scholarly journals Composition and Antimicrobial Activity ofEuphrasia rostkovianaHayne Essential Oil

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Pavel Novy ◽  
Hana Davidova ◽  
Cecilia Suqued Serrano-Rojero ◽  
Johana Rondevaldova ◽  
Josef Pulkrabek ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacterial Infections ◽  
Antimicrobial Effect ◽  
Main Constituent ◽  
Hexadecanoic Acid ◽  
Eye Infections ◽  
Gram Positive Bacteria ◽  
Health Disorders

Eyebright,Euphrasia rostkovianaHayne (Scrophulariaceae), is a medicinal plant traditionally used in Europe for the treatment of various health disorders, especially as eyewash to treat eye ailments such as conjunctivitis and blepharitis that can be associated with bacterial infections. SomeEuphrasiaspecies have been previously reported to contain essential oil. However, the composition and bioactivity ofE. rostkovianaoil are unknown. Therefore, in this study, we investigated the chemical composition and antimicrobial activity of the eyebright essential oil against some organisms associated with eye infections:Enterococcus faecalis,Escherichia coli,Klebsiella pneumoniae,Staphylococcus aureus,S. epidermidis,Pseudomonas aeruginosa, andCandida albicans. GC-MS analysis revealed more than 70 constituents, with n-hexadecanoic acid (18.47%) as the main constituent followed by thymol (7.97%), myristic acid (4.71%), linalool (4.65%), and anethole (4.09%). The essential oil showed antimicrobial effect against all organisms tested with the exception ofP. aeruginosa. The best activity was observed against all Gram-positive bacteria tested with the minimum inhibitory concentrations of 512 µg/mL. This is the first report on the chemical composition ofE. rostkovianaessential oil and its antimicrobial activity.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oils of Leaves, Roots and Bark of Glossocalyx staudtii

2009 ◽  
Vol 4 (8) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Huguette Agnaniet ◽  
Thomas Makani ◽  
Raphaël Bikanga ◽  
Louis Clément Obame ◽  
Jacques Lebibi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Disc Diffusion ◽  
Aliphatic Compounds ◽  
Gram Positive Bacteria ◽  
Agar Disc ◽  
Leaf Oil ◽  
Main Component

The chemical composition of the essential oils obtained by hydrodistillation from air dried leaves, bark and roots of Glossocalyx staudtii Engl. grown in Gabon was analyzed by GC and GC-MS. The essential oil content was 0.84% (w/w), 0.28% (w/w), and 0.74% (w/w), respectively. The leaf oil was characterized by a high content of monoterpene hydrocarbons (64.8%), with β-pinene (30.6%) and α-pinene (22.6%) as the major constituents. The oil obtained from the bark contained 30.5% of oxygenated monoterpenes with cryptone as the main constituent (11.5%) and 28.9% of aliphatic compounds, with 9.3% of 2-tridecanone; in the roots oil, the main component was 2-tridecanone (55.2%). The antimicrobial activity of the oils was studied by means of the agar disc diffusion and broth microdilution methods. The Gram positive bacteria were the most sensitive to the essential oils. A significant anticandidal effect of the bark essential oil was observed.

Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

2020 ◽  
Author(s):  
Haïfa Debbabi ◽  
Ridha El Mokni ◽  
Ibrahim Jlassi ◽  
Rajesh K Joshi ◽  
Saoussen Hammami
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

scholarly journals Chemical composition and antimicrobial activity of essential oil from Pyrethrum pulchrum Ledeb.

2018 ◽  
Vol 19 (45) ◽  
pp. 38-43 ◽  
Author(s):  
Uugangerel Erdenetsogt ◽  
Choijamts Gotov ◽  
Kerstin Voigt ◽  
Stefan Bartram ◽  
Wilhelm Boland ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Diffusion Method ◽  
Oil Composition ◽  
Disk Diffusion Method ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Total Oil ◽  
Sporidiobolus Salmonicolor

The chemical composition and antimicrobial activity of the essential oil from the aerial parts of Pyrethrum pulchrum Ledeb. were investigated. Dried plant material was hydro-distillated yielding 0.1% of essential oil. The oil was analyzed by GC-MS techniques. Fifty-five compounds were identified representing 99.7% of the total oil composition. Camphor was the predominant compound (33.9%) followed by linalool (21.1%) and α-pinene (9.0%). The antimicrobial activity of the oil was determined using the disk diffusion method against Gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus and Enterococcus faecalis), Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli), Mycobacterium vaccae and fungi (Candida albicans, Sporidiobolus salmonicolor and Penicillum notatum). The essential oil of P. pulchrum displays an intermediate activity against selected bacteria.

scholarly journals Chemical composition, antioxidative and antimicrobial activity of essential oil Ocimum sanctum L.

