scholarly journals Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid Mesh

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Meng Deng ◽  
Yunpeng Gu ◽  
Zhenjun Liu ◽  
Yue Qi ◽  
Gui E. Ma ◽  
...  
Keyword(s):  
Polylactic Acid ◽  
3D Culture ◽  
Initial Step ◽  
Polyglycolic Acid ◽  
Endothelial Differentiation ◽  
Positive Ratio ◽  
Endothelial Phenotype ◽  
Human Adscs ◽  
Uptake Assay ◽  
Prevascularized Tissue

Adipose-derived stem cell (ADSC) is considered as a cell source potentially useful for angiogenesis in tissue engineering and regenerative medicine. This study investigated the growth and endothelial differentiation of human ADSCs on polyglycolic acid/polylactic acid (PGA/PLA) mesh compared to 2D plastic. Cell adhesion, viability, and distribution of hADSCs on PGA/PLA mesh were observed by CM-Dil labeling, live/dead staining, and SEM examination while endothelial differentiation was evaluated by flow cytometry, Ac-LDL/UEA-1 uptake assay, immunofluorescence stainings, and gene expression analysis of endothelial related markers. Results showed hADSCs gained a mature endothelial phenotype with a positive ratio of 21.4 ± 3.7% for CD31+/CD34− when induced in 3D mesh after 21 days, which was further verified by the expressions of a comprehensive range of endothelial related markers, whereas hADSCs in 2D induced and 2D/3D noninduced groups all failed to differentiate into endothelial cells. Moreover, compared to 2D groups, the expression forα-SMA was markedly suppressed in 3D cultured hADSCs. This study first demonstrated the endothelial differentiation of hADSCs on the PGA/PLA mesh and pointed out the synergistic effect of PGA/PLA 3D culture and growth factors on the acquisition of mature characteristic endothelial phenotype. We believed this study would be the initial step towards the generation of prevascularized tissue engineered constructs.

scholarly journals DEGRADASI POLIBLEND POLI(ASAMLAKTAT-KO-ASAMGLIKOLAT) DENGAN POLI(ε-KAPROLAKTON) SECARA IN VITRO

2015 ◽  
Vol 2 (2) ◽  
pp. 174
Author(s):  
Tetty Kemala ◽  
Achmad Sjahriza ◽  
Hendra Adijuwana ◽  
Mardiana Hardianti
Keyword(s):  
Lactic Acid ◽  
Polylactic Acid ◽  
Intrinsic Viscosity ◽  
Phosphate Buffer ◽  
Glycolic Acid ◽  
Incubation Temperature ◽  
Human Life ◽  
Polyglycolic Acid ◽  
Poly Lactic Acid

 ABSTRACT  Polymer has many applications in human life, one of them is in the field of health. Polymer synthetics like polylactic acid (PLA), polyglycolic acid (PGA), poly(ε-caprolactone) (PCL), and poly(lactic acid-co-glycolic acid) (PLGA) were kind of polyesters that is many used in field   of health. Many researchers have already made research about degradation of PLA, PGA, PCL, and PLGA. However, there is no one doing research about degradation of combination between this two polymers, one of them is polyblend of  PLGA and PCL. Polyblend were made of four compositions, that were PLGA(90:10):PCL 3:1, PLGA(75:25):PCL 3:1, PLGA(90:10):PCL 5:1, and PLGA(75:25):PCL 5:1. Degradation was carried out for eight weeks by using phosphate buffer pH 7.4 and incubation temperature of 37°C. Degradation of the polymer was observed by mass remained, and intrinsic viscosity. The result showed that composition PLGA(75:25):PCL 5:1 was the fastest in degradation compared to other compositions. It was showed by the decrease in mass until 89.06% and the change in intrinsic viscosity until 20.13%. Keywords: PLGA, PCL   

scholarly journals The Advantages of Bioresorbable INION� Implants in Traumatology Design, polymer composition and preliminary results

2019 ◽  
Vol 56 (1) ◽  
pp. 47-50
Author(s):  
Andrei Scripcaru ◽  
Norin Forna ◽  
Alexandru Bogdan Ciubara ◽  
Horea Rares Ciprian Benea ◽  
Vlad Veringa ◽  
...  
Keyword(s):  
Polylactic Acid ◽  
Stress Shielding ◽  
Polyglycolic Acid ◽  
Polymer Composition ◽  
Trimethylene Carbonate ◽  
Metallic Implants ◽  
Implant Complications ◽  
Low Profile ◽  
Resorbable Implants ◽  
Alpha Hydroxy Acids

Some disadvantages of traditional metallic implants used in orthopedics and traumatology prompted the development of bioresorbable polymer devices.The aim of this experimental study is to emphasize the characteristics of INION� resorbable implants (regarding design and polymers compositions), as well as to evaluate the results when using these innovative implants in two trauma cases. The polymers used in manufacturing INION� devices (Trimethylene Carbonate/TMC; L-Polylactic acid/LPLA; D,L Polylactic acid/DLPLA; Polyglycolic acid/PGA) degrade in alpha-hydroxy acids, gradually losing their hardness in 18-36 weeks with a complete bioresorption of 2-4 years. The clinical cases demonstrated the advantages of INION� plates (adapted shape, low profile, polyaxial screws, acceptable strength) or pins (allowing the aligmment and fixation of fracture, no migration). Among our patients, we found excellent results concerning the maintaining of primary reduced fracture, active range of motion, minimal pain with improving everyday comfort, no tissue or implant complications. Bioresorbable fracture fixation INION� devices are a viable alternative to traditional metallic implants, offering same significant advantages over them: the avoidance of long-term interference with gliding structures, keeping their strength long enough to support bone healing, no need to remove the implants, less pain, radiolucency, elimination of stress shielding and a lower risk of complications.

