scholarly journals P2‐3: Isolation and characterization of lymphatic endothelial cells from lung tissues affected by lymphangioleiomyomatosis

Respirology ◽  
2021 ◽  
Vol 26 (S3) ◽  
pp. 113-114
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization

scholarly journals P3.01-050 Isolation and Characterization of Lymphatic Endothelial Cells from Neoplastic and Normal Human Lung

2017 ◽  
Vol 12 (1) ◽  
pp. S1150
Author(s):  
Federico Quaini ◽  
Bruno Lorusso ◽  
Angela Falco ◽  
Denise Madeddu ◽  
Costanza Lagrasta ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization ◽  
Normal Human

Isolation and characterization of primary lymphatic endothelial cells from rat thoracic duct

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Hideki Ohta ◽  
Kenichi Katsube ◽  
Jyun‐ichi Ogawa ◽  
Masaki Yanagishita
Keyword(s):  
Endothelial Cells ◽  
Thoracic Duct ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Human Lung Lymphatic Endothelial Cells

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Bruno Lorusso ◽  
Angela Falco ◽  
Denise Madeddu ◽  
Caterina Frati ◽  
Stefano Cavalli ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tyrosine Kinases ◽  
Human Lung ◽  
Lymphatic Endothelial Cells ◽  
Isolation And Characterization ◽  
Microbial Agents ◽  
Diseased Lung

Characterization of lymphatic endothelial cells from the respiratory system may be crucial to investigate the role of the lymphatic system in the normal and diseased lung. We describe a simple and inexpensive method to harvest, isolate, and expand lymphatic endothelial cells from the human lung (HL-LECs). Fifty-five samples of healthy lung selected from patients undergoing lobectomy were studied. A two-step purification tool, based on paramagnetic sorting with monoclonal antibodies to CD31 and Podoplanin, was employed to select a pure population of HL-LECs. The purity of HL-LECs was assessed by morphologic criteria, immunocytochemistry, flow cytometry, and functional assays. Interestingly, these cells retainin vitroseveral receptor tyrosine kinases (RTKs) implicated in cell survival and proliferation. HL-LECs represent a clinically relevant cellular substrate to study lymphatic biology, lymphoangiogenesis, interaction with microbial agents, wound healing, and anticancer therapy.

Intrapericardial lymphangioma with podoplanin immunohistochemical characterization of lymphatic endothelial cells

2000 ◽  
Vol 37 (1) ◽  
pp. 85-95 ◽  
Author(s):  
E Sinzelle ◽  
J P Duong Van Huyen ◽  
S Breiteneder-Geleff ◽  
E Braunberger ◽  
A Deloche ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Endothelial Cells

scholarly journals Characterization of the mechanisms involved in the early specification and migration of Prox1-expressing lymphatic endothelial cells

2011 ◽  
Vol 356 (1) ◽  
pp. 171
Author(s):  
Ying Yang ◽  
Kimberle Shen ◽  
Sathish Srinivasan ◽  
Amira Masri ◽  
Joshua Scallan ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Lymphatic Endothelial Cells ◽  
And Migration

Isolation and Characterization of Brain Endothelial Cells: Morphology and Enzyme Activity

1980 ◽  
Vol 35 (2) ◽  
pp. 374-381 ◽  
Author(s):  
Stuart K. Williams ◽  
John F. Gillis ◽  
Maureen A. Matthews† ◽  
Roger C. Wagnert ◽  
Mark W. Bitensky
Keyword(s):  
Endothelial Cells ◽  
Enzyme Activity ◽  
Brain Endothelial Cells ◽  
Isolation And Characterization
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