scholarly journals Extract ofAllium tuberosum Rottler ex SprengPromoted the Hair Growth through Regulating the Expression of IGF-1

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Ki Moon Park ◽  
Dong Woo Kim ◽  
Seung Ho Lee
Keyword(s):  
Growth Factor ◽  
Hair Growth ◽  
Control Mouse ◽  
Ethanol Extract ◽  
Insoluble Fraction ◽  
Hair Follicles ◽  
Gel Chromatography ◽  
Skin Area ◽  
Dorsal Skin ◽  
Growth Promoting

Allium tuberosum Rottler ex Spreng(ATRES) has been used as a traditional medicine for the treatment of abdominal pain, diarrhea, and asthma. In this study, we investigated the hair growth promoting activities of ATRES on telogenic C57BL6/N mice. Hair growth was significantly increased in the dorsal skin of ethanol extract of ATRES treated mouse group compared with the control mouse group. To enrich the hair promoting activity, an ethanol-insoluble fraction was further extracted in sequence withn-hexane, dichloromethane, ethyl acetate,n-butanol, and distilled water. Interestingly, we found that extraction withn-butanol is most efficient in producing the hair promoting activity. In addition, the soluble fraction of then-butanol extract was further separated by silica gel chromatography and thin layer chromatography (TLC) resulting in isolating four single fractions which have hair growth regeneration potential. Furthermore, administration of ATRES extracts to dorsal skin area increased the number of hair follicles compared with control mouse group. Interestingly, administration of ATRES extract stimulated the expression of insulin-like growth factor-1 (IGF-1) but not of keratin growth factor (KGF) or vascular endothelial growth factor (VEGF). Taken together, these results suggest that ATRES possesses strong hair growth promoting potential which controls the expression of IGF-1.

scholarly journals Fibroblast Growth Factors Stimulate Hair Growth throughβ-Catenin and Shh Expression in C57BL/6 Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Wei-hong Lin ◽  
Li-Jun Xiang ◽  
Hong-Xue Shi ◽  
Jian Zhang ◽  
Li-ping Jiang ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Hair Growth ◽  
Hair Follicles ◽  
Control Group ◽  
Fibroblast Growth ◽  
Acidic Fibroblast Growth Factor ◽  
Anagen Phase ◽  
Growth Promoting

Growth factors are involved in the regulation of hair morphogenesis and cycle hair growth. The present study sought to investigate the hair growth promoting activities of three approved growth factor drugs, fibroblast growth factor 10 (FGF-10), acidic fibroblast growth factor (FGF-1), and basic fibroblast growth factor (FGF-2), and the mechanism of action. We observed that FGFs promoted hair growth by inducing the anagen phase in telogenic C57BL/6 mice. Specifically, the histomorphometric analysis data indicates that topical application of FGFs induced an earlier anagen phase and prolonged the mature anagen phase, in contrast to the control group. Moreover, the immunohistochemical analysis reveals earlier induction ofβ-catenin and Sonic hedgehog (Shh) in hair follicles of the FGFs-treated group. These results suggest that FGFs promote hair growth by inducing the anagen phase in resting hair follicles and might be a potential hair growth-promoting agent.

scholarly journals Evaluation of hair growth promoting activity of petroleum ether extract of Abrus precatorius Linn. on Wistar Albino rats

2019 ◽  
Vol 9 (2) ◽  
pp. 330-335
Author(s):  
Namrata Omhare ◽  
Pratibha Sahu ◽  
Shailee Gautam ◽  
Nidhi Jain
Keyword(s):  
Petroleum Ether ◽  
Hair Growth ◽  
Petroleum Ether Extract ◽  
Hair Follicles ◽  
Ether Extract ◽  
Standard Drug ◽  
Abrus Precatorius ◽  
Growth Initiation ◽  
Growth Promoting ◽  
Bald Patch

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords: Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidil

scholarly journals Effects of Decursin and Angelica gigas Nakai Root Extract on Hair Growth in Mouse Dorsal Skin via Regulating Inflammatory Cytokines

Molecules ◽  
2020 ◽  
Vol 25 (16) ◽  
pp. 3697
Author(s):  
Tae-Kyeong Lee ◽  
Bora Kim ◽  
Dae Won Kim ◽  
Ji Hyeon Ahn ◽  
Hyejin Sim ◽  
...  
Keyword(s):  
Inflammatory Cytokines ◽  
Hair Growth ◽  
Hair Follicles ◽  
Control Group ◽  
Root Extract ◽  
Dorsal Skin ◽  
Angelica Gigas ◽  
Angelica Gigas Nakai ◽  
Tnf Α ◽  
Anti Inflammatory

