scholarly journals Evaluation of Hypoglycemic and Genotoxic Effect of Polyphenolic Bark Extract from Quercus sideroxyla

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Marcela Soto-García ◽  
Martha Rosales-Castro ◽  
Gerardo N. Escalona-Cardoso ◽  
Norma Paniagua-Castro
Keyword(s):  
Acute Toxicity ◽  
Phenolic Compound ◽  
Diabetic Control ◽  
Genotoxic Effect ◽  
Hypoglycemic Activity ◽  
Bark Extract ◽  
Total Phenolic ◽  
Glucose Tolerance Curve ◽  
Tolerance Curve

Quercus sideroxyla is a wood species whose bark has phenolic compound and should be considered to be bioactive; the hypoglycemic and genotoxic properties of Q. sideroxyla bark were evaluated in this study. Total phenolic compound was determined in crude extract (CE) and organic extract (OE). The OE has the highest amount of phenols (724.1±12.0 GAE/g). Besides, both CE and OE demonstrated effect over the inhibition of α-amylase in vitro. Hypoglycemic activity was assessed by glucose tolerance curve and the area under curve (UAC); OE showed the highest hypoglycemic activity. In addition, diabetes was induced by streptozotocin (65 mg/kg) and the extracts (50 mg/kg) were administered for 10 days; OE showed hypoglycemic effect compared with diabetic control and decreased hepatic lipid peroxidation. Acute toxicity and genotoxicity were evaluated in CE; results of acute toxicity did not show any mortality. Besides, the comet assay showed that CE at a dose of 100 mg/kg did not show any genotoxic effect when evaluated at 24 h, whereas it induced slight damage at 200 mg/kg, with the formation of type 1 comets.

scholarly journals TLC profiling and antioxidant activity of phenolic compound from Sterculia oblongata bark extract

2019 ◽  
Vol 11 (1) ◽  
pp. 44-48 ◽  
Author(s):  
TRI MURNINGSIH ◽  
PRAPTIWI PRAPTIWI ◽  
LIANA LIANA ◽  
AHMAD FATHONI
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Bark Extract ◽  
Total Phenolic ◽  
Β Carotene ◽  
In Vitro Antioxidant Activity

Abstract. Murningsih T, Praptiwi, Liana, Fathoni A. 2019. TLC profiling and antioxidant activity of phenolic compound from Sterculia oblongata bark extract. Nusantara Bioscience 11: 44-48. In the present study, we investigated the total phenolic content and in- vitro antioxidant activity of Sterculia oblongata. Total phenolic content (TPC) of the methanol extract was examined by Folin-Ciocalteu reagent and gallic acid as standard. The in-vitro antioxidant activity was performed by DPPH free radical scavenging activity and β-carotene bleaching assay. The result showed that the TPC of methanol extract was the highest (P<0.05) compared to other extracts. There is a positive and significantly very high Pearson’s correlation between antioxidant activity index (AAI) and TPC (r = 0.996). A negative and significantly high Pearson’s correlation was obtained between IC50 value and TPC (r = -0.885). The result of β-carotene bleaching assay showed that the rate of the decreased antioxidant activity of S.oblongata extract was lower than that of β-carotene/linoleic acid. The results suggest that the extract of S. oblongata can be used as a natural antioxidant. However, further investigation to isolate and identify the chemical compounds responsible for antioxidant was needed

scholarly journals Изучение противовоспалительной активности экстракта змееголовника молдавского

