scholarly journals Antimicrobial photodynamic therapy in skin wound healing: A systematic review of animal studies

2019 ◽  
Vol 17 (2) ◽  
pp. 285-299 ◽  
Author(s):  
Yan Sun ◽  
Rei Ogawa ◽  
Bi‐Huan Xiao ◽  
Yu‐Xin Feng ◽  
Yan Wu ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Animal Studies ◽  
Skin Wound ◽  
Antimicrobial Photodynamic Therapy ◽  
Skin Wound Healing

Collagen from Marine Sources and Skin Wound Healing in Animal Experimental Studies: a Systematic Review

2021 ◽  
Author(s):  
Matheus Almeida Cruz ◽  
Tiago Akira Araujo ◽  
Ingrid Regina Avanzi ◽  
Julia Risso Parisi ◽  
Ana Laura Martins de Andrade ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Experimental Studies ◽  
Skin Wound ◽  
Skin Wound Healing

scholarly journals Peptides from Animal Origin: A Systematic Review on Biological Sources and Effects on Skin Wounds

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Raul Santos Alves ◽  
Levy Bueno Alves ◽  
Luciana Schulthais Altoé ◽  
Mariáurea Matias Sarandy ◽  
Mariella Bontempo Freitas ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Methodological Quality ◽  
Cellular Proliferation ◽  
Opportunistic Infections ◽  
Skin Wound ◽  
Animal Origin ◽  
Skin Wounds ◽  
Skin Wound Healing ◽  
The Impact

Background. Skin wounds are closely correlated with opportunistic infections and sepsis risk. Due to the need of more efficient healing drugs, animal peptides are emerging as new molecular platforms to accelerate skin wound closure and to prevent and control bacterial infection. Aim. The aim of this study was to evaluate the preclinical evidence on the impact of animal peptides on skin wound healing. In addition, we carried out a critical analysis of the studies’ methodological quality. Main Methods. This systematic review was performed according to the PRISMA guidelines, using a structured search on the PubMed-Medline, Scopus, and Web of Science platforms to retrieve studies published until August 25, 2020 at 3 : 00 pm. The studies included were limited to those that used animal models, investigated the effect of animal peptides with no association with other compounds on wound healing, and that were published in English. Bias analysis and methodological quality assessments were examined through the SYRCLE’s RoB tool. Results. Thirty studies were identified using the PRISMA workflow. In general, animal peptides were effective in accelerating skin wound healing, especially by increasing cellular proliferation, neoangiogenesis, colagenogenesis, and reepithelialization. Considering standardized methodological quality indicators, we identified a marked heterogeneity in research protocols and a high risk of bias associated with limited characterization of the experimental designs. Conclusion. Animal peptides show a remarkable healing potential with biotechnological relevance for regenerative medicine. However, rigorous experimental approaches are still required to clearly delimit the mechanisms underlying the healing effects and the risk-benefit ratio attributed to peptide-based treatments.

Collagen membranes for skin wound repair: A systematic review

2020 ◽  
pp. 088532822098027
Author(s):  
Tiago Akira Tashiro Araujo ◽  
Matheus Cruz Almeida ◽  
Ingrid Avanzi ◽  
Julia Parisi ◽  
Abdias Fernando Simon Sales ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Healing Process ◽  
Biological Tissues ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing ◽  
Efficient Treatment ◽  
Positive Effects ◽  
Collagen Membranes

Membranes or skin dressing are common treatments for skin wound injuries, collagen being one the most effective materials for their manufacturing. Many different sources of collagen with diverse methods of extraction and processing have been used, with evidence of positive effects on the stimulation of skin wound healing. In spite of these factors, there is still limited understanding of the interaction between collagen membranes and biological tissues, especially due to the series of different types of collagen origin. In this context, this study aimed to conduct a systematic review of the available literature examining the effect of various collagen membranes for accelerating skin wound healing in experimental animal models and clinical trials. The present review was performed from March to May of 2020 searching in two databases (PubMed and Scopus). The following Medical Subject Headings (MeSH) descriptors were used: “collagen”, “dressing”, “membranes”, “skin” and “wound”. After the eligibility assessment, 16 studies were included and analyzed. The studies demonstrated that collagen was obtained predominantly from bovine and porcine sources, by acetic acid and/or enzyme dissolution. Additionally, most of the studies demonstrated that the membranes were processed mainly by freeze-drying or lyophilization methods. All the in vivo and clinical trial studies evidenced positive outcomes in the wound healing process, thus confirming that collagen membranes are one of the most efficient treatment for skin wounds, highlighting the enormous potential of this biomaterial to be used for skin tissue engineering purposes.

