scholarly journals Vegetable Contamination by the Fecal Bacteria of Poultry Manure: Case Study of Gardening Sites in Southern Benin

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Séraphin C. Atidégla ◽  
Joël Huat ◽  
Euloge K. Agbossou ◽  
Hervé Saint-Macary ◽  
Romain Glèlè Kakai
Keyword(s):  
Escherichia Coli ◽  
Poultry Manure ◽  
Fecal Coliforms ◽  
Fecal Bacteria ◽  
Vegetable Crops ◽  
Traditional Use ◽  
Fresh Vegetables ◽  
Fecal Streptococci ◽  
Cropping Seasons ◽  
Southern Benin

A study was conducted in southern Benin to assess the contamination of vegetables by fecal coliforms,Escherichia coli, and fecal streptococci as one consequence of the intensification of vegetable cropping through fertilization with poultry manure. For this purpose, on-farm trials were conducted in 2009 and 2010 at Yodo-Condji and Ayi-Guinnou with three replications and four fertilization treatments including poultry manure and three vegetable crops (leafy eggplant, tomato, and carrot). Sampling, laboratory analyses, and counts of fecal bacteria in the samples were performed in different cropping seasons. Whatever the fertilization treatment, the logs of mean fecal bacteria count per g of fresh vegetables were variable but higher than AFNOR criteria. The counts ranged from 8 to 10 fecal coliforms, from 5 to 8 fecal streptococci, and from 2 to 6Escherichia coli, whereas AFNOR criteria are, respectively, 0, 1, and 0. The long traditional use of poultry manure and its use during the study helped obtain this high population of fecal pathogens. Results confirmed that the contamination of vegetables by fecal bacteria is mainly due to the use of poultry manure. The use of properly composted poultry manure with innovative cropping techniques should help reduce the number and incidence of pathogens.

Presence of Cryptosporidium Oocysts in Fresh Vegetables

1996 ◽  
Vol 59 (2) ◽  
pp. 202-203 ◽  
Author(s):  
RAFAEL MONGE ◽  
MISAEL CHINCHILLA
Keyword(s):  
Escherichia Coli ◽  
Linear Correlation ◽  
Rainy Season ◽  
Fecal Coliforms ◽  
Contamination Rate ◽  
E Coli ◽  
Cryptosporidium Spp ◽  
Fresh Vegetables ◽  
Cryptosporidium Oocysts ◽  
Positive Linear Correlation

In Costa Rica, a total of 640 samples from eight different vegetables used for raw consumption were analyzed for the presence of Cryptosporidium spp. oocysts, fecal coliforms, and Escherichia coli. Cryptosporidium spp. oocysts were found in 5.0% (4 samples) of cilantro leaves, 8.7% (7 samples) of cilantro roots and 2.5% (2 samples) of lettuce samples. A 1.2% contamination rate was detected in samples of other vegetables (radish, tomato, cucumbers and carrot). Oocysts of this parasite were absent from cabbage. A greater percentage of positive samples was found during the rainy season, and only in cilantro roots and lettuce was a positive linear correlation (P < 0.05) established between the presence of Cryptosporidium spp. oocysts and fecal coliforms and E. coli.

Microflora Isolated from Imported Frozen Lean Beef Pieces1

1978 ◽  
Vol 41 (4) ◽  
pp. 251-253 ◽  
Author(s):  
J. L. OBLINGER ◽  
J. E. KENNEDY
Keyword(s):  
Escherichia Coli ◽  
Clostridium Perfringens ◽  
Microbial Flora ◽  
Fecal Coliforms ◽  
Meat Processing ◽  
Microbial Counts ◽  
Lean Beef ◽  
Fecal Streptococci ◽  
Yeasts And Molds

