scholarly journals Therapeutic and Safety Evaluation of Combined Aqueous Extracts ofAzadirachta indicaandKhaya senegalensisin Chickens Experimentally Infected withEimeriaOocysts

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
J. G. Gotep ◽  
J. T. Tanko ◽  
G. E. Forcados ◽  
I. A. Muraina ◽  
N. Ozele ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Broiler Chickens ◽  
Safety Evaluation ◽  
Serum Biochemistry ◽  
Stem Bark ◽  
Negative Control ◽  
Aqueous Extracts ◽  
Oxidative Stress Biomarkers ◽  
Treatment Groups ◽  
Dose Dependent Decrease

Coccidiosis is a disease of economic importance in poultry causing morbidity and mortality. Reports show thatAzadirachta indicaandKhaya senegalensishave been used individually in the treatment of avian coccidiosis. We thus investigated the efficacy and safety of the combined aqueous extracts of these plants for the treatment of experimentally induced coccidiosis in broiler chickens using oocyst count, oxidative stress biomarkers, serum biochemistry, histology, and haematological parameters. The phytochemical screening revealed the presence of tannins, saponins, cardiac glycosides, and steroids in both extracts. In addition, alkaloids and flavonoids were present inAzadirachta indica.There was significant (p<0.05) dose dependent decrease in oocyst count across the treatment groups with 400 mg/kg of the combined extract being the most efficacious dose. Immunomodulatory and erythropoietic activity was observed. There were decreased intestinal lesions and enhanced antioxidant activity across the treatment groups compared to the negative control. Administration of the combined extract did not cause damage to the liver as ALT, AST, and ALP levels were significantly reduced in the uninfected chickens treated with the extracts compared to control suggesting safety at the doses used. The combined aqueous extracts ofK. senegalensisstem bark andAzadirachta indicaleaves were ameliorative in chickens infected with coccidiosis.

scholarly journals Effect of Carica papaya L. Stem Bark Extracts on Cholesterol Concentration in Rats Induced with Streptosozin

2020 ◽  
Vol 151 ◽  
pp. 01011
Author(s):  
Safrida Safrida ◽  
Mustafa Sabri
Keyword(s):  
Body Weight ◽  
Carica Papaya ◽  
Stem Bark ◽  
Negative Control ◽  
Diabetic Rats ◽  
Cholesterol Concentration ◽  
Bark Extract ◽  
Treatment Groups ◽  
Randomized Design ◽  
Stem Bark Extract

This study was designed to determine the effect of Carica papaya L. stem bark extracts on cholesterol concentration in rats induced with glibenclamide. A completely randomized design was used for the experiment which consisted of 6 treatment groups, each group consisted of four rats, as follows:1) KN (negative control, non-diabetic rats); KP, diabetic rats given glibenclamide 10 mg/kg body weight; EP 1, diabetic rats given 0 mg/kg body weight/day extract; EP2, diabetic rats given 100 mg/kg body weight/day extract; and EP3, diabetic rats given 200 mg/kg body weight/day extract, EP4, diabetic rats given 300 mg/kg body weight/day extract for 28 day. The results showed that C. papaya L. stem bark extract decreased (P<0.05) cholesterol levels in diabetic rats. It was concluded that C. papaya L. stem bark extract had potential as anti-hypercholesterolemic in diabetic rats.

scholarly journals Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin

2011 ◽  
Vol 50 (No. 1) ◽  
pp. 31-39 ◽  
Author(s):  
H. Basmacıoglu ◽  
H. Oguz ◽  
M. Ergul ◽  
R. Col ◽  
Birdane YO
Keyword(s):  
Body Weight ◽  
Adverse Effects ◽  
Total Protein ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Serum Biochemistry ◽  
Inorganic Phosphorus ◽  
Broiler Chicks ◽  
Treatment Groups ◽  
Dietary Concentration