Adequacy of herniated disc tissue as a cell source for nucleus pulposus regeneration

2011 ◽  
Vol 14 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Aldemar A. Hegewald ◽  
Michaela Endres ◽  
Alexander Abbushi ◽  
Mario Cabraja ◽  
Christian Woiciechowsky ◽  
...  
Keyword(s):  
Nucleus Pulposus ◽  
Cell Expansion ◽  
Cell Biology ◽  
3D Culture ◽  
Polyglycolic Acid ◽  
Herniated Disc ◽  
Marker Genes ◽  
Disc Tissue ◽  
Study Results

Object The object of this study was to characterize the regenerative potential of cells isolated from herniated disc tissue obtained during microdiscectomy. The acquired data could help to evaluate the feasibility of these cells for autologous disc cell transplantation. Methods From each of 5 patients (mean age 45 years), tissue from the nucleus pulposus compartment as well as from herniated disc was obtained separately during microdiscectomy of symptomatic herniated lumbar discs. Cells were isolated, and in vitro cell expansion for cells from herniated disc tissue was accomplished using human serum and fibroblast growth factor-2. For 3D culture, expanded cells were loaded in a fibrin-hyaluronan solution on polyglycolic acid scaffolds for 2 weeks. The formation of disc tissue was documented by histological staining of the extracellular matrix as well as by gene expression analysis of typical disc marker genes. Results Cells isolated from herniated disc tissue showed significant signs of dedifferentiation and degeneration in comparison with cells from tissue of the nucleus compartment. With in vitro cell expansion, further dedifferentiation with distinct suppression of major matrix molecules, such as aggrecan and Type II collagen, was observed. Unlike in previous reports of cells from the nucleus compartment, the cells from herniated disc tissue showed only a weak redifferentiation process in 3D culture. However, propidium iodide/fluorescein diacetate staining documented that 3D assembly of these cells in polyglycolic acid scaffolds allows prolonged culture and high viability. Conclusions Study results suggested a very limited regenerative potential for cells harvested from herniated disc tissue. Further research on 2 major aspects in patient selection is suggested before conducting reasonable clinical trials in this matter: 1) diagnostic strategies to predict the regenerative potential of harvested cells at a radiological or cell biology level, and 2) clinical assessment strategies to elucidate the metabolic state of the targeted disc.

Tissue Engineering of a Small Hand Phalanx with a Porously Casted Polylactic Acid–Polyglycolic Acid Copolymer

2006 ◽  
Vol 12 (9) ◽  
pp. 2675-2683 ◽  
Author(s):  
Sargis Sedrakyan ◽  
Zhao Yi Zhou ◽  
Laura Perin ◽  
Kent Leach ◽  
David Mooney ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Polylactic Acid ◽  
Polyglycolic Acid ◽  
Acid Copolymer ◽  
Small Hand

scholarly journals Clinical and Radiographic Evaluation of Nanohydroxyapatite Powder in Combination with Polylactic Acid/Polyglycolic Acid Copolymer as Bone Replacement Graft in the Surgical Treatment of Intrabony Periodontal Defects: A Retrospective Case Series Study

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 269
Author(s):  
Simone Verardi ◽  
Teresa Lombardi ◽  
Claudio Stacchi
Keyword(s):  
Surgical Treatment ◽  
Polylactic Acid ◽  
Case Series ◽  
Polyglycolic Acid ◽  
Retrospective Case ◽  
Acid Copolymer ◽  
Bone Replacement ◽  
Retrospective Case Series ◽  
Bone Replacement Graft

The aim of this retrospective case series was to evaluate the clinical efficacy of nanohydroxyapatite powder (NHA) in combination with polylactic acid/polyglycolic acid copolymer (PLGA) as a bone replacement graft in the surgical treatment of intrabony periodontal defects. Medical charts were screened following inclusion and exclusion criteria. Periodontal parameters and periapical radiographs taken before surgery and at 12-month follow-up were collected. Intra-group comparisons were performed using a two-tailed Wilcoxon signed-rank test. Twenty-five patients (13 males, 12 females, mean age 55.1 ± 10.5 years) were included in the final analysis. Mean probing depth (PD) and clinical attachment level (CAL) at baseline were 8.32 ± 1.41 mm and 9.96 ± 1.69 mm, respectively. Twelve months after surgery, mean PD was 4.04 ± 0.84 mm and CAL was 6.24 ± 1.71 mm. Both PD and CAL variations gave statistically significant results (p < 0.00001). The mean radiographic defect depth was 5.54 ± 1.55 mm and 1.48 ± 1.38 mm at baseline and at 12-month follow-up, respectively (p < 0.0001). This case series, with the limitations inherent in the study design, showed that the combination of NHA and PLGA, used as bone replacement graft in intrabony periodontal defects, may give significant improvements of periodontal parameters at 12-month follow-up.

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