Forty samples of frozen imported lean beef pieces from six countries were obtained from two centralized meat processing operations. The samples were analyzed for total aerobic counts (35, 20, 7 C), yeasts and molds, fecal streptococci, Staphylococcus aureus, Clostridium perfringens, total coliforms, fecal coliforms, Escherichia coli, and Salmonella. Characterization of the microbial flora from 20 of the samples was also done. Microbial counts were consistently low in all analyses; no Clostridium perfringens or Salmonella was recovered from any samples. The microbial flora was predominantly Pseudomonas, Flavobacterium, Moraxella, Acinetobacter, Corynebacterium, Micrococcus, Staphylococcus and Lactobacillus; the remaining isolates included Alcaligenes, Erwinia, Citrobacter, Klebsiella, Streptococcus, Bacillus and Arthrobacter.

Microbiological Evaluation of Selected Delicatessen Meats from Retail Supermarkets1

1980 ◽  
Vol 43 (7) ◽  
pp. 530-533 ◽  
Author(s):  
J. L. OBLINGER ◽  
J. E. KENNEDY
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Fecal Coliforms ◽  
Total Coliforms ◽  
E Coli ◽  
Fecal Streptococci ◽  
Improved Sanitation ◽  
Roast Beef ◽  
Yeasts And Molds

Roast beef, turkey breast, pastrami, corned beef and pickle and pimento loaf were obtained from delicatessens in eight Gainesville (FL) supermarkets. A total of 80 samples were analyzed for total aerobic counts (APC at 35 and 20 C), yeasts and molds, fecal streptococci, Staphylococcus aureus, Clostridium peifringens, total coliforms, fecal coliforms, Escherichia coli and Salmonella. No Salmonella was recovered from any samples and only one sample contained C. peifringens. S. aureus was recovered from 12.5% of the samples at levels less than 1 log/g and E. coli was found in 11.3% of the samples. All samples contained fecal streptococci and 73.8% contained coliforms. APCs (35 C) were highest in corned beef and pastrami samples with mean counts of 6.75 and 6.91 logs/g, respectively; roast beef samples had lowest APCs with a mean count of 4.84 logs/g. High APCs and coliform counts in many samples indicate a need for improved sanitation procedures at the processor and retail levels.

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from <3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

scholarly journals Comparison of Fecal Coliform Agar and Violet Red Bile Lactose Agar for Fecal Coliform Enumeration in Foods

2002 ◽  
Vol 68 (4) ◽  
pp. 1631-1638 ◽  
Author(s):  
A. Leclercq ◽  
C. Wanegue ◽  
P. Baylac
Keyword(s):  
Escherichia Coli ◽  
Fecal Coliform ◽  
Food Samples ◽  
Fecal Coliforms ◽  
Predictive Values ◽  
Direct Plating ◽  
Hafnia Alvei ◽  
E Coli ◽  
Plating Method ◽  
Mashed Potatoes

ABSTRACT A 24-h direct plating method for fecal coliform enumeration with a resuscitation step (preincubation for 2 h at 37 ± 1°C and transfer to 44 ± 1°C for 22 h) using fecal coliform agar (FCA) was compared with the 24-h standardized violet red bile lactose agar (VRBL) method. FCA and VRBL have equivalent specificities and sensitivities, except for lactose-positive non-fecal coliforms such as Hafnia alvei, which could form typical colonies on FCA and VRBL. Recovery of cold-stressed Escherichia coli in mashed potatoes on FCA was about 1 log unit lower than that with VRBL. When the FCA method was compared with standard VRBL for enumeration of fecal coliforms, based on counting carried out on 170 different food samples, results were not significantly different (P > 0.05). Based on 203 typical identified colonies selected as found on VRBL and FCA, the latter medium appears to allow the enumeration of more true fecal coliforms and has higher performance in certain ways (specificity, sensitivity, and negative and positive predictive values) than VRBL. Most colonies clearly identified on both media were E. coli and H. alvei, a non-fecal coliform. Therefore, the replacement of fecal coliform enumeration by E. coli enumeration to estimate food sanitary quality should be recommended.

scholarly journals Comparison of Fish Pond Waste Water with Manures under Garden Egg in Nigeria

