scholarly journals Epigallocatechin-3-gallate Attenuates Renal Damage by Suppressing Oxidative Stress in Diabetic db/db Mice

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Xiu Hong Yang ◽  
Yu Pan ◽  
Xiao Li Zhan ◽  
Bao Long Zhang ◽  
Li Li Guo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Green Tea ◽  
Renal Damage ◽  
Antioxidative Activity ◽  
Underlying Mechanism ◽  
Ang Ii ◽  
Pathological Changes ◽  
Egcg Treatment ◽  
Downstream Effector ◽  
P22 Phox

Epigallocatechin-3-gallate (EGCG), extracted from green tea, has been shown to have antioxidative activity. In the present study, we evaluated the effect of EGCG on the kidney function in db/db mice and also tried to investigate the underlying mechanism of the renoprotective effects of EGCG in both animals and cells. EGCG treatment could decrease the level of urinary protein, 8-iso-PGF2a, and Ang II. Moreover, EGCG could also change the level of several parameters associated with oxidative stress. In addition, the protein expression levels of AT-1R, p22-phox, p47-phox, p-ERK1/2, p-p38 MAPK, TGF-β1, andα-SMA in diabetic db/db mice were upregulated, and all of these symptoms were downregulated with the treatment of EGCG at 50 and 100 mg/kg/d. Furthermore, the pathological changes were ameliorated in db/db mice after EGCG treatment. HK-2 cell-based experiments indicated that EGCG could inhibit the expression of MAPK pathways, which is the downstream effector of Ang II mediated oxidative stress. All these results indicated that EGCG treatment could ameliorate changes of renal pathology and delay the progression of DKD by suppressing hyperglycemia-induced oxidative stress in diabetic db/db mice.

scholarly journals Green Tea Polyphenols for the Protection against Renal Damage Caused by Oxidative Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Takako Yokozawa ◽  
Jeong Sook Noh ◽  
Chan Hum Park
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Green Tea ◽  
Renal Damage ◽  
Large Fraction ◽  
Tea Polyphenols ◽  
Green Tea Polyphenols ◽  
Tea Polyphenol ◽  
Green Tea Polyphenol ◽  
Low Toxicity

Green tea, prepared from the leaves ofCamellia sinensisL., is a beverage that is popular worldwide. Polyphenols in green tea have been receiving much attention as potential compounds for the maintenance of human health due to their varied biological activity and low toxicity. In particular, the contribution of antioxidant activity to the prevention of diseases caused by oxidative stress has been focused upon. Therefore, in this study, we investigated the effects of (−)-epigallocatechin 3-O-gallate and (−)-epigallocatechin 3-O-gallate, which account for a large fraction of the components of green tea polyphenol, on oxidative stress-related renal disease. Our observations suggest that green tea polyphenols have a beneficial effect on pathological states related to oxidative stress of the kidney.

scholarly journals Alkaline Ceramidase Mediates the Oxidative Stress Response in Drosophila melanogaster Through Sphingosine

2019 ◽  
Vol 19 (3) ◽  
Author(s):  
Chun-Hong Zhang ◽  
Min-Jing Zhang ◽  
Xiao-Xiao Shi ◽  
Cungui Mao ◽  
Zeng-Rong Zhu
Keyword(s):  
Oxidative Stress ◽  
Drosophila Melanogaster ◽  
Stress Resistance ◽  
Antioxidative Activity ◽  
Underlying Mechanism ◽  
Oxidative Stress Response ◽  
Differentially Expressed Proteins ◽  
Wild Type ◽  
Transcription Level ◽  
Insight Into

Abstract Alkaline ceramidase (Dacer) in Drosophila melanogaster was demonstrated to be resistant to paraquat-induced oxidative stress. However, the underlying mechanism for this resistance remained unclear. Here, we showed that sphingosine feeding triggered the accumulation of hydrogen peroxide (H2O2). Dacer-deficient D. melanogaster (Dacer mutant) has higher catalase (CAT) activity and CAT transcription level, leading to higher resistance to oxidative stress induced by paraquat. By performing a quantitative proteomic analysis, we identified 79 differentially expressed proteins in comparing Dacer mutant to wild type. Three oxidoreductases, including two cytochrome P450 (CG3050, CG9438) and an oxoglutarate/iron-dependent dioxygenase (CG17807), were most significantly upregulated in Dacer mutant. We presumed that altered antioxidative activity in Dacer mutant might be responsible for increased oxidative stress resistance. Our work provides a novel insight into the oxidative antistress response in D. melanogaster.

