scholarly journals Sliding Motility, Biofilm Formation, and Glycopeptidolipid Production inMycobacterium colombienseStrains

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Milena Maya-Hoyos ◽  
John Leguizamón ◽  
Leonardo Mariño-Ramírez ◽  
Carlos Y. Soto
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gene Cluster ◽  
Mycobacterium Avium ◽  
Biofilm Formation ◽  
Culture Conditions ◽  
Bioinformatics Analyses ◽  
Chromatographic Behaviour ◽  
Immunosuppressed Patients ◽  
Layer Chromatography

Mycobacterium colombienseis a novel member of theMycobacterium aviumcomplex, which produces respiratory and disseminated infections in immunosuppressed patients. Currently, the morphological and genetic bases underlying the phenotypic features ofM. colombiensestrains remain unknown. In the present study, we demonstrated thatM. colombiensestrains displaying smooth morphology show increased biofilm formation on hydrophobic surfaces and sliding on motility plates. Thin-layer chromatography experiments showed thatM. colombiensestrains displaying smooth colonies produce large amounts of glycolipids with a chromatographic behaviour similar to that of the glycopeptidolipids (GPLs) ofM. avium. Conversely, we observed a natural rough variant ofM. colombiense(57B strain) lacking pigmentation and exhibiting impaired sliding, biofilm formation, and GPL production. Bioinformatics analyses revealed a gene cluster that is likely involved in GPL biosynthesis inM. colombienseCECT 3035. RT-qPCR experiments showed that motile culture conditions activate the transcription of genes possibly involved in key enzymatic activities of GPL biosynthesis.

scholarly journals Subtyping ofMycobacterium aviumcomplex (MAC) isolates by thin-layer chromatography – distribution of subtypes from patients with AIDS compared with clinically non-significant isolates

1994 ◽  
Vol 112 (3) ◽  
pp. 543-549 ◽  
Author(s):  
W. Chew ◽  
T. C. Sorrell ◽  
G. L. Gilbert
Keyword(s):  
Confidence Interval ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mycobacterium Avium ◽  
Odds Ratio ◽  
Aids Patients ◽  
Significant Group ◽  
Layer Chromatography

SUMMARYThin-layer chromatography (TLC) was compared with seroagglutination for subtyping ofMycobacterium aviumcomplex (MAC) bacteria. Seventy-five significant MAC isolates from patients with AIDS were typed by both methods and 36 isolates, judged to be clinically non-significant, were examined by TLC only. Overall. 75% of isolates tested were typable by seroagglutination and 91% by TLC; the results correlated between the two except for minor discrepancies.Serovars 1, 8 and 21 and mixed serovars 1–21 and 1–8–21 were common among isolates from AIDS patients and together represented 83 % of isolates compared with only 36% in the non-significant group (odds ratio 8·4; 95% confidence interval 3·4–23·3). This difference remained significant after exclusion of serovar 41 (M. scrofulaceum), which was the commonest isolate (28%) in the non-significant group but was not isolated from patients with AIDS.TLC is useful to supplement seroagglutination for subtyping of MAC. Further study is required to determine whether apparent differences between isolates from patients with AIDS and from other sources reflect differences in virulence or in environmental prevalence of MAC subtypes.

Identification of atypical mycobacteria by thin-layer chromatography of their surface antigens

1978 ◽  
Vol 8 (4) ◽  
pp. 374-379
Author(s):  
P J Brennan ◽  
M Souhrada ◽  
B Ullom ◽  
J K McClatchy ◽  
M B Goren
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mycobacterium Avium ◽  
Surface Antigens ◽  
Atypical Mycobacteria ◽  
Layer Chromatography ◽  
Reference Strains

The knowledge that the surface (Schaefer) antigens of certain smooth-colony atypical mycobacteria are multiglycosylated C-mycosidic peptidoglycolipids was used to devise a sensitive thin-layer chromatographic (TLC) procedure for the identification of Mycobacterium avium/M. intracellulare/M. scrofulaceum serotypes. TLC maps of the type-specific peptidoglycolipids from 17 of the 31 serotypes are presented. The primary use of the technique is to corroborate results obtained by seroagglutination. Without the aid of seroagglutination, the TLC procedure almost invariably requires the availability of reference strains or the specific peptidoglycolipids derived therefrom.