2013 ◽  
Vol 67 (3) ◽  
pp. 427-435 ◽  
Author(s):  
Damir Beatovic ◽  
Slavica Jelacic ◽  
Cedo Oparnica ◽  
Dijana Krstic-Milosevic ◽  
Jasmina Glamoclija ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Raw Material ◽  
Aspergillus Ochraceus ◽  
Ocimum Sanctum ◽  
Gram Negative ◽  
Predominant Component ◽  
Ocimum Tenuiflorum

Ocimum sanctum L. (Lamiaceae) sin. Ocimum tenuiflorum L. or Tulsi basil is a plant originating from tropical and subtropical areas of India. It is used in both the traditional and official medicine in India. Tulsi is a type of basil that is insufficiently explored and studied in Europe. The goal of this paper is to determine the chemical composition, antioxidative, and antimicrobial activity of the essential oil Ocimum sanctum L. grown in Serbia. The quantity of essential oil in 100 g of herb (v/w) is 0.68%, with 41 components identified in the tested essential oil. The most represented chemical group are sesquiturpene hydrocarbonates with 80.47%. Other groups were much less represented. Sesquiturpene hydrocarbonate ?-cariophyllene is a predominant component in the essential oil with 63.80%. The quantity of tested essential oil needed to achieve 50% of inhibition of DPPH radicals is 0.35 ?g/ml, and it has high potential to neutralize free radicals. The essential oil exhibited antibacterial activity to all tested strains of bacteria, both Gram-positive and Gram-negative. It affected all strains in an inhibitory way in the interval 0.34-41.50 ?l/ml, and in a bactericide way within the range 22.50-124.5 ?l/ml. The most sensitive strains of bacteria are Salmonella typhimurium and Escherichia coli, while Listeria monocytogenes and Enterococus faecalis showed greatest resistance. The essential oil exhibited antifugal activity on all tested fungi. It affected all tested fungi in an inhibitory way in the interval 4.42-8.83 ?l/ml, and in a microbicide way within the range 10.00-50.00 ?l/ml. The most sensitive fungi are: Aspergillus ochraceus, Penicillium ochrochloron and Penicilium funiculosum, while the most resistent one is Aspergillus niger. The tested basil essential oil Ocimum sanctum demonstrated significant antioxidative and antimicrobial effect and may be used as a raw material in food, pharmaceutical and chemical industries.

scholarly journals Chemical Composition and Antimicrobial Activity of the Essential Oil from Aerial Parts of Algerian Pulicaria Mauritanica

2016 ◽  
Vol 11 (1) ◽  
pp. 1934578X1601100 ◽  
Author(s):  
Mohammed Gherib ◽  
Chahrazed Bekhechi ◽  
Fewzia Atik Bekkara ◽  
Ange Bighelli ◽  
Joseph Casanova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Filamentous Fungi ◽  
Antimicrobial Effect ◽  
Similar Composition ◽  
Aerial Parts ◽  
Western Algeria ◽  
Flowering Periods ◽  
C Nmr

One oil sample isolated from aerial parts of Pulicaria mauritanica Coss. from Western Algeria has been analyzed by GC(RI), GC-MS and 13C NMR. In total, 21 components, accounting for 97.0% of the oil, were identified. Then, 36 oil samples coming from plants harvested at two flowering periods in three locations were analyzed by GC(RI) and 13C NMR. Although all the oil samples exhibited similar composition, dominated by carvotanacetone (89.2–96.1%), the yield of essential oil varied drastically from sample to sample (0.35–1.44%), depending on the location of harvest. The essential oil displayed moderate antimicrobial effect against bacteria, yeast and filamentous fungi (MIC = 2–4 μL/mL).

scholarly journals Essential Oil Composition and Antimicrobial Activity of Ballota nigra L. ssp Foetida

2009 ◽  
Vol 4 (4) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Daniele Fraternale ◽  
Anahi Bucchini ◽  
Laura Giamperi ◽  
Donata Ricci
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oil Composition ◽  
Caryophyllene Oxide ◽  
Oil Composition ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Aerial Parts ◽  
Germacrene D

The chemical composition of the essential oil of Ballota nigra L. ssp foetida obtained from the flowering aerial parts was analyzed by GC/MS. From the 37 identified constituents of the oil, β-caryophyllene (20.0%), germacrene D (18.0%) and caryophyllene oxide (15.0%) were the major components. The oil was active against both Gram-negative and Gram-positive bacteria as well as against three Candida species.

scholarly journals Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity

2010 ◽  
Vol 75 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mariana Pavel ◽  
Mihailo Ristic ◽  
Tatjana Stevic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antimicrobial Properties ◽  
Herbal Drug ◽  
Qualitative And Quantitative Analysis ◽  
Gram Positive Bacteria ◽  
Main Components

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), ?-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and ?-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 ?l/ml.

scholarly journals Chemical composition, antimicrobial and antioxidant activity of the essential oil of leaves of Eugenia involucrata DC.

