scholarly journals GSTP1Methylation and Protein Expression in Prostate Cancer: Diagnostic Implications

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Filippo Martignano ◽  
Giorgia Gurioli ◽  
Samanta Salvi ◽  
Daniele Calistri ◽  
Matteo Costantini ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Basal Cell ◽  
Cell Layer ◽  
Methylation Status ◽  
Intraepithelial Neoplasia ◽  
Basal Cell Layer ◽  
Gene Methylation ◽  
Specific Pcr ◽  
Cycle Regulation ◽  
Early Carcinogenesis

GSTP1belongs to the GSTs family, a group of enzymes involved in detoxification of exogenous substances and it also plays an important role in cell cycle regulation. Its dysregulation correlates with a large variety of tumors, in particular with prostate cancer. We investigatedGSTP1methylation status with methylation specific PCR (MS-PCR) in prostate cancer (PCa) and in benign tissue of 56 prostatectomies. We also performed immunohistochemistry (IHC) so as to correlate gene methylation with gene silencing.GSTP1appears methylated in PCa and not in healthy tissue; IHC confirmed that methylation leads to protein underexpression (p<0.001). GSTP1 is highly expressed in basal cell layer and luminal cells in benign glands while in prostatic intraepithelial neoplasia (PIN) it stains only basal cell layer, whereas PCa glands are completely negative. We demonstrated that methylation leads to underexpression of GSTP1. The progressive loss of GSTP1 expression from healthy glands to PIN and to PCa glands underlines its involvement in early carcinogenesis.

scholarly journals Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions

2019 ◽  
Vol 13 (2) ◽  
pp. 56-58
Author(s):  
Inas A. Rasheed
Keyword(s):  
Basal Cell ◽  
Prostatic Carcinoma ◽  
Cell Layer ◽  
Intraepithelial Neoplasia ◽  
Prostatic Intraepithelial Neoplasia ◽  
Cell Detection ◽  
Basal Cells ◽  
Basal Cell Layer ◽  
Cell Markers ◽  
Malignant Lesions

Background: The diagnosis of prostatic pathology may be of challenging , as some  difficult and suspected, atypical  cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from  malignant prostatic lesions. Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions. Type of the study:  A retro-spective  study. Methods:  115cases of  paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hyperplasia( BPH) , 9 cases were  high grade –prostatic intraepithelial neoplasia (HG-PIN) , and 30 cases were prostatic carcinoma(PCa) .Sections from each blocks were prepared for immunostaining with 34BE12 and p63. Results : basal cells were detected in cases of BPH , and HG-PIN , and absent  in all cases of prostatic carcinoma ,using basal cell markers . Negative benign glands(>2) were found in 71.6% and 38.2%  for   BPH  and 57.1% and 55.6% for HG-PIN immunostained  with high molecular weight cytokeratin (34BE12) and p63 , respectively, and significantly reduced to 9.0% and 11.1% for BPH and HG-PIN, respectively  with combined using of both markers .Conclusion : Combination of both   basal cell markers (34BE12 , p63) improving basal cell detection in atypical ,suspected prostatic lesions and distinguish benign from malignant lesions.

Stratification of Atypical Intraepithelial Prostatic Lesions Based on Basal Cell and Architectural Patterns

2019 ◽  
Vol 153 (3) ◽  
pp. 407-416 ◽  
Author(s):  
Guang-Qian Xiao ◽  
Reza Golestani ◽  
Huy Pham ◽  
Andy E Sherrod
Keyword(s):  
Basal Cell ◽  
Cell Layer ◽  
Intraepithelial Neoplasia ◽  
Low Grade ◽  
High Grade ◽  
Basal Cells ◽  
Basal Cell Layer ◽  
Clinical Patient ◽  
Architectural Patterns ◽  
Intraepithelial Carcinoma

Abstract Objectives: High-grade prostatic intraepithelial neoplasia (HPIN) and atypical cribriform lesion of the prostate are considered the precursors or associators of invasive prostate cancer (iPCa). Given loss of basal cells being the hallmark of iPCa, we hypothesized that a subset of these atypical intraepithelial lesions (AILs) with sparse basal cells can be classified as prostatic intraepithelial carcinoma (PIC) with frequent iPCa association and that different morphologic patterns of PIC are associated with specific Gleason (G) patterns and scores for iPCa. Methods: We stratified 153 foci of AILs from 110 patients based on the integrity of the basal cell layer and architectural patterns and their association with iPCa. Results: We demonstrated that AILs could be stratified into usual HPIN (intact basal cell layer and simple patterns) with low-risk of iPCa association and PIC (sparse basal cell layer) with high risk of iPCa association. Furthermore, PIC could be divided into low-grade (simple patterns and associated with G3 and G3/4 iPCa) and high-grade PIC (complex patterns and associated with G4 and G3/4/5 iPCa). Conclusions: Such stratification is of great clinical significance and instrumental to clinical patient management. It not only increases the predictability of AILs for iPCa but also accommodates a clinical scenario for lesions with features of intraductal carcinoma when iPCa is not found, particularly in biopsies.

