scholarly journals Isolation and Characterization of Human Lung Lymphatic Endothelial Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Bruno Lorusso ◽  
Angela Falco ◽  
Denise Madeddu ◽  
Caterina Frati ◽  
Stefano Cavalli ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tyrosine Kinases ◽  
Human Lung ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization ◽  
Microbial Agents ◽  
Diseased Lung

Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retainin vitroseveral receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.

scholarly journals P3.01-050 Isolation and Characterization of Lymphatic Endothelial Cells from Neoplastic and Normal Human Lung

2017 ◽  
Vol 12 (1) ◽  
pp. S1150
Author(s):  
Federico Quaini ◽  
Bruno Lorusso ◽  
Angela Falco ◽  
Denise Madeddu ◽  
Costanza Lagrasta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization ◽  
Normal Human

Isolation and characterization of primary lymphatic endothelial cells from rat thoracic duct

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Hideki Ohta ◽  
Kenichi Katsube ◽  
Jyun‐ichi Ogawa ◽  
Masaki Yanagishita
Keyword(s):  
Endothelial Cells ◽  
Thoracic Duct ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization

scholarly journals Liprin β1 is highly expressed in lymphatic vasculature and is important for lymphatic vessel integrity

Blood ◽  
2010 ◽  
Vol 115 (4) ◽  
pp. 906-909 ◽  
Author(s):  
Camilla Norrmén ◽  
Wouter Vandevelde ◽  
Annelii Ny ◽  
Pipsa Saharinen ◽  
Massimiliano Gentile ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Vessel ◽  
Lipid Absorption ◽  
Tissue Fluid ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization ◽  
Lymphatic Vasculature ◽  
Vessel Integrity

Abstract The lymphatic vasculature is important for the regulation of tissue fluid homeostasis, immune response, and lipid absorption, and the development of in vitro models should allow for a better understanding of the mechanisms regulating lymphatic vascular growth, repair, and function. Here we report isolation and characterization of lymphatic endothelial cells from human intestine and show that intestinal lymphatic endothelial cells have a related but distinct gene expression profile from human dermal lymphatic endothelial cells. Furthermore, we identify liprin β1, a member of the family of LAR transmembrane tyrosine phosphatase-interacting proteins, as highly expressed in intestinal lymphatic endothelial cells in vitro and lymphatic vasculature in vivo, and show that it plays an important role in the maintenance of lymphatic vessel integrity in Xenopus tadpoles.

Isolation and characterization of rabbit cardiac endothelial cells: Response to cyclic strain and growth factors in vitro

1992 ◽  
Vol 53 (4) ◽  
pp. 331-334 ◽  
Author(s):  
Mark D. Widmann ◽  
George V. Letsou ◽  
Stacie Phan ◽  
John C. Baldwin ◽  
Bauer E. Sumpio
Keyword(s):  
Endothelial Cells ◽  
Growth Factors ◽  
Cyclic Strain ◽  
Isolation And Characterization

Intrapericardial lymphangioma with podoplanin immunohistochemical characterization of lymphatic endothelial cells

2000 ◽  
Vol 37 (1) ◽  
pp. 85-95 ◽  
Author(s):  
E Sinzelle ◽  
J P Duong Van Huyen ◽  
S Breiteneder-Geleff ◽  
E Braunberger ◽  
A Deloche ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Endothelial Cells
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