2020 ◽  
Author(s):  
E.N. Kurmanova ◽  
E.V. Ferubko ◽  
L.B. Strelkova ◽  
R.K. Kurmanov ◽  
O.P. Sheichenko
Keyword(s):  
Acute Toxicity ◽  
Toxic Substance ◽  
Inflammatory Activity ◽  
Total Phenolic ◽  
Dry Extract ◽  
Low Toxic ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Змееголовник молдавский (Dracocephalum moldavica L.) в народной медицине используется в качестве противовоспалительного, ранозаживляющего, отхаркивающего и седативного средства. В ФГБНУ ВИЛАР разработан змееголовника молдавского травы экстракт сухой под условным названием «Люкатил» (сумма фенольных соединений 64,12% в пересчёте на цинарозид). Цель работы - изучение острой токсичности и противовоспалительной активности экстракта змееголовника для разработки на его основе лекарственного препарата. Методика. Проведено определение параметров острой токсичности и противовоспалительной активности экстракта. При изучении острой токсичности экстракта по методу Кербера использованы белые нелинейные мыши-самцы в количестве 30 особей. «Люкатил» вводили животным внутрижелудочно в дозах 500, 1000, 1500 и 2000 мг/кг. Для выявления противовоспалительной активности экстракта змееголовника молдавского использована in vitro ферментная биотест-система на основе индуцибельной NO-синтазы. Для выявления противовоспалительной активности экстракта in vivo использованы нелинейные мыши-самцы. Оценку влияния экстракта в дозе 200 мг/кг на экссудативную стадию воспаления проводили на модели 1% формалинового отёка. В качества препарата сравнения использовали индометацин (5 мг/кг). Формалиновый отёк вызывали однократным субплантарным введением под апоневроз задней правой лапки мыши 0,05 мл 1% формалина в качестве флогогенного агента. Величину отёка определяли по разнице в массе лапок контрольных и опытных животных и рассчитывали процент снижения степени отёка. Результаты. При однократном введении экстракт «Люкатил» не приводил к гибели животных, изменения внешнего вида и поведенческих реакций мышей не наблюдалось. В соответствии с классификацией токсичности химических веществ по ГОСТ 12.1.007-76 «Люкатил» является малотоксичным веществом. In vitro установлена высокая противовоспалительная активность экстракта, при этом остаточная активность iNOS снижалась до 25%. Экстракт в дозе 200 мг/кг in vivo обладал статистически значимым противовоспалительным эффектом. Он подавлял развитие экссудативной фазы воспаления на 33,7%, по сравнению с контрольной группой животных, уступая противовоспалительному эффекту индометацина. Заключение. Змееголовника молдавского травы экстракт сухой под условным названием «Люкатил» является малотоксичным веществом, обладает выраженным противовоспалительным эффектом в опытах in vitro, in vivo и является перспективным объектом для дальнейшего фармакологического изучения в качестве противовоспалительного лекарственного средства.Moldavian dragonhead (Dracocephalum moldavica L.) is used in traditional medicine as an anti-inflammatory, wound-healing, expectorant, and sedative means. In our Institute, a Moldavian dragonhead herb dry extract (total phenolic content, 64.12% in cynaroside equivalent) was developed and conventionally named Lyukatil. Objective. To study acute toxicity and anti-inflammatory activity of the dragonhead extract for developing a drug based on this extract. Method. Parameters of acute toxicity and anti-inflammatory activity of the extract were assessed. The study of acute toxicity of the extract was performed using the Kerber method on male white mongrel mice (n=30). Lyukatil was administered to the animals intragastrically at doses of 500 mg/kg, 1000 mg/kg, 1500 mg/kg, and 2000 mg/kg. Anti-inflammatory activity of the Moldavian dragonhead extract was determined in vitro using an enzyme Biotest system based on inducible NO synthase. Mongrel male mice were used to study the anti-inflammatory activity of the extract in vivo. The effect of the extract at a dose of 200 mg/kg on the exudative phase of inflammation was evaluated on a model of 1% formalin-induced edema. Indomethacin 5 mg/kg was used as a reference drug. Formalin edema was induced by a single subplantar injection of 0.05 ml of 1% formalin as a phlogogenic agent under the aponeurosis of the right hind leg. The degree of edema was determined by the difference in leg weights in control and experimental animals; then the decrease in edema was calculated in per cent. Results. A single administration of the extract Lyukatil did not cause death of animals, changes in the appearance or in behavioral responses, shortness of breath, or drowsiness. In accordance with the toxicity classification for chemical substances as per GOST Standard 12.1.007-76, Lyukatil is a low-toxic substance. The extract at a dose of 200 mg/kg exerted a significant anti-inflammatory effect as shown by suppression of the exudative phase of formalin-induced inflammation by 33.7% compared to the control group. However, this effect was inferior to the anti-inflammatory effect of indomethacin. Conclusions. The Moldavian dragonhead herb dry extract under the conventional name of Lyukatil is a low-toxic substance that has a significant anti-inflammatory effect both in vitro and in vivo and is a promising target for further pharmacological studies as an anti-inflammatory drug.

