses the Renal Effects of Titanium Dioxide Nanoparticles in Adult Male Rats

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Carlos Enrique Escárcega-González ◽  
Irma Guadalupe Reynoso-Andeola ◽  
Fernando Jaramillo-Juárez ◽  
Haydée Martínez-Ruvalcaba ◽  
Francisco A. Posadas del Rio
Keyword(s):  
Titanium Dioxide ◽  
Herbal Remedy ◽  
Titanium Dioxide Nanoparticles ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules ◽  
Male Adult ◽  
Cytoplasmic Membranes ◽  
Dipeptidylaminopeptidase Iv ◽  
Increased Activity

TheGinkgo bilobaextract (GbE) is a commercial product used as a nutraceutic herbal remedy in Europe and US. It contains 27% of the polyphenols isorhamnetin, kaempferol, and quercetin, as antioxidants. We used male adult Wistar rats (200–300 g), divided into four groups: control group (treated with 5.0 mg/kg of sodium chloride, intravenous), titanium dioxide nanoparticles (TiO2-NPs) group (5.0 mg/kg, intravenous), GbE group (10 mg/kg, intraperitoneal), and GbE + TiO2-NPs group (treated 24 h before with 10 mg/kg of GbE, intraperitoneal), followed, 24 h later, by 5.0 mg/kg of TiO2-NPs intravenously. The statistical analysis was performed using Student’st-test for grouped data with ANOVA posttest. The GbE protected renal cells against the effects of TiO2-NPs because it reversed the increased activity ofγ-glutamyltranspeptidase and the enzymatic activity of dipeptidylaminopeptidase IV at all times tested (0–5, 5–24, 24–48, and 48–72 h). Also it reversed the glucosuria, hypernatriuria, and urine osmolarity at three times tested (5–24, 24–48, and 48–72). Thus, we conclude that GbE has a beneficial activity in the cytoplasmic membranes of brush border cells on the renal tubules, against the adverse effects that can be produced by some xenobiotics in this case the TiO2-NPs, in experimental rats.

scholarly journals Effects of Titanium Dioxide Nanoparticles Toxicity on the Kidney of Male Rats

2016 ◽  
Vol 10 (1) ◽  
pp. 65-69 ◽  
Author(s):  
Fatemeh Mohammadi Fartkhooni ◽  
Ali Noori ◽  
Abdolrasool Mohammadi
Keyword(s):  
Titanium Dioxide ◽  
Uric Acid ◽  
Titanium Dioxide Nanoparticles ◽  
Life Sciences ◽  
Kidney Tissue ◽  
Male Rats ◽  
Control Group ◽  
Biological And Medical Applications ◽  
Bowman's Capsule ◽  
Nanoparticles Toxicity

Nanoparticles have many characteristics that make them suitable for biological and medical applications. These special features cause some adverse effects. Despite the widespread use of titanium dioxide nanoparticles, few studies were conducted on the toxicity of these nanoparticles in biological systems. In this study, the effects of intraperitoneal injections of TiO2 nanoparticles (30,50,70 mg/kg ) were investigated on kidney function and histology. The creatinine levels did not change but the amount of urea and uric acid showed the significant changes in comparison with control group. But, by passing the time, the uric acid changes back to normal. In kidney tissue, the changes such as deposition of hyaline-like materials, the swelling, dilatation of Bowman's capsule and degenerations were seen. It seems by passing time, The temporary disorders have been removed and the renal function has returned toward normal. However, further investigations are needed to measure the oxidative stress.International Journal of Life Sciences 10 (1) : 2016; 65-69

scholarly journals Effect of Titanium Dioxide Nanoparticles on the Histology of Liver of White Male Rats

2020 ◽  
Vol 10 (03) ◽  
pp. 468-471
Author(s):  
Firas F. Almamori ◽  
Fakhir M. Alzubaidy ◽  
Raad Jasim ◽  
Dhulfiqar Ali Abed
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
White Male ◽  
Inflammatory Cells ◽  
Study Groups ◽  
Male Rats ◽  
Control Group ◽  
Lymphocyte Infiltration ◽  
Group I ◽  
Effective Role

Background: Nanotechnology or nanoscience considers as a new field of science which has an effective role in the day to day life aspects. This field deals with the production, processing, and use of materials ranging in nanometers. Recently, this field becomes a confederate science that is usually used in other fields of science, like electronics, physics, and engineering. Materials and methods: Study design starts with the choice of nanoparticles (NPs) [titanium dioxide nanoparticles (TiO2)]. Eighteen white male rats were used in this research. Study groups classified into six groups (each group includes three animals): two control groups (for oral and i.p. administration) and four treatment groups (group I: treated with 400 mg/kg orally and i.p., group II: treated with 150 mg/kg orally and i.p.). Results and discussion: Results of this research include the presence of some inflammatory cells infiltration (lymphocyte infiltration), as it showed in the figures of the histological sections of the treated groups compared with the control group. These results were found to be different than studies of other researches that refer to the presence of many undesired hepatic histological and biochemical changes, such as, the effects on membrane structure, oxidative stress, binding protein, or DNA, cell death or apoptosis, and hepatocytes necrosis. Conclusion: In conclusion, Tio2 induce lymphocyte infiltration, but there are no additional deleterious histological changes in the liver tissue.

