scholarly journals Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models

2016 ◽  
Vol 2016 ◽  
pp. 1-25 ◽  
Author(s):  
Mariáurea Matias Sarandy ◽  
Fernanda Barbosa Lopes ◽  
Sérgio Luis Pinto da Matta ◽  
Marcus Vinicius Mello Pinto ◽  
Sirlene Souza Rodrigues Sartori ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Herbal Medicines ◽  
Tissue Expansion ◽  
Topical Administration ◽  
Experimental Models ◽  
Skin Wound ◽  
Current Evidence ◽  
Closure Rate ◽  
Skin Wound Healing

Background and Purpose.Skin wound healing is a dynamic process driven by molecular events responsible for the morphofunctional repair of the injured tissue. In a systematic review, we analyzed the relevance of plant fractions and isolates on skin wound healing. By revising preclinical investigations with murine models, we investigated if the current evidence could support clinical trials.Methods.Studies were selected in the MEDLINE/PubMed and Scopus databases according to the PRISMA statement. All 32 identified studies were submitted to data extraction and the methodological bias was investigated according to ARRIVE strategy.Results.The studies demonstrated that plant fractions and isolates are able to modulate the inflammatory process during skin wound healing, being also effective in attenuating the oxidative tissue damage in the scar tissue and stimulating cell proliferation, neoangiogenesis, collagen synthesis, granulation tissue expansion, reepithelialization, and the wound closure rate. However, we identified serious methodological flaws in all studies, such as the high level of reporting bias and absence of standardized experimental designs, analytical methods, and outcome measures.Conclusion.Considering these limitations, the current evidence generated from flawed methodological animal studies makes it difficult to determine the relevance of herbal medicines to treat skin wounds and derails conducting clinical studies.

Collagen from Marine Sources and Skin Wound Healing in Animal Experimental Studies: a Systematic Review

2021 ◽  
Author(s):  
Matheus Almeida Cruz ◽  
Tiago Akira Araujo ◽  
Ingrid Regina Avanzi ◽  
Julia Risso Parisi ◽  
Ana Laura Martins de Andrade ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Experimental Studies ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Systemic and topical administration of spermidine accelerates skin wound healing

2020 ◽  
Author(s):  
Daisuke Ito ◽  
Hiroyasu Ito ◽  
Takayasu Ideta ◽  
Ayumu Kanbe ◽  
Soranobu Ninomiya ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Topical Administration ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing

Abstract Background The skin wound healing process is regulated by various cytokines, chemokines, and growth factors. Recent reports have demonstrated that spermine/spermidine (SPD) promote wound healing through urokinase-type plasminogen activator (uPA)/uPA receptor (uPAR) signaling in vitro. Here, we investigated whether the systemic and topical administration of SPD would accelerate the skin wound-repair process in vivo.Methods A skin wound repair model was established using C57BL/6 J mice. SPD was mixed with white petrolatum for topical administration. For systemic administration, SPD mixed with drinking water was orally administered. Changes in wound size over time were calculated using digital photography.Results Systemic and topical SPD treatment significantly accelerated skin wound healing. The administration of SPD promoted the uPA/uPAR pathway in wound sites. Moreover, topical treatment with SPD enhanced the expression of IL-6 and TNF-α in wound sites. Scratch and cell proliferation assays revealed that SPD administration accelerated scratch wound closure and cell proliferation in vitro.Conclusion These results indicate that treatment with SPD promotes skin wound healing through activation of the uPA/uPAR pathway and induction of the inflammatory response in wound sites. The administration of SPD might contribute to new effective treatments to accelerate skin wound healing.

scholarly journals Peptides from Animal Origin: A Systematic Review on Biological Sources and Effects on Skin Wounds

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Raul Santos Alves ◽  
Levy Bueno Alves ◽  
Luciana Schulthais Altoé ◽  
Mariáurea Matias Sarandy ◽  
Mariella Bontempo Freitas ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Methodological Quality ◽  
Cellular Proliferation ◽  
Opportunistic Infections ◽  
Skin Wound ◽  
Animal Origin ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
The Impact

Background. Skin wounds are closely correlated with opportunistic infections and sepsis risk. Due to the need of more efficient healing drugs, animal peptides are emerging as new molecular platforms to accelerate skin wound closure and to prevent and control bacterial infection. Aim. The aim of this study was to evaluate the preclinical evidence on the impact of animal peptides on skin wound healing. In addition, we carried out a critical analysis of the studies’ methodological quality. Main Methods. This systematic review was performed according to the PRISMA guidelines, using a structured search on the PubMed-Medline, Scopus, and Web of Science platforms to retrieve studies published until August 25, 2020 at 3 : 00 pm. The studies included were limited to those that used animal models, investigated the effect of animal peptides with no association with other compounds on wound healing, and that were published in English. Bias analysis and methodological quality assessments were examined through the SYRCLE’s RoB tool. Results. Thirty studies were identified using the PRISMA workflow. In general, animal peptides were effective in accelerating skin wound healing, especially by increasing cellular proliferation, neoangiogenesis, colagenogenesis, and reepithelialization. Considering standardized methodological quality indicators, we identified a marked heterogeneity in research protocols and a high risk of bias associated with limited characterization of the experimental designs. Conclusion. Animal peptides show a remarkable healing potential with biotechnological relevance for regenerative medicine. However, rigorous experimental approaches are still required to clearly delimit the mechanisms underlying the healing effects and the risk-benefit ratio attributed to peptide-based treatments.