Resolution of saturated and unsaturated 5β-cholanoic acids by gas–liquid and thin-layer chromatography

1979 ◽  
Vol 57 (6) ◽  
pp. 639-644 ◽  
Author(s):  
P. Child ◽  
A. Kuksis ◽  
J. J. Myher
Keyword(s):  
Thin Layer ◽  
Bile Acids ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Structural Information ◽  
Retention Factor ◽  
Chromatographic Behaviour ◽  
Spectrometric Data ◽  
Liquid Chromatographic ◽  
Layer Chromatography

The thin-layer and gas–liquid chromatographic properties of the methyl ester acetates were determined for a series of 20 monounsaturated 5β-cholanoic acids representing the simple chemical and enzymic dehydration products of the common bile acids. The unsaturated acids were generally indistinguishable from their saturated analogues by thin-layer chromatography on plain silica gel, but resolution was achieved on silica gel impregnated with silver nitrate for compounds having sterically exposed double bonds. The gas–liquid chromatographic behaviour of the unsaturated bile acids on the OV-225, SE-30, and Poly-S-179 liquid phases was closely similar to that observed for the saturated bile acids. The 5β-cholenoic acids obeyed the general rules of chromatographic mobility based on the overall shape of the molecule and the number and configuration of functional groups, with a constant retention factor attributable to the oleflnic bond. The structural information provided by the chromatographic behaviour of the standard unsaturated bile acids allows a distinction to be made among most of the isomeric 5β-cholenoates. A complete identification of all isomeric olefins is possible when chromatographic and mass spectrometric data are combined.

scholarly journals Chromatographic Behaviour of Antibiotics on Thin Layers of Zeolite

2019 ◽  
pp. 1-8
Author(s):  
Adham Raeisi ◽  
Mostafa Ramezani ◽  
Hossein Ravazadeh ◽  
Mahdi A. Taher
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Speed ◽  
High Performance ◽  
Low Cost ◽  
Current Method ◽  
Thin Layers ◽  
Chromatographic Behaviour ◽  
First Time ◽  
Layer Chromatography

Introduction: Antibiotics analysis is performed by many methods such as spectrophotometry, fluorimetry, polarography, and high-performance liquid chromatography. This analysis doesn't require derivatization but requires expensive equipment and extensive preparation. When more than one antibiotic is present in a formulation, interactions may occur between the drugs that must be separated before measurement. Thin-layer chromatography is a useful technique for identifying antibiotics because of the low cost, high speed, and low servicing. Silica gel adsorbents have often been used as adsorbents in all thin-layer chromatography studies. In this study, zeolite was used as an adsorbent in thin- layer chromatography with high selectivity. Materials and Methods: The chromatographic behaviour of amoxicillin, ampicillin, cefazolin, cefixime, ceftriaxone, cefalexin, and penicillin was studied for the first time on a thin layer of zeolite with mobile, organic, and organic- organic phases. Discussion: The best separation of ceftriaxone from amoxicillin, ampicillin, cefazolin, cefixolin, cefalexin, and penicillin on a thin layer of zeolite using methanol as the mobile phase. The distance and rise time are 12 cm and 110 minutes, respectively. Conclusion: The results of the present study showed that using the current method, the selectivity of one antibiotic from other components as well as two-component andthree-component adsorption was obtained. Quantitative identification of antibiotics was also performed in multicomponent mixtures after selection of appropriate isolates.

Sign in / Sign up

Export Citation Format

Share Document