scholarly journals Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity ofDiospyros kakiFruit andCitrus unshiuPeel

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Gyo-Nam Kim ◽  
Mi-Rae Shin ◽  
Sung Ho Shin ◽  
Ah Reum Lee ◽  
Joo Young Lee ◽  
...  
Keyword(s):  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Dietary Fat ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Diospyros Kaki ◽  
Fat Absorption ◽  
Dietary Fat Absorption

Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme ofDiospyros kakifruit andCitrus unshiupeel mixture extract (PCM) was evaluatedin vitroand its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed micein vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC50value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p<0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.

UJI IN VITRO EKSTRAK AIR DAN ETANOL DARI BUAH ASAM GELUGUR, RIMPANG LENGKUAS, DAN KENCUR SEBAGAI INHIBITOR AKTIVITAS LIPASE PANKREAS

2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Dyah Iswantini ◽  
Latifah K. Darusman ◽  
Ana Fitriyani
Keyword(s):  
Body Weight ◽  
Pancreatic Lipase ◽  
Lipase Activity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Reduce Body Weight ◽  
Ethanol Extracts

Asam gelugur fruits of Garcinia often used to reduce body weight. Lengkuas and kencur are traditional herbal that potential for antiobesity because they could reduce the level of phospholipids, triglycerides, and cholesterol. The aim of the research was to evaluate the potencies of these herbal as antiobesity by measurement of their water and ethanol extracts capabilities as in vitro inhibitor of pancreatic lipase activity. The water and ethanol extracts of asam gelugur fruits contained saponins and alkaliods, respectively. The water extract of lengkuas rhizomes contained alkaloids, flavonoids, saponins, and quinones, while the ethanol extract contained alkaloids, flavonoids, saponins, and steroids. The water extract of kencur rhizomes contained saponins and quinones, while the ethanolextract contained alkaloids, flavonoids, steroids, and quinones. The highest inhibitory effect of all extracts was obtained from the ethanol extract of asam gelugur fruits with value of 86.3% at 150 ppm. The highest inhibitory effect of lengkuas extracts was from the ethanol extract at 200 ppm (56.2%). The highestinhibitory effect of kencur was showed by the ethanol extract with the value 37.6% at 300 ppm. These values were higher than the inhibitory effect of the positive control (Xenical®) at 100 ppm (10.6%).

scholarly journals In vivo hypotensive effect and in vitro inhibitory activity of some Cyperaceae species

2013 ◽  
Vol 49 (4) ◽  
pp. 803-809
Author(s):  
Monica Lacerda Lopes Martins ◽  
Henrique Poltronieri Pacheco ◽  
Iara Giuberti Perini ◽  
Dominik Lenz ◽  
Tadeu Uggere de Andrade ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Colorimetric Assay ◽  
Hypotensive Effect ◽  
Ace Inhibition ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Total Phenolic ◽  
Before And After

In 1820, French naturalist August Saint Hillaire, during a visit in Espírito Santo (ES), a state in southeastern Brazil, reported a popular use of Cyperaceae species as antidote to snake bites. The plant may even have a hypotensive effect, though it was never properly researched. The in vitro inhibitory of the angiotensin converting enzyme (ACE) activity of eigth ethanolic extracts of Cyperaceae was evaluated by colorimetric assay. Total phenolic and flavonoids were determined using colorimetric assay. The hypotensive effect of the active specie (Rhychonospora exaltata, ERE) and the in vivo ACE assay was measured in vivo using male Wistar Kyoto (ERE, 0.01-100mg/kg), with acetylcholine (ACh) as positive control (5 µg/kg, i.v.). The evaluation of ACE in vivo inhibitory effect was performed comparing the mean arterial pressure before and after ERE (10 mg/kg) in animals which received injection of angiotensin I (ANG I; 0,03, 03 and 300 µg/kg, i.v.). Captopril (30 mg/kg) was used as positive control. Bulbostylis capillaris (86.89 ± 15.20%) and ERE (74.89 ± 11.95%, ERE) were considered active in the in vitro ACE inhibition assay, at 100 µg/mL concentration. ACh lead to a hypotensive effect before and after ERE's curve (-40±5% and -41±3%). ERE showed a dose-dependent hypotensive effect and a in vivo ACE inhibitory effect. Cyperaceae species showed an inhibitory activity of ACE, in vitro, as well as high content of total phenolic and flavonoids. ERE exhibited an inhibitory effect on both in vitro and in vivo ACE. The selection of species used in popular medicine as antidotes, along with the in vitro assay of ACE inhibition, might be a biomonitoring method for the screening of new medicinal plants with hypotensive properties.

