scholarly journals Effects of “Danzhi Decoction” on Chronic Pelvic Pain, Hemodynamics, and Proinflammatory Factors in the Murine Model of Sequelae of Pelvic Inflammatory Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Xiaoling Bu ◽  
Yanxia Liu ◽  
Qiudan Lu ◽  
Zhe Jin
Keyword(s):  
Pelvic Pain ◽  
Murine Model ◽  
Genital Tract ◽  
Chronic Pelvic Pain ◽  
Inflammatory Diseases ◽  
Blood Stasis ◽  
High Dose ◽  
Positive Role ◽  
Blood Flows ◽  
Proinflammatory Factors

Objective.To evaluate the effect of Danzhi decoction (DZD) on chronic pelvic pain (CPP), hemodynamics, and proinflammatory factors of sequelae of pelvic inflammatory diseases (SPID) in murine model.Methods.SPID mice were randomly treated with high-dose DZD, mid-dose DZD, low-dose DZD, aspirin, and vehicle for 3 estrous circles. The Mouse Grimace Scale (MGS) was performed to evaluate CPP; blood flows of the upper genital tract, pelvic wall, and mesentery were used to assess hemodynamics in SPID mice; expressions of vascular endothelial growth factor (VEGF), angiopoietin-2 (Ang-2), and osteopontin (OPN) were measured by Western blot and immunochemistry.Results.Treatment with dose-dependent DZD significantly decreased the MGS scores, accelerated blood flows of the pelvis, and reduced expressions of VEGF, Ang-2, and OPN in the upper genital tract.Conclusions and Discussions.DZD was effective in relieving CPP and improving hemodynamics of the pelvic blood-stasis microenvironment in SPID mice. There was a relationship between CPP and the pelvic blood-stasis microenvironment. Furthermore, DZD might play a positive role in the anti-inflammatory process.

scholarly journals A novel murine model of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) induced by immunization with a spermine binding protein (p25) peptide

2013 ◽  
Vol 304 (6) ◽  
pp. R415-R422 ◽  
Author(s):  
Cengiz Z. Altuntas ◽  
Firouz Daneshgari ◽  
Elias Veizi ◽  
Kenan Izgi ◽  
Fuat Bicer ◽  
...  
Keyword(s):  
Pelvic Pain ◽  
Murine Model ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Urine Volume ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome ◽  
Human Phenotype ◽  
Micturition Frequency

The pathophysiology of chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is poorly understood. Inflammatory and autoimmune mechanisms may play a role. We developed a murine model of experimental autoimmune prostatitis (EAP) that mimics the human phenotype of CP/CPPS. Eight-week-old mice were immunized subcutaneously with prostate-specific peptides in an emulsion of complete Freund's adjuvant. Mice were euthanized 10 days after immunization, and lymph node cells were isolated and assessed for recall proliferation to each peptide. P25 99–118 was the most immunogenic peptide. T-cell and B-cell immunity and serum levels of C-reactive protein and nitrate/nitrite levels were evaluated over a 9-wk period. Morphometric studies of prostate, 24-h micturition frequencies, and urine volume per void were evaluated. Tactile referred hyperalgesia was measured using von Frey filaments to the pelvic region. The unpaired Student's t-test was used to analyze differences between EAP and control groups. Prostates from p25 99–118-immunized mice demonstrated elevated gene expression levels of TNF-α, IL-17A, IFN-γ, and IL-1β, not observed in control mice. Compared with controls, p25 99–118-immunized mice had significantly higher micturition frequency and decreased urine output per void, and they demonstrated elevated pelvic pain response. p25 99–118 immunization of male SWXJ mice induced prostate-specific autoimmunity characterized by prostate-confined inflammation, increased micturition frequency, and pelvic pain. This autoimmune prostatitis model provides a useful tool for exploring the pathophysiology and new treatments.