The amelioration of aflatoxicosis in broiler chickens was examined by feeding two concentrations of yeast component (esterified glucomannan; EG). EG, incorporated into the diet at 0.5 and 1 g/kg, was evaluated for its ability to reduce the detrimental effects of 2 mg total aflatoxin (AF; 82.72% AFB<sub>1</sub>, 5.50% AFB<sub>2</sub>, 10.20% AFG<sub>1</sub> and 1.58% AFG<sub>2</sub>) in diet on growing broiler chicks from 1 to 21 d of age. A total of 240 male broiler chicks (Ross-308) were divided into 6 treatment groups [control, AF, EG (0.5 g/kg), AF plus EG (0.5 g/kg), EG (1 g/kg), and AF plus EG (1 g/kg)]. Compared to the control, AF treatment significantly decreased body weight gain from week 2 onwards. AF treatment also caused significant decreases in serum total protein, albumin, total cholesterol, triglyceride, glucose, inorganic phosphorus, creatinine levels and alanine-aminotransferase (ALAT) activity but increased the aspartate-aminotransferase (ASAT) activity. Red blood cell, haematocrit, haemoglobin, thrombocyte, and lymphocyte counts and tibial crude ash levels were significantly reduced by AF treatment, while significant increases were seen in heterophil counts. The addition of EG (1 g/kg) to an AF-containing diet significantly improved the adverse effects of AF on haematological parameters, total protein, albumin values and ASAT activity. EG (1 g/kg) also partially improved body weight gains (59%) and the other biochemical parameters influenced by AF treatment. The addition of EG (both 0.5 and 1 g/kg) to the AF-free diet did not cause any considerable changes in the investigated values. These results clearly indicated that EG (1 g/kg) addition effectively diminished the adverse effects of AF on the investigated values. Also, the higher dietary concentration of EG (1 g/kg) was found more effective than the lower concentration (0.5 g/kg) against the adverse effects of AF on the variables investigated in this study. &nbsp;

scholarly journals Effect of multienzymes + probiotic supplementation in fermented shea butter cake based diets on the carcass traits and the biochemistry of broiler chickens

2020 ◽  
Vol 12 (3) ◽  
pp. 227-233
Author(s):  
P.C. Aguihe ◽  
A.S. Kehinde ◽  
S.K. Halidu ◽  
A. Sulyman ◽  
J. Chikezie ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Live Weight ◽  
Serum Biochemistry ◽  
Positive Control ◽  
Serum Glucose ◽  
Negative Control ◽  
Shea Butter ◽  
Broiler Chicks ◽  
Breast Meat ◽  
Randomized Design

Abstract. This study aimed to evaluate the effect of multienzyme+probiotic (MEP) supplementation on carcass characteristics and serum biochemistry of broiler chickens fed fermented shea butter cake meal (FSBCM) based diets. A total of two hundred and sixty (260), 28-day-old broiler chicks were distributed into four treatments, with five replicates of 13 birds each using a completely randomized design and reared on deep litter system from 28 to 56 days of age. The four treatments were: positive control – T1 (corn-soybean based diet); negative control – T2 (20% FSBCM replacement for corn in T1); diet 3 – T3 (T2 diet + 0.05% MEP) and diet 4 – T4 (T2 diet + 0.10% MEP). On day 56, two birds per each pen were selected for carcass and organ evaluation and other two birds per replicate were selected for serum biochemical analysis. The results indicated that addition of MEP to FSBCM diet caused a significant (p<0.05) increase in live weight, dressing percentage and breast meat yield than FSBCM diet without MEP and did not differ from the T1 diet. The abdominal fat in birds fed MEP treated diets were significantly (p<0.05) lower than those groups fed PC and FSBCM diets. Serum glucose was similar (p>0.05) between birds fed PC and MEP treated diets but significantly (p<0.05) higher than those on FSBCM diet, whereas serum cholesterol, alanine aminotransferase and aspartate aminotransferase concentrations reduced significantly (p<0.05) in groups fed MEP supplemented FSBCM diets. All other parameters were not significantly (p>0.05) affected by the MEP supplementation. Therefore, MEP supplementation in FSBCM based diet improved live weights and carcass attributes without any detrimental effect on blood composition of the broiler chickens.

