Induced Overexpression of Oct4A in Human Dental Pulp Cells Enhances Pluripotency and Multilineage Differentiation Capability

2015 ◽  
Vol 24 (8) ◽  
pp. 962-972 ◽  
Author(s):  
Lu Liu ◽  
Lijing Wu ◽  
Xi Wei ◽  
Junqi Ling
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Multilineage Differentiation ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

scholarly journals XPC Promotes Pluripotency of Human Dental Pulp Cells through Regulation of Oct-4/Sox2/c-Myc

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Lu Liu ◽  
Zhengjun Peng ◽  
Zhezhen Xu ◽  
Xi Wei
Keyword(s):  
Western Blot ◽  
Real Time ◽  
Real Time Pcr ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Multilineage Differentiation ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Introduction. Xeroderma pigmentosum group C (XPC), essential component of multisubunit stem cell coactivator complex (SCC), functions as the critical factor modulating pluripotency and genome integrity through interaction with Oct-4/Sox2. However, its specific role in regulating pluripotency and multilineage differentiation of human dental pulp cells (DPCs) remains unknown. Methods. To elucidate the functional role XPC played in pluripotency and multilineage differentiation of DPCs, expressions of XPC in DPCs with long-term culture were examined by real-time PCR and western blot. DPCs were transfected with lentiviral-mediated human XPC gene; then transfection rate was investigated by real-time PCR and western blot. Cell cycle, apoptosis, proliferation, senescence, multilineage differentiation, and expression of Oct-4/Sox2/c-Myc in transfected DPCs were examined. Results. XPC, Oct-4, Sox2, and c-Myc were downregulated at P7 compared with P3 in DPCs with long-term culture. XPC genes were upregulated in DPCs at P2 after transfection and maintained high expression level at P3 and P7. Cell proliferation, PI value, and telomerase activity were enhanced, whereas apoptosis was suppressed in transfected DPCs. Oct-4/Sox2/c-Myc were significantly upregulated, and multilineage differentiation in DPCs with XPC overexpression was enhanced after transfection. Conclusions. XPC plays an essential role in the modulation of pluripotency and multilineage differentiation of DPCs through regulation of Oct-4/Sox2/c-Myc.

scholarly journals The Preparation and Characterization of Chitosan-Based Hydrogels Cross-Linked by Glyoxal

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2449
Author(s):  
Beata Kaczmarek-Szczepańska ◽  
Olha Mazur ◽  
Marta Michalska-Sionkowska ◽  
Krzysztof Łukowicz ◽  
Anna Maria Osyczka
Keyword(s):  
Thermal Stability ◽  
Tannic Acid ◽  
Dental Pulp ◽  
Blood Compatibility ◽  
Dental Pulp Cells ◽  
Preliminary Assessment ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

In this study, hydrogels based on chitosan cross-linked by glyoxal have been investigated for potential medical applications. Hydrogels were loaded with tannic acid at different concentrations. The thermal stability and the polyphenol-releasing rate were determined. For a preliminary assessment of the clinical usefulness of the hydrogels, they were examined for blood compatibility and in the culture of human dental pulp cells (hDPC). The results showed that after immersion in a polyphenol solution, chitosan/glyoxal hydrogels remain nonhemolytic for erythrocytes, and we also did not observe the cytotoxic effect of hydrogels immersed in tannic acid (TA) solutions with different concentration. Tannic acid was successfully released from hydrogels, and its addition improved material thermal stability. Thus, the current findings open the possibility to consider such hydrogels in clinics.

Kallikrein Promotes Inflammation in Human Dental Pulp Cells Via Protease-Activated Receptor-1

2016 ◽  
Vol 117 (7) ◽  
pp. 1522-1528 ◽  
Author(s):  
Tomomi Hayama ◽  
Naoto Kamio ◽  
Tatsu Okabe ◽  
Koichiro Muromachi ◽  
Kiyoshi Matsushima
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Protease Activated Receptor 1

Effect of Nifedipine on the Differentiation of Human Dental Pulp Cells Cultured with Mineral Trioxide Aggregate

2013 ◽  
Vol 39 (6) ◽  
pp. 801-805 ◽  
Author(s):  
Su-Mi Woo ◽  
Yun-Chan Hwang ◽  
Hoi-Soon Lim ◽  
Nam-Ki Choi ◽  
Sun-Hun Kim ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Mineral Trioxide Aggregate ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells ◽  
Cells Cultured

In vitro culture of human dental pulp cells: some aspects of cells emerging early from the explant

1997 ◽  
Vol 1 (3) ◽  
pp. 131-140 ◽  
Author(s):  
L. Stanislawski ◽  
J. P. Carreau ◽  
M. Pouchelet ◽  
Z. H. J. Chen ◽  
M. Goldberg
Keyword(s):  
In Vitro Culture ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Dorsomorphin attenuates Jagged1‐induced mineralization in human dental pulp cells

2021 ◽  
Author(s):  
Jeeranan Manokawinchoke ◽  
Thiphon Watcharawipas ◽  
Kamoltham Ekmetipunth ◽  
Manoch Jiamjirachart ◽  
Thanaphum Osathanon
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells

Stimulating effects of quercetin and phenamil on differentiation of human dental pulp cells

2013 ◽  
Vol 121 (6) ◽  
pp. 559-565 ◽  
Author(s):  
Jong-Gil Kim ◽  
Kyung M. Son ◽  
Hee C. Park ◽  
Tingting Zhu ◽  
Ji H. Kwon ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Human Dental Pulp Cells ◽  
Human Dental Pulp ◽  
Pulp Cells
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