To question of prophylaxis of the complicated motion of pregnancy at insufficiency and deficit of D-vitamin

2016 ◽  
pp. 19-27
Author(s):  
V. Pyrohova ◽  
◽  
S. Shurpyak ◽  
N.I. Zhemela ◽  
L. Holota ◽  
...  
Keyword(s):  
Key Words ◽  
Pelvic Pain ◽  
Chronic Pelvic Pain ◽  
Inflammatory Diseases ◽  
Symptomatic Therapy ◽  
Differentiation Process ◽  
National Medical ◽  
Medical University

Lviv national medical University named Danylo Galitsky In the article there are the lighted up questions of terminology, classification, diagnostics and treatment of syndrome of chronic pelvic pain. The features of diagnostics and differentiation process are considered in relation to the syndrome of chronic pelvic pain in gynecological practice. An accent is done, that treatment of chronic pelvic pain is fully determined a verification of basic disease, if it be impossible to find out primary cause (idiopathic genesis of chronic pelvic pain), symptomatic therapy is conducted. Key words: chronic pelvic pain, pelvic venocongestion, genital endometriosis, dysmenorrhea, pelvic inflammatory diseases.

scholarly journals MP53-20 REGULATION OF CHRONIC PELVIC PAIN VIA THE TRPV1 CHANNEL IN A MURINE MODEL OF CHRONIC PROSTATITIS

2019 ◽  
Vol 201 (Supplement 4) ◽  
Author(s):  
Kenny Roman* ◽  
Christel Hall ◽  
Anthony Schaeffer ◽  
Praveen Thumbikat
Keyword(s):  
Pelvic Pain ◽  
Murine Model ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Trpv1 Channel

scholarly journals Dose-dependent protective effect of nicotine in a murine model of viral myocarditis induced by coxsackievirus B3

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ge Li-Sha ◽  
Zhao Jing-Lin ◽  
Chen Guang-Yi ◽  
Liu Li ◽  
Zhou De-Pu ◽  
...  
Keyword(s):  
Murine Model ◽  
Group Treatment ◽  
Inflammatory Diseases ◽  
Viral Myocarditis ◽  
Coxsackievirus B3 ◽  
Left Ventricular ◽  
Untreated Group ◽  
Myocardial Inflammation ◽  
High Dose ◽  
Dose Dependent

Abstract The alpha 7 nicotinic acetylcholine receptor (alpha7 nAChR) was recently described as an anti-inflammatory target in various inflammatory diseases. The aim of this study was to investigate the dose-related effects of nicotine, an alpha7 nAChR agonist, in murine model of viral myocarditis. BALB/C mice were infected by an intraperitoneally injection with coxsackievirus B3. Nicotine was administered at doses of 0.1, 0.2 or 0.4 mg/kg three times per day for 7 or 14 consecutive days. The effects of nicotine on survival, myocardial histopathological changes, cardiac function and cytokine levels were studied. The survival rate on day 14 increased in a dose-dependent fashion and was markedly higher in the 0.2 and 0.4 mg/kg nicotine groups than in the infected untreated group. Treatment with high-dose nicotine reduced the myocardial inflammation and improved the impaired left ventricular function in infected mice. The mRNA expressions and protein levels of TNF-α, IL-1β, IL-6 and IL-17A were significantly downregulated in dose-dependent manners in the nicotine treatment groups compared to the infected untreated group. Nicotine dose-dependently reduced the severity of viral myocarditis through inhibiting the production of proinflammatory cytokines. The findings suggest that alpha7 nAChR agonists may be a promising new strategy for patients with viral myocarditis.

scholarly journals mMCP7, a Mouse Ortholog of δ Tryptase, Mediates Pelvic Tactile Allodynia in a Model of Chronic Pelvic Pain

2022 ◽  
Vol 2 ◽  
Author(s):  
Goutham Pattabiraman ◽  
Zhiqiang Liu ◽  
Madhumita Paul ◽  
Anthony J. Schaeffer ◽  
Praveen Thumbikat
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Pelvic Pain ◽  
Murine Model ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Nerve Fibers ◽  
Unknown Etiology ◽  
Pelvic Pain Syndrome ◽  
Tactile Allodynia