Dibutyl phthalate-induced activation of ROS and ERK1/2 causes hepatic and renal damage in Kunming mice

2019 ◽  
Vol 38 (8) ◽  
pp. 938-950 ◽  
Author(s):  
L Cheng ◽  
J Li ◽  
J Cheng ◽  
Z Wu
Keyword(s):  
Oxidative Stress ◽  
Renal Damage ◽  
Dibutyl Phthalate ◽  
Underlying Mechanism ◽  
Renal Tissue ◽  
Oxygen Species ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
Km Mice

Dibutyl phthalate (DBP), a ubiquitous environmental contaminant, has been reported to be involved in hepatic and renal tissue damage. However, the role of DBP in oxidative stress and in extracellular signal-regulated kinase (ERK1/2) pathways remains unclear. To investigate the underlying mechanism, Kunming (KM) mice received daily doses of combinations of 50 mg/kg DBP, 50 mg/kg vitamin E (VitE), and 1 mg/kg PD98059 for 28 consecutive days. Any changes in reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as any histopathological alterations in tissues, were observed to assess oxidative stress. In addition, the levels of alanine aminotransferase, aspartate aminotransferase, and albumin in serum were used to evaluate liver function. The levels of creatinine and urea nitrogen in serum were measured to evaluate kidney function. We found that DBP significantly increased oxidative damage and the expression of phosphorylated ERK1/2. Furthermore, pretreatment with the ERK inhibitor PD98059 followed by the antioxidant VitE attenuated the levels of ROS, MDA, ERK1/2 phosphorylation, and DBP-mediated disorders, indicating that the oxidative stress and the ERK1/2 pathways are associated with DBP-induced hepatic and renal dysfunction in KM mice.

Black Tea-Induced Amelioration of Hepatic Oxidative Stress through Antioxidative Activity in EAC-Bearing Mice

2007 ◽  
Vol 26 (4) ◽  
pp. 245-254 ◽  
Author(s):  
Arindam Bhattacharyya ◽  
Debaprasad Mandal ◽  
Lakshmishri Lahiry ◽  
Sankar Bhattacharyya ◽  
Sreya Chattopadhyay ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidative Activity ◽  
Black Tea ◽  
Hepatic Oxidative Stress ◽  
Induced Amelioration

Antioxidative Activity of Laver Snack Prepared with Addition of Green Tea Extract

2017 ◽  
Vol 23 (4) ◽  
pp. 35-41
Author(s):  
Jeong Hee Park ◽  
Hang Yeon Jeong ◽  
Jeong Yong Cho ◽  
Jae Hak Moon
Keyword(s):  
Green Tea ◽  
Antioxidative Activity ◽  
Green Tea Extract ◽  
Tea Extract

Effect of Green Tea on Iron Status and Oxidative Stress in Iron-Loaded Rats

2008 ◽  
Vol 4 (4) ◽  
pp. 365-370 ◽  
Author(s):  
S. Ounjaijean ◽  
C. Thephinlap ◽  
U. Khansuwan ◽  
C. Phisalapong ◽  
S. Fucharoen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Green Tea ◽  
Iron Status ◽  
And Oxidative Stress

Testicular Injury Attenuated by Rapamycin Through Induction of Autophagy and Inhibition of Endoplasmic Reticulum Stress in Streptozotocin- Induced Diabetic Rats

2019 ◽  
Vol 19 (5) ◽  
pp. 665-675 ◽  
Author(s):  
Wenjiao Shi ◽  
Zhixin Guo ◽  
Ruixia Yuan
Keyword(s):  
Oxidative Stress ◽  
Endoplasmic Reticulum ◽  
Endoplasmic Reticulum Stress ◽  
Protective Effect ◽  
Er Stress ◽  
B Cell Lymphoma ◽  
Diabetic Rats ◽  
Pathological Changes ◽  
Rapamycin Treatment ◽  
Related Proteins

Background and Objective: This study investigated whether rapamycin has a protective effect on the testis of diabetic rats by regulating autophagy, endoplasmic reticulum stress, and oxidative stress. Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups: control, diabetic, and diabetic treated with rapamycin, which received gavage of rapamycin (2mg.kg-1.d-1) after induction of diabetes. Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65mg.Kg-1). All rats were sacrificed at the termination after 8 weeks of rapamycin treatment. The testicular pathological changes were determined by hematoxylin and eosin staining. The protein or mRNA expression of autophagy-related proteins (Beclin1, microtubule-associated protein light chain 3 (LC3), p62), ER stress marked proteins (CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP), caspase-12), oxidative stress-related proteins (p22phox, nuclear factor erythroid2-related factor 2 (Nrf2)) and apoptosis-related proteins (Bax, B cell lymphoma-2 (Bcl-2)) were assayed by western blot or real-time fluorescence quantitative PCR. Results: There were significant pathological changes in the testes of diabetic rats. The expression of Beclin1, LC3, Nrf2, Bcl-2 were significantly decreased and p62, CHOP, caspase12, p22phox, and Bax were notably increased in the testis of diabetic rats (P <0.05). However, rapamycin treatment for 8 weeks significantly reversed the above changes in the testis of diabetic rats (P <0.05). Conclusion: Rapamycin appears to produce a protective effect on the testes of diabetic rats by inducing the expression of autophagy and inhibiting the expression of ER-stress, oxidative stress, and apoptosis.

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