This current study investigates the facilitative effects and mechanisms of decursin, a major component of Angelica gigas Nakai (AGN), and AGN root extract on hair growth in mice. We perform high-performance liquid chromatography on AGN extract to show it contains 7.3% decursin. Hairs in mouse dorsal skin are shaved distilled in water, 0.15% decursin, and 2% AGN root extract (0.15% decursin in the diluted extract) and topically applied twice a day for 17 days. Hematoxylin and eosin staining are done to examine the morphological changes in the hair follicles. To compare the effects of decursin and AGN extract on inflammatory cytokines in the dorsal skin, Western blot analysis and immunohistochemistry for tumor necrosis factor α (TNF-α) and interleukin (IL)-1β as pro-inflammatory cytokines, and IL-4 and IL-13 as anti-inflammatory cytokines are conducted. The results show that the application of decursin and AGN extract confer effects on hair growth. Hair growth is significantly facilitated from seven days after the treatments compared to that in the control group, and completely grown hair was found 17 days after the treatments. The protein levels and immunoreactivity of TNF-α and IL-1β in this case are significantly decreased, whereas the IL-4 and IL-13 levels and immunoreactivity are significantly increased compared to those in the control group. Additionally, high-mobility group box 1, an inflammatory mediator, is elevated by the topical application of decursin and AGN extract. Taken together, the treatment of mouse dorsal skin with AGE root extract containing decursin promotes hair growth by regulating pro- and/or anti-inflammatory cytokines. We, therefore, suggest that AGN root extract as well as decursin can be utilized as materials for developing hair growth-facilitating treatments.

scholarly journals Shikimic acid, a mannose bioisostere, promotes hair growth with the induction of anagen hair cycle

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mira Choi ◽  
Soon-Jin Choi ◽  
Sunhyae Jang ◽  
Hye-In Choi ◽  
Bo-Mi Kang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Organ Culture ◽  
Human Hair ◽  
Hair Growth ◽  
Shikimic Acid ◽  
Ex Vivo ◽  
Hair Follicles ◽  
Anagen Hair

AbstractShikimic acid (SA) has recently been found to be a major component of plant stem cells. The exact effects of SA on human hair follicles (HFs) is unknown. The purpose of this study was to examine the effects of SA on hair growth. We investigated the effect of SA on an in vivo C57BL/6 mouse model. We examined the expression of mannose receptor (MR), which is a known receptor of SA, in human HFs and the effect of SA on human dermal papilla cells (hDPCs), outer root sheath cells (hORSCs), and on ex vivo human hair organ culture. SA significantly prolonged anagen hair growth in the in vivo mouse model. We confirmed expression of the MR in human HFs, and that SA increased the proliferation of hDPCs and hORSCs. It was found that SA enhanced hair shaft elongation in an ex vivo human hair organ culture. SA treatment of hDPCs led to increased c-myc, hepatocyte growth factor, keratinocyte growth factor and vascular endothelial growth factor levels and upregulation of p38 MAPK and cAMP response element-binding protein levels. Our results show that SA promotes hair growth and may serve as a new therapeutic agent in the treatment of alopecia.

Hair Growth-Promoting Activity of Citron Seed Oil: Induction of Premature Telogen-To-Anagen Transition of Hair Follicle in C57BL/6 Mouse Models

2021 ◽  
Vol 20 (1) ◽  
pp. 28-36
Author(s):  
Van-Long Truong ◽  
Woo-Sik Jeong
Keyword(s):  
Growth Factor ◽  
Hair Follicle ◽  
Hair Growth ◽  
Seed Oil ◽  
Growth Promotion ◽  
Mouse Skin ◽  
Raw Material ◽  
Skin Thickness ◽  
Citrus Junos ◽  
Growth Promoting

Citron (Citrus junos Sieb. Ex Tanaka), a citrus fruit native to Korea, Japan, and China, has been commonly used as a raw material for beverages and herbal medicines. Citron seeds, the by-product of processing industry, contain many bioactive compounds that exhibit several biological properties, such as antioxidative, anti-inflammatory, and antiobesity. However, potential of citron seed oil for hair growth promotion remains unexplored. In the present study, we investigated the hair growth-promoting effect of citron seed oil and its underlying mechanism(s) in a C57BL/6 mouse model. The results show that citron seed oil effectively promotes hair regrowth by early induction of telogen-to-anagen transition and by increasing hair follicle density and hair bulb diameter as well as skin thickness. Citron seed oil treatment increased β-catenin, lymphoid enhancer binding factor 1, sonic hedgehog, cyclin D1 and cyclin E expressions, and alkaline phosphatase activity, compared to the vehicle-treated control mice. Topical administration of citron seed oil upregulated expression of antiapoptotic Bcl-2 protein but did not alter protein level for proapoptotic Bax in mouse skin tissues. Furthermore, insulin-like growth factor 1 and vascular endothelial growth factor were also induced by citron seed oil treatment. These findings suggest the potential of citron seed oil in the prevention and/or treatment of hair loss by inducing hair cycling and regeneration.