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Adrieli Gorlin Toledo ◽  
Juliete Gomes de Lara de Souza ◽  
Jéssica Patrícia Borges da Silva ◽  
Wagner Alex Jann Favreto ◽  
Willian Ferreira da Costa ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antioxidant Potential ◽  
Gram Positive Bacteria ◽  
Dpph Method ◽  
Germacrene D ◽  
Antimicrobial And Antioxidant Activity ◽  
Reduction Capacity

In the Myrtaceae family, the species Eugenia involucrata DC., popularly known as "cerejeira-do-mato", is traditionally used for the antidiarrheal and digestive action of its leaves. However, no studies were found in the literature regarding its antimicrobial and antioxidant potential. In this context, the objective of the present study was to determine the chemical composition by gas chromatography coupled to mass spectrometry (GC-MS) to evaluate the antimicrobial activity by the broth microdilution technique and the antioxidant activity by the 2,2-diphenyl-1-picryl-hydrazila (DPPH) method of the essential oil of E. involucrata leaves. GC-MS identified 28 compounds, all sesquiterpenes, corresponding to 89.41% of the essential oil. The antimicrobial activity of the essential oil was observed for all Gram-positive bacteria tested (Staplylococcus epidermidis, Enterococcus faecalis, Bacillus subtilis and Staplylococcus aureus) and for yeast Candida albicans. The essential oil presented a reduction capacity of DPPH up to 66.81%, evidencing its antioxidant potential. It is suggested that the antimicrobial and antioxidant action of E. involucrata essential oil is related to the presence of the major compounds, elixene (26.53%), β-caryophyllene (13.16%), α-copaene (8.41%) and germacrene D (7.17%).

scholarly journals Chemical Composition and in vitro Antibacterial Activity of the Essential Oil of Verbesina negrensis from the Venezuelan Andes

2015 ◽  
Vol 10 (7) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Flor D. Mora ◽  
Yesenia L. Rojas ◽  
Viviana González ◽  
Judith Velasco ◽  
Tulia Díaz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Enterococcus Faecalis ◽  
In Vitro Activity ◽  
Gram Positive Bacteria ◽  
In Vitro Antibacterial Activity

The composition and antimicrobial activity of the essential oil from Verbesina negrensis Steyerm. leaves is reported. Analysis was performed by GC/MS. Major constituents were α-pinene (43.1%), α-humulene (13.8%), δ-cadinene (8.1%), limonene (4.6%) and bicyclogermacrene (4.2%). The essential oil showed in vitro activity against the Gram-positive bacteria Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212 (MIC 500 and 350 μL/mL respectively).

scholarly journals Antibacterial Activity and Chemical Composition of Essential Oils from Some Galium (Rubiaceae) Species Against Pathogenic Bacteria

2017 ◽  
Vol 5 (11) ◽  
pp. 1330
Author(s):  
Filiz Yağız ◽  
Rifat Battaloğlu ◽  
Sedef İlk ◽  
Ahmet Savran
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Inhibitory Effect ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Potent Inhibitory Effect

In this work, chemical composition and antimicrobial activity of the essential oils of Galium incanum, Galium dieckii ve Galium aladaghense were firstly reported. The essential oils were obtained from the all parts of the plant by hydrodistillation and analyzed by using GC-MS. Antimicrobial activity of synthezied essential oils was carried out against 5 pathogen bacteria Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas syringae pv. tomato (P. syringae) DC300, Salmonella enterica serotype Typhmurium (S. typhmurium) SL 1344 and Streptococcus mutans (S. mutans) ATCC 25175. According to the results, it was determined that isolated essential oils comprised of 61 compounds. Compounds of essential oils included that structure monoterpene (8.2%), monoterpenoid (14.75%) and sesquiterpene (14.75%). Unclassified compounds have been identified as other compounds. From the antimicrobial activity was observed that the isolated essential oil from Galium incanum, Galium dieckii ve Galium aladaghense exhibited a potent inhibitory effect against all gram negative and gram positive bacteria with diameter of inhibition zones ranging from 4.3 to 12.3 mm. Essential oil of Galium aladaghense indicated that high antimicrobial activity on all bacteria than Galium incanum and Galium dieckii.

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