scholarly journals Erchen Decoction Prevents High-Fat Diet Induced Metabolic Disorders in C57BL/6 Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Bi-Zhen Gao ◽  
Ji-Cheng Chen ◽  
Ling-Hong Liao ◽  
Jia-Qi Xu ◽  
Xiao-Feng Lin ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Cell Function ◽  
Blood Lipid ◽  
Abdominal Circumference ◽  
High Fat ◽  
Medicine Prescription ◽  
Chinese Medicine Prescription ◽  
Treatment Of Obesity ◽  
Islet Cell Function

Erchen decoction (ECD) is a traditional Chinese medicine prescription, which is used in the treatment of obesity, hyperlipidemia, fatty liver, diabetes, hypertension, and other diseases caused by retention of phlegm dampness. In this study we investigated the potential mechanism of ECD, using metabolism-disabled mice induced by high-fat diet. Body weight and abdominal circumference were detected. OGTT was measured by means of collecting blood samples from the tail vein. Blood lipid levels and insulin were measured using biochemical assay kit. Real-time PCR was used to measure the CDKAL1 gene expression and western blot was used to measure the protein expression. Through the research, it was found that ECD showed markedly lower body weight and abdominal circumference than those in the HFD group. Consistently, we observed that ECD significantly improved glucose tolerance, promoted the secretion of insulin and decreased the level of TG, TC level. Meanwhile, we observed significantly increased CDKAL1 mRNA and protein level in the ECD group. Therefore, we speculate that the potential molecular mechanism of ECD is to promote the CDKAL1 expression, ameliorate islet cell function, and raise insulin levels to regulate the metabolic disorder.

scholarly journals Erchen Decoction Mitigates Lipid Metabolism Disorder by the Regulation of PPARγ and LPL Gene in a High-Fat Diet C57BL/6 Mice Model

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Mengting Zhang ◽  
Yanfei Shao ◽  
Bizhen Gao ◽  
Jicheng Chen ◽  
Ping Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Body Weight ◽  
Lipid Metabolism ◽  
Visceral Fat ◽  
Protein Level ◽  
High Fat Diet ◽  
Blood Lipid ◽  
Abdominal Circumference ◽  
High Fat ◽  
Gene And Protein Expression

Erchen decoction (ECD) is a common treatment prescribed in traditional Chinese medicine (TCM) clinics, which has remarkable efficacy in the treatment of obesity, fatty liver, hyperlipidemia, diabetes, and other diseases caused by phlegm. In this study, we investigated the effect that ECD had on the lipid metabolism induced by high-fat diet in C57BL/6 mice. Body weight, body length, and abdominal circumference were detected. Blood lipid content was measured via biochemical assay kit. The gene and protein expression of PPARγ and LPL in visceral fat and skeletal muscle of mice was measured by real-time PCR and western blot. The research discovered that the phlegm-resolving effect that ECD had on high-fat diet mice was mainly manifested as reduced body weight, Lee’s index, abdominal circumference, and level of TG and TC. Meanwhile, we observed significantly increased PPARγ mRNA and protein level in visceral fat and PPARγ and LPL protein level in skeletal muscle in the ECD group. Contrarily, a decrease in PPARγ mRNA level in skeletal muscle in the ECD group was observed. Therefore, we speculate that ECD regulates the lipid metabolic disorder by decreasing the blood lipid level. Moreover, the potential molecular mechanism of ECD is to promote the expression of PPARγ in visceral fat and skeletal muscle and the expression of LPL in skeletal muscle.

scholarly journals Effect of Citrus Limon Essential Oil on Lipid Profile and Obesity in Wistar Rats

2021 ◽  
pp. 1-5
Author(s):  
Souhaila Benchaoui ◽  
Meriem Gueracheha ◽  
Asma Boutebsou
Keyword(s):  
Body Weight ◽  
Essential Oil ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Intraperitoneal Administration ◽  
Female Rats ◽  
Abdominal Circumference ◽  
Citrus Limon ◽  
Standard Diet ◽  
High Fat

Obesity causes many pathologies; no therapeutic axis has provided an effective solution to this problem. The use of herbal medicine with healthy food and physical activity is recommended, and essential oils are the best known of these natural substances. The objective of this study is to evaluate the effect of Citrus limon essential oil on body weight and lipid profile. The plant material is the lemon; the extraction method is hydrodistillation. The experiment lasted 20 days and involved 15 female rats (2 to 3 months old) weighing between 160g and 230g. The rats were divided into 3 groups: the first receiving a standard diet, the second receiving a high-fat diet, and the third receiving a high-fat diet and treated with essential oil. The extraction yield is 1.69%. The physicochemical characteristics comply with AFNOR. This study shows that a high-lipid diet induces obesity characterized by hyperlipidemia. Intraperitoneal administration of the essential oil caused a decrease in body weight, abdominal circumference, plasma total cholesterol, and triglycerides and an increase in HDL-cholesterol. Considering this study, we found that lemon essential oil has beneficial effects on metabolic alterations. Its inclusion in the diet may help improve the metabolic profile and reduce the incidence of obesity and its long-term complications.

