Analyses of fermentation products of Listeria species by frequency-pulsed electron-capture gas–liquid chromatography

1989 ◽  
Vol 35 (8) ◽  
pp. 786-793 ◽  
Author(s):  
Maryam I. Daneshvar ◽  
John B. Brooks ◽  
Georgia B. Malcolm ◽  
Leo Pine
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Electron Capture ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Gas Liquid Chromatography ◽  
Hydroxy Acids ◽  
Fermentation Products ◽  
Listeria Species ◽  
Fermentation Broths

Aerobic fermentation broths of eight Listeria monocytogenes strains, two or more strains of the remaining six Listeria species, and one strain of Jonesia denitrificans were examined by frequency-pulsed electron-capture gas–liquid chromatography for carboxylic acids, alcohols, amines, and hydroxy acids. All species produced acetic, isobutyric, butyric, isovaleric, phenylacetic, lactic, 2-hydroxybutyric, 2-hydroxyvaleric, and 2-hydroxyisocaproic acids. Propionic acid was not formed, and traces of isocaproic acid were observed. Of the alcohol and amine derivatives observed, only acetylmethylcarbinol, butylamine, and putrecine were identified. Recognition of the products of glucose and amino acid metabolism serves to further characterize the members of the genus Listeria both taxonomically and physiologically.Key words: Listeria, growth products, frequency-pulsed electron-capture gas–liquid chromatography.

scholarly journals Metabolomic Analysis of Biochemical Changes in the Plasma of High-Fat Diet and Streptozotocin-Induced Diabetic Rats after Treatment with Isoflavones Extract of Radix Puerariae

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Yan Zhang ◽  
Ping Wang ◽  
Youdong Xu ◽  
Xianli Meng ◽  
Yi Zhang
Keyword(s):  
Liquid Chromatography ◽  
Amino Acid ◽  
Discriminant Analysis ◽  
Least Squares ◽  
High Fat Diet ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Diabetic Rats ◽  
High Fat ◽  
Radix Puerariae

The main purpose of this study was to investigate the protective effects of total isoflavones from Radix Puerariae (PTIF) in diabetic rats. Diabetes was induced by a high-fat diet and intraperitoneal injection of low-dose streptozotocin (STZ; 40 mg/kg). At 26 weeks onwards, PTIF 421 mg/kg was administrated to the rats once daily consecutively for 10 weeks. Metabolic profiling changes were analyzed by Ultraperformance Liquid Chromatography-Quadrupole-Exactive Orbitrap-Mass Spectrometry (UPLC-Q-Exactive Orbitrap-MS). The principal component discriminant analysis (PCA-DA), partial least-squares discriminant analysis (PLS-DA), and orthogonal partial least-squares discriminant analysis (OPLS-DA) were used for multivariate analysis. Moreover, free amino acids in serum were determined by high-performance liquid chromatography with fluorescence detector (HPLC-FLD). Additionally, oxidative stress and inflammatory cytokines were evaluated. Eleven potential metabolite biomarkers, which are mainly related to the coagulation, lipid metabolism, and amino acid metabolism, have been identified. PCA-DA scores plots indicated that biochemical changes in diabetic rats were gradually restored to normal after administration of PTIF. Furthermore, the levels of BCAAs, glutamate, arginine, and tyrosine were significantly increased in diabetic rats. Treatment with PTIF could regulate the disturbed amino acid metabolism. Consequently, PTIF has great therapeutic potential in the treatment of DM by improving metabolism disorders and inhibiting oxidative damage.

Amino Acid Metabolism

1979 ◽  
Vol 7 (1) ◽  
pp. 261-262
Author(s):  
E. V. ROWSELL
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism

Effect of intravenous bicarbonate administration on postoperative amino acid metabolism

1985 ◽  
Vol 4 ◽  
pp. 141-146 ◽  
Author(s):  
K VESTERBERG ◽  
J BERGSTROM ◽  
P FURST ◽  
U LEANDER ◽  
E VINNARS
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism

Different sensitivity of glucose and amino acid metabolism to insulin in NIDDM

Diabetes ◽  
1993 ◽  
Vol 42 (12) ◽  
pp. 1868-1877 ◽  
Author(s):  
L. Luzi ◽  
A. S. Petrides ◽  
R. A. De Fronzo
Keyword(s):  
Amino Acid ◽  
Amino Acid Metabolism ◽  
Acid Metabolism
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