scholarly journals Phytopharmacological Evaluation of Alcoholic Extract of Berberis aristata Leaf in the Treatment of Gastric Ulcer

2020 ◽  
Vol 13 (1) ◽  
pp. 1-5
Author(s):  
Ratnaker Singh ◽  
Y. Trilochana
Keyword(s):  
Acute Toxicity ◽  
Crude Extract ◽  
Antioxidant Activities ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Antiulcer Activity ◽  
Alcoholic Extract ◽  
Dose Dependent

For over a century, peptic ulcer has been one of the most common gastrointestinal tract (GIT) disorder. There are number of drugs are now available for treatment. Drugs of herbal origin reduce the offensive factors and have proved to be safe, clinically effective, relatively less expensive, globally competitive, and with better patient tolerance.This study was performed to assess the anti-ulcer activity on different parts of B.aristata. Apart from that, acute toxicity, qualitative chemical analysis, total phenolic content (TPC), total flavonoid content(TFC) and in vitro antioxidant activities were evaluated. The potentially active plant part was selected for screening as gastro protective, in vivo antioxidant and antisecretory activities in ulcerated rats.The 50% ethanolic extract of B. aristata were subjected to preliminary phytochemical screening, estimation of TFC and TPC. The crude extract from the leaves of B. aristata gave best antiulcer activity among flower and stem. In acute toxicity studies, the administration of the crude extract of B. aristata leaves did not reveal any adverse effects or toxicity in rats at fourteen days observations.The results of these studies have shown that ethylexract of B.aristata leaf (EEBAL) produced a significant dose dependent ulcerprotective, antioxidant and antisecretory activity by blocking the activity of proton pump, protecting from antioxidants produced during stress induced ulcer and by enhancing glycoprotein levels.

scholarly journals CHEMICAL CONSTITUENTS, IN VITRO ANTIOXIDANT ACTIVITY, ORAL ACUTE TOXICITY AND LD50 DETERMINATION OF MORINGA OLEIFERA LEAVES

2017 ◽  
Vol 9 (5) ◽  
pp. 240
Author(s):  
Manal Mortady Hamed ◽  
Aboelfetoh Mohamed Abdalla ◽  
Mosad Ahmed Ghareeb ◽  
Said Abdelhalim Saleh
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Dry Extract ◽  
Phenolic Contents ◽  
Moringa Oleifera Leaves ◽  
Oral Acute Toxicity