scholarly journals Antimicrobial photodynamic therapy in skin wound healing: A systematic review of animal studies

2019 ◽  
Vol 17 (2) ◽  
pp. 285-299 ◽  
Author(s):  
Yan Sun ◽  
Rei Ogawa ◽  
Bi‐Huan Xiao ◽  
Yu‐Xin Feng ◽  
Yan Wu ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Animal Studies ◽  
Skin Wound ◽  
Antimicrobial Photodynamic Therapy ◽  
Skin Wound Healing

Collagen membranes for skin wound repair: A systematic review

2020 ◽  
pp. 088532822098027
Author(s):  
Tiago Akira Tashiro Araujo ◽  
Matheus Cruz Almeida ◽  
Ingrid Avanzi ◽  
Julia Parisi ◽  
Abdias Fernando Simon Sales ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Healing Process ◽  
Biological Tissues ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Efficient Treatment ◽  
Positive Effects ◽  
Collagen Membranes

Membranes or skin dressing are common treatments for skin wound injuries, collagen being one the most effective materials for their manufacturing. Many different sources of collagen with diverse methods of extraction and processing have been used, with evidence of positive effects on the stimulation of skin wound healing. In spite of these factors, there is still limited understanding of the interaction between collagen membranes and biological tissues, especially due to the series of different types of collagen origin. In this context, this study aimed to conduct a systematic review of the available literature examining the effect of various collagen membranes for accelerating skin wound healing in experimental animal models and clinical trials. The present review was performed from March to May of 2020 searching in two databases (PubMed and Scopus). The following Medical Subject Headings (MeSH) descriptors were used: “collagen”, “dressing”, “membranes”, “skin” and “wound”. After the eligibility assessment, 16 studies were included and analyzed. The studies demonstrated that collagen was obtained predominantly from bovine and porcine sources, by acetic acid and/or enzyme dissolution. Additionally, most of the studies demonstrated that the membranes were processed mainly by freeze-drying or lyophilization methods. All the in vivo and clinical trial studies evidenced positive outcomes in the wound healing process, thus confirming that collagen membranes are one of the most efficient treatment for skin wounds, highlighting the enormous potential of this biomaterial to be used for skin tissue engineering purposes.

Wound healing effect benzosulfonate 1-ethyl-3-methyl-4,5-bis(n-methylcarbamoyl) imidazolium

2020 ◽  
Vol 18 (3) ◽  
pp. 229-235
Author(s):  
Lydmila K. Khnychenko ◽  
Elena N. Selina ◽  
Olga M. Rodionova ◽  
Levon B. Piotrovskiy ◽  
Petr D. Shabanov
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Experimental Models ◽  
Skin Wound ◽  
Complete Healing ◽  
Male Rats ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
Healing Effect ◽  
Wound Healing Effect

Materials and methods. In experiments on 180 male rats weighing 180200 g, the wound-healing effect of derivatives of imidazole (IEM-1181) was evaluated as a 10% ointment on models of aseptic full-layer linear and planar skin wounds. Results. It was found that the compound IEM-1181 in the form of 10% ointment has a pronounced on skin wound healing effect, manifested in the qualitative features of the regenerative healing process. With local application of the ointment containing the tested compound, the strength of the tissue formed on the site of a full-layer linear skin wound was increased when its edges were stretched, and the healing time of full-layer planar wounds was reduced. The morphological picture of the tissue formed at the site of the wound defect corresponded to complete healing by primary tension with complete epithelization of the surface and squamous cell differentiation of the epithelial regenerate without signs of inflammation and scar formation. Conclusion. The results of the study on experimental models of full-layer linear and planar skin wounds indicate that the wound healing effect of the tested ointment is due to the anti-inflammatory activity of the IEM-1181 compound

scholarly journals The Immune Functions of Keratinocytes in Skin Wound Healing

2020 ◽  
Vol 21 (22) ◽  
pp. 8790
Author(s):  
Minna Piipponen ◽  
Dongqing Li ◽  
Ning Xu Landén
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Chronic Wound ◽  
Skin Wound ◽  
Current Evidence ◽  
Immune Functions ◽  
Skin Wound Healing ◽  
Protein Coding ◽  
Chromatin Dynamics ◽  
Skin Keratinocytes