Histopathological varieties of oral carcinomain situ: Diagnosis aided by immunohistochemistry dealing with the second basal cell layer as the proliferating center of oral mucosal epithelia

2010 ◽  
Vol 60 (3) ◽  
pp. 156-166 ◽  
Author(s):  
Takanori Kobayashi ◽  
Satoshi Maruyama ◽  
Jun Cheng ◽  
Hiroko Ida-Yonemochi ◽  
Minoru Yagi ◽  
...  
Keyword(s):  
Basal Cell ◽  
Cell Layer ◽  
Basal Cell Layer ◽  
Carcinomain Situ

scholarly journals Morphological markers of secretory activity in prostatic adenocarcinoma

2007 ◽  
Vol 64 (9) ◽  
pp. 617-622 ◽  
Author(s):  
Ljubinka Jankovic-Velickovic ◽  
Vuka Katic ◽  
Ivan Ignjatovic
Keyword(s):  
Prostate Cancer ◽  
Mixed Type ◽  
Cell Layer ◽  
Secretory Activity ◽  
Gleason Grade ◽  
Morphological Markers ◽  
Adult Males ◽  
Basal Cell Layer ◽  
Mucin Secretion ◽  
Infiltrative Growth Pattern

Background/Aim. The vast majority of prostatic tumors developing in adult males are adenocarcinomas (ACP). Histological diagnosis of prostate cancer relies on the infiltrative growth pattern, presence of macronucleoli, and absence of basal cell layer. In the last few years a special attention has been focused on investigation of cancerous glands lumen content. The aim of the study was to determine the presence of intraluminal (crystaloids, blue-tanged mucinous secretions and distension of glands) and extracellular markers of pathological secretions in ACP, as well as the type of intraluminal and extracellular mucinous secretions. Methods. Micromorphological and hystochemical (PAS, HIDAB pH 2.5) analysis was done in 96 patients to detemine differentiation by Gleason and markers of secretory activity. Results. In ACP neoplastic gland`s distension (53.12%), crystalloids (30.2%), basophilic secretions (26.04%) and presence of extracellular mucins (8.3%) were found. All morphological signs were detected in carcinoma of Gleason grade 1-4A. There was significant presence of crystalloids ((G1+G2)/G4 p < 0.05; G3/G4A p < 0.05), basophilic secretions (G3/G2 p < 0.05; G3/G4A p < 0.05) and extracellular mucins (G3/G4A p < 0.001). The association of these morphological features with neutral and acidic mucins secretion was statisticaly significant (p < 0.0001). Conclusion. This study showed that intraluminal and extracellular markers of secretory activity in ACP were significantly more often associated by mixed type of mucin secretion with predominace of sialomucins. .

scholarly journals Basal Cell Adenoma of the Upper Lip from Minor Salivary Gland Origin

2008 ◽  
Vol 02 (03) ◽  
pp. 213-216 ◽  
Author(s):  
Eliana Maria Minicucci ◽  
Eloisa Bueno Pires de Eloisa ◽  
Silke Anna Thereza Weber ◽  
Maria Aparecida Custodio Domingues ◽  
Daniel Araki Ribeiro
Keyword(s):  
Salivary Gland ◽  
Basal Cell ◽  
Cell Layer ◽  
Minor Salivary Gland ◽  
Salivary Gland Neoplasm ◽  
Basal Cell Adenoma ◽  
Parotid Glands ◽  
Upper Lip ◽  
Basal Cell Layer ◽  
Cell Adenoma

ABSTRACTBasal cell adenoma is an uncommon benign salivary gland neoplasm, presenting isomorphic basaloid cells witha prominent basal cell layer. Taking into account that basal cell adenomas represent 1% of all salivary gland tumors, being the majority of cases in the parotid glands, the goal of this paper is to report a case of basalcell adenoma of the upper lip arising from minor salivary gland. (Eur J Dent 2008;2:213-216)

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