scholarly journals Beta alanine effects immediately pre- and post hatch on chick quality, carcass yield and meat quality in broilers

2021 ◽  
Vol 51 (1) ◽  
Author(s):  
H. Atan ◽  
C. Kop-Bozbay
Keyword(s):  
Meat Quality ◽  
Broiler Chickens ◽  
Total Antioxidant Status ◽  
Negative Control ◽  
Access Time ◽  
In Ovo ◽  
Carcass Yield ◽  
Treatment Groups ◽  
Beta Alanine ◽  
Chick Quality

First, this study aimed to evaluate the effects on hatchability of in ovo supplementation of beta alanine (βA). Second, it assessed the effects on performance, meat quality and serum constituents of broiler chickens of βA injection in ovo and of supplementation with βA in feed for the first seven days after hatching. For this purpose, 960 fertilized broiler chicken eggs were distributed to hatchery trays in four treatment groups with six replicates on day 17 of incubation. The treatment groups consisted of eggs that were not injected (T1; negative control), injected with sterile solution with 0.9% salt (T2; positive control), injected with in ovo 1% βA solution (T3), and not injected, but with chicks were fed 1% βA for the first seven days after hatching (T4). Upon hatching, chicks from T1, T2, and T3 were fed commercial starter feed. All treatments were assessed with six replicates of 28 birds (14 females and 14 males) throughout the grow-out period. As a result of this study, T3 increased hatchability, yolk-free hatching weight and chick quality, and decreased yolk weight and feed access time at hatch. Both T3 and T4 improved carcass yield and meat quality, and T3 increased serum total antioxidant status at 42 days afer hatching. The results demonstrated that in ovo βA feeding improved hatching characteristics, and βA administration in ovo or in feed improved carcass yield and meat quality without affecting growth performance.

scholarly journals Screening for Toxic and Haematological Effect of Ethanol Extract of Azadirachta Indica in Wistar Rats Using Some Serum Enzymes as Markers

2020 ◽  
Vol 13 (1) ◽  
pp. 113-119
Author(s):  
K.K. Igwe ◽  
I.E. Otuokere ◽  
O. V. Ikpeazu
Keyword(s):  
Body Weight ◽  
Azadirachta Indica ◽  
Leukocyte Count ◽  
Negative Control ◽  
Serum Enzymes ◽  
Haematological Parameters ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Differential Leukocyte Count ◽  
Treatment Groups

The leaves of Azadirachta indica plant was screened for haematological, toxic and serum enzyme activities in rats. Twenty rats were used and were grouped into 4 of 5 rats each. Group 1 was the negative control group administered distilled water. Groups 2, 3, and 4 were the treatment groups received 200, 400 and 800 mg/kg body weight of the A. indica extract respectively. The rats were dosed for 14 days, thereafter were sacrificed and blood collected by cardiac puncture for analysis. The effect of A. indica extract was checked on haematological parameters and serum enzymes activities. All results in treatment groups were compared with the normal control at statistical confidence of 95% (p<0.05). There was progressive reduction of haematological parameters as the dose of the extract increased from 200, 400, to 800 mg/kg body weight. Haematological parameters, PCV, RBC, Haemoglobin showed decrease value which was not statistically significant at (p<0.05). Total leukocyte count, showed progressively elevation by the extract though not statistically significant. Differential leukocyte count indicated very mild lymphocytosis neutropeania, monocytopeania and eosinopeania which were not statistically significant. Clinical biochemical parameters, A. indica extract demonstrated normal levels of the serum enzymes (AST, ALT and ALP) though there was slight decrease in a dose dependent fashion.Total protein was within normal range. The normal MCV, MCH and MCHC values suggests normocytic normochromic anaemic condition. The extract of A. indica is safe to blood cells, liver and kidney marker enzymes at dose < 800 mg/kg body weight.