scholarly journals Macrophage-Derived Extracellular Vesicle Promotes Hair Growth

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 856 ◽  
Author(s):  
Ramya Lakshmi Rajendran ◽  
Prakash Gangadaran ◽  
Chang Hoon Seo ◽  
Mi Hee Kwack ◽  
Ji Min Oh ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hair Loss ◽  
Hair Growth ◽  
Keratinocyte Growth Factor ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Wnt Proteins ◽  
Short Period ◽  
Phosphorylated Akt

Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (Axin2 and Lef1). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.

scholarly journals Hair Growth is Promoted in Mouse Dorsal Skin by a Mixture of Platycladus orientalis (L.) Franco Leaf Extract and Alpha-Terpineol by Increasing Growth Factors and wnt3/β-Catenin

2020 ◽  
Vol 15 (8) ◽  
pp. 1934578X2095143
Author(s):  
Ji Hyeon Ahn ◽  
Young Eun Park ◽  
Bora Kim ◽  
Cheol Woo Park ◽  
Tae Heung Sim ◽  
...  
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Hair Growth ◽  
Leaf Extract ◽  
Control Group ◽  
Growth Promoter ◽  
Dorsal Skin ◽  
Ki 67 ◽  
Total N ◽  
Platycladus Orientalis

In this study, we investigated the effect of a mixture of Platycladus orientalis (L.) Franco leaf extract and α-terpineol, a natural monoterpene alcohol (PEaT), on hair growth and its mechanisms. C57/BL6 mice (total n = 14) in the telogen phase of hair growth were used. Either distilled water as vehicle or PEaT was topically applied to the dorsal skin for 17 days. Chronological hair growth change was examined by hair growth-promoting scores. In addition, to find out mechanisms of PEaT on hair growth, insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), Ki-67, wnt3, and β-catenin expressions were investigated by using immunohistochemistry. We found that PEaT remarkably promoted hair growth by inducing early anagen transition compared with the control group. In addition, treatment with PEaT significantly increased numbers of Ki-67-positive cells and expressions of IGF-1, VEGF, wnt3, and β-catenin in the outer root sheath. These results indicate that PEaT used in this study might be a good hair growth promoter, showing that PEaT treatment increased growth factors and cell proliferation through upregulation of wnt3 and β-catenin expressions.

scholarly journals Kojyl Cinnamate Ester Derivatives Increase Adiponectin Expression and Stimulate Adiponectin-Induced Hair Growth Factors in Human Dermal Papilla Cells

2019 ◽  
Vol 20 (8) ◽  
pp. 1859 ◽  
Author(s):  
Phil June Park ◽  
Eun-Gyung Cho
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Mrna Expression ◽  
Hair Growth ◽  
Subcutaneous Fat ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Glucose Levels ◽  
Endothelial Growth Factor

Adiponectin (APN), released mainly from adipose tissue, is a well-known homeostatic factor for regulating glucose levels, lipid metabolism, and insulin sensitivity. A recent study showed that human hair follicles express APN receptors and the presence of APN-mediated hair growth signaling, thereby suggesting that APN is a potent hair growth-promoting adipokine. Previously, kojyl cinnamate ester derivatives (KCEDs) were synthesized in our institute as new anti-aging or adiponectin-/adipogenesis-inducing compounds. Here, we tested the activity of these derivatives to induce endogenous APN secretion. Among the derivatives, KCED-1 and KCED-2 showed improved activity in inducing APN mRNA expression, secretion of APN protein, and adipogenesis in human subcutaneous fat cells (hSCFs) when compared with the effects of Seletinoid G, a verified APN inducer. When human follicular dermal papilla cells were treated with the culture supernatant of KCED-1- or KCED-2-treated hSCFs, the mRNA expression of APN-induced hair growth factors such as insulin-like growth factor, hepatocyte growth factor, and vascular endothelial growth factor was upregulated compared with that in the control. Taken together, our study shows that among kojyl cinnamate ester derivatives, KCED-1, KCED-2, as well as Seletinoid G are effective inducers of endogenous APN production in subcutaneous fat tissues, which may in turn contribute to the promotion of hair growth in the human scalp.

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