Objective: The objective of this study was undertaken to estimate the total phenolic contents (TPCs), in vitro antioxidant of different solvent extracts of M. oleifera leaves, oral acute toxicity and LD50 determination of the 85% methanolic extract as well as the chromatographic isolation and identification of the extract constituents.Methods: The antioxidant activity of different solvent extracts of Moringa oleifera leaves were estimated using three antioxidant assays and the total phenolic contents (TPCs) were also evaluated using Folin-Ciocalteu’s assay. The n-BuOH extract undergoes further chromatographic isolation owing to the high antioxidant activity using 2, 2'-diphenyl-1-picrylhydrazyl radical (DPPH) method, which resulted in the isolation of seven compounds.Results: The results showed that the TPCs values of the tested extracts were varied from 309.52 to 43.28 mg gallic acid equivalent/g dry extract. The reducing power antioxidant activities (RPAA) were 0.434, 0.402, 0.395, 0.149, 0.143 and 0.124, while the total antioxidant capacity (TAC) values were 316.43, 203.35, 181.56, 86.70, 76.62 and 50.83 mg ascorbic acid equivalent/g dry extract; for n-BuOH, EtOAc, 85% MeOH, H2O, CH2Cl2, and pet. ether extracts, respectively. The oral acute toxicity study of the 85% methanol extracts of M. oleifera and M. peregrina revealed that; their LD50 values were 3458.3 and 4125 mg/kg respectively, thus the two plants could be classified as slightly toxic in the scale of Hodge and Sterner which reflected their nutrient values as edible plants. The isolated compounds were identified on the basis of their 1H and 13C-NMR spectra as; cis-p-coumaric acid 4-O-(2'-O-β-D-apiofuranosyl)-β-D-glucopyranoside (1), chlorogenic acid (2), niazirin (3), 3,4-dihydroxy-β-phenylethoxy-O-α-L-rhamnopyranosyl-(l→2)-α-L-rhamnopyranosyl-(1→3)-4-O-caffeoyl-β-D-glucopyranoside (4), gallic acid (5), taxifolin (6), and benzyl-carbamo-thioethionate (7).Conclusion: The M. oleifera leaves showed promising antioxidant activities and slightly toxic behavior.

Antioxidant and Analgesic Activity of Acer laevigatum Wall, a Traditional Mizo-tribe Medicine

2020 ◽  
Vol 13 (6) ◽  
pp. 5200-5207
Author(s):  
Andrew Lalthasanga Ralte ◽  
Phaibiang Lapasam ◽  
Freddy Teilang Nongkhlaw ◽  
Pdiangmon Kyndait ◽  
Zothanpuia
Keyword(s):  
Antioxidant Activity ◽  
Acute Toxicity ◽  
Analgesic Activity ◽  
Methanolic Extract ◽  
Bark Extract ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
External Application ◽  
Soxhlet Apparatus

Acer laevigatum is an evergreen tree growing to a height of 10–15 m or more, with a trunk up to 50 cm diameter belonging to the family Sapindaceae. In Mizoram, the decoction of the leaves is used as an external application in sprains. Extraction was carried out by drying the leaves and barks and extracted by using methanol as solvent using the Soxhlet apparatus. Preliminary phytochemical screening was carried out by methanolic extract of both leaves and barks to determine the chemical constituents present in the plant using a different phytochemical test, acute toxicity for leave and bark extract, in-vitro antioxidant activity and in-vivo analgesic activity of barks extract. Phytochemical screening was performed for both extract and it contains glycoside, saponin, phenol, tannin, flavonoid, and steroid. The antioxidant activity test of the methanolic extract of bark extract was performed successfully. In acute toxicity, the LD50 was found that for more than 2000 mg/kg body weight was safe for further uses. The total phenolic content of the bark extract contains 493 ± 0.23 mg of GAE/g and the total flavonoids content of the bark extract was 220 ± 0.034 mg of QE/g. The IC50 value of DPPH free radical scavenging activity was found to be 86.1211 µg/ml and nitric oxide was 75.9 µg/ml. Whereas, in reducing power it was found that the percentage inhibition was increased with an increase in concentration (increase in concen-tration, percentage inhibition was also increased) and reduced Fe3+ (ferricyanide complex) to Fe2+ (ferrous form). Finally, for in-vivo analgesic activity, 4000 mg/kg was more effective than 2000 mg/kg of the bark extract. These results confirm that the methanolic extract of bark of Acer laevigatum possesses antioxidant activity and non-significant or less analgesic activity.

scholarly journals In Vivo Antiplasmodial Activity of Terminalia mantaly Stem Bark Aqueous Extract in Mice Infected by Plasmodium berghei

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Mariscal Brice Tchatat Tali ◽  
Cedric Derick Jiatsa Mbouna ◽  
Lauve Rachel Yamthe Tchokouaha ◽  
Patrick Valere Tsouh Fokou ◽  
Jaures Marius Tsakem Nangap ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Aqueous Extract ◽  
Plasmodium Berghei ◽  
Antimalarial Activity ◽  
Lethal Dose ◽  
Stem Bark ◽  
Antiplasmodial Activity ◽  
Bark Extract