Hepatic PTP1B Expression Involvement in the Effects of Chinese Medicine Formula Xiao-Gao-Jiang-Zhuo Using an Obese Rat Model

2011 ◽  
Vol 39 (02) ◽  
pp. 301-313 ◽  
Author(s):  
Min Li ◽  
Bai Chang ◽  
Zhong Zhen ◽  
Pei-Jie Qin ◽  
Wen-Ke Liu ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Serum Insulin ◽  
Blood Lipid ◽  
Abdominal Fat ◽  
The Body ◽  
High Dose ◽  
Model Group ◽  
High Fat ◽  
Obese Rats

In this study, we investigated the effects of a Chinese herbal medicine formula Xiao-Gao-Jiang-Zhuo (XGJZ) in obese rats induced by a high-fat diet. Ten male rats in the normal group were fed with a standard diet. Another 50 male obese rats were induced by a 12-week high-fat diet feeding, and were randomly divided into five groups (n = 10 per group): the model group, the high-dose XGJZ group, the middle-dose XGJZ group, the low-dose XGJZ group, and the sibutramine group. After 14 weeks of treatment, body weight, abdominal fat, blood lipid and serum insulin level were measured, and the protein and gene expression of PTP1B in liver tissue was tested. Our data showed that the body weight of the high-dose and middle-dose groups and the sibutramine group had significant differences in comparison with the model group (p < 0.05), and all three dose groups had significantly reduced abdominal fat (p < 0.05). The triglyceride level of the three dose groups and the sibutramine group, and the total cholesterol level of the middle-dose group were all significantly reduced (p < 0.05). The serum insulin of the high-dose and middle-dose groups also decreased significantly (p < 0.05). The expression of hepatic PTP1B mRNA of the three dose groups decreased significantly in comparison with the model group (p < 0.05 or 0.01). The expression of hepatic PTP1B protein of the high-dose and middle-dose groups decreased significantly (p < 0.05). Our data suggested that XGJZ can modulate the body weight, abdominal fat and blood lipid in the obese rats, and this modulation might improve insulin resistance by inhibiting the PTP1B signal pathway.

scholarly journals Murinometric measurements and retroperitoneal adipose tissue in young rats exposed to the high-fat diet: Is there correlation?

2020 ◽  
Author(s):  
A. P. A Macêdo ◽  
G. S. Cordeiro ◽  
L. S. Santos ◽  
D. A. E. Santo ◽  
G. S. Perez ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Body Weight ◽  
Adipose Tissue ◽  
Body Mass ◽  
High Fat Diet ◽  
Longitudinal Axis ◽  
The Body ◽  
Abdominal Circumference ◽  
High Fat ◽  
Retroperitoneal Adipose Tissue

Abstract Aim This study aimed to verify the correlation between murine measurements and retroperitoneal adipose tissue in rats exposed to the high-fat diet. Material and methods: Wistar male adult rats, descendants of mothers who consumed a high-fat diet during pregnancy and lactation and fed the same diet after weaning were used. At 60 days of life, body weight, longitudinal axis and waist circumference (WC) were measured. The Body Mass Index (BMI) and the Lee Index were calculated for a posterior analysis of the correlation with the amount of retroperitoneal adipose tissue dissected on the same day. For analysis of the data, the Pearson correlation test was used, considering statistical significance for p <0.05. Results: Body weight had a weak correlation (r= 0.31; p= 0.38) with retroperitoneal adipose tissue. While the longitudinal correlated moderately and negative (r= -0.40; p= 0.25). Abdominal circumference (r= 0.62; p= 0.05), body mass index (r= 0.61; p= 0.03) and Lee (r= 0.69; p= 0.03) correlated moderately and positively with adipose tissue. Conclusion: Among the measured murine measurements, weight and longitudinal axis were not good indicators to represent accumulation of retroperitoneal adipose tissue in rats. However, Lee's index seems to be the best murine marker to diagnose the accumulation of retroperitoneal fat. BMI, CA and Lee index were murine parameters with higher correlation.

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