As the most dominant cell type in the skin, keratinocytes play critical roles in wound repair not only as structural cells but also exerting important immune functions. This review focuses on the communications between keratinocytes and immune cells in wound healing, which are mediated by various cytokines, chemokines, and extracellular vesicles. Keratinocytes can also directly interact with T cells via antigen presentation. Moreover, keratinocytes produce antimicrobial peptides that can directly kill the invading pathogens and contribute to wound repair in many aspects. We also reviewed the epigenetic mechanisms known to regulate keratinocyte immune functions, including histone modifications, non-protein-coding RNAs (e.g., microRNAs, and long noncoding RNAs), and chromatin dynamics. Lastly, we summarized the current evidence on the dysregulated immune functions of keratinocytes in chronic nonhealing wounds. Based on their crucial immune functions in skin wound healing, we propose that keratinocytes significantly contribute to the pathogenesis of chronic wound inflammation. We hope this review will trigger an interest in investigating the immune roles of keratinocytes in chronic wound pathology, which may open up new avenues for developing innovative wound treatments.

scholarly journals Topical Administration of Heat-Killed Enterococcus faecalis Strain KH2 Promotes Re-Epithelialization and Granulation Tissue Formation during Skin Wound-Healing

Biomedicines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1520
Author(s):  
Hiromasa Tanno ◽  
Emi Kanno ◽  
Shiho Kurosaka ◽  
Yukari Oikawa ◽  
Takumi Watanabe ◽  
...  
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Therapeutic Potential ◽  
Topical Administration ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Beneficial Effects ◽  
Growth Factor Production ◽  
Chronic Skin ◽  
Tgf Β1

Lactic acid bacteria (LAB) are known to have beneficial effects on immune responses when they are orally administered as bacterial products. Although the beneficial effects of LAB have been reported for the genera Lactobacillus and Lactococcus, little has been uncovered on the effects of the genus Enterococcus on skin wound-healing. In this study, we aimed to clarify the effect of heat-killed Enterococcus faecalis KH2 (heat-killed KH2) strain on the wound-healing process and to evaluate the therapeutic potential in chronic skin wounds. We analyzed percent wound closure, re-epithelialization, and granulation area, and cytokine and growth factor production. We found that heat-killed KH2 contributed to the acceleration of re-epithelialization and the formation of granulation tissue by inducing tumor necrosis factor-α, interleukin-6, basic fibroblast growth factor, transforming growth factor (TGF)-β1, and vascular endothelial growth factor production. In addition, heat-killed KH2 also improved wound closure, which was accompanied by the increased production of TGF-β1 in diabetic mice. Topical administration of heat-killed KH2 might have therapeutic potential for the treatment of chronic skin wounds in diabetes mellitus. In the present study, we concluded that heat-killed KH2 promoted skin wound-healing through the formation of granulation tissues and the production of inflammatory cytokines and growth factors.

scholarly journals Experimental Models to Study Skin Wound Healing with a Focus on Angiogenesis

2021 ◽  
Vol 9 (3) ◽  
pp. 55
Author(s):  
Eberhard Grambow ◽  
Heiko Sorg ◽  
Christian G. G. Sorg ◽  
Daniel Strüder
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Vascular Supply ◽  
Experimental Models ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Pathological Conditions ◽  
Different Levels ◽  
Crucial Parameter

A large number of models are now available for the investigation of skin wound healing. These can be used to study the processes that take place in a phase-specific manner under both physiological and pathological conditions. Most models focus on wound closure, which is a crucial parameter for wound healing. However, vascular supply plays an equally important role and corresponding models for selective or parallel investigation of microcirculation regeneration and angiogenesis are also described. In this review article, we therefore focus on the different levels of investigation of skin wound healing (in vivo to in virtuo) and the investigation of angiogenesis and its parameters.

scholarly journals Systemic and topical administration of spermidine accelerates skin wound healing

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Daisuke Ito ◽  
Hiroyasu Ito ◽  
Takayasu Ideta ◽  
Ayumu Kanbe ◽  
Soranobu Ninomiya ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Topical Administration ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing

Abstract Background The skin wound healing process is regulated by various cytokines, chemokines, and growth factors. Recent reports have demonstrated that spermine/spermidine (SPD) promote wound healing through urokinase-type plasminogen activator (uPA)/uPA receptor (uPAR) signaling in vitro. Here, we investigated whether the systemic and topical administration of SPD would accelerate the skin wound-repair process in vivo. Methods A skin wound repair model was established using C57BL/6 J mice. SPD was mixed with white petrolatum for topical administration. For systemic administration, SPD mixed with drinking water was orally administered. Changes in wound size over time were calculated using digital photography. Results Systemic and topical SPD treatment significantly accelerated skin wound healing. The administration of SPD promoted the uPA/uPAR pathway in wound sites. Moreover, topical treatment with SPD enhanced the expression of IL-6 and TNF-α in wound sites. Scratch and cell proliferation assays revealed that SPD administration accelerated scratch wound closure and cell proliferation in vitro. Conclusion These results indicate that treatment with SPD promotes skin wound healing through activation of the uPA/uPAR pathway and induction of the inflammatory response in wound sites. The administration of SPD might contribute to new effective treatments to accelerate skin wound healing.

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