scholarly journals Macrophage-Derived Extracellular Vesicle Promotes Hair Growth

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 856 ◽  
Author(s):  
Ramya Lakshmi Rajendran ◽  
Prakash Gangadaran ◽  
Chang Hoon Seo ◽  
Mi Hee Kwack ◽  
Ji Min Oh ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hair Loss ◽  
Hair Growth ◽  
Keratinocyte Growth Factor ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Wnt Proteins ◽  
Short Period ◽  
Phosphorylated Akt

Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (Axin2 and Lef1). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.

scholarly journals Formulation and Evaluation of Herbal Hair Gel Containing Fenugreek Seed Extract for Nourishment and Hair Growth

2019 ◽  
pp. 92-103
Author(s):  
Suryawanshi Nishant C ◽  
Vijayendra Swamy S. M. ◽  
Nagoba Shivappa N. ◽  
Wanje Vaijanti V.
Keyword(s):  
Hair Loss ◽  
Hair Growth ◽  
Estrogen Therapy ◽  
Hair Follicles ◽  
Hormone Deficiency ◽  
Gelling Agent ◽  
Nutritional Deficiencies ◽  
Topical Formulation ◽  
Fenugreek Seed

The present study now a days many people face the major problem related to hair i.e. hair loss. There are many causes of hair loss physiological conditions, emotional or physical stress, nutritional deficiencies, hormonal disorders one of the due to hormone deficiency of estrogen. External administration of the estrogen could changes the hormonal cycle and increase cancer risk some natural alternative estrogen therapy can be found in the various plants containing natural products those having weak estrogen activity like Phyto-estrogen. Herbal drug has less side effects and more effective as comparative to synthetic drug. Phytoestrogen are competing with the estrogen by the binding to the estrogen receptor and produce estrogen effect, Phytoestrogen in the fenugreek seed. Family – Fabaceae Ethanolic extract of (Trigonella foenum-graecum) fenugreek seed prepared for the topical formulation of herbal hair gel formulation by using Carbopol 934 gelling agent, glycerin, pvp, methyl paraben, PEG, Triethanolamine Fenugreek was evaluated for its potency on hair growth activity by in vivo method. In vivo, study 2.5mg of fenugreek extract is used. That is applied on the shaved skin of mice to determine the length of hair and the different cyclic phase of hair follicles like anagen and s phases were will be grow after some time periods. From the study topical use of gel formulation were apply for 30 days .There are use of fenugreek extract containing gel formulation over the shaved skin of mice that shows the significant result by increase the hair growth. The prepared gel was characterized for their physicochemical constants, preliminary phyto-chemical analysis, quantitative analysis, Spread-ability, pH, viscosity, and stability study.

scholarly journals Shikimic acid, a mannose bioisostere, promotes hair growth with the induction of anagen hair cycle

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mira Choi ◽  
Soon-Jin Choi ◽  
Sunhyae Jang ◽  
Hye-In Choi ◽  
Bo-Mi Kang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mouse Model ◽  
Organ Culture ◽  
Human Hair ◽  
Hair Growth ◽  
Shikimic Acid ◽  
Ex Vivo ◽  
Hair Follicles ◽  
Anagen Hair

AbstractShikimic acid (SA) has recently been found to be a major component of plant stem cells. The exact effects of SA on human hair follicles (HFs) is unknown. The purpose of this study was to examine the effects of SA on hair growth. We investigated the effect of SA on an in vivo C57BL/6 mouse model. We examined the expression of mannose receptor (MR), which is a known receptor of SA, in human HFs and the effect of SA on human dermal papilla cells (hDPCs), outer root sheath cells (hORSCs), and on ex vivo human hair organ culture. SA significantly prolonged anagen hair growth in the in vivo mouse model. We confirmed expression of the MR in human HFs, and that SA increased the proliferation of hDPCs and hORSCs. It was found that SA enhanced hair shaft elongation in an ex vivo human hair organ culture. SA treatment of hDPCs led to increased c-myc, hepatocyte growth factor, keratinocyte growth factor and vascular endothelial growth factor levels and upregulation of p38 MAPK and cAMP response element-binding protein levels. Our results show that SA promotes hair growth and may serve as a new therapeutic agent in the treatment of alopecia.

scholarly journals Stimulation of all epithelial elements during skin regeneration by keratinocyte growth factor.