Background. Terminalia mantaly is used in Cameroon traditional medicine to treat malaria and related symptoms. However, its antiplasmodial efficacy is still to be established. Objectives. The present study is aimed at evaluating the in vitro and in vivo antiplasmodial activity and the oral acute toxicity of the Terminalia mantaly extracts. Materials and Methods. Extracts were prepared from leaves and stem bark of T. mantaly, by maceration in distilled water, methanol, ethanol, dichloromethane (DCM), and hexane. All extracts were initially screened in vitro against the chloroquine-resistant strain W2 of P. falciparum to confirm its in vitro activity, and the most potent one was assessed in malaria mouse model at three concentrations (100, 200, and 400 mg/kg/bw). Biochemical, hematological, and histological parameters were also determined. Results. Overall, 7 extracts showed in vitro antiplasmodial activity with IC50 ranging from 0.809 μg/mL to 5.886 μg/mL. The aqueous extract from the stem bark of T. mantaly (Tmsbw) was the most potent (IC50=0.809 μg/mL) and was further assessed for acute toxicity and efficacy in Plasmodium berghei-infected mice. Tmsbw was safe in mice with a median lethal dose (LD50) higher than 2000 mg/kg of body weight. It also exerted a good antimalarial efficacy in vivo with ED50 of 69.50 mg/kg and had no significant effect on biochemical, hematological, and histological parameters. Conclusion. The results suggest that the stem bark extract of T. mantaly possesses antimalarial activity.

scholarly journals α-Glucosidase inhibitory activities, functional properties, and safety of green tea polysaccharides as a potential source of dietary supplement

2018 ◽  
Vol 3 ◽  
pp. 124-132
Author(s):  
Ayse Karadag ◽  
Kubra Dogan ◽  
Ebru Pelvan ◽  
Arzu Tas ◽  
Ozlem Atli Eklioglu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Dietary Supplement ◽  
Green Tea ◽  
Lethal Dose ◽  
Monosaccharide Composition ◽  
Total Phenolic ◽  
Potential Health ◽  
Tea Polysaccharides ◽  
Mutagenic Effects

Procuring valuable bioactive compounds from low quality green tea is of great interest. Green tea polysaccharides (GTPS), obtained from low quality tea leaves, were examined for their yield, monosaccharide composition, total phenolic content, antioxidant potential, and structures as well as molecular weight distribution and in vitro anti-diabetic activities. GTPS were also evaluated for their toxicity by performing cytotoxicity and genotoxicity. Additionally, 50% lethal dose (LD50) for GTPS was determined using an acute toxicity test to assess the safe use of it as a dietary supplement. Monosaccharides of GTPS were mainly composed of xylose, glucose, ribose, galactose, arabinose, and glucuronic acid. GTPS exhibited 91.86% α-glucosidase inhibition at 0.5 mg/mL concentration. The α-glucosidase inhibitory activity of GTPS was higher than that of pharmacological glucosidase inhibitor, acarbose, which exhibited only 47.86% inhibition at 2.5 mg/mL concentration. Neither cytotoxic nor no mutagenic effects were found for GTPS. The acute toxicity also showed that LD50 of GTPS was greater than 5,000 mg/kg. The present work suggests that GTPS can be considered as an anti-diabetic dietary supplement without posing any potential health risk.

scholarly journals In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection

2016 ◽  
Vol 19 (2) ◽  
pp. 111 ◽  
Author(s):  
Asri Insiana Putri ◽  
Mohammad Na'iem ◽  
Sapto Indrioko ◽  
Sri Rahayu ◽  
Ari Indrianto
Keyword(s):  
Phenolic Compound ◽  
Total Phenolics ◽  
Culture Method ◽  
Dry Weight ◽  
Total Phenolic ◽  
Media Selection ◽  
In Vitro Screening ◽  
Tissue Culture Method ◽  
The Mean