Chronic prostatitis/Chronic pelvic pain syndrome (CP/CPPS) is a condition that affects a large number of men and has unknown etiology. We have previously demonstrated the presence of elevated levels of mast cell tryptase in expressed prostatic secretions (EPS) of CP/CPPS patients. In a murine model of CP/CPPS, we showed tryptase and its cognate receptor PAR2 as critical to the development of pelvic pain and lower urinary tract symptoms. Here, we extend these observations to demonstrate that an isoform of tryptase called delta (δ)-tryptase, is elevated in the EPS of patients with CP/CPPS and is correlated with pelvic pain symptoms. Using an Escherichia coli (CP1) -induced murine model of CP/CPPS, we demonstrated a differential response in C57BL/6J and NOD/ShiLtJ mice, with C57BL6/J mice being resistant to an increase in pelvic tactile allodynia, despite having equivalent levels of activated mast cells in the prostate. Activated tryptase+ve mast cells were observed to be in closer apposition to PGP9.5+ve nerve fibers in the prostate stroma of NOD/ShiLtJ in comparison to C57BL/6J mice. The mouse ortholog of δ-tryptase, mouse mast cell protease 7 (mMCP7) has been reported to be unexpressed in C57BL/6J mice. We confirmed the absence of mMCP7 in the prostates of C57BL/6J and its presence in NOD/ShiLtJ mice. To evaluate a role for mMCP7 in the differential allodynia responses, we performed direct intra-urethral instillations of mMCP7 and the beta (β)-tryptase isoform ortholog, mMCP6 in the CP1-infection model. mMCP7, but not mMCP6 was able to induce an acute pelvic allodynia response in C57BL/6J mice. In-vitro studies with mMCP7 on cultured mast cells as well as dissociated primary neurons demonstrated the ability to induce differential activation of pain and inflammation associated molecules compared to mMCP6. We conclude that mMCP7, and possibility its human ortholog δ-tryptase, may play an important role in mediating the development of pelvic tactile allodynia in the mouse model of pelvic pain and in patients with CP/CPPS.

The capabilities of ultrasound study in the diagnosis of inflammatory diseases in women with chronic pelvic pain

2017 ◽  
pp. 57-60
Author(s):  
S.T. Ibadova ◽  
Keyword(s):  
Pelvic Pain ◽  
Chronic Pelvic Pain ◽  
Inflammatory Diseases ◽  
Pelvic Cavity ◽  
Heterogeneous Structure ◽  
Peritoneal Adhesions ◽  
Pelvic Organs ◽  
Ultrasound Study ◽  
Pain Patients ◽  
Acoustic Phenomenon

The objective: аn ultrasound study and analysis of the main echographic signs of inflammatory diseases of the uterus and adnexa in women with chronic pelvic pain. Patients and methods. Echographic examination of organs of small pelvis in 3D-mode was performed in 41 women, of which 25 (61.0%) patients with suspected inflammatory process of uterine appendages (oophoritis, salpingitis, tubo-ovarian education), 6 (14.6%) – inflammation of the uterus (endometritis) and 10 (24.4%) – inflammation of the cervix. Results. Found that in inflammatory diseases of the uterine appendages, in addition to the specific sonographic features, there is a common ultrasound markers of this process in the pelvis. These include pain in the study, the presence of fluid in the pelvic cavity, small, often moving hyperechoic inclusions with acoustic phenomenon, as well as peritoneal adhesions detected on the background fluid. Conclusions. For patients with endometritis was characterized by a heterogeneous structure of the endometrium, which is the presence of multiple mixed anechoic and hypoechoic inclusions, with a clear outline and the lack of it. For patients with endocervicitis was characterized by a heterogeneous structure of the mucosa, which was in the plural mixed anechoic and hypoechoic inclusions, having a clear contour. Key words: inflammatory diseases of the pelvic organs, pelvic pain, ultrasound.

95: Incidence and Significance of Prostatic Stones in Men with Chronic Prostatitis/Chronic Pelvic Pain Syndrome

2007 ◽  
Vol 177 (4S) ◽  
pp. 33-34
Author(s):  
Daniel A. Shoskes ◽  
Chun-Te Lee ◽  
Donel Murphy ◽  
John C. Kefer ◽  
Hadley M. Wood
Keyword(s):  
Pelvic Pain ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome

87: Biopsychosocial Factors in Quality of Life in men with Chronic Prostatitis/Chronic Pelvic Pain Syndrome

2007 ◽  
Vol 177 (4S) ◽  
pp. 31-31
Author(s):  
J. Curtis Nickel ◽  
Dean Tripp ◽  
Shannon Chuai ◽  
Mark S. Litwin ◽  
Mary McNaughton-Collins
Keyword(s):  
Quality Of Life ◽  
Pelvic Pain ◽  
Chronic Prostatitis ◽  
Chronic Pelvic Pain ◽  
Chronic Pelvic Pain Syndrome ◽  
Pain Syndrome ◽  
Pelvic Pain Syndrome
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