1994 ◽  
Vol 179 (3) ◽  
pp. 831-840 ◽  
Author(s):  
G F Pierce ◽  
D Yanagihara ◽  
K Klopchin ◽  
D M Danilenko ◽  
E Hsu ◽  
...  
Keyword(s):  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Inflammatory Cells ◽  
Hair Follicles ◽  
Critical Determinant ◽  
Keratinocyte Proliferation ◽  
Potential Capacity ◽  
Proliferation And Differentiation ◽  
Normal Differentiation

Keratinocyte growth factor (KGF), a recently discovered 18.9 kD member of the fibroblast growth factor family has been shown to selectively induce keratinocyte proliferation and differentiation in tissue culture. To explore its potential stimulating keratinocyte growth and differentiation in vivo, we analyzed for the influence of KGF on epithelial derived elements within a wound created through the cartilage on the rabbit ear. KGF accelerated reepithelialization (p = 0.004) and increased the thickness of the epithelium (p = 0.0005) when 4-40 micrograms/cm2 recombinant KGF was added at the time of wounding. The regenerating epidermis showed normal differentiation as detected by cytokeratin immunostaining. Remarkably, however, KGF stimulated proliferation and differentiation of early progenitor cells within hair follicles and sebaceous glands in the wound bed and adjacent dermis. There was a transient but highly significant increase in specific labeling of cycling cells in both basal and suprabasal layers that extended into the spinous layer of the regenerating epidermis. As an indication of specificity, the inflammatory cells and fibroblasts within the wound were not influenced by KGF. The results indicate that KGF is unique in its ability to accelerate reepithelialization and dermal regeneration by targeting multiple epithelial elements within the skin. These results suggest that KGF may induce specific epithelial progenitor cell lineages within the skin to proliferate and differentiate, and thus may be a critical determinant of regeneration of skin. Furthermore, these findings illustrate the potential capacity of this system to analyze epithelial differentiation programs and disorders of epidermis, dermal glandular elements, and hair follicles.

scholarly journals Frontier in hair loss and trichoscopy: A review

2016 ◽  
Vol 1 (2) ◽  
Author(s):  
Ebtisam Elghblawi
Keyword(s):  
Hair Loss ◽  
Skin Lesions ◽  
Hair Shaft ◽  
Hair Follicles ◽  
Invasive Technique ◽  
Non Invasive ◽  
Exclamation Mark ◽  
Indispensable Tool ◽  
Hair Diseases

<p>Skin surfaces have always been examined using dermoscopy, a familiar tool which is useful to magnify and examine skin especially in cases of pigmented skin lesions. However, to examine the hair and scalp, a practical tool called trichoscopy has surfaced recently and has proven to be handy and functional in diagnosing most hair-related diseases. It is also referred to as dermoscopy of the hair and the scalp. It can aid in assessing active diseases in the scalp and hair, such as yellow dots, dystrophic hairs, cadaverized black dots, white dots, and exclamation mark hairs – all of which denote specific criteria for hair diseases. Trichoscopy is a very newly developed non-invasive technique for hair image analysis. It permits non-invasive visualization of hair shafts at higher intensification (about ×70 and ×100) and enables measurement of hair shaft width without the need for removing hair for diagnostic reasons. Moreover, it helps <em>in vivo</em> visualization of the epidermal portion of hair follicles and perifollicular epidermis (orifices). Consequently, it is valuable as it permits the inspection of structures that are otherwise not seen by the naked eye. Trichoscopy is the new frontier for the diagnosis of hair and scalp disease. Nowadays, a trichoscope is considered a must for dermatologists and it is a hot topic in the treatment of hair diseases. There is pooled evidence that the utilization of trichoscopy in the clinical setting for evaluating hair disorders can improve its diagnostic capability beyond simple clinical scrutiny. Trichoscopy can identify both hair shaft and hair opening abnormalities without the need for hair sampling, as well as distinguish between different scalp and hair diseases. Furthermore, it can give easy and quick evaluation of the hair with a follow-up to determine progress and prognosis of the disease with photos. It can also aid in some genetic hair shaft dystrophies such as trichorrhexis nodosa, trichorrhexis invaginata, monilethrix, pili annulati, and pili torti. The limitation of trichoscopy is that it needs prior knowledge to apply it effectively in order to mandate an efficient use by correctly interpreting the findings and their significance. In cases where there are unsettled discrepancies, a histopathological investigation is needed. The interest in trichoscopy has vastly increased and has become an indispensable tool in evaluating patients with hair loss. The aim of this review is to supplement existing knowledge on trichoscopy with recent readings of different scalp and hair conditions that are commonly encountered in clinical settings.</p>