scholarly journals Nutritional value of tiger nut (Cyperus esculentus) offal on the performance of broiler chickens

2020 ◽  
Vol 47 (1) ◽  
pp. 174-185
Author(s):  
S. E. Alu ◽  
F. S. Oseze ◽  
E. J. Ogoshi
Keyword(s):  
Energy Source ◽  
Broiler Chickens ◽  
Nutritional Value ◽  
Growth Parameters ◽  
Control Diet ◽  
Serum Biochemistry ◽  
Blood Parameters ◽  
Metabolizable Energy ◽  
Treatment Groups ◽  
Significant Difference

Tiger nut offal is one of the common agro by products available in appreciable quantity all yaer round especially within the northern part of Nigeria. Its nutritional value is somewhat close to those of maize offal. Therefore, a 56 - day experiment was conducted to evaluate the response of broiler chickens fed graded levels of Tiger nut offal (TNO) as an energy source. Five iso-nitrogenous and iso-caloric diets tagged T1, T2, T3, T4 and T5, were compounded to contain 23 and 20% crude protein and 2800 and 3000 kcal/kg of metabolizable energy for starter and finisher phases, respectively. The TNO was included at 0, 5, 10, 15 and 20% levels in treatments, respectively such that T1 served as the control diet. A total of 200, one day old chicks were allotted to the 5 treatment groups, replicated 4 times and each replicate was allotted 10 birds. Parameters evaluated included growth rate, economics of production, blood parameters and carcass characteristics. The results showed that there was no significant variation (P>0.05) in the values obtained for growth parameters, economics of production, haematological parameters and serum biochemistry. However, there was significant difference (P<0.05) in dressed weight (1155.00 vs. 1350.00 vs. 1350.00 vs. 1525.00 and 1530.00 gbird-1 ) and shanks weight (59.50 vs. 100.00 vs. 67.00 vs. 85.00 and  60.00 gbird-1 ) which increased across the treatments as the levels of inclusion of TNO increased in the diets. Birds fed the diets 15 (T4) and 20% (T5) gave the best results compared to those fed the control and other levels. From the results of this experiment, poultry farmer can use up to 20% of TNO as a replacement for conventional energy source in the diet of broiler chickens without compromising the meat quality and health status of the birds.

scholarly journals COMPARATIVE ANALYSIS OF THE PHYTOCHEMICAL COMPOSITIONS OF LEAF, STEM-BARK AND ROOT OF AZADIRACHTA INDICA (NEEM)

2018 ◽  
Author(s):  
Nwali ON. ◽  
Idoko A. ◽  
Okolie JE ◽  
Ezeh E ◽  
Ugwudike PO ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Room Temperature ◽  
Reducing Sugars ◽  
Stem Bark ◽  
Aqueous Extracts ◽  
Leaf Sample ◽  
Plant Parts ◽  
Physico Chemical ◽  
Antifungal Action ◽  
Different Parts

Plant chemicals abound in different parts of plants, in different compositions. Thus, the comparative screening of the leaf, stem-bark and root of Azadirachta Indica becomes imperative. The presence of nine phytochemicals which include; Alkaloids, Flavonoids, Saponins, Glycosides, Phenols, Steroids, Tannins, Reducing sugars and Anthraquinones, and the quantity of alkaloids, flavonoids, saponins, tannins and glycoside in the leaf, stem-bark and root of Azadirachta indica were investigated. The plant parts were collected from a plantation grown in Okpotegu Echara, Ikwo Local Government Area of Ebonyi State, Nigeria. Plant samples were  air dried at room temperature, ground into uniform powder, sieved, bottled and labeled, ready for physico – chemical analysis. Results of the investigation revealed that anthraquinones were beyond detection limits in all the plant parts tested in both ethanol and aqueous extracts. Alkaloids were not detectable in leaf, stem-bark and root samples of aqueous extract. Glycosides were not detectable in leaf sample of ethanol and aqueous extracts. Quantitatively, the phytochemical compositions of each part showed higher concentrations of Alkaloids in the leaf, stem-bark and root (11.63%, 4.93% and 3.79%), compared to flavonoids (2.19%, 2.72% and 0.92%), saponins (0.70%, 1.12% and 0.44%), tannins (0.33 mg/100, 0.50mg/100 and 0.17mg/100) and glycosides (0.23%, 0.27% and 0.19%), respectively. Obviously, except for the higher percentage (11.63%) of alkaloids in the leaf, the phytochemicals in the stem-bark are higher as shown by the results, which could support the reason that the bark is preferably chewed commonly together with the stem as chew stick for its germicidal and antifungal action.

