scholarly journals Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Yan Zhang ◽  
Ping Wang ◽  
Youdong Xu ◽  
Xianli Meng ◽  
Yi Zhang
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Discriminant Analysis ◽  
Least Squares ◽  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Diabetic Rats ◽  
High Fat ◽  
Radix Puerariae

The main purpose of this study was to investigate the protective effects of total isoflavones from Radix Puerariae (PTIF) in diabetic rats. Diabetes was induced by a high-fat diet and intraperitoneal injection of low-dose streptozotocin (STZ; 40 mg/kg). At 26 weeks onwards, PTIF 421 mg/kg was administrated to the rats once daily consecutively for 10 weeks. Metabolic profiling changes were analyzed by Ultraperformance Liquid Chromatography-Quadrupole-Exactive Orbitrap-Mass Spectrometry (UPLC-Q-Exactive Orbitrap-MS). The principal component discriminant analysis (PCA-DA), partial least-squares discriminant analysis (PLS-DA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used for multivariate analysis. Moreover, free amino acids in serum were determined by high-performance liquid chromatography with fluorescence detector (HPLC-FLD). Additionally, oxidative stress and inflammatory cytokines were evaluated. Eleven potential metabolite biomarkers, which are mainly related to the coagulation, lipid metabolism, and amino acid metabolism, have been identified. PCA-DA scores plots indicated that biochemical changes in diabetic rats were gradually restored to normal after administration of PTIF. Furthermore, the levels of BCAAs, glutamate, arginine, and tyrosine were significantly increased in diabetic rats. Treatment with PTIF could regulate the disturbed amino acid metabolism. Consequently, PTIF has great therapeutic potential in the treatment of DM by improving metabolism disorders and inhibiting oxidative damage.

scholarly journals Ginsenoside Rb1 ameliorates Glycemic Disorder in Mice With High Fat Diet-Induced Obesity via Regulating Gut Microbiota and Amino Acid Metabolism

2021 ◽  
Vol 12 ◽  
Author(s):  
Xueyuan Yang ◽  
Bangjian Dong ◽  
Lijun An ◽  
Qi Zhang ◽  
Yao Chen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Sequencing Analysis ◽  
Ginsenoside Rb1 ◽  
High Fat ◽  
The Impact

Accumulating evidences suggested an association between gut microbiome dysbiosis and impaired glycemic control. Ginsenoside Rb1 (Rb1) is a biologically active substance of ginseng, which serves anti-diabetic effects. However, its working mechanism especially interaction with gut microbes remains elusive in detail. In this study, we investigated the impact of Rb1 oral supplementation on high fat diet (HFD) induced obesity mice, and explored its mechanism in regulating blood glucose. The results showed that higher liver weight and lower cecum weight were observed in HFD fed mice, which was maintained by Rb1 administration. In addition, Rb1 ameliorated HFD induced blood lipid abnormality and improved insulin sensitivity. Several mRNA expressions in the liver were measured by quantitative real-time PCR, of which UCP2, Nr1H4, and Fiaf were reversed by Rb1 treatment. 16S rRNA sequencing analysis indicated that Rb1 significantly altered gut microbiota composition and increased the abundance of mucin-degrading bacterium Akkermansia spp. compared to HFD mice. As suggested via functional prediction, amino acid metabolism was modulated by Rb1 supplementation. Subsequent serum amino acids investigation indicated that several diabetes associated amino acids, like branched-chain amino acids, tryptophan and alanine, were altered in company with Rb1 supplementation. Moreover, correlation analysis firstly implied that the circulation level of alanine was related to Akkermansia spp.. In summary, Rb1 supplementation improved HFD induced insulin resistance in mice, and was associated with profound changes in microbial composition and amino acid metabolism.

Plasma homocysteine level and hepatic sulfur amino acid metabolism in mice fed a high-fat diet

2012 ◽  
Vol 52 (1) ◽  
pp. 127-134 ◽  
Author(s):  
Kang Uk Yun ◽  
Chang Seon Ryu ◽  
Jung Min Oh ◽  
Chung Hyun Kim ◽  
Kye Sook Lee ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Homocysteine Level ◽  
Plasma Homocysteine ◽  
Sulfur Amino Acid ◽  
High Fat ◽  
Plasma Homocysteine Level

scholarly journals Sex-specific Metabolic Characterization of Serum and Liver of Mice with High-fat Diet

2020 ◽  
Author(s):  
Yi Zhou ◽  
Chen Li ◽  
Xinyi Wang ◽  
Qinbo Chen ◽  
Pengxi Deng ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Mice ◽  
High Fat ◽  
Metabolic Pattern ◽  
Male And Female ◽  
Female Mice

Abstract Background: Obesity exhibit sex differences is well established, but its metabolic mechanism remains unclear. Thus, investigation of metabolic pattern of male and female mice with high-fat diet (HFD) is of substantial importance for explore the potential mechanism linking gender differences in obesity.Methods: In the present study, we analyzed the metabolic changes in serum and liver of male and female mice with high-fat diet using nuclear magnetic resonance-based metabolomic approach.Results: Principle component analysis show that the metabolic pattern of serum and liver of male mice with HFD was significantly distinguished from the other groups. Furthermore, the accumulation of low-density lipoprotein/very low-density lipoprotein was found in the serum of male mice with HFD. Moreover, metabolomic results of liver reveal that tricarboxylic acid cycle and amino acid metabolism are increased in female mice with HFD.Conclusion: In conclusion, our results suggest that the differences in energy and amino acid metabolism of males and females were most likely influence the predisposition to obesity.