2016 ◽  
Vol 6 (3) ◽  
pp. 58 ◽  
Author(s):  
Dominic J. Udoh ◽  
Otobong B. Iren ◽  
Jeremiah E. Jonathan
Keyword(s):  
Waste Water ◽  
Field Experiments ◽  
Crop Yields ◽  
Block Design ◽  
Poultry Manure ◽  
Fish Pond ◽  
Pig Manure ◽  
Soil Conditions ◽  
Total N ◽  
Cropping Seasons

<p class="1Body">Two field experiments were conducted from 2012 to 2013 cropping seasons to evaluate the efficacy and also determine the optimum rates of application for fish pond waste water in comparison with three other manures including one inorganic fertilizer and two organic manures under garden egg (<em>Solanum spp.</em>) crop.Garden egg was fertilized with pond waste water (PW), poultry manure (PM), pig manure (PG) and NPK15:15:15 at equivalent nitrogen (N) rates (0, 150, 300, 450 kg/ha). The treatments were arranged in a split plot under randomized complete block design (RCBD).The results obtained indicated a slight drop in soil pH but soil OM, total N, available P, exchangeable Ca and K increased generally with increasing rates of manures. The PW and PG treatments supported significantly (P&lt;0.05) the highest yields of the crop compared to PM and NPK with the 150 and 300 kg/ha treatments recording the highest increase. These results have shown that under the high rainfall and acidic soil conditions of Akwa Ibom State in Nigeria, PW is useful for the improvement of soil conditions and achievement of higher crop yields when applied at rates that supply N in the range of 150 – 300 kg/ha.</p>

scholarly journals Prevalence of fecal coliforms, Verotoxin Producing Escherichia coli, Campylobacter jejuni, C. coli, Salmonella and Listeria monocytogenes in Beef Offal

2015 ◽  
Vol 32 (4) ◽  
pp. 209-214
Author(s):  
Yukako SHIMOJIMA ◽  
Miki IDA ◽  
Yukari NISHINO ◽  
Rie ISHITSUKA ◽  
Sumiyo KURODA ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Campylobacter Jejuni ◽  
Fecal Coliforms

Occurrence and characterisation of ESBL-encoding plasmids among Escherichia coli isolates from fresh vegetables

2018 ◽  
Vol 219 ◽  
pp. 63-69 ◽  
Author(s):  
Christin Freitag ◽  
G.B. Michael ◽  
Jun Li ◽  
Kristina Kadlec ◽  
Yang Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fresh Vegetables

Microbiological Quality of Shellfish-Growing Waters in Chesapeake Bay

1994 ◽  
Vol 57 (3) ◽  
pp. 229-234 ◽  
Author(s):  
TUU-JYI CHAI ◽  
TZYY-JAN HAN ◽  
RALPH R. COCKEY
Keyword(s):  
Escherichia Coli ◽  
Chesapeake Bay ◽  
Water Samples ◽  
Microbiological Quality ◽  
Geographic Area ◽  
Water Area ◽  
Fecal Coliforms ◽  
Plate Count ◽  
Standard Plate Count ◽  
Standard Plate

A total of 338 water samples were collected at 20 stations from three geographically shellfish-growing areas in Chesapeake Bay from May to September 1989. Samples were examined for standard plate count, total coliforms, fecal coliforms, Escherichia coli and coliphages. Salinity, dissolved oxygen and temperature varied slightly with the depth, season, and geographic area of water samples. The geometric means of standard plate count for the three areas were 135, 355 and 275/ml, respectively. The range of means of fecal coliform for these areas was from &lt;3 to 93/100 mi. Escherichia coli counts were also low with a range of &lt;3 to 93/100 mi and a mean of &lt; 3/100 mi. The growing water area adjacent to cropland was found to have higher bacterial counts than those of the other two areas. Levels of male-specific phages were very low. Results indicate that shellfish-growing waters in all three areas were of satisfactory bacteriological quality.

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