scholarly journals Fabrication and Evaluation of Polycaprolactone/Gelatin-Based Electrospun Nanofibers with Antibacterial Properties

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Lor Huai Chong ◽  
Mim Mim Lim ◽  
Naznin Sultana
Keyword(s):  
Drug Loading ◽  
Antibacterial Properties ◽  
Electrospun Nanofibers ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Electrospinning Technique ◽  
Water Contact ◽  
Nanofibrous Scaffolds ◽  
Model Drug

Nanofibrous scaffolds were fabricated through blending of a synthetic polymer, polycaprolactone (PCL), and a natural polymer, gelatin (GE), using an electrospinning technique. Processing and solution parameters were optimized to determine the suitable properties of PCL/GE-based nanofibers. Several characterizations were conducted to determine surface morphology by scanning electron microscopy (SEM), wettability using water contact angle measurement, and chemical bonding analysis using attenuated total reflectance (ATR) of PCL/GE-based nanofibers. Experimental results showed that 14% (w/v) PCL/GE with a flow rate of 0.5 mL/h and 18 kV demonstrated suitable properties. This nanofiber was then further investigated for itsin vitrodegradation, drug loading (using a model drug, tetracycline hydrochloride), and antibacterial testing (using zone inhibition method).

scholarly journals Electrospun Nanofibers of Polycaprolactone/Collagen as a Sustained-Release Drug Delivery System for Artemisinin

Pharmaceutics ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1228
Author(s):  
Peipei Huo ◽  
Xinxu Han ◽  
Wenyu Zhang ◽  
Jing Zhang ◽  
Parveen Kumar ◽  
...  
Keyword(s):  
Drug Release ◽  
Sustained Release ◽  
Electrospun Nanofibers ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Release Mechanism ◽  
Sustained Drug Release ◽  
Water Contact ◽  
Release Curve ◽  
Index Value

The application of artemisinin (ART) in the treatment of malaria has been restricted to a certain degree due to its inherent limitations, such as short half-life, poor solubility, limited bioavailability, and re-crystallization. Electrospun nanofibers loaded with ART provide an excellent solution to these limitations and yield sustained drug release as well as inhibition of drug re-crystallization. In this study, ART-loaded polycaprolactone (PCL)/collagen (Col) nanofibers with different proportions of polymers were prepared. ART-loaded PCL/Col nanofibers were characterized, and further ART anti-crystallization and release behaviors were studied. SEM was used to observe the morphology of PCL/Col nanofibers. X-ray diffraction (XRD) was used to characterize the physical state of ART in ART-loaded PCL/Col nanofibers. Fourier transform infrared spectroscopy (FTIR), water contact angle measurement, weight loss, degree of swelling, and drug release experiments can verify the differences in performance of ART-loaded PCL/Col nanofibers due to different polymer ratios. The release curve was analyzed by kinetics, showing sustained release for up to 48 h, and followed the Fickian release mechanism, which was shown by the diffusion index value obtained from the Korsmeyer-Peppas equation.

scholarly journals Simple method of fabrication of hydrophobic coatings for polyurethanes

2011 ◽  
Vol 9 (6) ◽  
pp. 1039-1045 ◽  
Author(s):  
Beata Butruk ◽  
Paulina Ziętek ◽  
Tomasz Ciach
Keyword(s):  
Contact Angle ◽  
High Efficiency ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Acoustic Microscopy ◽  
Water Contact ◽  
Simple Method ◽  
Hydrophobic Coatings ◽  
Grafting Reaction

AbstractThe aim of this study was to develop a method of manufacturing versatile hydrophobic coatings for polymers. Authors present a simple technique of polyurethane (PU) surface modification with covalently attached silicones (PDMS) or fluorocarbons (PFC). Diisocyanates were applied as linker molecules. The obtained coatings were characterized using spectroscopic analysis (FTIR), scanning acoustic microscopy (SAM) and water contact angle measurements. FTIR analysis revealed high efficiency of grafting reaction. The results of contact angle measurement indicated significant increase of hydrophobicity — from 66° (unmodified PU) to 113° (PU grafted with PDMS) and 118° (PU grafted with PFC). Acoustic microscopy analysis confirmed satisfactory homogeneity and smoothness of the fabricated layers. In vitro cell tests revealed non-adherent properties of the surfaces. Both, MTT assay and fluorescence staining confirmed non-cytotoxicity of the coatings, which makes them potential candidates for use in biomedical applications.

