scholarly journals A New Technique for Quantitative Determination of Dexamethasone in Pharmaceutical and Biological Samples Using Kinetic Spectrophotometric Method

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Ali Mohammad Akhoundi-Khalafi ◽  
Masoud Reza Shishehbore
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Biological Samples ◽  
Reaction System ◽  
Calibration Graph ◽  
Experimental Conditions ◽  
Orange G ◽  
A New Technique ◽  
Kinetic Spectrophotometric

Dexamethasone is a type of steroidal medications that is prescribed in many cases. In this study, a new reaction system using kinetic spectrophotometric method for quantitative determination of dexamethasone is proposed. The method is based on the catalytic effect of dexamethasone on the oxidation of Orange G by bromate in acidic media. The change in absorbance as a criterion of the oxidation reaction progress was followed spectrophotometrically. To obtain the maximum sensitivity, the effective reaction variables were optimized. Under optimized experimental conditions, calibration graph was linear over the range 0.2–54.0 mg L−1. The calculated detection limit (3sb/m) was 0.14 mg L−1for six replicate determinations of blank signal. The interfering effect of various species was also investigated. The present method was successfully applied for the determination of dexamethasone in pharmaceutical and biological samples satisfactorily.

scholarly journals Determination of Sodium Cromoglycate by a New Kinetic Spectrophotometric Method in Biological Samples

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Mohsen Keyvanfard ◽  
Khadijeh Alizad ◽  
Razieh Shakeri
Keyword(s):  
Standard Deviation ◽  
Sodium Cromoglycate ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Micellar Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric

A new kinetic spectrophotometric method is described for the determination of ultratrace amounts of sodium cromoglycate (SCG). The method based on catalytic action of SCG on the oxidation of amaranth with periodate in acidic and micellar medium. The reaction was monitored spectrophotometrically by measuring the decrease in absorbance of the amaranth at 518 nm, for the first 4 min from initiation of the reaction. Calibration curve was linear in the range of 4.0−36.0 ng mL−1SCG. The limit of detection is 2.7 ng mL−1SCG. The relative standard deviation (RSD) for ten replicate analyses of 12, 20, and 28 ng mL−1SCG was 0.40%, 0.32%, and 0.53%, respectively. The proposed method was used for the determination of SCG in biological samples.

scholarly journals Kinetic- spectrophotometric Method for the Determination of Naringenin in Pure and Supplements Formulations

2019 ◽  
Vol 16 (3) ◽  
pp. 0595
Author(s):  
ALmashhadani Et al.
Keyword(s):  
Prussian Blue ◽  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Limit Of Detection ◽  
Fixed Time ◽  
Calibration Graph ◽  
Maximum Absorbance ◽  
Kinetic Spectrophotometric

          Simple, cheap, sensitive, and accurate kinetic- spectrophotometric method has been developed for the determination of naringenin in pure and supplements formulations. The method is based on the formation of Prussian blue. The product dye exhibits a maximum absorbance at 707 nm. The calibration graph of naringenin was linear over the range 0.3 to 10 µg ml-1 for the fixed time method (at 15 min) with a correlation coefficient (r) and percentage linearity (r2%) were of 0.9995 and 99.90 %, respectively, while the limit of detection LOD was 0.041 µg ml-1. The method was successfully applied for the determination of naringenin in supplements with satisfactory results.

scholarly journals Analytical application of the reaction system disulphonated hydroquinone-hydrogen peroxide for the kinetic spectrophotometric determination of iron traces in acidic media

2019 ◽  
Vol 73 (6) ◽  
pp. 387-396
Author(s):  
Snezana Tosic ◽  
Snezana Mitic ◽  
Aleksandra Pavlovic ◽  
Emilija Pecev-Marinkovic ◽  
Danijela Kostic ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Limit Of Detection ◽  
Reaction System ◽  
Absorption Spectrometry ◽  
Acidic Media ◽  
Experimental Conditions ◽  
Method Effects ◽  
Kinetic Spectrophotometric ◽  
White Radish

A simple, rapid, sensitive and selective kinetic spectrophotometric method for determination of Fe(III) traces was elaborated in this paper. It is based on the catalytic effect of Fe(III) ions on oxidation of potassium salt of disulphonated hydroquinone (K2S2Hy) by hydrogen peroxide in acidic media, at a constant ionic strength. At the working temperature of 20?C and the wavelength of 450.0 nm, optimal conditions for determination of iron were found so that iron (III) can be determined by the proposed method in the concentration range of 1.87 to 18.7 ng cm-3. Corresponding RSD values were determined to be in the range 4.22 to 10.33 %. The limit of detection (LOD) calculated in two ways was found to be 1.07 ng cm-3 i.e. 1.11 ng cm-3 Fe(III). In order to assess the selectivity of the method effects of different ions on the reaction rate were also determined. It was found that presence of oxalates and citrates in the w/w ratio to Fe(III) 1:1 under selected experimental conditions interferes with determination of iron. Then the method was applied for determination of Fe(III) traces in white radish juice. The results agreed well with those obtained by atomic absorption spectrometry.

A novel kinetic-spectrophotometric method for determination of nitrites in water

2009 ◽  
Vol 63 (4) ◽  
Author(s):  
Zenovia Moldovan
Keyword(s):  
Kinetic Method ◽  
Calibration Graph ◽  
Linear Calibration ◽  
Nitrite Concentration ◽  
Experimental Conditions ◽  
Sulfuric Acid Medium ◽  
Relative Standard ◽  
Kinetic Spectrophotometric ◽  
Drinking Water Samples

AbstractA simple, selective and sensitive kinetic method for the determination of nitrite in water was developed. The method is based on the catalytic effect of nitrite on the oxidation of methylene blue (MB) with bromate in a sulfuric acid medium. During the oxidation process, absorbance of the reaction mixture decreases with the increasing time, inversely proportional to the nitrite concentration. The reaction rate was monitored spectrophotometrically at λ = 666 nm within 30 s of mixing. Linear calibration graph was obtained in the range of 0.005–0.5 μg mL−1 with a relative standard deviation of 2.09 % for six measurements at 0.5 μg mL−1. The detection limit was found to be 0.0015 μg mL−1. The effect of different factors such as acidity, time, bromate concentration, MB concentration, ionic strength, and order of reactants additions is reported. Interference of the most common foreign ions was also investigated. The optimum experimental conditions were: 0.38 mol L−1 H2SO4, 5 × 10.4 mol L−1 KBrO3, 1.25 × 10.5 mol L−1 MB, 0.3 mol L−1 sodium nitrate, and 25°C. The proposed method was conveniently applied for the determination of nitrite in spiked drinking water samples.

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