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

scholarly journals The protective role of CsNPs and CurNPs against DNA damage, oxidative stress, and histopathological and immunohistochemical alterations induced by hydroxyapatite nanoparticles in male rat kidney

2019 ◽  
Vol 8 (5) ◽  
pp. 741-753 ◽  
Author(s):  
Israa F. Mosa ◽  
Mokhtar I. Yousef ◽  
Maher Kamel ◽  
Osama F. Mosa ◽  
Yasser Helmy
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Enzymes ◽  
Biological Effects ◽  
Male Rats ◽  
Nuclear Antigen ◽  
Male Rat ◽  
Control Group ◽  
Renal Tubules ◽  
Hydroxyapatite Nanoparticles

Abstract Hydroxyapatite nanoparticles (HAP-NPs) are an inorganic component of natural bone and are mainly used in the tissue engineering field due to their bioactivity, osteoconductivity, biocompatibility, non-inflammatory, and non-toxicity properties. However, the current toxicity data for HAP-NPs regarding human health are limited, and only a few results from basic studies have been published. Therefore, the present study was designed to investigate the beneficial role of chitosan nanoparticles (CsNPs) and curcumin nanoparticles (CurNPs) in alleviating nephrotoxicity induced by HAP-NPs in male rats. The results showed that HAP-NPs caused a reduction in antioxidant enzymes and induced lipid peroxidation, nitric oxide production and DNA oxidation. Moreover, HAP-NP administration was associated with intense histologic changes in kidney architecture and immunoreactivity to proliferating cell nuclear antigen (PCNA). However, the presence of CsNPs and/or CurNPs along with HAP-NPs reduced the levels of oxidative stress through improving the activities of antioxidant enzymes. Also, the rats administered the nanoparticles showed a moderate improvement in glomerular damage which matched that of the control group and showed mild positive reactions to PCNA–ir in glomeruli and renal tubules in the cortical and medullary portions. These novel insights confirm that the presence of chitosan and curcumin in nanoforms has powerful biological effects with enhanced bioactivity and bioavailability phenomena compared to their microphase counterparts. Also, they were able to ameliorate the nephrotoxicity induced by HAP-NPs.

scholarly journals Evaluation of the Transverse Strength of the Heat Cure PMMA Resin Reinforced with Various Concentrations of Two Different Nanoparticles: An In vitro Study

2019 ◽  
pp. 1-8
Author(s):  
Kusha Thakur ◽  
Archana Nagpal ◽  
Rajeev Gupta ◽  
Ramit Verma ◽  
Rohit Saini ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Testing Machine ◽  
Bending Test ◽  
Control Group ◽  
Transverse Strength ◽  
Heat Cure ◽  
Group 3 ◽  
Post Hoc ◽  
Pmma Resin

Purpose: The purpose of the study was to evaluate the effect of titanium dioxide and zirconia nanoparticles on transverse strength of heat cure PMMA resin routinely used for complete denture fabrication. Methods: One hundred samples of PMMA resin were made and divided into five groups (20 samples for each group). The test specimens were divided into five groups depending on the concentration of reinforcing nanoparticles as Group 1,2,3,4 and 5; Group 1: PMMA unreinforced (control group), Group 2: PMMA reinforced with 2.5% nanozirconia, Group 3: PMMA reinforced with 5% nanozirconia, Group 4: PMMA reinforced with 2.5% titanium dioxide nanoparticles, and Group 5: PMMA reinforced with 5% titanium dioxide nanoparticles. Universal testing machine was used to conduct a three-point bending test and evaluate the transverse strength of samples. Comparison of mean transverse strength for various groups was carried out by employing one‑way analysis of variance and Bonferroni post hoc tests. Results: The highest and lowest mean transverse strength were observed in Group 3 and 1, respectively. Bonferroni post hoc test showed increase in transverse strength after reinforcement to be statistically significant between all the groups (P = .05) except between the samples of group G1 and G5 and G2 and G3. Conclusion: Addition of nanoparticles in all concentrations significantly increased transverse strength of heat cure PMMA resin as compared to control group. The best result was obtained after adding 5% of nanozirconia particles to the conventional heat polymerized acrylic resin.

scholarly journals Histological Effects of Titanium Dioxide Nanoparticles Size 10 Nm in Mice Testes