Electrospun acetaminophen-loaded cellulose acetate nanofibers fabricated using an epoxy-coated spinneret

e-Polymers ◽  
2015 ◽  
Vol 15 (5) ◽  
pp. 311-315 ◽  
Author(s):  
Xia Wang ◽  
Xiao-Yan Li ◽  
Ying Li ◽  
Hua Zou ◽  
Deng Guang Yu ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Cellulose Acetate ◽  
Amorphous State ◽  
Electrospun Nanofibers ◽  
Electrospinning Technique ◽  
Stainless Steel Capillary ◽  
Dissolution Tests ◽  
Model Drug ◽  
Functional Nanofibers

AbstractThis paper reports the investigation about the usage of an epoxy (EP)-coated spinneret for the preparation of medicated electrospun nanofibers. Cellulose acetate (CA) and acetaminophen (APAP) were used as the polymeric carrier and model drug, respectively. The electrospinning was undertaken using both EP-coated spinneret and traditional stainless steel capillary as spinnerets. According to the images from scanning electron microscopy, it is obvious that the nanofibers produced using the EP-coated spinneret had a finer diameter and a narrower size distribution (450±90 nm) than nanofibers fabricated using stainless steel equivalent (660±180 nm). In vitro dissolution tests revealed that the sustained-release profiles of nanofibers from the EP-coated spinneret were superior to those of their stainless steel equivalents, although APAP existed in a similar amorphous state in both nanofibers. Because the EP-coated material can exploit the electrical forces more effectively than its steel analogue, it can enhance the electrospinning technique for producing polymeric functional nanofibers.

Fabrication and Characterization of Copper-Chelated Polyethersulfone/Polydopamine Microfiltration Membrane for Antifouling Applications

2021 ◽  
Vol 878 ◽  
pp. 23-27
Author(s):  
Xech Rafael Aldrei U. Dela Paz ◽  
Rhoda B. Leron
Keyword(s):  
Cell Structure ◽  
Pure Water ◽  
Water Flux ◽  
Antibacterial Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Water Contact ◽  
Hydrophilic Character ◽  
Pure Water Flux ◽  
Pes Membrane

In this work, we investigated the applicability of polyethersulfone/polydopamine/copper (II) (PES/PDA/Cu2+) membrane composite in microfiltration. Pristine PES membrane was prepared via non-induced phase separation (NIPS), and the surface was modified by coating with PDA and deposition of Cu (II) to enhance its physical and antibacterial properties. The membrane was characterized using energy-dispersive X-ray spectroscopy, scanning electron microscopy and water contact angle measurement. The hydrophilic character of the PES membrane significantly increased and the mechanical properties were greatly improved. Results showed that the porosity of the membrane was affected by the concentration of the polymer in the casting solution, and the membrane was suitable for filtration application due to its open-cell structure. Pure water flux was enhanced upon surface modification implying that hydrophilicity has more influence on the flux than the shrinking of the pore size. The antibacterial assay suggested that the membranes possess antibacterial property.

scholarly journals Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells

Materials ◽  
2019 ◽  
Vol 12 (21) ◽  
pp. 3583 ◽  
Author(s):  
Martina Doubkova ◽  
Ivana Nemcakova ◽  
Ivan Jirka ◽  
Vitezslav Brezina ◽  
Lucie Bacakova
Keyword(s):  
Photoelectron Spectroscopy ◽  
Chemical Properties ◽  
In Vitro Study ◽  
Ammonium Hydroxide ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Orthopaedic Implants ◽  
Water Contact ◽  
Physico Chemical

Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.

scholarly journals Superhydrophobic functionalized cellulosic paper by copper hydroxide nanorods for oils purification

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ahmed S. Belal ◽  
Jehan El Nady ◽  
Azza Shokry ◽  
Shaker Ebrahim ◽  
Moataz Soliman ◽  
...  
Keyword(s):  
Contact Angle ◽  
Filter Paper ◽  
Separation Efficiency ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Absorption Capacity ◽  
Morphological Properties ◽  
Copper Hydroxide ◽  
Immersion Method ◽  
Water Contact

AbstractOily water contamination has been sighted as one of the most global environmental pollution. Herein, copper hydroxide nanorods layer was constructed onto cellulosic filter paper surface cured with polydopamine, Ag nanoparticles, and Cu NPs through immersion method. This work has been aimed to produce a superhydrophobic and superoleophilic cellulosic filter paper. The structure, crystalline, and morphological properties of these modified cellulosic filter paper were investigated. Scanning electron microscope images confirmed that the modified surface was rougher compared with the pristine surface. The contact angle measurement confirmed the hydrophobic nature of these modified surfaces with a water contact angle of 169.7°. The absorption capacity was 8.2 g/g for diesel oil and the separation efficiency was higher than 99%. It was noted that the flux in the case of low viscosity solvent as n-hexane was 9663.5 Lm−2 h−1, while for the viscous oil as diesel was 1452.7 Lm−2 h−1.

