The effect of methylene chain length on the chromatographic behaviour of aminobonded silicas in high-performance thin layer chromatography

1982 ◽  
Vol 16 (1) ◽  
pp. 152-154 ◽  
Author(s):  
M. Okamoto ◽  
F. Yamada ◽  
T. Omori
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chain Length ◽  
High Performance ◽  
Chromatographic Behaviour ◽  
Methylene Chain ◽  
Layer Chromatography

scholarly journals Chromatographic Behaviour of Antibiotics on Thin Layers of Zeolite

2019 ◽  
pp. 1-8
Author(s):  
Adham Raeisi ◽  
Mostafa Ramezani ◽  
Hossein Ravazadeh ◽  
Mahdi A. Taher
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Speed ◽  
High Performance ◽  
Low Cost ◽  
Current Method ◽  
Thin Layers ◽  
Chromatographic Behaviour ◽  
First Time ◽  
Layer Chromatography

Introduction: Antibiotics analysis is performed by many methods such as spectrophotometry, fluorimetry, polarography, and high-performance liquid chromatography. This analysis doesn't require derivatization but requires expensive equipment and extensive preparation. When more than one antibiotic is present in a formulation, interactions may occur between the drugs that must be separated before measurement. Thin-layer chromatography is a useful technique for identifying antibiotics because of the low cost, high speed, and low servicing. Silica gel adsorbents have often been used as adsorbents in all thin-layer chromatography studies. In this study, zeolite was used as an adsorbent in thin- layer chromatography with high selectivity. Materials and Methods: The chromatographic behaviour of amoxicillin, ampicillin, cefazolin, cefixime, ceftriaxone, cefalexin, and penicillin was studied for the first time on a thin layer of zeolite with mobile, organic, and organic- organic phases. Discussion: The best separation of ceftriaxone from amoxicillin, ampicillin, cefazolin, cefixolin, cefalexin, and penicillin on a thin layer of zeolite using methanol as the mobile phase. The distance and rise time are 12 cm and 110 minutes, respectively. Conclusion: The results of the present study showed that using the current method, the selectivity of one antibiotic from other components as well as two-component andthree-component adsorption was obtained. Quantitative identification of antibiotics was also performed in multicomponent mixtures after selection of appropriate isolates.

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