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds fromtwo different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets invitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The meanof fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of freshweight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins andquantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attackedand un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. Itis notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminantsof pathogenecity. While the highest total phenolic compound were contained in gall rust extract(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use asmedia selection in vitro.

scholarly journals INVESTIGATION OF IN VITRO ANTIOXIDANT POTENTIAL OF METHANOLIC EXTRACT OF BARK OF PRUNUS CORNUTA AND ROOT OF RUMEX OBTUSIFOLIUS

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
M.C purohit ◽  
Navneet Singh ◽  
Goutam Kumar ◽  
Rashmi Rawat ◽  
Mahender Singh
Keyword(s):  
Antioxidant Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Bark Extract ◽  
Total Phenolic ◽  
Root Extract ◽  
Rumex Obtusifolius ◽  
Methanolic Extracts

In the present research work, in vitro, the antioxidant activity of methanolic extracts of bark of Prunus cornuta and root of Rumex obtusifolius, respectively, was assessed by DPPH free radical scavenging method. Total phenolic content (TPC) of methanolic extracts was also determined by spectrophotometric method using Folin-Ciocalteu reagent and calculated in ?g/mL gallic acid equivalent (GAE). DPPH scavenging activity results show that the root extract of Rumex obtusifolius (IC50 = 17.15) and bark extract of Prunus cornuta (IC50 = 27.32) shows good antioxidant potential. TPC of root extract was found to be 178.56 ?g/g GAE, and bark extract was 137.23 ?g/g GAE. Antioxidant effect (%) determined by using phosphomolybdate assay and superoxide anion radical scavenging percentage further supports the potential antioxidant activity.

Phytochemical screening, in vitro antioxidant and enzyme inhibitory proprieties and acute toxicity of extracts from the aerial parts of Ephedra nebrodensis, source of bioactive compounds

2021 ◽  
Vol 24 ◽  
Author(s):  
Meriem Hamoudi ◽  
Djouher Amroun ◽  
Sabrina Boutefnouchet ◽  
Chawki Bensouici ◽  
Samira Kaoula ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Ethyl Acetate Extract ◽  
Radical Scavenging ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Total Phenolic ◽  
Aerial Parts

Background: Due to the strong association between the chemistry of medicinal plants and their biological properties, it is important to determine their phytochemical composition to justify experimental tests. Objective: The aim of this study was to evaluate the in vitro antioxidant and the enzyme inhibitory properties and to identify the bioactive compounds present in the extracts of Ephedra nebrodensis growing in Algeria. Methods: Total phenolic and flavonoids content in these extracts were quantified by Folin-Ciocalteu and aluminum chloride methods. The antioxidant capacity was assessed using DPPH, ABTS, β-carotene/linoleic acid, CUPRAC and FRAP assays and in vitro cholinesterase activity against acetylcholinesterase and butyrylcholinesterase were evaluated. The chemical constituents of the extracts were analyzed by high-performance liquid chromatography coupled to mass spectrometric detection and gas chromatography coupled to mass spectrometric detection. For the acute toxicity study, extracts were administered to mice at single dose of 2 g/kg and 5 g/kg by gavage. Results: Plant extracts were rich in phenolic compounds. Ethyl acetate extract presented the highest phenolic (238.44 ± 1.50 µg GAE /mg of extract) and flavonoid (21.12 ± 0.00 µg QE /mg of extract) contents. Likewise, ethyl acetate extract showed potent radical scavenging and reducing properties. Ethanol: acetone extract showed inhibitory activity against acetylcholinesterase, and was a potent inhibitor of butyrylcholinesterase. In all extracts, flavonoids were the most abundant compounds. The phytochemical investigation showed the presence of alkaloids (ephedrine and pseudo-ephedrine). In the acute toxicity, the LD50 was superior to 5 g/kg body weight. There are not alterations in the histology of the liver and kidneys. Conclusion: This study demonstrated a good antioxidant potential and anticholinesterase activity of aerial parts of E. nebrodensis.

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