A long-term study comparing membrane filtration with Colilert® defined substrates in detecting fecal coliforms and Escherichia coli in natural waters

2006 ◽  
Vol 80 (3) ◽  
pp. 191-197 ◽  
Author(s):  
D.W. Buckalew ◽  
L.J. Hartman ◽  
G.A. Grimsley ◽  
A.E. Martin ◽  
K.M. Register
Keyword(s):  
Escherichia Coli ◽  
Membrane Filtration ◽  
Natural Waters ◽  
Fecal Coliforms ◽  
Term Study ◽  
Long Term Study

scholarly journals Evaluation of homeopathic and biotherapic treatments in a swine farm to control Escherichia coli infection: a long term study

2021 ◽  
Vol 11 (40) ◽  
pp. 136-137
Author(s):  
Cidéli de Paula Coelho ◽  
Francisco Rafael Soto ◽  
Erlete Rosalina Vuaden ◽  
Priscilla Anne Melville ◽  
Andrea Micke Moreno ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Factors ◽  
Antimicrobial Treatment ◽  
Enterotoxigenic Escherichia Coli ◽  
Control Group ◽  
Swine Farm ◽  
Term Study ◽  
Mato Grosso ◽  
Long Term Study

Background: Escherichia coli is the most important etiological agent in neonatal diarrhea in swine, and Enterotoxigenic Escherichia coli (ETEC) is the most commonly isolated. Regarding to virulence factors, five main types of fimbriae were already described in swine samples: F4 (K88), F5 (K99), F6 (987P), F18 and F41. Thermolabile (LT) and thermostable enterotoxins (ST), as well as shiga-like toxin or verotoxin (Stx2) are also found in isolates of swine origin, related to diarrhoea process. Methods: This long term study has been developed in a swine farm (Mato Grosso, Brazil), in which 93 piglets were studied and 184 fecal samples were evaluated in two steps, with the aim to search the presence of Escherichia coli and to prepare a specific biotherapic medicine. Each step had one year of interval each other. Concurrently, for each step, a detailed anamnesis was made for choosing the ideal homeopathic medication for each step (Phosphorus 30 CH and Pulsatilla 30 CH, respectively). In each step, four groups consisting of 11-12 piglets and the respective primiparous mother pig were formed, and the treatments happened simultaneously: control group (antimicrobial treatment, the same used in the swine farm), homeopathic medication, E. coli biotherapic and homeopathic medication associated to biotherapic. The medications were made according to the Brazilian Homeopathic Pharmacopeia and the treatment lasted 12 days. After 24 days, in the weaning, the weight gain of each bath was also evaluated. Considering both steps, the research of virulence factors and enterotoxins was carried out in 99 Escherichia coli colonies through Polymerase Chain Reaction - PCR. Results: In both steps, the homeopathy treated groups passed from 75.0% of diarrhea incidence to 8.3% at the end of the treatment (p

scholarly journals Long-term study of the frequency of Escherichia coli and Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases

2005 ◽  
Vol 11 (8) ◽  
pp. 625-631 ◽  
Author(s):  
L. Romero ◽  
L. López ◽  
J. Rodríguez-Baño ◽  
J. Ramón Hernández ◽  
L. Martínez-Martínez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Term Study ◽  
Long Term Study ◽  
Extended Spectrum

scholarly journals A long-term study on the prevalence of shiga toxin-producing Escherichia coli (STEC) on four German cattle farms

2002 ◽  
Vol 129 (1) ◽  
pp. 173-185 ◽  
Author(s):  
L. GEUE ◽  
M. SEGURA-ALVAREZ ◽  
F. J. CONRATHS ◽  
T. KUCZIUS ◽  
J. BOCKEMÜHL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Pulsed Field Gel Electrophoresis ◽  
Single Group ◽  
Term Study ◽  
Virulence Markers ◽  
Long Term Study ◽  
Healthy Cattle ◽  
Cattle Farms

The occurrence of Shiga toxin-producing Escherichia coli (STEC) was studied on four cattle farms. STEC were detected in 29–82% of the cattle. STEC with additional EHEC markers were detected on all farms. The occurrence of the complete virulence marker pattern (stx1 and/or stx2, eae, EHEChlyA, katP, espP) was correlated with the presence of known STEC serotypes. STEC O26[ratio ]H11 and O165[ratio ]H25 with the complete pattern of virulence markers were the most prevalent. STEC O157 (H7/H-) STEC O103[ratio ]H2 and STEC O145[ratio ]H- were found sporadically. Five clonal subgroups of the STEC O26[ratio ]H11 isolates were identified by pulsed-field gel electrophoresis. STEC O26[ratio ]H11 were present in three groups of cattle. This serotype was detected in a single group over the entire fattening period. Most STEC O26[ratio ]H11 with the complete pattern of potential virulence markers were found in clinically healthy cattle. These animals may represent a risk factor for farmers and consumers.

Quinapril for Sclerosis Failed in Long-Term Study

2007 ◽  
Vol 38 (6) ◽  
pp. 14
Author(s):  
DAMIAN MCNAMARA
Keyword(s):  
Term Study ◽  
Long Term Study
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