Scarless wound repair: a human fetal skin model

Development ◽  
1992 ◽  
Vol 114 (1) ◽  
pp. 253-259 ◽  
Author(s):  
H.P. Lorenz ◽  
M.T. Longaker ◽  
L.A. Perkocha ◽  
R.W. Jennings ◽  
M.R. Harrison ◽  
...  
Keyword(s):  
Wound Healing ◽  
Gestational Age ◽  
Animal Studies ◽  
Wound Repair ◽  
Mouse Skin ◽  
Skin Wound ◽  
Scar Formation ◽  
Fetal Skin ◽  
Skin Wound Healing ◽  
Thickness Skin

Animal studies demonstrate that the fetus heals cutaneous wounds by reformation of normal tissue architecture without scar formation. We have developed a new model to study human fetal skin wound healing. Grafts of human fetal skin placed onto athymic mice retain the morphologic features of normal development, although they differentiate at an accelerated rate when placed cutaneously compared to subcutaneously. Full-thickness skin grafts from human fetuses at 15 (n = 12), 17 (n = 11), 18 (n = 25), 19 (n = 20) and 22 (n = 13) weeks gestational age were placed onto athymic (nu/nu) mice in 2 locations: (1) cutaneously onto a fascial bed and thereby exposed to air or (2) subcutaneously in a pocket under the murine panniculus carnosus. Linear incisions were made in each graft 7 days after transplantation. Grafts were harvested at 7, 14 and 21 days postwounding and analyzed histologically for scar formation. By hematoxylin & eosin and Mallory's trichrome stains, complete epidermal and dermal graft wound healing without scar formation was demonstrated in the subcutaneous grafts at each gestational age studied. In contrast, scar was seen at all time points in the cutaneous grafts in both the incisional wound and at the interface of the fetal human skin graft and adult mouse skin, regardless of fetal skin gestational age.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization

2021 ◽  
Vol 9 ◽  
Author(s):  
Zengjun Yang ◽  
Xiaohong Hu ◽  
Lina Zhou ◽  
Yaxiong He ◽  
Xiaorong Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Wound Healing ◽  
Photodynamic Therapy ◽  
Skin Wound ◽  
Hair Follicles ◽  
Skin Wound Healing ◽  
And Migration ◽  
Proliferation And Migration

Abstract Background Epidermal stem cells (EpSCs) that reside in cutaneous hair follicles and the basal layer of the epidermis are indispensable for wound healing and skin homeostasis. Little is known about the effects of photochemical activation on EpSC differentiation, proliferation and migration during wound healing. The present study aimed to determine the effects of photodynamic therapy (PDT) on wound healing in vivo and in vitro. Methods We created mouse full-thickness skin resection models and applied 5-aminolevulinic acid (ALA) for PDT to the wound beds. Wound healing was analysed by gross evaluation and haematoxylin–eosin staining in vivo. In cultured EpSCs, protein expression was measured using flow cytometry and immunohistochemistry. Cell migration was examined using a scratch model; apoptosis and differentiation were measured using flow cytometry. Results PDT accelerated wound closure by enhancing EpSC differentiation, proliferation and migration, thereby promoting re-epithelialization and angiogenesis. PDT inhibited inflammatory infiltration and expression of proinflammatory cytokines, whereas the secretion of growth factors was greater than in other groups. The proportion of transient amplifying cells was significantly greater in vivo and in vitro in the PDT groups. EpSC migration was markedly enhanced after ALA-induced PDT. Conclusions Topical ALA-induced PDT stimulates wound healing by enhancing re-epithelialization, promoting angiogenesis as well as modulating skin homeostasis. This work provides a preliminary theoretical foundation for the clinical administration of topical ALA-induced PDT in skin wound healing.

scholarly journals Effect of Topical Administration of Fractions and Isolated Molecules from Plant Extracts on Skin Wound Healing: A Systematic Review of Murine Experimental Models

2016 ◽  
Vol 2016 ◽  
pp. 1-25 ◽  
Author(s):  
Mariáurea Matias Sarandy ◽  
Fernanda Barbosa Lopes ◽  
Sérgio Luis Pinto da Matta ◽  
Marcus Vinicius Mello Pinto ◽  
Sirlene Souza Rodrigues Sartori ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Herbal Medicines ◽  
Tissue Expansion ◽  
Topical Administration ◽  
Experimental Models ◽  
Skin Wound ◽  
Current Evidence ◽  
Closure Rate ◽  
Skin Wound Healing

Background and Purpose.Skin wound healing is a dynamic process driven by molecular events responsible for the morphofunctional repair of the injured tissue. In a systematic review, we analyzed the relevance of plant fractions and isolates on skin wound healing. By revising preclinical investigations with murine models, we investigated if the current evidence could support clinical trials.Methods.Studies were selected in the MEDLINE/PubMed and Scopus databases according to the PRISMA statement. All 32 identified studies were submitted to data extraction and the methodological bias was investigated according to ARRIVE strategy.Results.The studies demonstrated that plant fractions and isolates are able to modulate the inflammatory process during skin wound healing, being also effective in attenuating the oxidative tissue damage in the scar tissue and stimulating cell proliferation, neoangiogenesis, collagen synthesis, granulation tissue expansion, reepithelialization, and the wound closure rate. However, we identified serious methodological flaws in all studies, such as the high level of reporting bias and absence of standardized experimental designs, analytical methods, and outcome measures.Conclusion.Considering these limitations, the current evidence generated from flawed methodological animal studies makes it difficult to determine the relevance of herbal medicines to treat skin wounds and derails conducting clinical studies.

scholarly journals Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

2019 ◽  
Vol 20 (15) ◽  
pp. 3679 ◽  
Author(s):  
Lin Chen ◽  
Alyne Simões ◽  
Zujian Chen ◽  
Yan Zhao ◽  
Xinming Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Oral Mucosa ◽  
Wound Closure ◽  
Expression Patterns ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Specific Expression ◽  
Keratinocyte Proliferation

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.

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