scholarly journals Efek Pemberian Ekstrak Etanol Daun Mimba (Azadirachta indica A. Juss) terhadap Struktur Histologis Pankreas Tikus Putih (Rattus norvegicus L.) Hiperglikemia

2021 ◽  
Vol 6 (2) ◽  
pp. 115-123
Author(s):  
Nunuk Shofiati ◽  
Siti Muflichatun Mardiati ◽  
Agung Janika Sitasiwi ◽  
Sri Isdadiyanto
Keyword(s):  
Azadirachta Indica ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Distilled Water ◽  
Treatment Groups ◽  
Randomized Design ◽  
Neem Leaves ◽  
Neem Leaf Extract

Indikator klinis penyakit Diabetes Melitus adalah hiperglikemia. Mimba (Azadirachta indica A. Juss) merupakan salah satu tumbuhan yang berpotensi sebagai alternatif obat herbal hiperglikemia. Penelitian ini bertujuan untuk menganalisis pengaruh ekstrak etanol daun mimba terhadap struktur histologis pankreas pada tikus hiperglikemia. Penelitian ini mengunakan Rancangan Acak Lengkap (RAL) dengan jumlah tikus 24 ekor yang dibagi menjadi 6 kelompok perlakuan dan 4 kali ulangan. P0 (kontrol normal) adalah kelompok tikus normal yang diberi akuades, P1 (kontrol negatif) adalah tikus hiperglikemia yang diberi akuades. P2 (kontrol positif) adalah kelompok tikus hiperglikemia yang diberi glibenklamid dosis 2,25 mg/kg BB. P3, P4, dan P5 adalah kelompok tikus yang diberi ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB. Data dianalisis dengan ANOVA pada signifikansi 95%. Hasil penelitian menunjukkan bahwa perlakuan ekstrak etanol daun mimba dosis 100, 200, dan 400 mg/kg BB tidak memberikan pengaruh nyata terhadap bobot pankreas, diameter, luas, dan densitas pulau Langerhans (P>0,05). Skoring struktur pulau Langerhans berdasarkan uji Mann-Whitneymenunjukkan hasil beda nyata pada kelompok  tikus yang diberi daun mimba (P≤0,05). Kesimpulan dari penelitian ini, pemberian ekstrak daun mimba dosis 400mg/kg BB menunjukkan adanya perbaikan morfologi pulau Langerhans. The clinical indicator of Diabetes mellitus was hyperglycemia. Azadirachta indica A. Juss was a plant has the potential to alternative medicine for hyperglycemia. The study was to analyze the ethanol neem leaf extract effect on histological structure of hyperglycemic rat pancreas. This study used a completely randomized design (CRD) with 24 rats were divided into 6 treatment groups and 4 replications. P0 (control) was a normal rats group were given distilled water, P1 (negative control) was a hyperglycemic rats group were given distilled water. P2 (positive control) was a hyperglycemic rats were given 2.25 mg/kg BW of glibenclamide. P3, P4, and P5 were rats were given 100, 200, and 400 mg/kg BW of ethanolic neem leaf extract.The data analyzed by ANOVA at 95% significance showed the treatment of 100, 200, and 400 mg/kg BW ethanolic neem leaf extract had no significant effect on the pancreatic weight, diameter, area, and density of Langerhans islet (P> 0.05). The score of Langerhans islet structure based on the Mann-Whitney test showed significant differences in the groups of mice given neem leaves (P≤0.05). Treatment of 400mg/Kg BW neem leaf extract showed an improvement in the morphology of the islets of Langerhans. 