2017 ◽  
Vol 5 (2) ◽  
pp. 158 ◽  
Author(s):  
Imad Taher Abdulla
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Testicular Tissue ◽  
Germinal Epithelium ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Histological Changes ◽  
Low Concentrations ◽  
High Concentrations ◽  
Histological Effect

In the present study, the histological effect of titanium dioxide nanoparticles (TDN) on testicular tissue of mature Mus musculus mice was investigated. The animals were divided into six groups, control group treated with TDN free saline and five groups treated with TDN as follow 5, 10, 50, 100 and 150 mg/kg B.W. The results showed that TDN has histological effects on testicular tissue like sever congestion, mild edema between seminiferous tubules (STs) and decrease the thickness of germinal epithelium at low concentrations, While, the histological changes at high concentrations involved disturbance in STs diameters, sever edema between STs, sever vaculation in the germinal epithelium and necrosis in spermatogonia, germinal epithelium and Sertoli cells.

Analysis of the preventive effect of Ginkgo biloba on renal effects of titanium dioxide nanoparticles in adult male rats

2014 ◽  
Vol 229 ◽  
pp. S190
Author(s):  
Carlos Enrique Escarcega-Gonzalez ◽  
Maria Luisa Rodriguez-Vazquez ◽  
Fernando Jaramillo-Juarez ◽  
Eduardo De La Cerda-Gonzalez ◽  
Haydee Martinez-Ruvalcaba ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Adult Male ◽  
Ginkgo Biloba ◽  
Titanium Dioxide Nanoparticles ◽  
Male Rats ◽  
Preventive Effect ◽  
Renal Effects

Biodistribution and Toxicity Study of Titanium Dioxide Nanoparticles of Different Sizes after Intravenous Injection in Mice

2014 ◽  
Vol 998-999 ◽  
pp. 196-199
Author(s):  
Jie Fang ◽  
Lu Lu Yuan ◽  
Chen Jie Yao ◽  
Yan Li Wang ◽  
Ming Hong Wu
Keyword(s):  
Titanium Dioxide ◽  
Biochemical Parameters ◽  
Titanium Dioxide Nanoparticles ◽  
Control Group ◽  
Quantitative Distribution ◽  
Blood Biochemical ◽  
Long Time ◽  
Liver And Kidney ◽  
Kidney Functions

Titanium dioxide nanoparticles (TiO2-NPs) are widely used in many fields. The bio-distribution and toxicology of different sizes (25 nm and 50 nm) of TiO2-NPsin vivois evaluated in this paper. The results of quantitative distributionin vivoshow that it is difficult for TiO2-NPs to excretion clearly from tissues and organs. At the endpoint of experiment of different groups, there are still many TiO2-NPs located in the main organs such as spleen, liver and lung after 120 days. Blood biochemical parameters keep unchanged compared with control group. However, pathological results show that lesions in spleen, liver and lung where TiO2-NPs particles are mainly accumulated and retained are getting serious with the time prolonging. The results indicate that TiO2-NPs are low toxic to mice but may have some potential effects on liver and kidney functions after exposure long time. The fundamental importance information gained from such a study will give the guidance on the application of TiO2-NPs.

Tiron ameliorates oxidative stress and inflammation in titanium dioxide nanoparticles induced nephrotoxicity of male rats

2017 ◽  
Vol 93 ◽  
pp. 779-787 ◽  
Author(s):  
Ashraf Morgan ◽  
Mona K. Galal ◽  
Hanan A. Ogaly ◽  
Marwa A. Ibrahim ◽  
Reham M. Abd-Elsalam ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Male Rats

Ce(IV) Doped TiO2 Nanoparticles and the Effect on Proliferation of BEL7402 Human Hepatoma Cells

2007 ◽  
Vol 330-332 ◽  
pp. 307-310
Author(s):  
L. Wang ◽  
J. Mao ◽  
X.L. Zhang ◽  
M.J. Tu ◽  
B. Kan ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Titanium Dioxide Nanoparticles ◽  
Hepatoma Cells ◽  
Control Group ◽  
Impregnation Method ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Transmission Electron ◽  
Induced Apoptosis

To investigate nanoparticles’ antitumor effect, a globose 30~35nm Ce(IV) doped Titanium dioxide nanoparticles (CDT) were prepared by impregnation method and characterized by X-ray diffraction (XRD) and transmission electron microscopy(TEM). Proliferation of BEL7402 human Hepatoma cells was studied in vitro by using fluorescence microscopy and Flow Cytometry. The results show that cerium elements doping enhanced thermal stability of nano-size titanium dioxide to 800°C. With UV irradiated for 8min, BEL7402 were induced a dose-dependent apoptosis by CDT, about 28.2% , 41.5% and 88.3% cells were induced apoptosis after 24h at concentration of 60, 120 and 180ug/ml respectively, relative to 3.9% of that of control group cell just only in the presence of UV.

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