Analyses of fermentation products of Listeria species by frequency-pulsed electron-capture gas–liquid chromatography

1989 ◽  
Vol 35 (8) ◽  
pp. 786-793 ◽  
Author(s):  
Maryam I. Daneshvar ◽  
John B. Brooks ◽  
Georgia B. Malcolm ◽  
Leo Pine
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Electron Capture ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Gas Liquid Chromatography ◽  
Hydroxy Acids ◽  
Fermentation Products ◽  
Listeria Species ◽  
Fermentation Broths

Aerobic fermentation broths of eight Listeria monocytogenes strains, two or more strains of the remaining six Listeria species, and one strain of Jonesia denitrificans were examined by frequency-pulsed electron-capture gas–liquid chromatography for carboxylic acids, alcohols, amines, and hydroxy acids. All species produced acetic, isobutyric, butyric, isovaleric, phenylacetic, lactic, 2-hydroxybutyric, 2-hydroxyvaleric, and 2-hydroxyisocaproic acids. Propionic acid was not formed, and traces of isocaproic acid were observed. Of the alcohol and amine derivatives observed, only acetylmethylcarbinol, butylamine, and putrecine were identified. Recognition of the products of glucose and amino acid metabolism serves to further characterize the members of the genus Listeria both taxonomically and physiologically.Key words: Listeria, growth products, frequency-pulsed electron-capture gas–liquid chromatography.

scholarly journals A phosphoproteomic approach reveals that PKD3 controls PKA-mediated glucose and tyrosine metabolism

2021 ◽  
Vol 4 (8) ◽  
pp. e202000863
Author(s):  
Angel Loza-Valdes ◽  
Alexander E Mayer ◽  
Toufic Kassouf ◽  
Jonathan Trujillo-Viera ◽  
Werner Schmitz ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Biochemical Analysis ◽  
Acid Metabolism ◽  
Phenylalanine Hydroxylase ◽  
Molecular Targets ◽  
Protein Kinase D ◽  
High Fat ◽  
Tyrosine Metabolism

Members of the protein kinase D (PKD) family (PKD1, 2, and 3) integrate hormonal and nutritional inputs to regulate complex cellular metabolism. Despite the fact that a number of functions have been annotated to particular PKDs, their molecular targets are relatively poorly explored. PKD3 promotes insulin sensitivity and suppresses lipogenesis in the liver of animals fed a high-fat diet. However, its substrates are largely unknown. Here we applied proteomic approaches to determine PKD3 targets. We identified more than 300 putative targets of PKD3. Furthermore, biochemical analysis revealed that PKD3 regulates cAMP-dependent PKA activity, a master regulator of the hepatic response to glucagon and fasting. PKA regulates glucose, lipid, and amino acid metabolism in the liver, by targeting key enzymes in the respective processes. Among them the PKA targets phenylalanine hydroxylase (PAH) catalyzes the conversion of phenylalanine to tyrosine. Consistently, we showed that PKD3 is activated by glucagon and promotes glucose and tyrosine levels in hepatocytes. Therefore, our data indicate that PKD3 might play a role in the hepatic response to glucagon.

Arginine plus proline supplementation elicits metabolic adaptation that favors wound healing in diabetic rats

2012 ◽  
Vol 303 (10) ◽  
pp. R1053-R1061 ◽  
Author(s):  
A. Raynaud-Simon ◽  
L. Belabed ◽  
G. Le Naour ◽  
J. Marc ◽  
F. Capron ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Wound Healing ◽  
Amino Acid ◽  
Nitrogen Balance ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Diabetic Rats ◽  
Male Rats ◽  
Whole Body ◽  
Metabolic Adaptation

Diabetic patients with wounds are at risk of protein malnutrition, have low arginine plasma levels, and suffer from delayed wound healing. We sought to determine the efficacy of arginine plus proline supplementation on protein and amino acid metabolism and on wound repair in a model of diabetic rats. Eighteen 11-wk-old Zucker diabetic fatty fa/fa male rats underwent a 7-cm abdominal skin incision with implantation of sponges and daily excision of full-thickness round sections of dorsal skin for 5 days. They were randomized to be fed with either a standard formula (S group, Clinutren Iso), a high-protein and arginine (ARG) plus proline (PRO)-enriched formula (ARG+PRO group, Clinutren Repair), or an isonitrogenous isoenergetic control formula (IC group). Nitrogen balance was calculated daily. The rats were euthanized on day 5, and plasma glucose, insulin, amino acids, skin epithelialization, and angiogenesis were measured. In macrophages, we assessed inducible nitric oxide synthase (iNOS) and arginase expression, production of nitric oxide (NO) and amino acid metabolism. Both the ARG+PRO and IC groups showed improved nitrogen balance. ARG plus PRO supplementation increased proline and branched-chain amino acid plasma concentrations and improved angiogenesis. Arginase and iNOS expressions in macrophages were reduced, together with NO and citrulline production. In diabetic rats, ARG plus PRO supplementation improves wound angiogenesis and favors whole body protein metabolism. Low macrophage iNOS expression at day 5 may reflect a low inflammatory state in the wounds, favoring wound closure.

Sign in / Sign up

Export Citation Format

Share Document