scholarly journals Apolipoprotein A-IV regulates chylomicron metabolism–mechanism and function

2012 ◽  
Vol 302 (6) ◽  
pp. G628-G636 ◽  
Author(s):  
Alison B. Kohan ◽  
Fei Wang ◽  
Xiaoming Li ◽  
Suzanne Bradshaw ◽  
Qing Yang ◽  
...  
Keyword(s):  
Dietary Fat ◽  
Cholesterol Metabolism ◽  
Plasma Lipid ◽  
Physiological Role ◽  
Lipid Absorption ◽  
Fat Absorption ◽  
Apolipoprotein A ◽  
And Function ◽  
Dietary Fat Absorption

Dietary fat is an important mediator of atherosclerosis and obesity. Despite its importance in mediating metabolic disease, there is still much unknown about dietary fat absorption in the intestine and especially the detailed biological roles of intestinal apolipoproteins involved in that process. We were specifically interested in determining the physiological role of the intestinal apolipoprotein A-IV (A-IV) using A-IV knockout (KO) mice. A-IV is stimulated by fat absorption in the intestine and is secreted on nascent chylomicrons into intestinal lymph. We found that A-IV KO mice had reduced plasma triglyceride (TG) and cholesterol levels and that this hypolipidemia persisted on a high-fat diet. A-IV KO did not cause abnormal intestinal lipid absorption, food intake, or adiposity. Additionally, A-IV KO did not cause abnormal liver TG and cholesterol metabolism, as assessed by measuring hepatic lipid content, lipogenic and cholesterol synthetic gene expression, and in vivo VLDL secretion. Instead, A-IV KO resulted in the secretion of larger chylomicrons from the intestine into the lymph, and those chylomicrons were cleared from the plasma more slowly than wild-type chylomicrons. These data suggest that A-IV has a previously unknown role in mediating the metabolism of chylomicrons, and therefore may be important in regulating plasma lipid metabolism.

scholarly journals Decreased Neonatal Dietary Fat Absorption and T Cell Cytotoxicity in Pancreatic Lipase-related Protein 2-Deficient Mice

1998 ◽  
Vol 273 (47) ◽  
pp. 31215-31221 ◽  
Author(s):  
Mark E. Lowe ◽  
Mark H. Kaplan ◽  
Laurie Jackson-Grusby ◽  
Dymphna D’Agostino ◽  
Michael J. Grusby
Keyword(s):  
T Cell ◽  
Pancreatic Lipase ◽  
Dietary Fat ◽  
Cell Cytotoxicity ◽  
Related Protein ◽  
Fat Absorption ◽  
Dietary Fat Absorption ◽  
Deficient Mice ◽  
T Cell Cytotoxicity

The Effect of Barbituric Acid Derivatives on Platelet Function in Vitro and in Vivo

1973 ◽  
Vol 30 (02) ◽  
pp. 315-326
Author(s):  
J. Heinz Joist ◽  
Jean-Pierre Cazenave ◽  
J. Fraser Mustard
Keyword(s):  
Platelet Aggregation ◽  
Platelet Function ◽  
Barbituric Acid ◽  
Platelet Rich Plasma ◽  
Inhibitory Effect ◽  
Primary Response ◽  
Sodium Pentobarbital ◽  
Barbituric Acid Derivatives

SummarySodium pentobarbital (SPB) and three other barbituric acid derivatives were found to inhibit platelet function in vitro. SPB had no effect on the primary response to ADP of platelets in platelet-rich plasma (PRP) or washed platelets but inhibited secondary aggregation induced by ADP in human PRP. The drug inhibited both phases of aggregation induced by epinephrine. SPB suppressed aggregation and the release reaction induced by collagen or low concentrations of thrombin, and platelet adherence to collagen-coated glass tubes. The inhibition by SPB of platelet aggregation was readily reversible and isotopically labeled SPB did not become firmly bound to platelets. No inhibitory effect on platelet aggregation induced by ADP, collagen, or thrombin could be detected in PRP obtained from rabbits after induction of SPB-anesthesia.

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