scholarly journals Annurca Apple Polyphenols Protect Murine Hair Follicles from Taxane Induced Dystrophy and Hijacks Polyunsaturated Fatty Acid Metabolism toward β-Oxidation

Nutrients ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1808 ◽  
Author(s):  
Gennaro Riccio ◽  
Eduardo Sommella ◽  
Nadia Badolati ◽  
Emanuela Salviati ◽  
Sara Bottone ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Hair Loss ◽  
Hair Growth ◽  
Treatment Options ◽  
Pentose Phosphate ◽  
Hair Follicles ◽  
Common Side Effect ◽  
Safe Alternative ◽  
Polyphenolic Extract

Chemotherapy-induced alopecia (CIA) is a common side effect of conventional chemotherapy and represents a major problem in clinical oncology. Even months after the end of chemotherapy, many cancer patients complain of hair loss, a condition that is psychologically difficult to manage. CIA disturbs social and sexual interactions and causes anxiety and depression. Synthetic drugs protecting from CIA and endowed with hair growth stimulatory properties are prescribed with caution by oncologists. Hormones, growth factors, morphogens could unwontedly protect tumour cells or induce cancer cell proliferation and are thus considered incompatible with many chemotherapy regimens. Nutraceuticals, on the contrary, have been shown to be safe and effective treatment options for hair loss. We here show that polyphenols from Malus Pumila Miller cv Annurca are endowed with hair growth promoting activity and can be considered a safe alternative to avoid CIA. In vitro, Annurca Apple Polyphenolic Extract (AAE) protects murine Hair Follicles (HF) from taxanes induced dystrophy. Moreover, in virtue of its mechanism of action, AAE is herein proven to be compatible with chemotherapy regimens. AAE forces HFs to produce ATP using mitochondrial β-oxidation, reducing Pentose Phosphate Pathway (PPP) rate and nucleotides production. As consequence, DNA replication and mitosis are not stimulated, while a pool of free amino acids usually involved in catabolic reactions are spared for keratin production. Moreover, measuring the effect exerted on Poly Unsaturated Fatty Acid (PUFA) metabolism, we prove that AAE promotes hair-growth by increasing the intracellular levels of Prostaglandins F2α (PGF2α) and by hijacking PUFA catabolites toward β-oxidation.

scholarly journals A Novel Solid-Phase Site-Specific PEGylation Enhances the In Vitro and In Vivo Biostabilty of Recombinant Human Keratinocyte Growth Factor 1

PLoS ONE ◽  
2012 ◽  
Vol 7 (5) ◽  
pp. e36423 ◽  
Author(s):  
Zhifeng Huang ◽  
Guanghui Zhu ◽  
Chuanchuan Sun ◽  
Jingui Zhang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Growth Factor ◽  
Solid Phase ◽  
Keratinocyte Growth Factor ◽  
Site Specific ◽  
Human Keratinocyte
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