Tailoring the in vitro characteristics of poly(vinyl alcohol)-nanohydroxyapatite composite scaffolds for bone tissue engineering

2016 ◽  
Vol 36 (8) ◽  
pp. 771-784 ◽  
Author(s):  
Tejinder Kaur ◽  
Arunachalam Thirugnanam ◽  
Krishna Pramanik
Keyword(s):  
Mechanical Strength ◽  
Vinyl Alcohol ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Poly Vinyl Alcohol ◽  
Apatite Formation ◽  
Composite Scaffolds ◽  
Alizarin Red ◽  
Casting Technique

Abstract Poly(vinyl alcohol) reinforced with nanohydroxyapatite (PVA-nHA) composite scaffolds were developed by varying the nHA (1%, 2%, 3%, 4%, and 5%, w/v) composition in the PVA matrix by solvent casting technique. The developed composite scaffolds were characterized using scanning electron microscopy (SEM), X-ray powder diffraction (XRD), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, and contact angle measurement. The stability of the composite scaffolds in physiological environment was evaluated by swelling and degradation studies. Further, these composite scaffolds were tested for in vitro bioactivity, hemolysis, biocompatibility, and mechanical strength. SEM micrographs showed a homogenous distribution of nHA (3%, w/v) in the PVA matrix. XRD and ATR-FTIR analysis confirmed no phase contamination and the existence of the chemical bond between PVA-nHA at approximately 2474 cm-1. PVA-nHA composite scaffolds with 3% (w/v) concentration of nHA showed nominal swelling and degradation behavior with good mechanical strength. The mechanical strength and degradation properties of the scaffold above 3% (w/v) of nHA was found to deteriorate, which is due to the agglomeration of nHA. The in vitro bioactivity and hemolysis studies showed improved apatite formation and hemocompatibility of the developed scaffolds. In vitro cell adhesion, proliferation, alkaline phosphatase activity, and Alizarin red S staining confirmed the biocompatibility of the composite scaffolds.

Bioresorbable electrospun nanofibrous scaffolds loaded with bioactive molecules

e-Polymers ◽  
2009 ◽  
Vol 9 (1) ◽  
Author(s):  
Chiara Gualandi ◽  
Piotr Wilczek ◽  
Maria Letizia Focarete ◽  
Gianandrea Pasquinelli ◽  
Michal Kawalec ◽  
...  
Keyword(s):  
Mononuclear Cells ◽  
Degradation Kinetics ◽  
Hydrolytic Degradation ◽  
Mesenchymal Cells ◽  
Bioactive Molecules ◽  
Differential Interference Contrast Microscopy ◽  
Electrospinning Technique ◽  
Ki 67 ◽  
Nanofibrous Scaffolds

AbstractElectrospinning technology is used to fabricate sub-micrometric fiber mats made of a random equimolar poly(lactide-co-glycolide) copolymer (PLGA), whose in vitro hydrolytic degradation kinetics is investigated over a period of 49 days in phosphate buffer at 37 °C. The PLGA mats show a decrease of molecular weight (by GPC) from the very beginning of the experiment, whereas a macroscopic weight loss from the samples is appreciated (by gravimetry) only after 20 days of buffer exposure. The molar mass distribution curves remain monomodal during the degradation experiment suggesting that no acid auto-catalyzed hydrolysis, commonly observed in bulk specimens, occurs in sub-micrometric PLGA fibers. PLGA scaffolds containing Endothelial Growth Factor Supplement (ECGS) were also fabricated by electrospinning, from ECGS-containing polymer solutions. Mesenchymal cells derived from human bone marrow mononuclear cells were cultured in the presence of such ECGS-loaded PLGA scaffolds. Flow cytometry and Differential Interference Contrast microscopy were used to characterize the cell cultures over a 7 day period. The results of AnexinV/PI staining and of intranuclear Ki-67 protein expression show, together with concomitant cell morphology modifications, that growth factors released from the scaffolds support the survival, proliferation and growth of the mesenchymal cells. This result demonstrates that ECGS maintains its bioactivity upon release from the electrospun fibers and shows the versatility of the electrospinning technique.

Plasma Pre-Treatment of Polyethylene Terephthalate Substrate Influence on the Properties of ZnO Thin Film

2014 ◽  
Vol 895 ◽  
pp. 41-44
Author(s):  
Seiw Yen Tho ◽  
Kamarulazizi Ibrahim
Keyword(s):  
Thin Film ◽  
Thin Films ◽  
Polyethylene Terephthalate ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Zno Thin Films ◽  
Zno Thin Film ◽  
Water Contact ◽  
Pre Treatment ◽  
Pet Substrate

In this work, the influences of plasma pre-treatment on polyethylene terephthalate (PET) substrate to the properties of ZnO thin film have been carried out. ZnO thin films were successfully grown on PET substrate by spin coating method. In order to study the effects of plasma pre-treatment, a comparison of treated and untreated condition was employed. Water contact angle measurement had been carried out for PET wettability study prior to ZnO thin film coating. Morphology study of ZnO thin film was performed by scanning probe microscope (SPM). Besides, optical study of the ZnO thin film was done by using UV-vis spectrophotometer. All the measured results show that plasma pre-treatment of PET substrate plays an important role in enhancing the wettability of PET and optical properties of the ZnO thin films. In conclusion, pre-treatment of PET surface is essential to produce higher quality ZnO thin film on this particular substrate in which would pave the way for the integration of future devices.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

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