scholarly journals Oxidative Stress Induced DNA Damage and Reproductive Toxicity in Male Albino Mice Orally Exposed to Sorbitol

2019 ◽  
Vol 4 (2) ◽  
pp. 46-58 ◽  
Author(s):  
Okunola A. Alabi ◽  
Lanre R. Oladimeji ◽  
Adewale A. Sorungbe ◽  
Yetunde M. Adeoluwa
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Sperm Count ◽  
Reproductive Toxicity ◽  
Negative Control ◽  
Daily Consumption ◽  
Oxidative Stress Biomarkers ◽  
Treatment Groups ◽  
Polychromatic Erythrocytes ◽  
Dose Dependent

AbstractIn this study, the potential DNA damage and reproductive toxicity of sorbitol was investigated using bone marrow micronucleus (MN), sperm morphology, and sperm count in mice. Five doses of 90, 45, 20, 10 and 1 mg/kg/day, defined by allometry, and approximately corresponding to 1.5g, 750mg, 330mg, 165mg and 16mg of sorbitol daily consumption by a 70kg human, respectively, were used. MN analysis showed a dose-dependent induction of micronucleated polychromatic erythrocytes and other nuclear abnormalities across the treatment groups. Assessment of sperm shape showed a significant (p < 0.05) increase in sperm abnormalities with significant (p < 0.05) decrease in mean sperm count in treated groups. The result of the oxidative stress biomarkers showed induction of significant (p < 0.05) increase in liver catalase, MDA and serum ALT and AST activities with concomitant decrease in SOD activities in exposed mice. A significant increase in weight of exposed mice were recorded when compared with the negative control. The results of this study showed the genotoxicity and reproductive effects of sorbitol.

scholarly journals Effect of Bacillus subtilis on the microarchitectural development of the immune system in Salmonella-challenged broiler chickens

2021 ◽  
Author(s):  
A Sikandar ◽  
H Zaneb ◽  
A Nasir ◽  
A Rehman ◽  
M Kashif ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Broiler Chickens ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Salmonella Gallinarum ◽  
Treatment Groups ◽  
Immune Organs ◽  
Prophylactic Use ◽  
And Function

The effect of Bacillus subtilis on the immune responses and morphometry of the immune organs was evaluated in broilers challenged with S. gallinarum.  For this purpose, Salmonella-free birds (n = 240) were split into four groups with six replicates of ten birds each. Groups included an NC (negative control, non-infected + non-medicated), a PC-S (positive control, Salmonella-infected + non-medicated), an AT-S (Salmonella-infected + medicated with enrofloxacin), and a BS-S (Salmonella-infected + B. subtilis (2.0 × 10<sup>10</sup> cfu/g; 0.1 g/kg) group. On day 21, the thickness of the thymus cortex and medulla, germinal centre area of the spleen, bursal follicular length and bursal follicular area increased (P &lt; 0.05) in the BS-S when compared to the NC and PC-S groups. On day 35, the BS-S group exhibited a higher (P &lt; 0.05) antibody titre against the Newcastle disease virus (NDV), and cortex of the thymus was thicker (P &lt; 0.05) compared to the other groups. A decrease in the thymus medulla thickness, germinal area of the spleen and bursal follicular number were noted in the PC-S group when compared to the other treatment groups. In conclusion, the prophylactic use of B. subtilis type probiotics alleviated the stress resulting from a Salmonella gallinarum infection and improved the immune organs development and function in infected broilers.

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